################################## # NO CUT ENZYMES ( 120 enzymes) # ################################## AatII AccI AccII AccIII AclI AcyI AflII AflIII AgeI AhdI Alw44I AocI ApaI ApaLI AscI Asp700I AspEI AspI AvaI AviII AvrII BamHI BbeI BbrPI BbsI BbvCI BcgI BciVI BfrI BglII BlnI BpuAI BsaAI BsaBI BsaHI BsaWI BseAI BsiEI BsiWI BsmBI BsoBI Bsp1407I BspDI BspEI BspLU11I BsrGI BssHII BssSI Bst1107I BstAPI BstUI BstZ17I Bsu36I ClaI CpoI DraII DrdI EagI Eam1105I EclXI Eco52I Eco81I EcoNI EcoO109I EcoRV EcoT22I FseI FspI HgaI Hin1I KasI KspI MaeII MamI MluI MroI MvnI NaeI NarI NcoI NdeI NgoMIV NotI NruI NsiI PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PpuMI PshAI Psp1406I PvuI RsrII SacI SacII SalI SbfI ScaI SexAI SfiI SfoI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI StuI SwaI Tth111I XbaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 63 enzymes) # ################################### Acc65I 371 GGTACC G^GTACC AlwNI 1305 CAGCCGCTG CAG[ACGT][ACGT][ACGT]^CTG Aor51HI 122 AGCGCT AGC^GCT AseI 1138 ATTAAT AT^TAAT AsnI 1138 ATTAAT AT^TAAT Asp718I 371 GGTACC G^GTACC AspHI 742 GAGCAC G[AT]GC[AT]^C AvaII 1461 GGACC G^G[AT]CC BalI 1426 TGGCCA TGG^CCA BanII 82 GAGCCC G[GA]GC[CT]^C BclI 1086 TGATCA T^GATCA BcnI 432 CCGGG CC^[GC]GG BlpI 652 GCTGAGC GC^T[ACGT]AGC Bpu1102I 652 GCTGAGC GC^T[ACGT]AGC BseRI 1400 GAGGAG GAGGAG(10/8) BsgI 409 GTGCAG GTGCAG(16/14) BsiHKAI 742 GAGCAC G[AT]GC[AT]^C BspHI 546 TCATGA T^CATGA BspMI 710 ACCTGC ACCTGC(4/8) BsrBI 109 GAGCGG CCGCTC(-3/-3) BstBI 488 TTCGAA TT^CGAA BstEII 1443 GGTGACC G^GT[ACGT]ACC BstPI 1443 GGTGACC G^GT[ACGT]ACC BstXI 65 CCATTTCCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstYI 1665 GGATCT [GA]^GATC[CT] CelII 652 GCTGAGC GC^T[ACGT]AGC CfoI 123 GCGC GCG^C DsaI 1643 CCGTGG C^C[GA][CT]GG Eco47III 122 AGCGCT AGC^GCT EcoO65I 1443 GGTGACC G^GT[ACGT]ACC EcoRI 51 GAATTC G^AATTC FauI 1565 CCCGC CCCGC(4/6) FbaI 1086 TGATCA T^GATCA HaeII 122 AGCGCT [GA]GCGC^[CT] HhaI 123 GCGC GCG^C HinP1I 123 GCGC G^CGC HincII 997 GTTAAC GT[CT]^[GA]AC HindII 997 GTTAAC GT[CT]^[GA]AC HindIII 909 AAGCTT A^AGCTT HpaI 997 GTTAAC GTT^AAC KpnI 371 GGTACC GGTAC^C MflI 1665 GGATCT [GA]^GATC[CT] MluNI 1426 TGGCCA TGG^CCA MscI 1426 TGGCCA TGG^CCA MslI 466 CATTTGAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG NciI 432 CCGGG CC^[GC]GG NheI 840 GCTAGC G^CTAGC NspI 746 ACATGC [GA]CATG^[CT] NspV 488 TTCGAA TT^CGAA PflMI 99 CCAGTTTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PleI 1411 GACTC GAGTC(4/5) PshBI 1138 ATTAAT AT^TAAT PstI 712 CTGCAG CTGCA^G PvuII 1156 CAGCTG CAG^CTG RcaI 546 TCATGA T^CATGA SfuI 488 TTCGAA TT^CGAA SmlI 971 CTCAAG C^T[CT][GA]AG SspI 1141 AATATT AAT^ATT TaqI 489 TCGA T^CGA TfiI 543 GAATC G^A[AT]TC TthHB8I 489 TCGA T^CGA Van91I 99 CCAGTTTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XhoII 1665 GGATCT [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 24 enzymes) # ################################### AcsI 51 GAATTC [GA]^AATT[CT] 1015 AAATTT AlwI 378 GGATC GGATC(4/5) 1665 GGATC ApoI 51 GAATTC [GA]^AATT[CT] 1015 AAATTT BanI 182 GGCACC G^G[CT][GA]CC 371 GGTACC BglI 1307 GCCGCTGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 1428 GCCATTCAGGC BpmI 1189 CTGGAG CTGGAG(16/14) 1660 CTGGAG BsaI 1026 GAGACC GGTCTC(1/5) 1489 GAGACC BsmAI 1026 GAGAC GTCTC(1/5) 1489 GAGAC BsmI 1593 GAATGC GAATGC(1/-1) 726 GCATTC BsrDI 1548 CATTGC GCAATG(2/0) 332 GCAATG BsrFI 368 GCCGGT [GA]^CCGG[CT] 1319 ACCGGC Cac8I 194 GCCAGC GC[ACGT]^[ACGT]GC 840 GCTAGC Cfr10I 368 GCCGGT [GA]^CCGG[CT] 1319 ACCGGC Cfr13I 366 GGGCC G^G[ACGT]CC 1461 GGACC DraIII 145 CACTCGGTG CAC[ACGT][ACGT][ACGT]^GTG 1270 CACACAGTG EaeI 1426 TGGCCA [CT]^GGCC[GA] 1640 TGGCCG EcoT14I 71 CCTTGG C^C[AT][AT]GG 353 CCAAGG HinfI 543 GAATC G^A[ACGT]TC 1411 GACTC MfeI 288 CAATTG C^AATTG 591 CAATTG MspA1I 1156 CAGCTG C[AC]G^C[GT]G 1308 CCGCTG MunI 288 CAATTG C^AATTG 591 CAATTG Sau96I 366 GGGCC G^G[ACGT]CC 1461 GGACC StyI 71 CCTTGG C^C[AT][AT]GG 353 CCAAGG XcmI 168 CCAGACAGGATGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 353 CCAAGGTTATTCTGG ######################################### # 3 OR MORE CUT ENZYMES ( 51 enzymes) # ######################################### AciI AfaI AluI BbvI BfaI BmyI BsaJI BsiYI BslI BsmFI Bsp1286I BsrI BssKI BstF5I BstNI DdeI DpnII DraI EarI EcoRII Fnu4HI FokI HaeIII HapII HpaII HphI ItaI Ksp632I MaeI MaeIII MboI MboII MnlI MseI MspI MvaI MwoI NdeII NlaIII NlaIV RsaI SapI Sau3AI ScrFI SfaNI SfcI Tru9I TseI Tsp45I Tsp509I TspRI