(Circular) (Six-base) MAPSORT of: pFN21AE2089.seq Check: 7213 from: 1 to: 6997 pFN21AE2089 6997 bp With 182 enzymes: SgfI * August 16, 2010 14:53 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2033 2033 Size: 6997 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4807 278 Size: 53 83 186 936 3271 2468 Fragments arranged by size: 3271 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2421 4011 4340 1937 Size: 484 1590 329 4594 Fragments arranged by size: 4594 1590 484 329 Acc65I G'GTAC_C Cuts at: 3400 4318 3400 Size: 918 6079 AclI AA'CG_TT Cuts at: 1563 5124 5497 1563 Size: 3561 373 3063 Fragments arranged by size: 3561 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 5060 6444 6712 1632 Size: 3428 1384 268 1917 Fragments arranged by size: 3428 1917 1384 268 AfeI AGC'GCT Cuts at: 3921 3921 Size: 6997 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6800 AflIII A'CryG_T Cuts at: 1203 5896 6873 1203 Size: 4693 977 1327 Fragments arranged by size: 4693 1327 977 AgeI A'CCGG_T Cuts at: 5828 5828 Size: 6997 AhdI GACnn_n'nnGTC Cuts at: 5726 5726 Size: 6997 AleI CACnn'nnGTG Cuts at: 1166 2282 3077 3419 3935 1166 Size: 1116 795 342 516 4228 Fragments arranged by size: 4228 1116 795 516 342 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2912 1184 Size: 1728 5269 AlwNI CAG_nnn'CTG Cuts at: 2608 3520 3743 4740 6312 2608 Size: 912 223 997 1572 3293 Fragments arranged by size: 3293 1572 997 912 223 ApaI G_GGCC'C Cuts at: 2786 2875 3175 2786 Size: 89 300 6608 Fragments arranged by size: 6608 300 89 ApaLI G'TGCA_C Cuts at: 5054 6210 5054 Size: 1156 5841 ApoI r'AATT_y Cuts at: 1493 2807 3464 4076 4306 4532 4684 6793 1493 Size: 1314 657 612 230 226 152 2109 1697 Fragments arranged by size: 2109 1697 1314 657 612 230 226 152 AseI AT'TA_AT Cuts at: 160 1032 5551 160 Size: 872 4519 1606 Fragments arranged by size: 4519 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6997 AvaI C'yCGr_G Cuts at: 1870 1943 2078 2723 2729 4322 1870 Size: 73 135 645 6 1593 4545 Fragments arranged by size: 4545 1593 645 135 73 6 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 3619 3652 3619 Size: 33 6964 BamHI G'GATC_C Cuts at: 2892 3805 4327 4694 2892 Size: 913 522 367 5195 Fragments arranged by size: 5195 913 522 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2169 3269 3400 3937 4318 Size: 325 217 627 382 1100 131 537 381 Cuts at: 4318 5773 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 1100 627 537 382 381 325 217 131 BanII G_rGCy'C Cuts at: 1391 2403 2730 2759 2786 2875 2986 3175 3994 Size: 1012 327 29 27 89 111 189 819 Cuts at: 3994 4316 1391 Size: 322 4072 Fragments arranged by size: 4072 1012 819 327 322 189 111 89 29 27 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2032 2751 928 Size: 971 133 719 5174 Fragments arranged by size: 5174 971 719 133 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 3535 3569 5188 5222 1903 Size: 34 50 34 1514 34 1619 34 3678 Fragments arranged by size: 3678 1619 1514 50 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4889 6099 4889 Size: 1210 5787 BclI T'GATC_A Cuts at: 1548 2937 1548 Size: 1389 5608 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4226 4271 5608 Size: 107 122 71 631 605 2554 45 1337 Cuts at: 5608 136 Size: 1525 Fragments arranged by size: 2554 1525 1337 631 605 122 107 71 45 BglII A'GATC_T Cuts at: 6992 6992 Size: 6997 BlpI GC'TnA_GC Cuts at: 2303 2639 4414 2303 Size: 336 1775 4886 Fragments arranged by size: 4886 1775 336 Bme1580I G_kGCm'C Cuts at: 1790 2779 2786 2875 3175 3942 5058 6214 1790 Size: 989 7 89 300 767 1116 1156 2573 Fragments arranged by size: 2573 1156 1116 989 767 300 89 7 BmgBI CAC'GTC Cuts at: 1318 2845 1318 Size: 1527 5470 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3220 4728 5686 Size: 416 53 83 399 24 1803 1508 958 Cuts at: 5686 442 Size: 1753 Fragments arranged by size: 1803 1753 1508 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6997 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3452 3452 Size: 6997 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2212 2399 2524 3272 3370 3898 4054 4232 Size: 973 187 125 748 98 528 156 178 Cuts at: 4232 5657 1239 Size: 1425 2579 Fragments arranged by size: 2579 1425 973 748 528 187 178 156 125 98 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3380 3422 5122 5987 6285 3380 Size: 42 1700 865 298 4092 Fragments arranged by size: 4092 1700 865 298 42 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3931 4169 5660 882 Size: 617 2432 238 1491 2219 Fragments arranged by size: 2432 2219 1491 617 238 BsaAI yAC'GTr Cuts at: 493 1204 2121 4788 493 Size: 711 917 2667 2702 Fragments arranged by size: 2702 2667 917 711 BsaBI GATnn'nnATC Cuts at: 6991 6991 Size: 6997 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3309 4804 5186 5821 Size: 53 83 186 936 1776 1495 382 635 Cuts at: 5821 275 Size: 1451 Fragments arranged by size: 1776 1495 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5430 5828 6102 6249 6660 5430 Size: 398 274 147 411 5767 Fragments arranged by size: 5767 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3087 3117 1178 Size: 30 165 30 1684 30 5058 Fragments arranged by size: 5058 1684 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2096 2200 3062 3269 3460 1186 Size: 910 104 862 207 191 4723 Fragments arranged by size: 4723 910 862 207 191 104 BseYI C'CCAG_C Cuts at: 2044 2298 2648 2669 2875 3424 3524 3542 6200 Size: 254 350 21 206 549 100 18 2658 Cuts at: 6200 6696 2044 Size: 496 2345 Fragments arranged by size: 2658 2345 549 496 350 254 206 100 21 18 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 6424 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4015 4447 5208 6236 1540 Size: 275 186 2014 432 761 1028 2301 Fragments arranged by size: 2301 2014 1028 761 432 275 186 BsiHKAI G_wGCw'C Cuts at: 2022 3114 4169 5058 5143 6214 2022 Size: 1092 1055 889 85 1071 2805 Fragments arranged by size: 2805 1092 1071 1055 889 85 BsiWI C'GTAC_G Cuts at: 3474 3474 Size: 6997 BsmI GAATG_Cn' Cuts at: 2482 2704 4508 4601 2482 Size: 222 1804 93 4878 Fragments arranged by size: 4878 1804 222 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2230 2230 Size: 6997 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2022 2403 2730 2759 2779 2786 2875 Size: 399 232 381 327 29 20 7 89 Cuts at: 2875 2986 3114 3175 3942 3994 4169 4316 5058 Size: 111 128 61 767 52 175 147 742 Cuts at: 5058 5143 6214 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 767 742 399 381 327 232 175 147 128 111 89 85 61 52 29 20 7 BspEI T'CCGG_A Cuts at: 6660 6660 Size: 6997 BspHI T'CATG_A Cuts at: 4886 6616 4886 Size: 1730 5267 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2033 4352 844 Size: 1189 2319 3489 Fragments arranged by size: 3489 2319 1189 BsrBI CCG'CTC Cuts at: 1394 1823 4884 1394 Size: 429 3061 3507 Fragments arranged by size: 3507 3061 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2292 5492 5666 65 Size: 1183 1044 3200 174 1396 Fragments arranged by size: 3200 1396 1183 1044 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2148 2172 3311 3576 5641 5828 1700 Size: 110 338 24 1139 265 2065 187 2869 Fragments arranged by size: 2869 2065 1139 338 265 187 110 24 BsrGI T'GTAC_A Cuts at: 96 2499 96 Size: 2403 4594 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6997 BssSI C'ACGA_G Cuts at: 5057 6069 5057 Size: 1012 5985 BstAPI GCAn_nnn'nTGC Cuts at: 794 3743 794 Size: 2949 4048 BstBI TT'CG_AA Cuts at: 5801 5801 Size: 6997 BstEII G'GTnAC_C Cuts at: 1184 2446 3604 1184 Size: 1262 1158 4577 Fragments arranged by size: 4577 1262 1158 BstXI CCAn_nnnn'nTGG Cuts at: 2396 2993 3659 3922 4261 2396 Size: 597 666 263 339 5132 Fragments arranged by size: 5132 666 597 339 263 BstYI r'GATC_y Cuts at: 1972 2892 3805 4327 4694 5079 5096 6548 6634 Size: 920 913 522 367 385 17 1452 86 Cuts at: 6634 6646 6992 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 920 913 522 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2750 2787 2948 2750 Size: 37 161 6799 Fragments arranged by size: 6799 161 37 BtgI C'CryG_G Cuts at: 513 1065 3360 3969 4260 513 Size: 552 2295 609 291 3250 Fragments arranged by size: 3250 2295 609 552 291 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2658 3732 487 Size: 683 56 1432 1074 3752 Fragments arranged by size: 3752 1432 1074 683 56 BtsI GCAGTG_nn' Cuts at: 2833 3966 4513 5307 5327 2833 Size: 1133 547 794 20 4503 Fragments arranged by size: 4503 1133 794 547 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6997 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6962 DraI TTT'AAA Cuts at: 4301 4301 Size: 6997 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2417 2849 6004 809 Size: 507 1101 432 3155 1802 Fragments arranged by size: 3155 1802 1101 507 432 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2599 2802 3105 Size: 54 1425 50 275 18 769 203 303 Cuts at: 3105 3181 3984 4263 4444 5333 6962 8 Size: 76 803 279 181 889 1629 43 Fragments arranged by size: 1629 1425 889 803 769 303 279 275 203 181 76 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4444 1537 Size: 275 2632 4090 Fragments arranged by size: 4090 2632 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2018 2054 2861 3041 3687 4111 4137 4927 Size: 665 36 807 180 646 424 26 790 Cuts at: 4927 1353 Size: 3423 Fragments arranged by size: 3423 807 790 665 646 424 180 36 26 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5584 5958 6104 387 Size: 1064 138 3995 374 146 1280 Fragments arranged by size: 3995 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2212 2399 2524 3272 3370 3898 4054 Size: 393 580 187 125 748 98 528 156 Cuts at: 4054 4232 5060 5657 6444 6712 1239 Size: 178 828 597 787 268 1524 Fragments arranged by size: 1524 828 787 748 597 580 528 393 268 187 178 156 125 98 EcoNI CCTnn'n_nnAGG Cuts at: 2951 3251 3731 2951 Size: 300 480 6217 Fragments arranged by size: 6217 480 300 EcoO109I rG'GnC_Cy Cuts at: 2393 2783 2871 2872 3171 3442 3517 3539 3711 Size: 390 88 1 299 271 75 22 172 Cuts at: 3711 2393 Size: 5679 Fragments arranged by size: 5679 390 299 271 172 88 75 22 1 EcoRI G'AATT_C Cuts at: 4306 4684 4306 Size: 378 6619 FspI TGC'GCA Cuts at: 5503 5503 Size: 6997 HaeII r_GCGC'y Cuts at: 1678 2326 2971 3923 6144 1678 Size: 648 645 952 2221 2531 Fragments arranged by size: 2531 2221 952 648 645 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3909 3941 4008 4040 5638 Size: 32 135 32 2337 32 67 32 1598 Cuts at: 5638 5670 5712 5744 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 2337 1598 135 67 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2146 2854 4012 4341 4587 669 Size: 988 281 208 708 1158 329 246 3079 Fragments arranged by size: 3079 1158 988 708 329 281 246 208 HindIII A'AGCT_T Cuts at: 748 748 Size: 6997 HpaI GTT'AAC Cuts at: 4587 4587 Size: 6997 KpnI G_GTAC'C Cuts at: 3404 4322 3404 Size: 918 6079 MfeI C'AATT_G Cuts at: 4596 4596 Size: 6997 MluI A'CGCG_T Cuts at: 6873 6873 Size: 6997 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 6111 6295 1305 Size: 4806 184 2007 Fragments arranged by size: 4806 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2601 2804 3986 10 Size: 54 1425 343 769 203 1182 3021 Fragments arranged by size: 3021 1425 1182 769 343 203 54 MslI CAynn'nnrTG Cuts at: 518 1166 2282 2991 3077 3419 3657 3935 4049 Size: 648 1116 709 86 342 238 278 114 Cuts at: 4049 4955 5314 5473 518 Size: 906 359 159 2042 Fragments arranged by size: 2042 1116 906 709 648 359 342 278 238 159 114 86 MspA1I CmG'CkG Cuts at: 1606 2302 3001 3186 3707 4413 5090 6238 6483 Size: 696 699 185 521 706 677 1148 245 Cuts at: 6483 1606 Size: 2120 Fragments arranged by size: 2120 1148 706 699 696 677 521 245 185 NaeI GCC'GGC Cuts at: 2174 3313 3578 2174 Size: 1139 265 5593 Fragments arranged by size: 5593 1139 265 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 6445 NdeI CA'TA_TG Cuts at: 387 387 Size: 6997 NgoMIV G'CCGG_C Cuts at: 2172 3311 3576 2172 Size: 1139 265 5593 Fragments arranged by size: 5593 1139 265 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6997 NotI GC'GGCC_GC Cuts at: 4444 4444 Size: 6997 NspI r_CATG'y Cuts at: 1132 1229 4054 4355 