################################## # NO CUT ENZYMES ( 172 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcsI AcyI AflII AflIII AgeI AhdI Aor51HI ApaI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvaI AviII AvrII BalI BanII BbeI BbrPI BbvCI BciVI BclI BcnI BfrI BglII BlnI BlpI Bpu1102I BsaAI BsaBI BsaHI BsaI BsaWI BseAI BsiEI BsiWI BsmAI BsmBI BsmI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrFI BsrGI BssHII BssSI Bst1107I BstBI BstEII BstPI BstUI BstXI BstZ17I CelII CfoI Cfr10I ClaI CpoI DraI DraIII DrdI EaeI EagI Eam1105I EarI EclXI Eco47III Eco52I EcoNI EcoO65I EcoRI EcoRV EcoT22I FauI FbaI FseI FspI HaeII HapII HgaI HhaI Hin1I HinP1I HincII HindII HindIII HpaI HpaII KasI KpnI Ksp632I KspI MamI MfeI MluI MluNI MroI MscI MspA1I MspI MunI MvnI NaeI NarI NciI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PinAI PleI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsrII SacI SacII SalI SapI SbfI ScaI SfcI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI StuI SwaI TaqI Tth111I TthHB8I XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 38 enzymes) # ################################### AciI 228 CCGC CCGC(-3/-1) AfaI 504 GTAC GT^AC Alw44I 557 GTGCAC G^TGCAC AocI 467 CCTTAGG CC^T[ACGT]AGG ApaLI 557 GTGCAC G^TGCAC AvaII 369 GGACC G^G[AT]CC BamHI 472 GGATCC G^GATCC BanI 381 GGCACC G^G[CT][GA]CC BcgI 95 GCATATTTGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BfaI 364 CTAG C^TAG BglI 227 GCCGCTTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BseRI 700 GAGGAG GAGGAG(10/8) BsgI 520 GTGCAG GTGCAG(16/14) BstAPI 613 GCACTTCTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstF5I 333 CATCC GGATG(2/0) BstYI 472 GGATCC [GA]^GATC[CT] Bsu36I 467 CCTTAGG CC^T[ACGT]AGG DraII 282 AGGGCCT [GA]G^G[ACGT]CC[CT] DsaI 653 CCATGG C^C[GA][CT]GG Eco81I 467 CCTTAGG CC^T[ACGT]AGG EcoO109I 282 AGGGCCT [GA]G^G[ACGT]CC[CT] EcoT14I 653 CCATGG C^C[AT][AT]GG FokI 333 CATCC GGATG(9/13) MaeI 364 CTAG C^TAG MflI 472 GGATCC [GA]^GATC[CT] MseI 676 TTAA T^TAA NcoI 653 CCATGG C^CATGG PflMI 433 CCAGTTCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG RsaI 504 GTAC GT^AC SexAI 161 ACCTGGT A^CC[AT]GGT SfaNI 55 GATGC GCATC(5/9) SmlI 337 CTCAAG C^T[CT][GA]AG SspI 710 AATATT AAT^ATT StyI 653 CCATGG C^C[AT][AT]GG Tru9I 676 TTAA T^TAA Van91I 433 CCAGTTCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XbaI 363 TCTAGA T^CTAGA XhoII 472 GGATCC [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 21 enzymes) # ################################### AlwNI 124 CAGATTCTG CAG[ACGT][ACGT][ACGT]^CTG 281 CAGGGCCTG AspHI 61 GAGCAC G[AT]GC[AT]^C 557 GTGCAC BbsI 174 GAAGAC GAAGAC(2/6) 496 GAAGAC BbvI 344 GCAGC GCAGC(8/12) 528 GCAGC BpmI 80 CTCCAG CTGGAG(16/14) 718 CTGGAG BpuAI 174 GAAGAC GAAGAC(2/6) 496 GAAGAC BsiHKAI 61 GAGCAC G[AT]GC[AT]^C 557 GTGCAC BsiYI 372 CCACACCTGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 433 CCAGTTCATGG BslI 372 CCACACCTGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 433 CCAGTTCATGG BsmFI 133 GGGAC GGGAC(10/14) 410 GGGAC Cac8I 16 GCGTGC GC[ACGT]^[ACGT]GC 518 GCGTGC HaeIII 235 GGCC GG^CC 284 GGCC HinfI 126 GATTC G^A[ACGT]TC 429 GAATC HphI 258 GGTGA GGTGA(8/7) 464 TCACC MaeII 140 ACGT A^CGT 310 ACGT MaeIII 299 GTGAC ^GT[ACGT]AC 416 GTGAC MslI 309 CACGTTTATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 690 CATGACAATG TfiI 126 GATTC G^A[AT]TC 429 GAATC TseI 344 GCAGC G^C[AT]GC 528 GCAGC Tsp45I 299 GTGAC ^GT[GC]AC 416 GTGAC Tsp509I 674 AATT ^AATT 749 AATT ######################################### # 3 OR MORE CUT ENZYMES ( 27 enzymes) # ######################################### AluI AlwI BmyI BsaJI Bsp1286I BsrDI BsrI BssKI BstNI Cfr13I DdeI DpnII EcoRII Fnu4HI ItaI MboI MboII MnlI MvaI MwoI NdeII NlaIII NlaIV Sau3AI Sau96I ScrFI TspRI