################################## # NO CUT ENZYMES ( 111 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcsI AflII AgeI AhdI Aor51HI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvrII BamHI BbeI BbrPI BciVI BfaI BfrI BlnI BsaBI BsaI BsaWI BseAI BsiWI BsmI Bsp1407I BspDI BspEI BspHI BsrDI BsrGI BssHII BssSI Bst1107I BstBI BstEII BstPI BstZ17I ClaI CpoI DraI DrdI Eam1105I Eco47III EcoO65I EcoRI EcoRV EcoT22I FseI HindIII HpaI KasI KpnI KspI MaeI MamI MfeI MluI MroI MseI MspA1I MunI NarI NdeI NheI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI PvuII RcaI RsrII SacII SalI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SnaBI SpeI Sse8387I SspBI SwaI Tru9I Tsp509I Tth111I Van91I XbaI XhoI XmnI ################################### # ONE CUT ENZYMES ( 37 enzymes) # ################################### AcyI 1669 GACGCC G[GA]^CG[CT]C AfaI 1080 GTAC GT^AC AflIII 1320 ACATGT A^C[GA][CT]GT Alw44I 1030 GTGCAC G^TGCAC ApaLI 1030 GTGCAC G^TGCAC AviII 791 TGCGCA TGC^GCA BbsI 1666 GAAGAC GAAGAC(2/6) BcgI 768 CGAGGCCAATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BclI 107 TGATCA T^GATCA BpmI 1210 CTGGAG CTGGAG(16/14) BpuAI 1666 GAAGAC GAAGAC(2/6) BsaAI 355 TACGTG [CT]AC^GT[GA] BsaHI 1669 GACGCC G[GA]^CG[CT]C BsmBI 1792 CGTCTC CGTCTC(1/5) BspLU11I 1320 ACATGT A^CATGT DraIII 1501 CACAAGGTG CAC[ACGT][ACGT][ACGT]^GTG EcoT14I 464 CCATGG C^C[AT][AT]GG FbaI 107 TGATCA T^GATCA FspI 791 TGCGCA TGC^GCA HaeII 306 AGCGCC [GA]GCGC^[CT] Hin1I 1669 GACGCC G[GA]^CG[CT]C HincII 1114 GTCAAC GT[CT]^[GA]AC HindII 1114 GTCAAC GT[CT]^[GA]AC NaeI 635 GCCGGC GCC^GGC NcoI 464 CCATGG C^CATGG NgoMIV 635 GCCGGC G^CCGGC NotI 1052 GCGGCCGC GC^GGCCGC PleI 451 GACTC GAGTC(4/5) RsaI 1080 GTAC GT^AC SacI 1306 GAGCTC GAGCT^C SapI 702 GAAGAGC GCTCTTC(1/4) SmaI 602 CCCGGG CCC^GGG SmlI 823 CTCAAG C^T[CT][GA]AG SspI 197 AATATT AAT^ATT StyI 464 CCATGG C^C[AT][AT]GG XmaCI 602 CCCGGG C^CCGGG XmaI 602 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 27 enzymes) # ################################### AlwNI 344 CAGCTCCTG CAG[ACGT][ACGT][ACGT]^CTG 835 CAGATGCTG AocI 725 CCTCAGG CC^T[ACGT]AGG 1425 CCTGAGG BanI 615 GGTGCC G^G[CT][GA]CC 1787 GGCACC BbvCI 1191 GCTGAGG CCTCAGC(-5/-2) 1782 GCTGAGG BglI 352 GCCTACGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 541 GCCCGGCAGGC BglII 884 AGATCT A^GATCT 1397 AGATCT BsgI 1825 CTGCAC GTGCAG(16/14) 172 GTGCAG BsiEI 62 CGGCCG CG[GA][CT]^CG 1053 CGGCCG BsrFI 635 GCCGGC [GA]^CCGG[CT] 1642 ACCGGC BstAPI 794 GCACGCTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 1913 GCAACTACTGC Bsu36I 725 CCTCAGG CC^T[ACGT]AGG 1425 CCTGAGG Cfr10I 635 GCCGGC [GA]^CCGG[CT] 1642 ACCGGC EagI 62 CGGCCG C^GGCCG 1053 CGGCCG EarI 279 CTCTTC CTCTTC(1/4) 702 GAAGAG EclXI 62 CGGCCG C^GGCCG 1053 CGGCCG Eco52I 62 CGGCCG C^GGCCG 1053 CGGCCG Eco81I 725 CCTCAGG CC^T[ACGT]AGG 1425 CCTGAGG HgaI 585 GACGC GACGC(5/10) 1669 GACGC HphI 1553 TCACC GGTGA(8/7) 1640 TCACC Ksp632I 279 CTCTTC CTCTTC(1/4) 702 GAAGAG MaeII 356 ACGT A^CGT 1187 ACGT MaeIII 622 GTGAC ^GT[ACGT]AC 1217 GTGAC PpuMI 719 AGGACCC [GA]G^G[AT]CC[CT] 1256 GGGACCT SphI 252 GCATGC GCATG^C 597 GCATGC TfiI 1386 GATTC G^A[AT]TC 1770 GATTC Tsp45I 622 GTGAC ^GT[GC]AC 1217 GTGAC XcmI 498 CCAATCCCCTGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 1685 CCATCCTCATCCTGG ######################################### # 3 OR MORE CUT ENZYMES ( 83 enzymes) # ######################################### AccII AciI AluI AlwI ApaI AspHI AvaI AvaII BalI BanII BbvI BcnI BlpI BmyI Bpu1102I BsaJI BseRI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspMI BsrBI BsrI BssKI BstF5I BstNI BstUI BstXI BstYI Cac8I CelII CfoI Cfr13I DdeI DpnII DraII DsaI EaeI EcoNI EcoO109I EcoRII FauI Fnu4HI FokI HaeIII HapII HhaI HinP1I HinfI HpaII ItaI MboI MboII MflI MluNI MnlI MscI MslI MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV NspI PstI Sau3AI Sau96I ScrFI SfaNI SfcI StuI TaqI TseI TspRI TthHB8I XhoII