################################## # NO CUT ENZYMES ( 187 enzymes) # ################################## AatII Acc65I AccI AccII AciI AclI AcsI AcyI AfaI AflIII AgeI AhdI Alw44I AlwI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AvaI AviII AvrII BalI BamHI BanI BanII BbeI BbrPI BbvCI BcgI BciVI BclI BcnI BglI BglII BlnI BlpI BmyI BpmI Bpu1102I BsaAI BsaBI BsaHI BsaI BsaJI BsgI BsiEI BsiHKAI BsiWI BsmBI BsmFI BsmI BsoBI Bsp1286I Bsp1407I BspHI BspLU11I BspMI BsrBI BsrFI BsrGI BssHII BssKI BssSI Bst1107I BstAPI BstBI BstEII BstNI BstPI BstUI BstXI BstYI BstZ17I CelII Cfr10I CpoI DraIII DrdI DsaI EaeI EagI Eam1105I EarI EclXI Eco47III Eco52I EcoNI EcoO65I EcoRI EcoRII EcoRV EcoT14I EcoT22I FauI FbaI FseI FspI HaeII HaeIII HgaI Hin1I HincII HindII HindIII HpaI HphI KasI KpnI Ksp632I KspI MaeII MamI MfeI MflI MluI MluNI MscI MslI MspA1I MunI MvaI MvnI NaeI NarI NciI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflMI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsaI RsrII SacI SacII SalI SapI SbfI ScaI ScrFI SexAI SfcI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI StuI StyI SwaI Van91I XbaI XcmI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 41 enzymes) # ################################### AccIII 317 TCCGGA T^CCGGA AflII 192 CTTAAG C^TTAAG AlwNI 430 CAGATTCTG CAG[ACGT][ACGT][ACGT]^CTG AocI 460 CCTCAGG CC^T[ACGT]AGG AspI 201 GACTGAGTC GAC[ACGT]^[ACGT][ACGT]GTC AvaII 363 GGACC G^G[AT]CC BbsI 75 GAAGAC GAAGAC(2/6) BfrI 192 CTTAAG C^TTAAG BpuAI 75 GAAGAC GAAGAC(2/6) BsaWI 317 TCCGGA [AT]^CCGG[AT] BseAI 317 TCCGGA T^CCGGA BseRI 517 GAGGAG GAGGAG(10/8) BsmAI 520 GAGAC GTCTC(1/5) BspDI 233 ATCGAT AT^CGAT BspEI 317 TCCGGA T^CCGGA BsrI 67 ACTGG ACTGG(1/-1) BstF5I 512 GGATG GGATG(2/0) Bsu36I 460 CCTCAGG CC^T[ACGT]AGG CfoI 249 GCGC GCG^C Cfr13I 363 GGACC G^G[ACGT]CC ClaI 233 ATCGAT AT^CGAT DraI 283 TTTAAA TTT^AAA DraII 362 AGGACCT [GA]G^G[ACGT]CC[CT] Eco81I 460 CCTCAGG CC^T[ACGT]AGG EcoO109I 362 AGGACCT [GA]G^G[ACGT]CC[CT] FokI 512 GGATG GGATG(9/13) HhaI 249 GCGC GCG^C HinP1I 249 GCGC G^CGC MaeIII 440 GTCAC ^GT[ACGT]AC MroI 317 TCCGGA T^CCGGA NlaIV 456 GGAACC GG[ACGT]^[ACGT]CC NspI 391 ACATGC [GA]CATG^[CT] PflFI 201 GACTGAGTC GAC[ACGT]^[ACGT][ACGT]GTC PpuMI 362 AGGACCT [GA]G^G[AT]CC[CT] Sau96I 363 GGACC G^G[ACGT]CC SfaNI 236 GATGC GCATC(5/9) SmlI 192 CTTAAG C^T[CT][GA]AG TaqI 234 TCGA T^CGA Tsp45I 440 GTCAC ^GT[GC]AC Tth111I 201 GACTGAGTC GAC[ACGT]^[ACGT][ACGT]GTC TthHB8I 234 TCGA T^CGA ################################### # TWO CUT ENZYMES ( 19 enzymes) # ################################### AluI 94 AGCT AG^CT 452 AGCT BfaI 11 CTAG C^TAG 493 CTAG BsiYI 61 CCTTCCACTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 396 CCATTGTGGGG BslI 61 CCTTCCACTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 396 CCATTGTGGGG BsrDI 412 GCAATG GCAATG(2/0) 507 GCAATG Cac8I 159 GCAAGC GC[ACGT]^[ACGT]GC 225 GCCTGC DpnII 102 GATC ^GATC 324 GATC HapII 318 CCGG C^CGG 369 CCGG HpaII 318 CCGG C^CGG 369 CCGG MaeI 11 CTAG C^TAG 493 CTAG MboI 102 GATC ^GATC 324 GATC MseI 193 TTAA T^TAA 284 TTAA MspI 318 CCGG C^CGG 369 CCGG MwoI 242 GCCTCCTGCGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC 249 GCGCCATTTGC NdeII 102 GATC ^GATC 324 GATC Sau3AI 102 GATC ^GATC 324 GATC Tru9I 193 TTAA T^TAA 284 TTAA Tsp509I 85 AATT ^AATT 340 AATT TspRI 66 CACTG CA[GC]TG[ACGT][ACGT]^ 153 CAGTG ######################################### # 3 OR MORE CUT ENZYMES ( 11 enzymes) # ######################################### BbvI DdeI Fnu4HI HinfI ItaI MboII MnlI NlaIII PleI TfiI TseI