(Circular) (Six-base) MAPSORT of: pFN21AE6370.seq Check: 4544 from: 1 to: 5683 pFN21AE6370 5683 bp With 182 enzymes: SgfI * March 18, 2015 14:51 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2658 2658 Size: 5683 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3493 278 Size: 53 83 186 936 1957 2468 Fragments arranged by size: 2468 1957 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3026 1937 Size: 1089 4594 Acc65I G'GTAC_C Cuts at: 3004 3004 Size: 5683 AclI AA'CG_TT Cuts at: 1563 3810 4183 1563 Size: 2247 373 3063 Fragments arranged by size: 3063 2247 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2492 3746 5130 5398 1632 Size: 860 1254 1384 268 1917 Fragments arranged by size: 1917 1384 1254 860 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5486 AflIII A'CryG_T Cuts at: 1203 2340 2664 4582 5559 1203 Size: 1137 324 1918 977 1327 Fragments arranged by size: 1918 1327 1137 977 324 AgeI A'CCGG_T Cuts at: 4514 4514 Size: 5683 AhdI GACnn_n'nnGTC Cuts at: 4412 4412 Size: 5683 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5683 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2792 1184 Size: 1608 4075 AlwNI CAG_nnn'CTG Cuts at: 2912 3426 4998 2912 Size: 514 1572 3597 Fragments arranged by size: 3597 1572 514 ApaI G_GGCC'C Cuts at: 2033 2043 2747 2937 2033 Size: 10 704 190 4779 Fragments arranged by size: 4779 704 190 10 ApaLI G'TGCA_C Cuts at: 2234 3740 4896 2234 Size: 1506 1156 3021 Fragments arranged by size: 3021 1506 1156 ApoI r'AATT_y Cuts at: 1493 2759 2992 3218 3370 5479 1493 Size: 1266 233 226 152 2109 1697 Fragments arranged by size: 2109 1697 1266 233 226 152 AseI AT'TA_AT Cuts at: 160 1032 4237 160 Size: 872 3205 1606 Fragments arranged by size: 3205 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5683 AvaI C'yCGr_G Cuts at: 1870 1943 2036 2088 2204 3008 1870 Size: 73 93 52 116 804 4545 Fragments arranged by size: 4545 804 116 93 73 52 BamHI G'GATC_C Cuts at: 3013 3380 3013 Size: 367 5316 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2643 3004 4459 618 Size: 325 217 627 856 361 1455 1842 Fragments arranged by size: 1842 1455 856 627 361 325 217 BanII G_rGCy'C Cuts at: 1391 2033 2043 2640 2747 2937 3002 1391 Size: 642 10 597 107 190 65 4072 Fragments arranged by size: 4072 642 597 190 107 65 10 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2480 928 Size: 971 581 4131 Fragments arranged by size: 4131 971 581 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3874 3908 1903 Size: 34 1937 34 3678 Fragments arranged by size: 3678 1937 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3575 4785 3575 Size: 1210 4473 BclI T'GATC_A Cuts at: 1548 1548 Size: 5683 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2963 4294 136 Size: 107 122 71 631 605 1291 1331 1525 Fragments arranged by size: 1525 1331 1291 631 605 122 107 71 BglII A'GATC_T Cuts at: 5678 5678 Size: 5683 BlpI GC'TnA_GC Cuts at: 2901 3100 2901 Size: 199 5484 Bme1580I G_kGCm'C Cuts at: 1790 2033 2043 2238 2747 2937 3744 4900 1790 Size: 243 10 195 509 190 807 1156 2573 Fragments arranged by size: 2573 1156 807 509 243 195 190 10 BmgBI CAC'GTC Cuts at: 1318 2254 2455 1318 Size: 936 201 4546 Fragments arranged by size: 4546 936 201 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2293 2380 3414 Size: 416 53 83 399 24 876 87 1034 Cuts at: 3414 4372 442 Size: 958 1753 Fragments arranged by size: 1753 1034 958 876 416 399 87 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5683 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2752 2752 Size: 5683 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2508 2654 2850 2907 4343 1239 Size: 1269 146 196 57 1436 2579 Fragments arranged by size: 2579 1436 1269 196 146 57 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3808 4673 4971 3808 Size: 865 298 4520 Fragments arranged by size: 4520 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2585 4346 882 Size: 617 1086 1761 2219 Fragments arranged by size: 2219 1761 1086 617 BsaAI yAC'GTr Cuts at: 493 1204 2347 3474 493 Size: 711 1143 1127 2702 Fragments arranged by size: 2702 1143 1127 711 BsaBI GATnn'nnATC Cuts at: 5677 5677 Size: 5683 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2193 3490 3872 4507 Size: 53 83 186 936 660 1297 382 635 Cuts at: 4507 275 Size: 1451 Fragments arranged by size: 1451 1297 936 660 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4116 4514 4788 4935 5346 4116 Size: 398 274 147 411 4453 Fragments arranged by size: 4453 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2439 2469 