(Circular) (Six-base) MAPSORT of: pFN21AE0686.seq Check: 6378 from: 1 to: 5905 pFN21AE0686 5905 bp With 182 enzymes: SgfI * October 25, 2019 12:32 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3715 278 Size: 53 83 186 936 2179 2468 Fragments arranged by size: 2468 2179 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3248 1937 Size: 1311 4594 Acc65I G'GTAC_C Cuts at: 3226 3226 Size: 5905 AclI AA'CG_TT Cuts at: 1563 4032 4405 1563 Size: 2469 373 3063 Fragments arranged by size: 3063 2469 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2273 3050 3176 3968 5352 5620 1632 Size: 641 777 126 792 1384 268 1917 Fragments arranged by size: 1917 1384 792 777 641 268 126 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5708 AflIII A'CryG_T Cuts at: 1203 2739 4804 5781 1203 Size: 1536 2065 977 1327 Fragments arranged by size: 2065 1536 1327 977 AgeI A'CCGG_T Cuts at: 4736 4736 Size: 5905 AhdI GACnn_n'nnGTC Cuts at: 2743 4634 2743 Size: 1891 4014 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5905 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2255 1184 Size: 1071 4834 AlwNI CAG_nnn'CTG Cuts at: 2212 2533 3648 5220 2212 Size: 321 1115 1572 2897 Fragments arranged by size: 2897 1572 1115 321 ApaI G_GGCC'C Cuts at: 3181 3181 Size: 5905 ApaLI G'TGCA_C Cuts at: 3962 5118 3962 Size: 1156 4749 ApoI r'AATT_y Cuts at: 1493 2270 2858 3214 3440 3592 5701 1493 Size: 777 588 356 226 152 2109 1697 Fragments arranged by size: 2109 1697 777 588 356 226 152 AseI AT'TA_AT Cuts at: 160 1032 4459 160 Size: 872 3427 1606 Fragments arranged by size: 3427 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5905 AvaI C'yCGr_G Cuts at: 1870 1943 3174 3230 1870 Size: 73 1231 56 4545 Fragments arranged by size: 4545 1231 73 56 BamHI G'GATC_C Cuts at: 3235 3602 3235 Size: 367 5538 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2038 3226 4681 618 Size: 325 217 627 251 1188 1455 1842 Fragments arranged by size: 1842 1455 1188 627 325 251 217 BanII G_rGCy'C Cuts at: 1391 2033 2934 3181 3224 1391 Size: 642 901 247 43 4072 Fragments arranged by size: 4072 901 642 247 43 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2837 928 Size: 971 938 3996 Fragments arranged by size: 3996 971 938 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 4096 4130 1903 Size: 34 50 34 2075 34 3678 Fragments arranged by size: 3678 2075 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2793 3797 5007 2793 Size: 1004 1210 3691 Fragments arranged by size: 3691 1210 1004 BclI T'GATC_A Cuts at: 1548 1548 Size: 5905 BfrBI ATG'CAT Cuts at: 3137 3137 Size: 5905 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3185 4516 136 Size: 107 122 71 631 605 1513 1331 1525 Fragments arranged by size: 1525 1513 1331 631 605 122 107 71 BglII A'GATC_T Cuts at: 2244 5900 2244 Size: 3656 2249 BlpI GC'TnA_GC Cuts at: 3322 3322 Size: 5905 Bme1580I G_kGCm'C Cuts at: 1790 2348 3181 3966 5122 1790 Size: 558 833 785 1156 2573 Fragments arranged by size: 2573 1156 833 785 558 BmgBI CAC'GTC Cuts at: 1318 2146 1318 Size: 828 5077 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2335 3636 4594 Size: 416 53 83 399 24 918 1301 958 Cuts at: 4594 442 Size: 1753 Fragments arranged by size: 1753 1301 958 918 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5905 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2682 3203 4565 1239 Size: 1443 521 1362 2579 Fragments arranged by size: 2579 1443 1362 521 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4030 4895 5193 4030 Size: 865 298 4742 Fragments arranged by size: 4742 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2460 4568 882 Size: 617 961 2108 2219 Fragments arranged by size: 2219 2108 961 617 BsaAI yAC'GTr Cuts at: 493 1204 3696 493 Size: 711 2492 2702 Fragments arranged by size: 2702 2492 711 BsaBI GATnn'nnATC Cuts at: 5899 5899 Size: 5905 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3712 4094 4729 275 Size: 53 83 186 936 2179 382 635 1451 Fragments arranged by size: 2179 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4338 4736 5010 5157 5568 4338 Size: 398 274 147 411 4675 Fragments arranged by size: 4675 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5680 Fragments arranged by size: 5680 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2478 3080 1186 Size: 1292 602 4011 Fragments arranged by size: 4011 1292 602 BseYI