################################## # NO CUT ENZYMES ( 205 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcyI AflII AgeI AhdI Alw44I AlwI AlwNI AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AvaI AvaII AviII AvrII BalI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BcnI BfaI BfrI BglI BglII BlnI BlpI BmyI BpmI Bpu1102I BpuAI BsaBI BsaHI BsaI BsaWI BseAI BsgI BsiEI BsiHKAI BsiWI BsmAI BsmBI BsmFI BsoBI Bsp1286I Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrFI BsrGI BsrI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstF5I BstPI BstXI BstYI BstZ17I Bsu36I CelII Cfr10I Cfr13I ClaI CpoI DpnII DraI DraII DraIII DrdI EaeI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRV EcoT22I FbaI FokI FseI FspI HaeII HaeIII HapII HgaI Hin1I HinfI HpaI HpaII HphI KasI KpnI Ksp632I KspI MaeI MamI MboI MfeI MflI MluI MluNI MroI MscI MspA1I MspI MunI NaeI NarI NciI NdeI NdeII NgoMIV NheI NlaIV NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PleI PmeI PpuMI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsrII SacI SacII SalI SapI Sau3AI Sau96I SbfI ScaI SexAI SfaNI SfcI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI TaqI TfiI Tsp45I TspRI Tth111I TthHB8I Van91I XbaI XcmI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 42 enzymes) # ################################### AccII 34 CGCG CG^CG AcsI 190 AAATTC [GA]^AATT[CT] AfaI 9 GTAC GT^AC AflIII 75 ACGTGT A^C[GA][CT]GT ApoI 190 AAATTC [GA]^AATT[CT] BbrPI 74 CACGTG CAC^GTG BbvI 37 GCTGC GCAGC(8/12) BsaAI 74 CACGTG [CT]AC^GT[GA] BseRI 22 GAGGAG GAGGAG(10/8) BsmI 96 GCATTC GAATGC(1/-1) BsrDI 236 GCAATG GCAATG(2/0) BssKI 17 CCTGG ^CC[ACGT]GG BstNI 17 CCTGG CC^[AT]GG BstUI 34 CGCG CG^CG Cac8I 232 GCCCGC GC[ACGT]^[ACGT]GC CfoI 35 GCGC GCG^C DdeI 254 CTGAG C^T[ACGT]AG DsaI 245 CCATGG C^C[GA][CT]GG EcoRII 17 CCTGG ^CC[AT]GG FauI 233 CCCGC CCCGC(4/6) HhaI 35 GCGC GCG^C HinP1I 35 GCGC G^CGC HincII 200 GTCAAC GT[CT]^[GA]AC HindII 200 GTCAAC GT[CT]^[GA]AC HindIII 46 AAGCTT A^AGCTT MaeII 75 ACGT A^CGT MboII 180 GAAGA GAAGA(8/7) MseI 119 TTAA T^TAA MslI 97 CATTCTGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MvaI 17 CCTGG CC^[AT]GG MvnI 34 CGCG CG^CG MwoI 223 GCCAAGGAAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC NcoI 245 CCATGG C^CATGG NlaIII 246 CATG CATG^ PmaCI 74 CACGTG CAC^GTG PmlI 74 CACGTG CAC^GTG RsaI 9 GTAC GT^AC ScrFI 17 CCTGG CC^[ACGT]GG SmlI 167 CTCAAG C^T[CT][GA]AG Tru9I 119 TTAA T^TAA TseI 37 GCTGC G^C[AT]GC Tsp509I 191 AATT ^AATT ################################### # TWO CUT ENZYMES ( 7 enzymes) # ################################### AluI 47 AGCT AG^CT 165 AGCT BsiYI 12 CCAGACCTGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 63 CCCTATGAAGG BslI 12 CCAGACCTGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 63 CCCTATGAAGG EcoT14I 224 CCAAGG C^C[AT][AT]GG 245 CCATGG MaeIII 124 GTAAC ^GT[ACGT]AC 241 GTTAC MnlI 54 CCTC CCTC(7/6) 22 GAGG StyI 224 CCAAGG C^C[AT][AT]GG 245 CCATGG ######################################### # 3 OR MORE CUT ENZYMES ( 4 enzymes) # ######################################### AciI BsaJI Fnu4HI ItaI