2627 2657 2878 Size: 30 165 30 1036 30 158 30 221 Cuts at: 2878 2908 1178 Size: 30 3953 Fragments arranged by size: 3953 1036 221 165 158 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5683 BseYI C'CCAG_C Cuts at: 2165 2553 2696 2796 4886 5382 2165 Size: 388 143 100 2090 496 2466 Fragments arranged by size: 2466 2090 496 388 143 100 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2399 2486 2636 1163 Size: 573 663 87 150 4210 Fragments arranged by size: 4210 663 573 150 87 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3133 3894 4922 1540 Size: 275 186 1132 761 1028 2301 Fragments arranged by size: 2301 1132 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2238 2505 3744 3829 4900 2238 Size: 267 1239 85 1071 3021 Fragments arranged by size: 3021 1239 1071 267 85 BsmI GAATG_Cn' Cuts at: 2716 3194 3287 2716 Size: 478 93 5112 Fragments arranged by size: 5112 478 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2033 2043 2238 2505 2640 2747 2937 Size: 399 243 10 195 267 135 107 190 Cuts at: 2937 3002 3744 3829 4900 1391 Size: 65 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 267 243 195 190 135 107 85 65 10 BspEI T'CCGG_A Cuts at: 5346 5346 Size: 5683 BspHI T'CATG_A Cuts at: 3572 5302 3572 Size: 1730 3953 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2215 2658 3038 844 Size: 1371 443 380 3489 Fragments arranged by size: 3489 1371 443 380 BsrBI CCG'CTC Cuts at: 1394 1823 3570 1394 Size: 429 1747 3507 Fragments arranged by size: 3507 1747 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4178 4352 65 Size: 1183 2930 174 1396 Fragments arranged by size: 2930 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4327 4514 1700 Size: 110 2517 187 2869 Fragments arranged by size: 2869 2517 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5683 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5683 BssSI C'ACGA_G Cuts at: 3743 4755 3743 Size: 1012 4671 BstAPI GCAn_nnn'nTGC Cuts at: 794 2614 794 Size: 1820 3863 BstBI TT'CG_AA Cuts at: 4487 4487 Size: 5683 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5683 BstYI r'GATC_y Cuts at: 1972 3013 3380 3765 3782 5234 5320 5332 5678 Size: 1041 367 385 17 1452 86 12 346 Cuts at: 5678 1972 Size: 1977 Fragments arranged by size: 1977 1452 1041 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2363 2363 Size: 5683 BtgI C'CryG_G Cuts at: 513 1065 2004 2219 513 Size: 552 939 215 3977 Fragments arranged by size: 3977 939 552 215 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4944 Fragments arranged by size: 4944 683 56 BtsI GCAGTG_nn' Cuts at: 2302 3199 3993 4013 2302 Size: 897 794 20 3972 Fragments arranged by size: 3972 897 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5683 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5648 DraI TTT'AAA Cuts at: 2987 2987 Size: 5683 DraIII CAC_nnn'GTG Cuts at: 2388 2388 Size: 5683 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4690 809 Size: 507 3374 1802 Fragments arranged by size: 3374 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2216 2955 3130 Size: 54 1425 50 275 18 386 739 175 Cuts at: 3130 4019 5648 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 739 386 275 175 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3130 1537 Size: 275 1318 4090 Fragments arranged by size: 4090 1318 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3613 1353 Size: 2260 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4270 4644 4790 387 Size: 1064 138 2681 374 146 1280 Fragments arranged by size: 2681 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2492 2508 2654 2850 2907 3746 4343 Size: 393 860 16 146 196 57 839 597 Cuts at: 4343 5130 5398 1239 Size: 787 268 1524 Fragments arranged by size: 1524 860 839 787 597 393 268 196 146 57 16 EcoO109I rG'GnC_Cy Cuts at: 2040 2743 2933 2040 Size: 703 190 4790 Fragments arranged by size: 4790 703 190 EcoRI G'AATT_C Cuts at: 2759 2992 3370 2759 Size: 233 378 5072 Fragments arranged by size: 5072 378 233 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2552 2552 Size: 5683 FspI TGC'GCA Cuts at: 2301 4189 2301 Size: 1888 3795 HaeII r_GCGC'y Cuts at: 1678 4830 1678 Size: 3152 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4324 4356 4398 4430 1373 Size: 32 135 32 2752 32 42 32 2626 Fragments arranged by size: 2752 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3027 3273 669 Size: 988 281 1089 246 3079 Fragments arranged by size: 3079 1089 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5683 HpaI GTT'AAC Cuts at: 3273 3273 Size: 5683 KpnI G_GTAC'C Cuts at: 3008 3008 Size: 5683 MfeI C'AATT_G Cuts at: 2548 3282 2548 Size: 734 4949 MluI A'CGCG_T Cuts at: 5559 5559 Size: 5683 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2838 4797 4981 1305 Size: 1533 1959 184 2007 Fragments arranged by size: 2007 1959 1533 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2957 10 Size: 54 1425 343 1125 2736 Fragments arranged by size: 2736 1425 1125 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3641 4000 4159 518 Size: 648 2475 359 159 2042 Fragments arranged by size: 2475 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2297 2470 2626 2772 3099 3776 4924 5169 Size: 691 173 156 146 327 677 1148 245 Cuts at: 5169 1606 Size: 2120 Fragments arranged by size: 2120 1148 691 677 327 245 173 156 146 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4192 Fragments arranged by size: 4192 939 552 NdeI CA'TA_TG Cuts at: 387 2425 387 Size: 2038 3645 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5683 NotI GC'GGCC_GC Cuts at: 3130 3130 Size: 5683 NspI r_CATG'y Cuts at: 1132 1229 2344 2668 2689 3041 4586 1132 Size: 97 1115 324 21 352 1545 2229 Fragments arranged by size: 2229 1545 1115 352 324 97 21 PasI CC'CwG_GG Cuts at: 1348 2050 1348 Size: 702 4981 PciI A'CATG_T Cuts at: 2340 2664 4582 2340 Size: 324 1918 3441 Fragments arranged by size: 3441 1918 324 PflMI CCAn_nnn'nTGG Cuts at: 1108 2553 1108 Size: 1445 4238 PfoI T'CCnGG_A Cuts at: 2631 2631 Size: 5683 PmeI GTTT'AAAC Cuts at: 2987 2987 Size: 5683 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3752 3784 5265 5297 3752 Size: 32 1481 32 4138 Fragments arranged by size: 4138 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 2665 1613 Size: 1052 4631 PsiI TTA'TAA Cuts at: 3253 3253 Size: 5683 PspOMI G'GGCC_C Cuts at: 2029 2039 2743 2933 2029 Size: 10 704 190 4779 Fragments arranged by size: 4779 704 190 10 PstI C_TGCA'G Cuts at: 830 2610 3035 830 Size: 1780 425 3478 Fragments arranged by size: 3478 1780 425 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5683 PvuII CAG'CTG Cuts at: 2297 2470 2772 2297 Size: 173 302 5208 Fragments arranged by size: 5208 302 173 SalI G'TCGA_C Cuts at: 1936 3025 1936 Size: 1089 4594 SbfI CC_TGCA'GG Cuts at: 3035 3035 Size: 5683 ScaI AGT'ACT Cuts at: 2282 3931 5643 2282 Size: 1649 1712 2322 Fragments arranged by size: 2322 1712 1649 SfcI C'TryA_G Cuts at: 826 1045 2606 3031 4166 4847 5038 826 Size: 219 1561 425 1135 681 191 1471 Fragments arranged by size: 1561 1471 1135 681 425 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5683 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5683 SmaI CCC'GGG Cuts at: 1872 2090 3010 1872 Size: 218 920 4545 Fragments arranged by size: 4545 920 218 SmlI C'TyrA_G Cuts at: 820 1017 1943 3787 4688 4950 820 Size: 197 926 1844 901 262 1553 Fragments arranged by size: 1844 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 2347 3474 493 Size: 1854 1127 2702 Fragments arranged by size: 2702 1854 1127 SpeI A'CTAG_T Cuts at: 152 2572 152 Size: 2420 3263 SphI G_CATG'C Cuts at: 1132 3041 1132 Size: 1909 3774 SspI AAT'ATT Cuts at: 5 52 3607 5 Size: 47 3555 2081 Fragments arranged by size: 3555 2081 47 StuI AGG'CCT Cuts at: 1517 2247 2352 1517 Size: 730 105 4848 Fragments arranged by size: 4848 730 105 StyI C'CwwG_G Cuts at: 513 1065 2004 3122 513 Size: 552 939 1118 3074 Fragments arranged by size: 3074 1118 939 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2275 2399 3710 3727 3880 4065 4510 Size: 1255 695 124 1311 17 153 185 445 Cuts at: 4510 325 Size: 1498 Fragments arranged by size: 1498 1311 1255 695 445 185 153 124 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2280 3929 5641 Size: 274 80 33 51 1443 303 1649 1712 Cuts at: 5641 96 Size: 138 Fragments arranged by size: 1712 1649 1443 303 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2439 2471 2627 2659 2878 2910 5643 5675 2439 Size: 32 156 32 219 32 2733 32 2447 Fragments arranged by size: 2733 2447 219 156 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5683 XbaI T'CTAG_A Cuts at: 3019 3019 Size: 5683 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5683 XmaI C'CCGG_G Cuts at: 1870 2088 3008 1870 Size: 218 920 4545 Fragments arranged by size: 4545 920 218 XmnI GAAnn'nnTTC Cuts at: 3812 3812 Size: 5683 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3491 276 Size: 53 83 186 936 1957 2468 Fragments arranged by size: 2468 1957 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI Bpu10I BsiWI BsmBI BstXI BstZ17I EcoICRI EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI