Restriction map for
pFN21AE4001
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# NO CUT ENZYMES ( 164 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcsI AcyI AflII AflIII AgeI
AhdI Alw44I AlwNI AocI ApaI
ApaLI ApoI AscI AseI AsnI
Asp700I Asp718I AspEI AspI AvrII
BalI BamHI BanI BbeI BbrPI
BbsI BciVI BclI BfaI BfrI
BglI BlnI BlpI BpmI Bpu1102I
BpuAI BsaAI BsaBI BsaHI BsaWI
BseAI BsgI BsiEI BsiWI BsmBI
BsmI BspDI BspEI BspHI BspLU11I
BspMI BsrBI BsrDI BsrFI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstXI BstZ17I Bsu36I Cac8I
CelII Cfr10I ClaI CpoI DraI
DraIII DrdI EaeI EagI Eam1105I
EarI EclXI Eco52I Eco81I EcoNI
EcoO65I EcoRI EcoRV EcoT14I EcoT22I
FauI FbaI FseI Hin1I HindIII
HpaI KasI KpnI Ksp632I KspI
MaeI MamI MfeI MluI MluNI
MroI MscI MseI MslI MunI
NaeI NarI NcoI NdeI NgoMIV
NheI NlaIV NotI NruI NsiI
NspV PacI PaeR7I PflFI PflMI
PinAI PleI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PvuI
RcaI RsrII SacII SalI SapI
SbfI SexAI SfiI SfoI SfuI
SgrAI SmaI SmlI SnaBI SpeI
SphI Sse8387I SspI StyI SwaI
Tru9I Tsp509I Tth111I Van91I XbaI
XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 39 enzymes) #
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AlwI 147 GATCC GGATC(4/5)
Aor51HI 248 AGCGCT AGC^GCT
AvaI 90 CTCGGG C^[CT]CG[GA]G
AvaII 459 GGACC G^G[AT]CC
AviII 170 TGCGCA TGC^GCA
BbvCI 352 CCTCAGC CCTCAGC(-5/-2)
BcgI 463 CGAGAGATCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglII 467 AGATCT A^GATCT
BsaI 395 GAGACC GGTCTC(1/5)
BsmAI 395 GAGAC GTCTC(1/5)
BsoBI 90 CTCGGG C^[CT]CG[GA]G
Bsp1407I 278 TGTACA T^GTACA
BsrGI 278 TGTACA T^GTACA
BstYI 467 AGATCT [GA]^GATC[CT]
DraII 38 AGGCCCT [GA]G^G[ACGT]CC[CT]
DsaI 155 CCGTGG C^C[GA][CT]GG
Eco47III 248 AGCGCT AGC^GCT
EcoO109I 38 AGGCCCT [GA]G^G[ACGT]CC[CT]
FspI 170 TGCGCA TGC^GCA
HgaI 121 GCGTC GACGC(5/10)
HincII 109 GTCAAC GT[CT]^[GA]AC
HindII 109 GTCAAC GT[CT]^[GA]AC
HinfI 441 GAATC G^A[ACGT]TC
MaeII 113 ACGT A^CGT
MboII 306 GAAGA GAAGA(8/7)
MflI 467 AGATCT [GA]^GATC[CT]
MspA1I 355 CAGCTG C[AC]G^C[GT]G
MwoI 72 GCTGGTCGGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
PstI 358 CTGCAG CTGCA^G
PvuII 355 CAGCTG CAG^CTG
ScaI 86 AGTACT AGT^ACT
SspBI 278 TGTACA T^GTACA
StuI 314 AGGCCT AGG^CCT
TaqI 541 TCGA T^CGA
TfiI 441 GAATC G^A[AT]TC
TspRI 523 CAGTG CA[GC]TG[ACGT][ACGT]^
TthHB8I 541 TCGA T^CGA
XcmI 272 CCATCGTGTACATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 467 AGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AccII 120 CGCG CG^CG
227 CGCG
AciI 226 CCGC CCGC(-3/-1)
234 CCGC
AfaI 87 GTAC GT^AC
279 GTAC
AspHI 64 GAGCTC G[AT]GC[AT]^C
328 GAGCTC
BanII 64 GAGCTC G[GA]GC[CT]^C
328 GAGCTC
BcnI 369 CCCGG CC^[GC]GG
403 CCGGG
BmyI 64 GAGCTC G[AGT]GC[ACT]^C
328 GAGCTC
BseRI 55 GAGGAG GAGGAG(10/8)
260 GAGGAG
BsiHKAI 64 GAGCTC G[AT]GC[AT]^C
328 GAGCTC
BsmFI 209 GGGAC GGGAC(10/14)
219 GGGAC
Bsp1286I 64 GAGCTC G[AGT]GC[ACT]^C
328 GAGCTC
BsrI 29 ACTGG ACTGG(1/-1)
342 ACTGG
BstUI 120 CGCG CG^CG
227 CGCG
Cfr13I 39 GGCCC G^G[ACGT]CC
459 GGACC
HaeII 248 AGCGCT [GA]GCGC^[CT]
268 AGCGCC
HaeIII 39 GGCC GG^CC
315 GGCC
HapII 370 CCGG C^CGG
403 CCGG
HpaII 370 CCGG C^CGG
403 CCGG
HphI 179 TCACC GGTGA(8/7)
515 TCACC
MspI 370 CCGG C^CGG
403 CCGG
MvnI 120 CGCG CG^CG
227 CGCG
NciI 369 CCCGG CC^[GC]GG
403 CCGGG
NspI 167 ACATGC [GA]CATG^[CT]
239 GCATGT
RsaI 87 GTAC GT^AC
279 GTAC
SacI 64 GAGCTC GAGCT^C
328 GAGCTC
Sau96I 39 GGCCC G^G[ACGT]CC
459 GGACC
SfaNI 289 GATGC GCATC(5/9)
365 GCATC
SfcI 358 CTGCAG C^T[GA][CT]AG
520 CTACAG
Tsp45I 193 GTGAC ^GT[GC]AC
546 GTCAC
#########################################
# 3 OR MORE CUT ENZYMES ( 26 enzymes) #
#########################################
AluI BbvI BsaJI BsiYI BslI
BssKI BstF5I BstNI CfoI DdeI
DpnII EcoRII Fnu4HI FokI HhaI
HinP1I ItaI MaeIII MboI MnlI
MvaI NdeII NlaIII Sau3AI ScrFI
TseI