(Linear) (Six-base) MAPSORT of: KIEE2089.seq Check: 8716 from: 1 to: 2292 KIEE2089.orf With 182 enzymes: SgfI * July 15, 2010 18:54 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 28 2292 Size: 28 2264 AccI GT'mk_AC Cuts at: 0 416 2006 2292 Size: 416 1590 286 Fragments arranged by size: 1590 416 286 Acc65I G'GTAC_C Cuts at: 0 1395 2292 Size: 1395 897 AfeI AGC'GCT Cuts at: 0 1916 2292 Size: 1916 376 AleI CACnn'nnGTG Cuts at: 0 277 1072 1414 1930 2292 Size: 277 795 342 516 362 Fragments arranged by size: 795 516 362 342 277 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 907 2292 Size: 907 1385 AlwNI CAG_nnn'CTG Cuts at: 0 603 1515 1738 2292 Size: 603 912 223 554 Fragments arranged by size: 912 603 554 223 ApaI G_GGCC'C Cuts at: 0 781 870 1170 2292 Size: 781 89 300 1122 Fragments arranged by size: 1122 781 300 89 ApoI r'AATT_y Cuts at: 0 802 1459 2071 2292 Size: 802 657 612 221 Fragments arranged by size: 802 657 612 221 AvaI C'yCGr_G Cuts at: 0 73 718 724 2292 Size: 73 645 6 1568 Fragments arranged by size: 1568 645 73 6 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 0 1614 1647 2292 Size: 1614 33 645 Fragments arranged by size: 1614 645 33 BamHI G'GATC_C Cuts at: 0 887 1800 2292 Size: 887 913 492 Fragments arranged by size: 913 887 492 BanI G'GyrC_C Cuts at: 0 164 1264 1395 1932 2292 Size: 164 1100 131 537 360 Fragments arranged by size: 1100 537 360 164 131 BanII G_rGCy'C Cuts at: 0 398 725 754 781 870 981 1170 1989 Size: 398 327 29 27 89 111 189 819 Cuts at: 1989 2292 Size: 303 Fragments arranged by size: 819 398 327 303 189 111 89 29 27 BbsI GAAGACnn'nnnn_ Cuts at: 0 27 746 2292 Size: 27 719 1546 Fragments arranged by size: 1546 719 27 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1530 1564 2292 Size: 1530 34 728 Fragments arranged by size: 1530 728 34 BclI T'GATC_A Cuts at: 0 932 2292 Size: 932 1360 BglI GCCn_nnn'nGGC Cuts at: 0 2221 2266 2292 Size: 2221 45 26 Fragments arranged by size: 2221 45 26 BlpI GC'TnA_GC Cuts at: 0 298 634 2292 Size: 298 336 1658 Fragments arranged by size: 1658 336 298 Bme1580I G_kGCm'C Cuts at: 0 774 781 870 1170 1937 2292 Size: 774 7 89 300 767 355 Fragments arranged by size: 774 767 355 300 89 7 BmgBI CAC'GTC Cuts at: 0 840 2292 Size: 840 1452 BmrI ACTGGGnnnn_n' Cuts at: 0 1215 2292 Size: 1215 1077 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 1447 2292 Size: 1447 845 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 207 394 519 1267 1365 1893 2049 2227 Size: 207 187 125 748 98 528 156 178 Cuts at: 2227 2292 Size: 65 Fragments arranged by size: 748 528 207 187 178 156 125 98 65 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1375 1417 2292 Size: 1375 42 875 Fragments arranged by size: 1375 875 42 BsaI GGTCTCn'nnnn_ Cuts at: 0 1926 2164 2292 Size: 1926 238 128 Fragments arranged by size: 1926 238 128 BsaAI yAC'GTr Cuts at: 0 116 2292 Size: 116 2176 BsaHI Gr'CG_yC Cuts at: 0 1304 2292 Size: 1304 988 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 1082 1112 2292 Size: 1082 30 1180 Fragments arranged by size: 1180 1082 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 91 195 1057 1264 1455 2292 Size: 91 104 862 207 191 837 Fragments arranged by size: 862 837 207 191 104 91 BseYI C'CCAG_C Cuts at: 0 39 293 643 664 870 1419 1519 1537 Size: 39 254 350 21 206 549 100 18 Cuts at: 1537 2292 Size: 755 Fragments arranged by size: 755 549 350 254 206 100 39 21 18 BsiEI CG_ry'CG Cuts at: 0 2010 2292 Size: 2010 282 BsiHKAI G_wGCw'C Cuts at: 0 17 1109 2164 2292 Size: 17 1092 1055 128 Fragments arranged by size: 1092 1055 128 17 BsiWI C'GTAC_G Cuts at: 0 1469 2292 Size: 1469 823 BsmI GAATG_Cn' Cuts at: 0 477 699 2292 Size: 477 222 1593 Fragments arranged by size: 1593 477 222 BsmBI CGTCTCn'nnnn_ Cuts at: 0 225 2292 Size: 225 2067 Bsp1286I G_dGCh'C Cuts at: 0 17 398 725 754 774 781 870 981 Size: 17 381 327 29 20 7 89 111 Cuts at: 981 1109 1170 1937 1989 2164 2292 Size: 128 61 767 52 175 128 Fragments arranged by size: 767 381 327 175 128 128 111 89 61 52 29 20 17 7 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 28 2292 Size: 28 2264 BsrDI GCAATG_nn' Cuts at: 0 287 2292 Size: 287 2005 BsrFI r'CCGG_y Cuts at: 0 143 167 1306 1571 2292 Size: 143 24 1139 265 721 Fragments arranged by size: 1139 721 265 143 24 BsrGI T'GTAC_A Cuts at: 0 494 2292 Size: 494 1798 BstAPI GCAn_nnn'nTGC Cuts at: 0 1738 2292 Size: 1738 554 BstEII G'GTnAC_C Cuts at: 0 441 1599 2292 Size: 441 1158 693 Fragments arranged by size: 1158 693 441 BstXI CCAn_nnnn'nTGG Cuts at: 0 391 988 1654 1917 2256 2292 Size: 391 597 666 263 339 36 Fragments arranged by size: 666 597 391 339 263 36 BstYI r'GATC_y Cuts at: 0 887 1800 2292 Size: 887 913 492 Fragments arranged by size: 913 887 492 Bsu36I CC'TnA_GG Cuts at: 0 745 782 943 2292 Size: 745 37 161 1349 Fragments arranged by size: 1349 745 161 37 BtgI C'CryG_G Cuts at: 0 1355 1964 2255 2292 Size: 1355 609 291 37 Fragments arranged by size: 1355 609 291 37 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 653 1727 2292 Size: 653 1074 565 Fragments arranged by size: 1074 653 565 BtsI GCAGTG_nn' Cuts at: 0 828 1961 2292 Size: 828 1133 331 Fragments arranged by size: 1133 828 331 DrdI GACnn_nn'nnGTC Cuts at: 0 412 844 2292 Size: 412 432 1448 Fragments arranged by size: 1448 432 412 EaeI y'GGCC_r Cuts at: 0 594 797 1100 1176 1979 2258 2292 Size: 594 203 303 76 803 279 34 Fragments arranged by size: 803 594 303 279 203 76 34 EarI CTCTTCn'nnn_ Cuts at: 0 13 49 856 1036 1682 2106 2132 2292 Size: 13 36 807 180 646 424 26 160 Fragments arranged by size: 807 646 424 180 160 36 26 13 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 207 394 519 1267 1365 1893 2049 2227 Size: 207 187 125 748 98 528 156 178 Cuts at: 2227 2292 Size: 65 Fragments arranged by size: 748 528 207 187 178 156 125 98 65 EcoNI CCTnn'n_nnAGG Cuts at: 0 946 1246 1726 2292 Size: 946 300 480 566 Fragments arranged by size: 946 566 480 300 EcoO109I rG'GnC_Cy Cuts at: 0 388 778 866 867 1166 1437 1512 1534 Size: 388 390 88 1 299 271 75 22 Cuts at: 1534 1706 2292 Size: 172 586 Fragments arranged by size: 586 390 388 299 271 172 88 75 22 1 HaeII r_GCGC'y Cuts at: 0 321 966 1918 2292 Size: 321 645 952 374 Fragments arranged by size: 952 645 374 321 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 1904 1936 2003 2035 2292 Size: 1904 32 67 32 257 Fragments arranged by size: 1904 257 67 32 32 HincII GTy'rAC Cuts at: 0 141 849 2007 2292 Size: 141 708 1158 285 Fragments arranged by size: 1158 708 285 141 KpnI G_GTAC'C Cuts at: 0 1399 2292 Size: 1399 893 MscI TGG'CCA Cuts at: 0 596 799 1981 2292 Size: 596 203 1182 311 Fragments arranged by size: 1182 596 311 203 MslI CAynn'nnrTG Cuts at: 0 277 986 1072 1414 1652 1930 2044 2292 Size: 277 709 86 342 238 278 114 248 Fragments arranged by size: 709 342 278 277 248 238 114 86 MspA1I CmG'CkG Cuts at: 0 297 996 1181 1702 2292 Size: 297 699 185 521 590 Fragments arranged by size: 699 590 521 297 185 NaeI GCC'GGC Cuts at: 0 169 1308 1573 2292 Size: 169 1139 265 719 Fragments arranged by size: 1139 719 265 169 NgoMIV G'CCGG_C Cuts at: 0 167 1306 1571 2292 Size: 167 1139 265 721 Fragments arranged by size: 1139 721 265 167 NspI r_CATG'y Cuts at: 0 2049 2292 Size: 2049 243 PasI CC'CwG_GG Cuts at: 0 774 855 1938 2292 Size: 774 81 1083 354 Fragments arranged by size: 1083 774 354 81 PfoI T'CCnGG_A Cuts at: 0 260 2292 Size: 260 2032 PmlI CAC'GTG Cuts at: 0 116 2292 Size: 116 2176 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 1373 1405 2292 Size: 1373 32 887 Fragments arranged by size: 1373 887 32 PpuMI rG'GwC_Cy Cuts at: 0 388 1437 1706 2292 Size: 388 1049 269 586 Fragments arranged by size: 1049 586 388 269 PspOMI G'GGCC_C Cuts at: 0 777 866 1166 2292 Size: 777 89 300 1126 Fragments arranged by size: 1126 777 300 89 PstI C_TGCA'G Cuts at: 0 1001 1668 2292 Size: 1001 667 624 Fragments arranged by size: 1001 667 624 PvuII CAG'CTG Cuts at: 0 297 996 2292 Size: 297 699 1296 Fragments arranged by size: 1296 699 297 SalI G'TCGA_C Cuts at: 0 2005 2292 Size: 2005 287 SanDI GG'GwC_CC Cuts at: 0 1706 2292 Size: 1706 586 SapI GCTCTTCn'nnn_ Cuts at: 0 13 2292 Size: 13 2279 ScaI AGT'ACT Cuts at: 0 160 1147 2292 Size: 160 987 1145 Fragments arranged by size: 1145 987 160 SexAI A'CCwGG_T Cuts at: 0 1595 2292 Size: 1595 697 SfcI C'TryA_G Cuts at: 0 997 1664 2028 2292 Size: 997 667 364 264 Fragments arranged by size: 997 667 364 264 SfiI GGCCn_nnn'nGGCC Cuts at: 0 2266 2292 Size: 2266 26 SmlI C'TyrA_G Cuts at: 0 1390 1432 2292 Size: 1390 42 860 Fragments arranged by size: 1390 860 42 StuI AGG'CCT Cuts at: 0 353 509 941 1021 1546 2292 Size: 353 156 432 80 525 746 Fragments arranged by size: 746 525 432 353 156 80 StyI C'CwwG_G Cuts at: 0 2204 2292 Size: 2204 88 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 144 2024 2292 Size: 144 1880 268 Fragments arranged by size: 1880 268 144 TatI w'GTAC_w Cuts at: 0 158 494 1145 2292 Size: 158 336 651 1147 Fragments arranged by size: 1147 651 336 158 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 42 74 1082 1114 2292 Size: 42 32 1008 32 1178 Fragments arranged by size: 1178 1008 42 32 32 Tth111I GACn'n_nGTC Cuts at: 0 841 2292 Size: 841 1451 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 910 2088 2292 Size: 910 1178 204 Fragments arranged by size: 1178 910 204 Enzymes that do cut: AarI AccI Acc65I AfeI AleI AlfI AlwNI ApaI ApoI AvaI BaeI BamHI BanI BanII BbsI BcgI BclI BglI BlpI Bme1580I BmgBI BmrI BplI BpmI BpuEI BsaI BsaAI BsaHI BsaXI BseRI BseYI BsiEI BsiHKAI BsiWI BsmI BsmBI Bsp1286I BspMI BsrDI BsrFI BsrGI BstAPI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI DrdI EaeI EarI Eco57MI EcoNI EcoO109I HaeII Hin4I HincII KpnI MscI MslI MspA1I NaeI NgoMIV NspI PasI PfoI PmlI PpiI PpuMI PspOMI PstI PvuII SalI SanDI SapI ScaI SexAI SfcI SfiI SmlI StuI StyI TaqII TatI TstI Tth111I XcmI Enzymes that do not cut: AatII AclI AcuI AflII AflIII AgeI AhdI AloI ApaLI AscI AseI AsiSI AvrII BbeI BbvCI BciVI BfrBI BglII BmtI Bpu10I BsaBI BsaWI BsgI BspEI BspHI BsrBI BssHII BssSI BstBI BstZ17I ClaI CspCI DraI DraIII EagI EciI EcoICRI EcoRI EcoRV FalI FseI FspI FspAI HindIII HpaI KasI MfeI MluI MmeI NarI NcoI NdeI NheI NotI NruI NsiI PacI PciI PflMI PmeI PshAI PsiI PspXI PsrI PvuI RsrII SacI SacII SbfI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI SwaI XbaI XhoI XmaI XmnI ZraI