C'CCAG_C Cuts at: 2218 2707 5108 5604 2218 Size: 489 2401 496 2519 Fragments arranged by size: 2519 2401 496 489 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2309 2996 1163 Size: 573 573 687 4072 Fragments arranged by size: 4072 687 573 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3355 4116 5144 1540 Size: 275 186 1354 761 1028 2301 Fragments arranged by size: 2301 1354 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3966 4051 5122 3966 Size: 85 1071 4749 Fragments arranged by size: 4749 1071 85 BsiWI C'GTAC_G Cuts at: 2356 2356 Size: 5905 BsmI GAATG_Cn' Cuts at: 2474 2536 3416 3509 2474 Size: 62 880 93 4870 Fragments arranged by size: 4870 880 93 62 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2033 2348 2934 3181 3224 3966 4051 Size: 399 243 315 586 247 43 742 85 Cuts at: 4051 5122 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 586 399 315 247 243 85 43 BspEI T'CCGG_A Cuts at: 5568 5568 Size: 5905 BspHI T'CATG_A Cuts at: 2502 3794 5524 2502 Size: 1292 1730 2883 Fragments arranged by size: 2883 1730 1292 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3260 844 Size: 2416 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3046 3792 1394 Size: 429 1223 746 3507 Fragments arranged by size: 3507 1223 746 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2547 4400 4574 65 Size: 1183 1299 1853 174 1396 Fragments arranged by size: 1853 1396 1299 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2035 4549 4736 1700 Size: 110 225 2514 187 2869 Fragments arranged by size: 2869 2514 225 187 110 BsrGI T'GTAC_A Cuts at: 96 2763 96 Size: 2667 3238 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5905 BssSI C'ACGA_G Cuts at: 3965 4977 3965 Size: 1012 4893 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5905 BstBI TT'CG_AA Cuts at: 4709 4709 Size: 5905 BstEII G'GTnAC_C Cuts at: 1184 2197 1184 Size: 1013 4892 BstXI CCAn_nnnn'nTGG Cuts at: 3082 3082 Size: 5905 BstYI r'GATC_y Cuts at: 1972 2244 3235 3602 3987 4004 5456 5542 5554 Size: 272 991 367 385 17 1452 86 12 Cuts at: 5554 5900 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 991 385 367 346 272 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2803 513 Size: 552 1738 3615 Fragments arranged by size: 3615 1738 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5166 Fragments arranged by size: 5166 683 56 BtsI GCAGTG_nn' Cuts at: 3008 3421 4215 4235 3008 Size: 413 794 20 4678 Fragments arranged by size: 4678 794 413 20 ClaI AT'CG_AT Cuts at: 1554 2184 1554 Size: 630 5275 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5870 DraI TTT'AAA Cuts at: 3209 3209 Size: 5905 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2195 4912 809 Size: 507 879 2717 1802 Fragments arranged by size: 2717 1802 879 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3084 3352 4241 Size: 54 1425 50 275 18 1254 268 889 Cuts at: 4241 5870 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1254 889 275 268 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3352 1537 Size: 275 1540 4090 Fragments arranged by size: 4090 1540 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2501 3152 3835 1353 Size: 1148 651 683 3423 Fragments arranged by size: 3423 1148 683 651 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3178 4492 4866 5012 387 Size: 1064 138 1589 1314 374 146 1280 Fragments arranged by size: 1589 1314 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2273 2682 3050 3176 3203 3968 4565 Size: 393 641 409 368 126 27 765 597 Cuts at: 4565 5352 5620 1239 Size: 787 268 1524 Fragments arranged by size: 1524 787 765 641 597 409 393 368 268 126 27 EcoNI CCTnn'n_nnAGG Cuts at: 2593 3032 2593 Size: 439 5466 EcoO109I rG'GnC_Cy Cuts at: 2209 2259 2995 3025 3178 2209 Size: 50 736 30 153 4936 Fragments arranged by size: 4936 736 153 50 30 EcoRI G'AATT_C Cuts at: 3214 3592 3214 Size: 378 5527 FspI TGC'GCA Cuts at: 4411 4411 Size: 5905 HaeII r_GCGC'y Cuts at: 1678 5052 1678 Size: 3374 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2729 2761 4546 4578 4620 Size: 32 135 32 1157 32 1785 32 42 Cuts at: 4620 4652 1373 Size: 32 2626 Fragments arranged by size: 2626 1785 1157 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3100 3249 3495 669 Size: 988 281 1162 149 246 3079 Fragments arranged by size: 3079 1162 988 281 246 149 HindIII A'AGCT_T Cuts at: 748 748 Size: 5905 HpaI GTT'AAC Cuts at: 3495 3495 Size: 5905 KpnI G_GTAC'C Cuts at: 3230 3230 Size: 5905 MfeI C'AATT_G Cuts at: 3504 3504 Size: 5905 MluI A'CGCG_T Cuts at: 5781 5781 Size: 5905 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2037 2823 3165 5019 5203 1305 Size: 732 786 342 1854 184 2007 Fragments arranged by size: 2007 1854 786 732 342 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3086 10 Size: 54 1425 343 1254 2829 Fragments arranged by size: 2829 1425 1254 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2559 2843 3863 4222 4381 518 Size: 648 1393 284 1020 359 159 2042 Fragments arranged by size: 2042 1393 1020 648 359 284 159 MspA1I CmG'CkG Cuts at: 1606 3321 3998 5146 5391 1606 Size: 1715 677 1148 245 2120 Fragments arranged by size: 2120 1715 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5353 NdeI CA'TA_TG Cuts at: 387 387 Size: 5905 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5905 NotI GC'GGCC_GC Cuts at: 3352 3352 Size: 5905 NsiI A_TGCA'T Cuts at: 3139 3139 Size: 5905 NspI r_CATG'y Cuts at: 1132 1229 2743 3263 4808 1132 Size: 97 1514 520 1545 2229 Fragments arranged by size: 2229 1545 1514 520 97 PasI CC'CwG_GG Cuts at: 1348 2020 2749 1348 Size: 672 729 4504 Fragments arranged by size: 4504 729 672 PciI A'CATG_T Cuts at: 2739 4804 2739 Size: 2065 3840 PflMI CCAn_nnn'nTGG Cuts at: 1108 2212 1108 Size: 1104 4801 PfoI T'CCnGG_A Cuts at: 2454 2454 Size: 5905 PmeI GTTT'AAAC Cuts at: 3209 3209 Size: 5905 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3974 4006 5487 5519 3974 Size: 32 1481 32 4360 Fragments arranged by size: 4360 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2209 2259 2995 3025 2209 Size: 50 736 30 5089 Fragments arranged by size: 5089 736 50 30 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5905 PsiI TTA'TAA Cuts at: 3475 3475 Size: 5905 PspOMI G'GGCC_C Cuts at: 3177 3177 Size: 5905 PstI C_TGCA'G Cuts at: 830 2163 3257 830 Size: 1333 1094 3478 Fragments arranged by size: 3478 1333 1094 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5905 SalI G'TCGA_C Cuts at: 1936 3247 1936 Size: 1311 4594 SapI GCTCTTCn'nnn_ Cuts at: 2501 2501 Size: 5905 SbfI CC_TGCA'GG Cuts at: 3257 3257 Size: 5905 ScaI AGT'ACT Cuts at: 4153 5865 4153 Size: 1712 4193 SfcI C'TryA_G Cuts at: 826 1045 2159 3253 4388 5069 5260 826 Size: 219 1114 1094 1135 681 191 1471 Fragments arranged by size: 1471 1135 1114 1094 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5905 SgrAI Cr'CCGG_yG Cuts at: 1700 2035 1700 Size: 335 5570 SmaI CCC'GGG Cuts at: 1872 3232 1872 Size: 1360 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4009 4910 5172 820 Size: 197 926 2066 901 262 1553 Fragments arranged by size: 2066 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3696 493 Size: 3203 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5905 SphI G_CATG'C Cuts at: 1132 3263 1132 Size: 2131 3774 SspI AAT'ATT Cuts at: 5 52 3829 5 Size: 47 3777 2081 Fragments arranged by size: 3777 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5905 StyI C'CwwG_G Cuts at: 513 1065 2301 2637 2972 3081 3344 513 Size: 552 1236 336 335 109 263 3074 Fragments arranged by size: 3074 1236 552 336 335 263 109 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2741 2951 3932 3949 4102 4287 4732 Size: 1255 1161 210 981 17 153 185 445 Cuts at: 4732 325 Size: 1498 Fragments arranged by size: 1498 1255 1161 981 445 210 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2763 4151 5863 Size: 274 80 33 51 1443 786 1388 1712 Cuts at: 5863 96 Size: 138 Fragments arranged by size: 1712 1443 1388 786 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2135 2167 5865 5897 2135 Size: 32 3698 32 2143 Fragments arranged by size: 3698 2143 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5905 XbaI T'CTAG_A Cuts at: 3241 3241 Size: 5905 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2209 2209 Size: 5905 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5905 XmaI C'CCGG_G Cuts at: 1870 3230 1870 Size: 1360 4545 XmnI GAAnn'nnTTC Cuts at: 4034 4034 Size: 5905 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3713 276 Size: 53 83 186 936 2179 2468 Fragments arranged by size: 2468 2179 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI BplI Bpu10I BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI PspXI PsrI PvuII RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI