(Circular) (Six-base) MAPSORT of: pFN21AE1979.seq Check: 9843 from: 1 to: 6376 pFN21AE1979 6376 bp With 182 enzymes: SgfI * August 16, 2010 14:44 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4186 278 Size: 53 83 186 936 2650 2468 Fragments arranged by size: 2650 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3719 1937 Size: 1782 4594 Acc65I G'GTAC_C Cuts at: 2376 3697 2376 Size: 1321 5055 AclI AA'CG_TT Cuts at: 1563 4503 4876 1563 Size: 2940 373 3063 Fragments arranged by size: 3063 2940 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2375 2673 3274 4439 5823 6091 1632 Size: 743 298 601 1165 1384 268 1917 Fragments arranged by size: 1917 1384 1165 743 601 298 268 AfeI AGC'GCT Cuts at: 2129 2129 Size: 6376 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6179 AflIII A'CryG_T Cuts at: 1203 5275 6252 1203 Size: 4072 977 1327 Fragments arranged by size: 4072 1327 977 AgeI A'CCGG_T Cuts at: 5207 5207 Size: 6376 AhdI GACnn_n'nnGTC Cuts at: 5105 5105 Size: 6376 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6376 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2493 1184 Size: 1309 5067 AlwNI CAG_nnn'CTG Cuts at: 3315 4119 5691 3315 Size: 804 1572 4000 Fragments arranged by size: 4000 1572 804 ApaLI G'TGCA_C Cuts at: 4433 5589 4433 Size: 1156 5220 ApoI r'AATT_y Cuts at: 1493 2056 3020 3685 3911 4063 6172 1493 Size: 563 964 665 226 152 2109 1697 Fragments arranged by size: 2109 1697 964 665 563 226 152 AseI AT'TA_AT Cuts at: 160 1032 3144 4930 160 Size: 872 2112 1786 1606 Fragments arranged by size: 2112 1786 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6376 AvaI C'yCGr_G Cuts at: 1870 1943 3701 1870 Size: 73 1758 4545 Fragments arranged by size: 4545 1758 73 BamHI G'GATC_C Cuts at: 3706 4073 3706 Size: 367 6009 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2187 2376 3697 5152 618 Size: 325 217 627 400 189 1321 1455 1842 Fragments arranged by size: 1842 1455 1321 627 400 325 217 189 BanII G_rGCy'C Cuts at: 1391 2091 3695 1391 Size: 700 1604 4072 Fragments arranged by size: 4072 1604 700 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5405 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4567 4601 1903 Size: 34 2630 34 3678 Fragments arranged by size: 3678 2630 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4268 5478 4268 Size: 1210 5166 BclI T'GATC_A Cuts at: 1548 3091 1548 Size: 1543 4833 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3318 3439 4987 Size: 107 122 71 631 605 1646 121 1548 Cuts at: 4987 136 Size: 1525 Fragments arranged by size: 1646 1548 1525 631 605 122 121 107 71 BglII A'GATC_T Cuts at: 6371 6371 Size: 6376 BlpI GC'TnA_GC Cuts at: 2658 3793 2658 Size: 1135 5241 Bme1580I G_kGCm'C Cuts at: 1790 2190 3276 4437 5593 1790 Size: 400 1086 1161 1156 2573 Fragments arranged by size: 2573 1161 1156 1086 400 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6376 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2085 2573 4107 Size: 416 53 83 399 24 668 488 1534 Cuts at: 4107 5065 442 Size: 958 1753 Fragments arranged by size: 1753 1534 958 668 488 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2849 1056 Size: 1793 4583 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2330 3433 2330 Size: 1103 5273 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3215 3686 5036 1239 Size: 1976 471 1350 2579 Fragments arranged by size: 2579 1976 1350 471 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2961 4501 5366 5664 2961 Size: 1540 865 298 3673 Fragments arranged by size: 3673 1540 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3025 3488 5039 882 Size: 617 1526 463 1551 2219 Fragments arranged by size: 2219 1551 1526 617 463 BsaAI yAC'GTr Cuts at: 493 1204 4167 493 Size: 711 2963 2702 Fragments arranged by size: 2963 2702 711 BsaBI GATnn'nnATC Cuts at: 6370 6370 Size: 6376 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4183 4565 5200 275 Size: 53 83 186 936 2650 382 635 1451 Fragments arranged by size: 2650 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4809 5207 5481 5628 6039 4809 Size: 398 274 147 411 5146 Fragments arranged by size: 5146 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6151 Fragments arranged by size: 6151 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3420 1186 Size: 2234 4142 BseYI C'CCAG_C Cuts at: 5579 6075 5579 Size: 496 5880 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2435 1163 Size: 573 699 5104 Fragments arranged by size: 5104 699 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3826 4587 5615 1540 Size: 275 186 1825 761 1028 2301 Fragments arranged by size: 2301 1825 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2751 4437 4522 5593 2751 Size: 1686 85 1071 3534 Fragments arranged by size: 3534 1686 1071 85 BsmI GAATG_Cn' Cuts at: 2731 3604 3887 3980 2731 Size: 873 283 93 5127 Fragments arranged by size: 5127 873 283 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2091 2190 2751 3276 3695 4437 4522 Size: 399 301 99 561 525 419 742 85 Cuts at: 4522 5593 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 561 525 419 399 301 99 85 BspEI T'CCGG_A Cuts at: 6039 6039 Size: 6376 BspHI T'CATG_A Cuts at: 2551 4265 5995 2551 Size: 1714 1730 2932 Fragments arranged by size: 2932 1730 1714 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2724 3731 844 Size: 1880 1007 3489 Fragments arranged by size: 3489 1880 1007 BsrBI CCG'CTC Cuts at: 1394 1823 2116 4263 1394 Size: 429 293 2147 3507 Fragments arranged by size: 3507 2147 429 293 BsrDI GCAATG_nn' Cuts at: 65 1248 2344 3552 4871 5045 65 Size: 1183 1096 1208 1319 174 1396 Fragments arranged by size: 1396 1319 1208 1183 1096 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2373 3324 5020 5207 1700 Size: 110 563 951 1696 187 2869 Fragments arranged by size: 2869 1696 951 563 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6376 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6376 BssSI C'ACGA_G Cuts at: 4436 5448 4436 Size: 1012 5364 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6376 BstBI TT'CG_AA Cuts at: 2494 5180 2494 Size: 2686 3690 BstEII G'GTnAC_C Cuts at: 1184 3448 1184 Size: 2264 4112 BstXI CCAn_nnnn'nTGG Cuts at: 2077 2077 Size: 6376 BstYI r'GATC_y Cuts at: 1972 3670 3706 4073 4458 4475 5927 6013 6025 Size: 1698 36 367 385 17 1452 86 12 Cuts at: 6025 6371 1972 Size: 346 1977 Fragments arranged by size: 1977 1698 1452 385 367 346 86 36 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 3648 513 Size: 552 939 1644 3241 Fragments arranged by size: 3241 1644 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5637 Fragments arranged by size: 5637 683 56 BtsI GCAGTG_nn' Cuts at: 2203 2402 2423 3434 3892 4686 4706 2203 Size: 199 21 1011 458 794 20 3873 Fragments arranged by size: 3873 1011 794 458 199 21 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6376 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6341 DraI TTT'AAA Cuts at: 2287 3187 3529 3680 2287 Size: 900 342 151 4983 Fragments arranged by size: 4983 900 342 151 DraIII CAC_nnn'GTG Cuts at: 2155 3280 2155 Size: 1125 5251 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5383 809 Size: 507 4067 1802 Fragments arranged by size: 4067 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3431 3645 3823 Size: 54 1425 50 275 18 1601 214 178 Cuts at: 3823 4712 6341 8 Size: 889 1629 43 Fragments arranged by size: 1629 1601 1425 889 275 214 178 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3823 1537 Size: 275 2011 4090 Fragments arranged by size: 4090 2011 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2013 2118 3108 3171 3248 4306 1353 Size: 660 105 990 63 77 1058 3423 Fragments arranged by size: 3423 1058 990 660 105 77 63 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2039 4963 5337 5483 387 Size: 1064 138 450 2924 374 146 1280 Fragments arranged by size: 2924 1280 1064 450 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2375 2673 3215 3274 3686 4439 5036 Size: 393 743 298 542 59 412 753 597 Cuts at: 5036 5823 6091 1239 Size: 787 268 1524 Fragments arranged by size: 1524 787 753 743 597 542 412 393 298 268 59 EcoRI G'AATT_C Cuts at: 2056 3685 4063 2056 Size: 1629 378 4369 Fragments arranged by size: 4369 1629 378 FspI TGC'GCA Cuts at: 4882 4882 Size: 6376 HaeII r_GCGC'y Cuts at: 1678 2131 5523 1678 Size: 453 3392 2531 Fragments arranged by size: 3392 2531 453 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 5017 5049 5091 5123 1373 Size: 32 135 32 3445 32 42 32 2626 Fragments arranged by size: 3445 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3004 3720 3966 669 Size: 988 281 1066 716 246 3079 Fragments arranged by size: 3079 1066 988 716 281 246 HindIII A'AGCT_T Cuts at: 748 2914 748 Size: 