################################## # NO CUT ENZYMES ( 96 enzymes) # ################################## AatII AccIII AclI AflII AflIII AgeI AhdI Alw44I ApaLI AscI AseI AsnI Asp700I AspEI AviII AvrII BbeI BbvCI BciVI BfrI BglII BlnI BsaBI BsaWI BseAI BsgI BspDI BspEI BspHI BspLU11I BsrBI BssHII BssSI Bst1107I BstBI BstZ17I ClaI CpoI DraI DraIII EagI Eam1105I EclXI Eco52I EcoRI EcoRV EcoT22I FseI FspI HindIII HpaI KasI KspI MamI MfeI MluI MroI MunI NarI NcoI NdeI NheI NotI NruI NsiI NspV PacI PaeR7I PflMI PinAI PmeI PshAI PshBI Psp1406I PvuI RcaI RsrII SacI SacII SbfI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspI SwaI Van91I XbaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 39 enzymes) # ################################### Acc65I 1395 GGTACC G^GTACC AccII 1711 CGCG CG^CG AcyI 1303 GACGCC G[GA]^CG[CT]C Aor51HI 1914 AGCGCT AGC^GCT Asp718I 1395 GGTACC G^GTACC AspI 838 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC BbrPI 114 CACGTG CAC^GTG BcgI 1541 GCAAGGCCTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BclI 932 TGATCA T^GATCA BsaAI 114 CACGTG [CT]AC^GT[GA] BsaHI 1303 GACGCC G[GA]^CG[CT]C BsiEI 2007 CGACCG CG[GA][CT]^CG BsiWI 1469 CGTACG C^GTACG BsmBI 219 CGTCTC CGTCTC(1/5) Bsp1407I 494 TGTACA T^GTACA BspMI 19 ACCTGC ACCTGC(4/8) BsrDI 288 CATTGC GCAATG(2/0) BsrGI 494 TGTACA T^GTACA BstAPI 1732 GCAGACGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstUI 1711 CGCG CG^CG Eco47III 1914 AGCGCT AGC^GCT EcoT14I 2204 CCAAGG C^C[AT][AT]GG FbaI 932 TGATCA T^GATCA Hin1I 1303 GACGCC G[GA]^CG[CT]C KpnI 1395 GGTACC GGTAC^C MseI 1214 TTAA T^TAA MvnI 1711 CGCG CG^CG NspI 2045 GCATGT [GA]CATG^[CT] PflFI 838 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC PmaCI 114 CACGTG CAC^GTG PmlI 114 CACGTG CAC^GTG SalI 2005 GTCGAC G^TCGAC SapI 6 GCTCTTC GCTCTTC(1/4) SexAI 1595 ACCTGGT A^CC[AT]GGT SfiI 2259 GGCCGCCGAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SspBI 494 TGTACA T^GTACA StyI 2204 CCAAGG C^C[AT][AT]GG Tru9I 1214 TTAA T^TAA Tth111I 838 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC ################################### # TWO CUT ENZYMES ( 23 enzymes) # ################################### AccI 415 GTCTAC GT^[AC][GT]AC 2005 GTCGAC BamHI 887 GGATCC G^GATCC 1800 GGATCC BbsI 739 GAAGAC GAAGAC(2/6) 34 GTCTTC BglI 2215 GCCGAGATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 2260 GCCGCCGAGGC BlpI 297 GCTGAGC GC^T[ACGT]AGC 633 GCTCAGC Bpu1102I 297 GCTGAGC GC^T[ACGT]AGC 633 GCTCAGC BpuAI 739 GAAGAC GAAGAC(2/6) 34 GTCTTC BsaI 2170 GAGACC GGTCTC(1/5) 1920 GGTCTC BsmI 471 GAATGC GAATGC(1/-1) 693 GAATGC BstEII 441 GGTGACC G^GT[ACGT]ACC 1599 GGTGACC BstPI 441 GGTGACC G^GT[ACGT]ACC 1599 GGTGACC BstYI 887 GGATCC [GA]^GATC[CT] 1800 GGATCC CelII 297 GCTGAGC GC^T[ACGT]AGC 633 GCTCAGC DrdI 406 GACACTAAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 838 GACGTGGTCGTC EcoO65I 441 GGTGACC G^GT[ACGT]ACC 1599 GGTGACC HgaI 1303 GACGC GACGC(5/10) 1735 GACGC MflI 887 GGATCC [GA]^GATC[CT] 1800 GGATCC PstI 997 CTGCAG CTGCA^G 1664 CTGCAG PvuII 295 CAGCTG CAG^CTG 994 CAGCTG ScaI 158 AGTACT AGT^ACT 1145 AGTACT SmlI 1390 CTCAAG C^T[CT][GA]AG 1432 CTCAAG XcmI 903 CCAGGACAAGGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 2081 CCAATGACAACATGG XhoII 887 GGATCC [GA]^GATC[CT] 1800 GGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 100 enzymes) # ######################################### AciI AcsI AfaI AluI AlwI AlwNI AocI ApaI ApoI AspHI AvaI AvaII BalI BanI BanII BbvI BcnI BfaI BmyI BpmI BsaJI BseRI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BsrFI BsrI BssKI BstF5I BstNI BstXI Bsu36I Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII DsaI EaeI EarI Eco81I EcoNI EcoO109I EcoRII FauI Fnu4HI FokI HaeII HaeIII HapII HhaI HinP1I HincII HindII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MluNI MnlI MscI MslI MspA1I MspI MvaI MwoI NaeI NciI NdeII NgoMIV NlaIII NlaIV PleI PpuMI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI StuI TaqI TfiI TseI Tsp45I Tsp509I TspRI TthHB8I