5900 1132 Size: 97 2825 301 1545 2229 Fragments arranged by size: 2825 2229 1545 301 97 PasI CC'CwG_GG Cuts at: 1348 2779 2860 3943 1348 Size: 1431 81 1083 4402 Fragments arranged by size: 4402 1431 1083 81 PciI A'CATG_T Cuts at: 5896 5896 Size: 6997 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6997 PfoI T'CCnGG_A Cuts at: 2265 2265 Size: 6997 PmeI GTTT'AAAC Cuts at: 4301 4301 Size: 6997 PmlI CAC'GTG Cuts at: 2121 2121 Size: 6997 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3378 3410 5066 5098 6579 6611 3378 Size: 32 1656 32 1481 32 3764 Fragments arranged by size: 3764 1656 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2393 3442 3711 2393 Size: 1049 269 5679 Fragments arranged by size: 5679 1049 269 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6997 PsiI TTA'TAA Cuts at: 4567 4567 Size: 6997 PspOMI G'GGCC_C Cuts at: 2782 2871 3171 2782 Size: 89 300 6608 Fragments arranged by size: 6608 300 89 PstI C_TGCA'G Cuts at: 830 3006 3673 4349 830 Size: 2176 667 676 3478 Fragments arranged by size: 3478 2176 676 667 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6997 PvuII CAG'CTG Cuts at: 2302 3001 2302 Size: 699 6298 SalI G'TCGA_C Cuts at: 1936 4010 4339 1936 Size: 2074 329 4594 Fragments arranged by size: 4594 2074 329 SanDI GG'GwC_CC Cuts at: 3711 3711 Size: 6997 SapI GCTCTTCn'nnn_ Cuts at: 2018 2018 Size: 6997 SbfI CC_TGCA'GG Cuts at: 4349 4349 Size: 6997 ScaI AGT'ACT Cuts at: 2165 3152 5245 6957 2165 Size: 987 2093 1712 2205 Fragments arranged by size: 2205 2093 1712 987 SexAI A'CCwGG_T Cuts at: 3600 3600 Size: 6997 SfcI C'TryA_G Cuts at: 826 1045 3002 3669 4033 4345 5480 6161 6352 Size: 219 1957 667 364 312 1135 681 191 Cuts at: 6352 826 Size: 1471 Fragments arranged by size: 1957 1471 1135 681 667 364 312 219 191 SfiI GGCCn_nnn'nGGCC Cuts at: 4271 4271 Size: 6997 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6997 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6997 SmaI CCC'GGG Cuts at: 1872 4324 1872 Size: 2452 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3395 3437 5101 6002 6264 820 Size: 197 926 1452 42 1664 901 262 1553 Fragments arranged by size: 1664 1553 1452 926 901 262 197 42 SnaBI TAC'GTA Cuts at: 493 4788 493 Size: 4295 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6997 SphI G_CATG'C Cuts at: 1132 4355 1132 Size: 3223 3774 SspI AAT'ATT Cuts at: 5 52 4921 5 Size: 47 4869 2081 Fragments arranged by size: 4869 2081 47 StuI AGG'CCT Cuts at: 1517 2358 2514 2946 3026 3551 1517 Size: 841 156 432 80 525 4963 Fragments arranged by size: 4963 841 525 432 156 80 StyI C'CwwG_G Cuts at: 513 1065 4209 4436 513 Size: 552 3144 227 3074 Fragments arranged by size: 3144 3074 552 227 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2149 4029 5024 5041 5194 5379 5824 Size: 1255 569 1880 995 17 153 185 445 Cuts at: 5824 325 Size: 1498 Fragments arranged by size: 1880 1498 1255 995 569 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2163 2499 3150 Size: 274 80 33 51 1443 186 336 651 Cuts at: 3150 5243 6955 96 Size: 2093 1712 138 Fragments arranged by size: 2093 1712 1443 651 336 274 186 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2047 2079 3087 3119 6957 6989 2047 Size: 32 1008 32 3838 32 2055 Fragments arranged by size: 3838 2055 1008 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2846 1316 Size: 1530 5467 XbaI T'CTAG_A Cuts at: 4333 4333 Size: 6997 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2915 4093 2915 Size: 1178 5819 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6997 XmaI C'CCGG_G Cuts at: 1870 4322 1870 Size: 2452 4545 XmnI GAAnn'nnTTC Cuts at: 5126 5126 Size: 6997 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4805 276 Size: 53 83 186 936 3271 2468 Fragments arranged by size: 3271 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SalI SanDI SapI SbfI ScaI SexAI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI AvrII BbeI BbvCI BfrBI Bpu10I BstZ17I DraIII EcoICRI EcoRV FalI FseI FspAI KasI NarI NruI NsiI PacI PspXI PsrI RsrII SacI SacII SfoI SrfI SwaI