2166 4210 HpaI GTT'AAC Cuts at: 3004 3966 3004 Size: 962 5414 KpnI G_GTAC'C Cuts at: 2380 3701 2380 Size: 1321 5055 MfeI C'AATT_G Cuts at: 2293 2596 3975 2293 Size: 303 1379 4694 Fragments arranged by size: 4694 1379 303 MluI A'CGCG_T Cuts at: 6252 6252 Size: 6376 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2100 5490 5674 1305 Size: 795 3390 184 2007 Fragments arranged by size: 3390 2007 795 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3433 10 Size: 54 1425 343 1601 2953 Fragments arranged by size: 2953 1601 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2475 4334 4693 4852 518 Size: 648 1309 1859 359 159 2042 Fragments arranged by size: 2042 1859 1309 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3163 3315 3792 4469 5617 5862 1606 Size: 1557 152 477 677 1148 245 2120 Fragments arranged by size: 2120 1557 1148 677 477 245 152 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4885 Fragments arranged by size: 4885 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6376 NheI G'CTAG_C Cuts at: 1052 2845 1052 Size: 1793 4583 NotI GC'GGCC_GC Cuts at: 3823 3823 Size: 6376 NspI r_CATG'y Cuts at: 1132 1229 2755 3734 5279 1132 Size: 97 1526 979 1545 2229 Fragments arranged by size: 2229 1545 1526 979 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6376 PciI A'CATG_T Cuts at: 5275 5275 Size: 6376 PflMI CCAn_nnn'nTGG Cuts at: 1108 2110 1108 Size: 1002 5374 PfoI T'CCnGG_A Cuts at: 2344 2344 Size: 6376 PmeI GTTT'AAAC Cuts at: 3680 3680 Size: 6376 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4445 4477 5958 5990 4445 Size: 32 1481 32 4831 Fragments arranged by size: 4831 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6376 PsiI TTA'TAA Cuts at: 3946 3946 Size: 6376 PstI C_TGCA'G Cuts at: 830 2721 3728 830 Size: 1891 1007 3478 Fragments arranged by size: 3478 1891 1007 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6376 PvuII CAG'CTG Cuts at: 3163 3163 Size: 6376 SalI G'TCGA_C Cuts at: 1936 3718 1936 Size: 1782 4594 SapI GCTCTTCn'nnn_ Cuts at: 2013 2118 3171 2013 Size: 105 1053 5218 Fragments arranged by size: 5218 1053 105 SbfI CC_TGCA'GG Cuts at: 3728 3728 Size: 6376 ScaI AGT'ACT Cuts at: 4624 6336 4624 Size: 1712 4664 SfcI C'TryA_G Cuts at: 826 1045 2250 2543 2717 3724 4859 5540 5731 Size: 219 1205 293 174 1007 1135 681 191 Cuts at: 5731 826 Size: 1471 Fragments arranged by size: 1471 1205 1135 1007 681 293 219 191 174 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6376 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6376 SmaI CCC'GGG Cuts at: 1872 3703 1872 Size: 1831 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2976 4480 5381 5643 820 Size: 197 926 1033 1504 901 262 1553 Fragments arranged by size: 1553 1504 1033 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4167 493 Size: 3674 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6376 SphI G_CATG'C Cuts at: 1132 3734 1132 Size: 2602 3774 SspI AAT'ATT Cuts at: 5 52 3148 4300 5 Size: 47 3096 1152 2081 Fragments arranged by size: 3096 2081 1152 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6376 StyI C'CwwG_G Cuts at: 513 1065 2004 2076 2358 3815 513 Size: 552 939 72 282 1457 3074 Fragments arranged by size: 3074 1457 939 552 282 72 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4403 4420 4573 4758 5203 325 Size: 1255 2823 17 153 185 445 1498 Fragments arranged by size: 2823 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4622 6334 96 Size: 274 80 33 51 1443 2645 1712 138 Fragments arranged by size: 2645 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2549 2581 3266 3298 6336 6368 2549 Size: 32 685 32 3038 32 2557 Fragments arranged by size: 3038 2557 685 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6376 XbaI T'CTAG_A Cuts at: 3712 3712 Size: 6376 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2180 2365 2180 Size: 185 6191 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6376 XmaI C'CCGG_G Cuts at: 1870 3701 1870 Size: 1831 4545 XmnI GAAnn'nnTTC Cuts at: 4505 4505 Size: 6376 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4184 276 Size: 53 83 186 936 2650 2468 Fragments arranged by size: 2650 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI PvuII SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI Bpu10I BsiWI BsmBI BstZ17I Bsu36I EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI