Restriction map for
pFN21AB8898
(Linear) (Six-base) MAPSORT of: KIBB8898.seq Check: 4274 from: 1 to: 696
KIBB8898.orf
With 182 enzymes: SgfI *
July 3, 2012 16:36 ..
Acc65I G'GTAC_C
Cuts at: 0 326 696
Size: 326 370
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 532 653 696
Size: 532 121 43
Fragments arranged by size:
532 121 43
AlwNI CAG_nnn'CTG
Cuts at: 0 300 696
Size: 300 396
ApaI G_GGCC'C
Cuts at: 0 66 139 696
Size: 66 73 557
Fragments arranged by size:
557 73 66
ApoI r'AATT_y
Cuts at: 0 606 696
Size: 606 90
AvaI C'yCGr_G
Cuts at: 0 96 178 376 696
Size: 96 82 198 320
Fragments arranged by size:
320 198 96 82
BamHI G'GATC_C
Cuts at: 0 386 696
Size: 386 310
BanI G'GyrC_C
Cuts at: 0 40 326 696
Size: 40 286 370
Fragments arranged by size:
370 286 40
BanII G_rGCy'C
Cuts at: 0 66 103 139 214 308 696
Size: 66 37 36 75 94 388
Fragments arranged by size:
388 94 75 66 37 36
BbeI G_GCGC'C
Cuts at: 0 44 696
Size: 44 652
BbsI GAAGACnn'nnnn_
Cuts at: 0 303 641 696
Size: 303 338 55
Fragments arranged by size:
338 303 55
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1 35 696
Size: 1 34 661
Fragments arranged by size:
661 34 1
BglI GCCn_nnn'nGGC
Cuts at: 0 79 247 696
Size: 79 168 449
Fragments arranged by size:
449 168 79
Bme1580I G_kGCm'C
Cuts at: 0 66 139 696
Size: 66 73 557
Fragments arranged by size:
557 73 66
BmgBI CAC'GTC
Cuts at: 0 272 696
Size: 272 424
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 10 322 696
Size: 10 312 374
Fragments arranged by size:
374 312 10
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 550 696
Size: 550 146
BsaHI Gr'CG_yC
Cuts at: 0 41 250 426 696
Size: 41 209 176 270
Fragments arranged by size:
270 209 176 41
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 577 607 696
Size: 577 30 89
Fragments arranged by size:
577 89 30
BseYI C'CCAG_C
Cuts at: 0 144 518 696
Size: 144 374 178
Fragments arranged by size:
374 178 144
BsiEI CG_ry'CG
Cuts at: 0 171 239 696
Size: 171 68 457
Fragments arranged by size:
457 171 68
BsiHKAI G_wGCw'C
Cuts at: 0 308 489 696
Size: 308 181 207
Fragments arranged by size:
308 207 181
Bsp1286I G_dGCh'C
Cuts at: 0 66 103 139 214 308 489 696
Size: 66 37 36 75 94 181 207
Fragments arranged by size:
207 181 94 75 66 37 36
BsrFI r'CCGG_y
Cuts at: 0 73 183 696
Size: 73 110 513
Fragments arranged by size:
513 110 73
BstYI r'GATC_y
Cuts at: 0 386 696
Size: 386 310
BtgI C'CryG_G
Cuts at: 0 131 696
Size: 131 565
EaeI y'GGCC_r
Cuts at: 0 2 168 236 356 696
Size: 2 166 68 120 340
Fragments arranged by size:
340 166 120 68 2
EagI C'GGCC_G
Cuts at: 0 168 236 696
Size: 168 68 460
Fragments arranged by size:
460 168 68
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 304 696
Size: 304 392
EcoICRI GAG'CTC
Cuts at: 0 306 696
Size: 306 390
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 10 322 532 653 696
Size: 10 312 210 121 43
Fragments arranged by size:
312 210 121 43 10
EcoO109I rG'GnC_Cy
Cuts at: 0 63 136 174 696
Size: 63 73 38 522
Fragments arranged by size:
522 73 63 38
FseI GG_CCGG'CC
Cuts at: 0 187 696
Size: 187 509
HaeII r_GCGC'y
Cuts at: 0 44 85 261 284 354 696
Size: 44 41 176 23 70 342
Fragments arranged by size:
342 176 70 44 41 23
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 563 577 595 609 696
Size: 563 14 18 14 87
Fragments arranged by size:
563 87 18 14 14
KasI G'GCGC_C
Cuts at: 0 40 696
Size: 40 656
KpnI G_GTAC'C
Cuts at: 0 330 696
Size: 330 366
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 471 549 696
Size: 471 78 147
Fragments arranged by size:
471 147 78
MscI TGG'CCA
Cuts at: 0 4 696
Size: 4 692
MspA1I CmG'CkG
Cuts at: 0 133 291 297 522 696
Size: 133 158 6 225 174
Fragments arranged by size:
225 174 158 133 6
NaeI GCC'GGC
Cuts at: 0 75 185 696
Size: 75 110 511
Fragments arranged by size:
511 110 75
NarI GG'CG_CC
Cuts at: 0 41 696
Size: 41 655
NgoMIV G'CCGG_C
Cuts at: 0 73 183 696
Size: 73 110 513
Fragments arranged by size:
513 110 73
NotI GC'GGCC_GC
Cuts at: 0 236 696
Size: 236 460
PflMI CCAn_nnn'nTGG
Cuts at: 0 453 696
Size: 453 243
PfoI T'CCnGG_A
Cuts at: 0 382 489 696
Size: 382 107 207
Fragments arranged by size:
382 207 107
PpuMI rG'GwC_Cy
Cuts at: 0 174 696
Size: 174 522
PspOMI G'GGCC_C
Cuts at: 0 62 135 696
Size: 62 73 561
Fragments arranged by size:
561 73 62
PstI C_TGCA'G
Cuts at: 0 296 696
Size: 296 400
PvuII CAG'CTG
Cuts at: 0 291 297 522 696
Size: 291 6 225 174
Fragments arranged by size:
291 225 174 6
SacI G_AGCT'C
Cuts at: 0 308 696
Size: 308 388
SacII CC_GC'GG
Cuts at: 0 134 696
Size: 134 562
SanDI GG'GwC_CC
Cuts at: 0 174 696
Size: 174 522
SfcI C'TryA_G
Cuts at: 0 292 696
Size: 292 404
SfoI GGC'GCC
Cuts at: 0 42 696
Size: 42 654
SgrAI Cr'CCGG_yG
Cuts at: 0 73 696
Size: 73 623
SmaI CCC'GGG
Cuts at: 0 180 696
Size: 180 516
SmlI C'TyrA_G
Cuts at: 0 529 696
Size: 529 167
StyI C'CwwG_G
Cuts at: 0 597 696
Size: 597 99
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 487 696
Size: 487 209
XmaI C'CCGG_G
Cuts at: 0 178 696
Size: 178 518
XmnI GAAnn'nnTTC
Cuts at: 0 442 696
Size: 442 254
Enzymes that do cut:
Acc65I AcuI AlwNI ApaI ApoI AvaI BamHI BanI
BanII BbeI BbsI BcgI BglI Bme1580I BmgBI BpmI
BpuEI BsaHI BsaXI BseYI BsiEI BsiHKAI Bsp1286I BsrFI
BstYI BtgI EaeI EagI EciI EcoICRI Eco57MI EcoO109I
FseI HaeII Hin4I KasI KpnI MmeI MscI MspA1I
NaeI NarI NgoMIV NotI PflMI PfoI PpuMI PspOMI
PstI PvuII SacI SacII SanDI SfcI SfoI SgrAI
SmaI SmlI StyI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AccI AclI AfeI AflII AflIII AgeI
AhdI AleI AlfI AloI ApaLI AscI AseI AsiSI
AvrII BaeI BbvCI BciVI BclI BfrBI BglII BlpI
BmrI BmtI BplI Bpu10I BsaI BsaAI BsaBI BsaWI
BseRI BsgI BsiWI BsmI BsmBI BspEI BspHI BspMI
BsrBI BsrDI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstZ17I Bsu36I BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EarI EcoNI EcoRI EcoRV FalI FspI
FspAI HincII HindIII HpaI MfeI MluI MslI NcoI
NdeI NheI NruI NsiI NspI PacI PasI PciI
PmeI PmlI PpiI PshAI PsiI PspXI PsrI PvuI
RsrII SalI SapI SbfI ScaI SexAI SfiI SgfI
SnaBI SpeI SphI SrfI SspI StuI SwaI TaqII
TatI TstI Tth111I XbaI XhoI ZraI