(Linear) (Six-base) MAPSORT of: KIEE0994.seq Check: 1354 from: 1 to: 1941 KIEE0994.orf With 182 enzymes: SgfI * March 22, 2012 15:50 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 86 1017 1941 Size: 86 931 924 Fragments arranged by size: 931 924 86 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 266 946 1285 1888 1941 Size: 266 680 339 603 53 Fragments arranged by size: 680 603 339 266 53 AflIII A'CryG_T Cuts at: 0 1320 1941 Size: 1320 621 AleI CACnn'nnGTG Cuts at: 0 139 1941 Size: 139 1802 AlwNI CAG_nnn'CTG Cuts at: 0 349 840 1941 Size: 349 491 1101 Fragments arranged by size: 1101 491 349 ApaI G_GGCC'C Cuts at: 0 126 343 964 1941 Size: 126 217 621 977 Fragments arranged by size: 977 621 217 126 ApaLI G'TGCA_C Cuts at: 0 1030 1941 Size: 1030 911 AvaI C'yCGr_G Cuts at: 0 562 602 1171 1807 1941 Size: 562 40 569 636 134 Fragments arranged by size: 636 569 562 134 40 BanI G'GyrC_C Cuts at: 0 615 1787 1941 Size: 615 1172 154 Fragments arranged by size: 1172 615 154 BanII G_rGCy'C Cuts at: 0 126 343 964 1310 1376 1846 1941 Size: 126 217 621 346 66 470 95 Fragments arranged by size: 621 470 346 217 126 95 66 BbsI GAAGACnn'nnnn_ Cuts at: 0 1673 1941 Size: 1673 268 BbvCI CC'TCA_GC Cuts at: 0 1192 1783 1941 Size: 1192 591 158 Fragments arranged by size: 1192 591 158 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 757 791 1941 Size: 757 34 1150 Fragments arranged by size: 1150 757 34 BclI T'GATC_A Cuts at: 0 107 1941 Size: 107 1834 BglI GCCn_nnn'nGGC Cuts at: 0 358 547 1941 Size: 358 189 1394 Fragments arranged by size: 1394 358 189 BglII A'GATC_T Cuts at: 0 884 1397 1941 Size: 884 513 544 Fragments arranged by size: 884 544 513 BlpI GC'TnA_GC Cuts at: 0 589 841 1375 1941 Size: 589 252 534 566 Fragments arranged by size: 589 566 534 252 Bme1580I G_kGCm'C Cuts at: 0 126 343 684 964 1034 1245 1941 Size: 126 217 341 280 70 211 696 Fragments arranged by size: 696 341 280 217 211 126 70 BmgBI CAC'GTC Cuts at: 0 1188 1941 Size: 1188 753 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 741 1941 Size: 741 1200 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1231 1941 Size: 1231 710 Bpu10I CC'TnA_GC Cuts at: 0 551 1192 1783 1941 Size: 551 641 591 158 Fragments arranged by size: 641 591 551 158 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 808 1941 Size: 808 1133 BsaAI yAC'GTr Cuts at: 0 357 1941 Size: 357 1584 BsaHI Gr'CG_yC Cuts at: 0 1670 1941 Size: 1670 271 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 393 1009 1915 1941 Size: 393 616 906 26 Fragments arranged by size: 906 616 393 26 BseYI C'CCAG_C Cuts at: 0 342 744 828 1493 1934 1941 Size: 342 402 84 665 441 7 Fragments arranged by size: 665 441 402 342 84 7 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 193 1810 1941 Size: 193 1617 131 Fragments arranged by size: 1617 193 131 BsiEI CG_ry'CG Cuts at: 0 65 1056 1941 Size: 65 991 885 Fragments arranged by size: 991 885 65 BsiHKAI G_wGCw'C Cuts at: 0 1034 1178 1310 1747 1941 Size: 1034 144 132 437 194 Fragments arranged by size: 1034 437 194 144 132 BsmBI CGTCTCn'nnnn_ Cuts at: 0 1798 1941 Size: 1798 143 Bsp1286I G_dGCh'C Cuts at: 0 126 343 684 964 1034 1178 1245 1310 Size: 126 217 341 280 70 144 67 65 Cuts at: 1310 1376 1747 1846 1941 Size: 66 371 99 95 Fragments arranged by size: 371 341 280 217 144 126 99 95 70 67 66 65 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 86 1017 1268 1941 Size: 86 931 251 673 Fragments arranged by size: 931 673 251 86 BsrBI CCG'CTC Cuts at: 0 292 609 783 894 999 1941 Size: 292 317 174 111 105 942 Fragments arranged by size: 942 317 292 174 111 105 BsrFI r'CCGG_y Cuts at: 0 635 1642 1941 Size: 635 1007 299 Fragments arranged by size: 1007 635 299 BstAPI GCAn_nnn'nTGC Cuts at: 0 800 1919 1941 Size: 800 1119 22 Fragments arranged by size: 1119 800 22 BstXI CCAn_nnnn'nTGG Cuts at: 0 30 141 1084 1338 1941 Size: 30 111 943 254 603 Fragments arranged by size: 943 603 254 111 30 BstYI r'GATC_y Cuts at: 0 884 1073 1397 1546 1757 1941 Size: 884 189 324 149 211 184 Fragments arranged by size: 884 324 211 189 184 149 Bsu36I CC'TnA_GG Cuts at: 0 726 1426 1941 Size: 726 700 515 Fragments arranged by size: 726 700 515 BtgI C'CryG_G Cuts at: 0 65 134 464 662 1941 Size: 65 69 330 198 1279 Fragments arranged by size: 1279 330 198 69 65 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 792 827 1941 Size: 792 35 1114 Fragments arranged by size: 1114 792 35 DraIII CAC_nnn'GTG Cuts at: 0 1506 1941 Size: 1506 435 EaeI y'GGCC_r Cuts at: 0 62 116 359 393 633 641 659 1053 Size: 62 54 243 34 240 8 18 394 Cuts at: 1053 1064 1127 1941 Size: 11 63 814 Fragments arranged by size: 814 394 243 240 63 62 54 34 18 11 8 EagI C'GGCC_G Cuts at: 0 62 1053 1941 Size: 62 991 888 Fragments arranged by size: 991 888 62 EarI CTCTTCn'nnn_ Cuts at: 0 285 697 1941 Size: 285 412 1244 Fragments arranged by size: 1244 412 285 EcoICRI GAG'CTC Cuts at: 0 1308 1941 Size: 1308 633 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 266 946 1231 1285 1888 1941 Size: 266 680 285 54 603 53 Fragments arranged by size: 680 603 285 266 54 53 EcoNI CCTnn'n_nnAGG Cuts at: 0 766 1264 1360 1699 1941 Size: 766 498 96 339 242 Fragments arranged by size: 766 498 339 242 96 EcoO109I rG'GnC_Cy Cuts at: 0 122 123 339 513 720 961 1257 1941 Size: 122 1 216 174 207 241 296 684 Fragments arranged by size: 684 296 241 216 207 174 122 1 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 1893 1941 Size: 1893 48 FspI TGC'GCA Cuts at: 0 793 1941 Size: 793 1148 FspAI rTGC'GCAy Cuts at: 0 793 1941 Size: 793 1148 HaeII r_GCGC'y Cuts at: 0 310 1941 Size: 310 1631 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 8 40 442 474 927 959 1327 1359 Size: 8 32 402 32 453 32 368 32 Cuts at: 1359 1776 1808 1941 Size: 417 32 133 Fragments arranged by size: 453 417 402 368 133 32 32 32 32 32 8 HincII GTy'rAC Cuts at: 0 1116 1941 Size: 1116 825 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 962 1499 1941 Size: 962 537 442 Fragments arranged by size: 962 537 442 MscI TGG'CCA Cuts at: 0 361 643 1066 1941 Size: 361 282 423 875 Fragments arranged by size: 875 423 361 282 MslI CAynn'nnrTG Cuts at: 0 25 28 139 1129 1627 1679 1941 Size: 25 3 111 990 498 52 262 Fragments arranged by size: 990 498 262 111 52 25 3 NaeI GCC'GGC Cuts at: 0 637 1941 Size: 637 1304 NcoI C'CATG_G Cuts at: 0 464 1941 Size: 464 1477 NgoMIV G'CCGG_C Cuts at: 0 635 1941 Size: 635 1306 NotI GC'GGCC_GC Cuts at: 0 1053 1941 Size: 1053 888 NspI r_CATG'y Cuts at: 0 159 256 601 1152 1324 1941 Size: 159 97 345 551 172 617 Fragments arranged by size: 617 551 345 172 159 97 PasI CC'CwG_GG Cuts at: 0 318 1941 Size: 318 1623 PciI A'CATG_T Cuts at: 0 1320 1941 Size: 1320 621 PfoI T'CCnGG_A Cuts at: 0 848 1941 Size: 848 1093 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 1618 1650 1941 Size: 1618 32 291 Fragments arranged by size: 1618 291 32 PpuMI rG'GwC_Cy Cuts at: 0 720 1257 1941 Size: 720 537 684 Fragments arranged by size: 720 684 537 PspOMI G'GGCC_C Cuts at: 0 122 339 960 1941 Size: 122 217 621 981 Fragments arranged by size: 981 621 217 122 PstI C_TGCA'G Cuts at: 0 83 131 1315 1941 Size: 83 48 1184 626 Fragments arranged by size: 1184 626 83 48 SacI G_AGCT'C Cuts at: 0 1310 1941 Size: 1310 631 SapI GCTCTTCn'nnn_ Cuts at: 0 697 1941 Size: 697 1244 SfcI C'TryA_G Cuts at: 0 79 127 1311 1908 1941 Size: 79 48 1184 597 33 Fragments arranged by size: 1184 597 79 48 33 SmaI CCC'GGG Cuts at: 0 604 1941 Size: 604 1337 SmlI C'TyrA_G Cuts at: 0 823 1941 Size: 823 1118 SphI G_CATG'C Cuts at: 0 256 601 1941 Size: 256 345 1340 Fragments arranged by size: 1340 345 256 SspI AAT'ATT Cuts at: 0 199 1941 Size: 199 1742 StuI AGG'CCT Cuts at: 0 41 400 1163 1705 1906 1941 Size: 41 359 763 542 201 35 Fragments arranged by size: 763 542 359 201 41 35 StyI C'CwwG_G Cuts at: 0 464 1941 Size: 464 1477 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 505 1692 1941 Size: 505 1187 249 Fragments arranged by size: 1187 505 249 XmaI C'CCGG_G Cuts at: 0 602 1941 Size: 602 1339 Enzymes that do cut: AarI AcuI AflIII AleI AlwNI ApaI ApaLI AvaI BanI BanII BbsI BbvCI BcgI BclI BglI BglII BlpI Bme1580I BmgBI BplI BpmI Bpu10I BpuEI BsaAI BsaHI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspMI BsrBI BsrFI BstAPI BstXI BstYI Bsu36I BtgI CspCI DraIII EaeI EagI EarI EcoICRI Eco57MI EcoNI EcoO109I FalI FspI FspAI HaeII Hin4I HincII MmeI MscI MslI NaeI NcoI NgoMIV NotI NspI PasI PciI PfoI PpiI PpuMI PspOMI PstI SacI SapI SfcI SmaI SmlI SphI SspI StuI StyI XcmI XmaI Enzymes that do not cut: AatII AccI Acc65I AclI AfeI AflII AgeI AhdI AlfI AloI ApoI AscI AseI AsiSI AvrII BaeI BamHI BbeI BciVI BfrBI BmrI BmtI BsaI BsaBI BsaWI BsaXI BsiWI BsmI BspEI BspHI BsrDI BsrGI BssHII BssSI BstBI BstEII BstZ17I BtgZI BtsI ClaI DraI DrdI EciI EcoRI EcoRV FseI HindIII HpaI KasI KpnI MfeI MluI MspA1I NarI NdeI NheI NruI NsiI PacI PflMI PmeI PmlI PshAI PsiI PspXI PsrI PvuI PvuII RsrII SacII SalI SanDI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SnaBI SpeI SrfI SwaI TaqII TatI TstI Tth111I XbaI XhoI XmnI ZraI