(Circular) (Six-base) MAPSORT of: pFN21AE0994.seq Check: 3051 from: 1 to: 6646 pFN21AE0994 6646 bp With 182 enzymes: SgfI * March 28, 2012 12:30 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2091 3022 2091 Size: 931 5715 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4456 278 Size: 53 83 186 936 2920 2468 Fragments arranged by size: 2920 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3989 1937 Size: 2052 4594 Acc65I G'GTAC_C Cuts at: 3967 3967 Size: 6646 AclI AA'CG_TT Cuts at: 1563 4773 5146 1563 Size: 3210 373 3063 Fragments arranged by size: 3210 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2271 2951 3290 3893 4709 6093 6361 1632 Size: 639 680 339 603 816 1384 268 1917 Fragments arranged by size: 1917 1384 816 680 639 603 339 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6449 AflIII A'CryG_T Cuts at: 1203 3325 5545 6522 1203 Size: 2122 2220 977 1327 Fragments arranged by size: 2220 2122 1327 977 AgeI A'CCGG_T Cuts at: 5477 5477 Size: 6646 AhdI GACnn_n'nnGTC Cuts at: 5375 5375 Size: 6646 AleI CACnn'nnGTG Cuts at: 1166 2144 1166 Size: 978 5668 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6646 AlwNI CAG_nnn'CTG Cuts at: 2354 2845 4389 5961 2354 Size: 491 1544 1572 3039 Fragments arranged by size: 3039 1572 1544 491 ApaI G_GGCC'C Cuts at: 2131 2348 2969 2131 Size: 217 621 5808 Fragments arranged by size: 5808 621 217 ApaLI G'TGCA_C Cuts at: 3035 4703 5859 3035 Size: 1668 1156 3822 Fragments arranged by size: 3822 1668 1156 ApoI r'AATT_y Cuts at: 1493 3955 4181 4333 6442 1493 Size: 2462 226 152 2109 1697 Fragments arranged by size: 2462 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 5200 160 Size: 872 4168 1606 Fragments arranged by size: 4168 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6646 AvaI C'yCGr_G Cuts at: 1870 1943 2567 2607 3176 3812 3971 1870 Size: 73 624 40 569 636 159 4545 Fragments arranged by size: 4545 636 624 569 159 73 40 BamHI G'GATC_C Cuts at: 3976 4343 3976 Size: 367 6279 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2620 3792 3967 5422 618 Size: 325 217 627 833 1172 175 1455 1842 Fragments arranged by size: 1842 1455 1172 833 627 325 217 175 BanII G_rGCy'C Cuts at: 1391 2131 2348 2969 3315 3381 3851 3965 1391 Size: 740 217 621 346 66 470 114 4072 Fragments arranged by size: 4072 740 621 470 346 217 114 66 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3678 928 Size: 971 1779 3896 Fragments arranged by size: 3896 1779 971 BbvCI CC'TCA_GC Cuts at: 3197 3788 3197 Size: 591 6055 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2762 2796 4837 4871 1903 Size: 34 825 34 2041 34 3678 Fragments arranged by size: 3678 2041 825 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4538 5748 4538 Size: 1210 5436 BclI T'GATC_A Cuts at: 1548 2112 1548 Size: 564 6082 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2363 2552 5257 Size: 107 122 71 631 605 691 189 2705 Cuts at: 5257 136 Size: 1525 Fragments arranged by size: 2705 1525 691 631 605 189 122 107 71 BglII A'GATC_T Cuts at: 2889 3402 6641 2889 Size: 513 3239 2894 Fragments arranged by size: 3239 2894 513 BlpI GC'TnA_GC Cuts at: 2594 2846 3380 4063 2594 Size: 252 534 683 5177 Fragments arranged by size: 5177 683 534 252 Bme1580I G_kGCm'C Cuts at: 1790 2131 2348 2689 2969 3039 3250 4707 5863 Size: 341 217 341 280 70 211 1457 1156 Cuts at: 5863 1790 Size: 2573 Fragments arranged by size: 2573 1457 1156 341 341 280 217 211 70 BmgBI CAC'GTC Cuts at: 1318 3193 1318 Size: 1875 4771 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4377 5335 442 Size: 416 53 83 399 24 2960 958 1753 Fragments arranged by size: 2960 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6646 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2746 2746 Size: 6646 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3236 5306 1239 Size: 1997 2070 2579 Fragments arranged by size: 2579 2070 1997 Bpu10I CC'TnA_GC Cuts at: 2556 3197 3788 2556 Size: 641 591 5414 Fragments arranged by size: 5414 641 591 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2813 4771 5636 5934 2813 Size: 1958 865 298 3525 Fragments arranged by size: 3525 1958 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5309 882 Size: 617 3810 2219 Fragments arranged by size: 3810 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2362 4437 493 Size: 711 1158 2075 2702 Fragments arranged by size: 2702 2075 1158 711 BsaBI GATnn'nnATC Cuts at: 6640 6640 Size: 6646 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3675 4453 4835 5470 Size: 53 83 186 936 2142 778 382 635 Cuts at: 5470 275 Size: 1451 Fragments arranged by size: 2142 1451 936 778 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5079 5477 5751 5898 6309 5079 Size: 398 274 147 411 5416 Fragments arranged by size: 5416 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6421 Fragments arranged by size: 6421 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2398 3014 3920 1186 Size: 1212 616 906 3912 Fragments arranged by size: 3912 1212 906 616 BseYI C'CCAG_C Cuts at: 2347 2749 2833 3498 3939 5849 6345 2347 Size: 402 84 665 441 1910 496 2648 Fragments arranged by size: 2648 1910 665 496 441 402 84 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2198 3815 1163 Size: 573 462 1617 3994 Fragments arranged by size: 3994 1617 573 462 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2070 3061 4096 4857 5885 1540 Size: 275 186 69 991 1035 761 1028 2301 Fragments arranged by size: 2301 1035 1028 991 761 275 186 69 BsiHKAI G_wGCw'C Cuts at: 3039 3183 3315 3752 4707 4792 5863 3039 Size: 144 132 437 955 85 1071 3822 Fragments arranged by size: 3822 1071 955 437 144 132 85 BsmI GAATG_Cn' Cuts at: 4157 4250 4157 Size: 93 6553 BsmBI CGTCTCn'nnnn_ Cuts at: 3803 3803 Size: 6646 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2131 2348 2689 2969 3039 3183 3250 Size: 399 341 217 341 280 70 144 67 Cuts at: 3250 3315 3381 3752 3851 3965 4707 4792 5863 Size: 65 66 371 99 114 742 85 1071 Cuts at: 5863 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 399 371 341 341 280 217 144 114 99 85 70 67 66 65 BspEI T'CCGG_A Cuts at: 6309 6309 Size: 6646 BspHI T'CATG_A Cuts at: 4535 6265 4535 Size: 1730 4916 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2091 3022 3273 3934 4001 844 Size: 1247 931 251 661 67 3489 Fragments arranged by size: 3489 1247 931 661 251 67 BsrBI CCG'CTC Cuts at: 1394 1823 2297 2614 2788 2899 3004 4533 1394 Size: 429 474 317 174 111 105 1529 3507 Fragments arranged by size: 3507 1529 474 429 317 174 111 105 BsrDI GCAATG_nn' Cuts at: 65 1248 5141 5315 65 Size: 1183 3893 174 1396 Fragments arranged by size: 3893 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2640 3647 5290 5477 1700 Size: 110 830 1007 1643 187 2869 Fragments arranged by size: 2869 1643 1007 830 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6646 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6646 BssSI C'ACGA_G Cuts at: 4706 5718 4706 Size: 1012 5634 BstAPI GCAn_nnn'nTGC Cuts at: 794 2805 3924 794 Size: 2011 1119 3516 Fragments arranged by size: 3516 2011 1119 BstBI TT'CG_AA Cuts at: 5450 5450 Size: 6646 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6646 BstXI CCAn_nnnn'nTGG Cuts at: 2035 2146 3089 3343 2035 Size: 111 943 254 5338 Fragments arranged by size: 5338 943 254 111 BstYI r'GATC_y Cuts at: 1972 2889 3078 3402 3551 3762 3976 4343 4728 Size: 917 189 324 149 211 214 367 385 Cuts at: 4728 4745 6197 6283 6295 6641 1972 Size: 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 917 385 367 346 324 214 211 189 149 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2731 3431 2731 Size: 700 5946 BtgI C'CryG_G Cuts at: 513 1065 2004 2070 2139 2469 2667 513 Size: 552 939 66 69 330 198 4492 Fragments arranged by size: 4492 939 552 330 198 69 66 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5907 Fragments arranged by size: 5907 683 56 BtsI GCAGTG_nn' Cuts at: 4162 4956 4976 4162 Size: 794 20 5832 Fragments arranged by size: 5832 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6646 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2797 2832 529 Size: 35 2233 35 4343 Fragments arranged by size: 4343 2233 35 35 DraI TTT'AAA Cuts at: 3950 3950 Size: 6646 DraIII CAC_nnn'GTG Cuts at: 3511 3511 Size: 6646 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5653 809 Size: 507 4337 1802 Fragments arranged by size: 4337 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2067 2121 2364 Size: 54 1425 50 275 18 237 54 243 Cuts at: 2364 2398 2638 2646 2664 3058 3069 3132 4093 Size: 34 240 8 18 394 11 63 961 Cuts at: 4093 4982 6611 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 961 889 394 275 243 240 237 63 54 54 50 43 34 18 18 11 8 EagI C'GGCC_G Cuts at: 1537 1812 2067 3058 4093 1537 Size: 275 255 991 1035 4090 Fragments arranged by size: 4090 1035 991 275 255 EarI CTCTTCn'nnn_ Cuts at: 1353 2290 2702 4576 1353 Size: 937 412 1874 3423 Fragments arranged by size: 3423 1874 937 412 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5233 5607 5753 387 Size: 1064 138 3644 374 146 1280 Fragments arranged by size: 3644 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3313 3313 Size: 6646 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2271 2951 3236 3290 3893 4709 5306 Size: 393 639 680 285 54 603 816 597 Cuts at: 5306 6093 6361 1239 Size: 787 268 1524 Fragments arranged by size: 1524 816 787 680 639 603 597 393 285 268 54 EcoNI CCTnn'n_nnAGG Cuts at: 2771 3269 3365 3704 2771 Size: 498 96 339 5713 Fragments arranged by size: 5713 498 339 96 EcoO109I rG'GnC_Cy Cuts at: 2127 2128 2344 2518 2725 2966 3262 2127 Size: 1 216 174 207 241 296 5511 Fragments arranged by size: 5511 296 241 216 207 174 1 EcoRI G'AATT_C Cuts at: 3955 4333 3955 Size: 378 6268 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3898 3898 Size: 6646 FspI TGC'GCA Cuts at: 2798 5152 2798 Size: 2354 4292 FspAI rTGC'GCAy Cuts at: 2798 2798 Size: 6646 HaeII r_GCGC'y Cuts at: 1678 2315 5793 1678 Size: 637 3478 2531 Fragments arranged by size: 3478 2531 637 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2013 2045 2447 2479 2932 Size: 32 135 32 441 32 402 32 453 Cuts at: 2932 2964 3332 3364 3781 3813 5287 5319 5361 Size: 32 368 32 417 32 1474 32 42 Cuts at: 5361 5393 1373 Size: 32 2626 Fragments arranged by size: 2626 1474 453 441 417 402 368 135 42 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3121 3990 4236 669 Size: 988 281 1183 869 246 3079 Fragments arranged by size: 3079 1183 988 869 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6646 HpaI GTT'AAC Cuts at: 4236 4236 Size: 6646 KpnI G_GTAC'C Cuts at: 3971 3971 Size: 6646 MfeI C'AATT_G Cuts at: 4245 4245 Size: 6646 MluI A'CGCG_T Cuts at: 6522 6522 Size: 6646 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2967 3504 5760 5944 1305 Size: 1662 537 2256 184 2007 Fragments arranged by size: 2256 2007 1662 537 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2366 2648 3071 10 Size: 54 1425 343 534 282 423 3585 Fragments arranged by size: 3585 1425 534 423 343 282 54 MslI CAynn'nnrTG Cuts at: 518 1166 2030 2033 2144 3134 3632 3684 4604 Size: 648 864 3 111 990 498 52 920 Cuts at: 4604 4963 5122 518 Size: 359 159 2042 Fragments arranged by size: 2042 990 920 864 648 498 359 159 111 52 3 MspA1I CmG'CkG Cuts at: 1606 4062 4739 5887 6132 1606 Size: 2456 677 1148 245 2120 Fragments arranged by size: 2456 2120 1148 677 245 NaeI GCC'GGC Cuts at: 2642 2642 Size: 6646 NcoI C'CATG_G Cuts at: 513 1065 2004 2469 513 Size: 552 939 465 4690 Fragments arranged by size: 4690 939 552 465 NdeI CA'TA_TG Cuts at: 387 387 Size: 6646 NgoMIV G'CCGG_C Cuts at: 2640 2640 Size: 6646 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6646 NotI GC'GGCC_GC Cuts at: 3058 4093 3058 Size: 1035 5611 NspI r_CATG'y Cuts at: 1132 1229 2164 2261 2606 3157 3329 4004 5549 Size: 97 935 97 345 551 172 675 1545 Cuts at: 5549 1132 Size: 2229 Fragments arranged by size: 2229 1545 935 675 551 345 172 97 97 PasI CC'CwG_GG Cuts at: 1348 2323 1348 Size: 975 5671 PciI A'CATG_T Cuts at: 3325 5545 3325 Size: 2220 4426 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6646 PfoI T'CCnGG_A Cuts at: 2853 2853 Size: 6646 PmeI GTTT'AAAC Cuts at: 3950 3950 Size: 6646 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3623 3655 4715 4747 6228 6260 3623 Size: 32 1060 32 1481 32 4009 Fragments arranged by size: 4009 1481 1060 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2725 3262 2725 Size: 537 6109 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6646 PsiI TTA'TAA Cuts at: 4216 4216 Size: 6646 PspOMI G'GGCC_C Cuts at: 2127 2344 2965 2127 Size: 217 621 5808 Fragments arranged by size: 5808 621 217 PstI C_TGCA'G Cuts at: 830 2088 2136 3320 3998 830 Size: 1258 48 1184 678 3478 Fragments arranged by size: 3478 1258 1184 678 48 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6646 SacI G_AGCT'C Cuts at: 3315 3315 Size: 6646 SalI G'TCGA_C Cuts at: 1936 3988 1936 Size: 2052 4594 SapI GCTCTTCn'nnn_ Cuts at: 2702 2702 Size: 6646 SbfI CC_TGCA'GG Cuts at: 3998 3998 Size: 6646 ScaI AGT'ACT Cuts at: 4894 6606 4894 Size: 1712 4934 SfcI C'TryA_G Cuts at: 826 1045 2084 2132 3316 3913 3994 5129 5810 Size: 219 1039 48 1184 597 81 1135 681 Cuts at: 5810 6001 826 Size: 191 1471 Fragments arranged by size: 1471 1184 1135 1039 681 597 219 191 81 48 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6646 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6646 SmaI CCC'GGG Cuts at: 1872 2609 3973 1872 Size: 737 1364 4545 Fragments arranged by size: 4545 1364 737 SmlI C'TyrA_G Cuts at: 820 1017 1943 2828 4750 5651 5913 820 Size: 197 926 885 1922 901 262 1553 Fragments arranged by size: 1922 1553 926 901 885 262 197 SnaBI TAC'GTA Cuts at: 493 4437 493 Size: 3944 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6646 SphI G_CATG'C Cuts at: 1132 2261 2606 4004 1132 Size: 1129 345 1398 3774 Fragments arranged by size: 3774 1398 1129 345 SspI AAT'ATT Cuts at: 5 52 2204 4570 5 Size: 47 2152 2366 2081 Fragments arranged by size: 2366 2152 2081 47 StuI AGG'CCT Cuts at: 1517 2046 2405 3168 3710 3911 1517 Size: 529 359 763 542 201 4252 Fragments arranged by size: 4252 763 542 529 359 201 StyI C'CwwG_G Cuts at: 513 1065 2004 2469 4085 513 Size: 552 939 465 1616 3074 Fragments arranged by size: 3074 1616 939 552 465 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4673 4690 4843 5028 5473 325 Size: 1255 3093 17 153 185 445 1498 Fragments arranged by size: 3093 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4892 6604 96 Size: 274 80 33 51 1443 2915 1712 138 Fragments arranged by size: 2915 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6606 6638 6606 Size: 32 6614 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6646 XbaI T'CTAG_A Cuts at: 3982 3982 Size: 6646 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2510 3697 2510 Size: 1187 5459 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6646 XmaI C'CCGG_G Cuts at: 1870 2607 3971 1870 Size: 737 1364 4545 Fragments arranged by size: 4545 1364 737 XmnI GAAnn'nnTTC Cuts at: 4775 4775 Size: 6646 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4454 276 Size: 53 83 186 936 2920 2468 Fragments arranged by size: 2920 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI FspAI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbeI BfrBI BsiWI BstZ17I EcoRV FseI KasI NarI NruI NsiI PacI PmlI PspXI PsrI PvuII RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI