(Circular) (Six-base) MAPSORT of: pFN21AE9461.seq Check: 5716 from: 1 to: 6148 pFN21AE9461 6148 bp With 182 enzymes: SgfI * December 28, 2011 15:24 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2993 2993 Size: 6148 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3958 278 Size: 53 83 186 936 2422 2468 Fragments arranged by size: 2468 2422 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3105 3491 1937 Size: 1168 386 4594 Fragments arranged by size: 4594 1168 386 Acc65I G'GTAC_C Cuts at: 3469 3469 Size: 6148 AclI AA'CG_TT Cuts at: 1563 4275 4648 1563 Size: 2712 373 3063 Fragments arranged by size: 3063 2712 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2507 4211 5595 5863 1632 Size: 875 1704 1384 268 1917 Fragments arranged by size: 1917 1704 1384 875 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5951 AflIII A'CryG_T Cuts at: 1203 5047 6024 1203 Size: 3844 977 1327 Fragments arranged by size: 3844 1327 977 AgeI A'CCGG_T Cuts at: 4979 4979 Size: 6148 AhdI GACnn_n'nnGTC Cuts at: 4877 4877 Size: 6148 AleI CACnn'nnGTG Cuts at: 1166 3086 3242 1166 Size: 1920 156 4072 Fragments arranged by size: 4072 1920 156 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6148 AlwNI CAG_nnn'CTG Cuts at: 2308 2667 3184 3891 5463 2308 Size: 359 517 707 1572 2993 Fragments arranged by size: 2993 1572 707 517 359 ApaLI G'TGCA_C Cuts at: 4205 5361 4205 Size: 1156 4992 ApoI r'AATT_y Cuts at: 1493 3457 3683 3835 5944 1493 Size: 1964 226 152 2109 1697 Fragments arranged by size: 2109 1964 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4702 160 Size: 872 3670 1606 Fragments arranged by size: 3670 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6148 AvaI C'yCGr_G Cuts at: 1870 1943 2888 3473 1870 Size: 73 945 585 4545 Fragments arranged by size: 4545 945 585 73 BamHI G'GATC_C Cuts at: 3478 3845 3478 Size: 367 5781 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2078 2144 2285 2329 2736 Size: 325 217 627 291 66 141 44 407 Cuts at: 2736 2846 3230 3469 4924 618 Size: 110 384 239 1455 1842 Fragments arranged by size: 1842 1455 627 407 384 325 291 239 217 141 110 66 44 BanII G_rGCy'C Cuts at: 1391 2384 2780 2805 3467 1391 Size: 993 396 25 662 4072 Fragments arranged by size: 4072 993 662 396 25 BbeI G_GCGC'C Cuts at: 3234 3234 Size: 6148 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5177 BbvCI CC'TCA_GC Cuts at: 2875 2875 Size: 6148 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 4339 4373 1903 Size: 34 50 34 2318 34 3678 Fragments arranged by size: 3678 2318 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2376 4040 5250 2376 Size: 1664 1210 3274 Fragments arranged by size: 3274 1664 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 6148 BfrBI ATG'CAT Cuts at: 2862 2862 Size: 6148 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2519 2759 4759 Size: 107 122 71 631 605 847 240 2000 Cuts at: 4759 136 Size: 1525 Fragments arranged by size: 2000 1525 847 631 605 240 122 107 71 BglII A'GATC_T Cuts at: 6143 6143 Size: 6148 BlpI GC'TnA_GC Cuts at: 2060 3565 2060 Size: 1505 4643 Bme1580I G_kGCm'C Cuts at: 1790 2083 2147 2288 2332 2625 2674 2849 3440 Size: 293 64 141 44 293 49 175 591 Cuts at: 3440 4209 5365 1790 Size: 769 1156 2573 Fragments arranged by size: 2573 1156 769 591 293 293 175 141 64 49 44 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6148 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2606 3236 3879 Size: 416 53 83 399 24 1189 630 643 Cuts at: 3879 4837 442 Size: 958 1753 Fragments arranged by size: 1753 1189 958 643 630 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6148 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2656 3400 2656 Size: 744 5404 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3003 4808 1239 Size: 1764 1805 2579 Fragments arranged by size: 2579 1805 1764 Bpu10I CC'TnA_GC Cuts at: 2799 2875 2799 Size: 76 6072 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3353 4273 5138 5436 3353 Size: 920 865 298 4065 Fragments arranged by size: 4065 920 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2074 2105 2641 4811 882 Size: 617 575 31 536 2170 2219 Fragments arranged by size: 2219 2170 617 575 536 31 BsaAI yAC'GTr Cuts at: 493 1204 2695 3939 493 Size: 711 1491 1244 2702 Fragments arranged by size: 2702 1491 1244 711 BsaBI GATnn'nnATC Cuts at: 6142 6142 Size: 6148 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3231 3955 4337 4972 Size: 53 83 186 936 1698 724 382 635 Cuts at: 4972 275 Size: 1451 Fragments arranged by size: 1698 1451 936 724 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2368 4581 4979 5253 5400 5811 2368 Size: 2213 398 274 147 411 2705 Fragments arranged by size: 2705 2213 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3197 3227 3408 3438 1178 Size: 30 165 30 1794 30 181 30 3888 Fragments arranged by size: 3888 1794 181 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2171 2204 2629 3329 1186 Size: 985 33 425 700 4005 Fragments arranged by size: 4005 985 700 425 33 BseYI C'CCAG_C Cuts at: 3271 5351 5847 3271 Size: 2080 496 3572 Fragments arranged by size: 3572 2080 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2072 2192 3058 3100 1163 Size: 573 336 120 866 42 4211 Fragments arranged by size: 4211 866 573 336 120 42 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3598 4359 5387 1540 Size: 275 186 1597 761 1028 2301 Fragments arranged by size: 2301 1597 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2384 2805 4209 4294 5365 2384 Size: 421 1404 85 1071 3167 Fragments arranged by size: 3167 1404 1071 421 85 BsmI GAATG_Cn' Cuts at: 3659 3752 3659 Size: 93 6055 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2083 2147 2288 2332 2384 2625 2674 Size: 399 293 64 141 44 52 241 49 Cuts at: 2674 2780 2805 2849 3440 3467 4209 4294 5365 Size: 106 25 44 591 27 742 85 1071 Cuts at: 5365 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 591 399 293 241 141 106 85 64 52 49 44 44 27 25 BspEI T'CCGG_A Cuts at: 5811 5811 Size: 6148 BspHI T'CATG_A Cuts at: 2865 4037 5767 2865 Size: 1172 1730 3246 Fragments arranged by size: 3246 1730 1172 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2993 3503 844 Size: 2149 510 3489 Fragments arranged by size: 3489 2149 510 BsrBI CCG'CTC Cuts at: 1394 1823 2734 4035 1394 Size: 429 911 1301 3507 Fragments arranged by size: 3507 1301 911 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4643 4817 65 Size: 1183 3395 174 1396 Fragments arranged by size: 3395 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4792 4979 1700 Size: 110 2982 187 2869 Fragments arranged by size: 2982 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6148 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6148 BssSI C'ACGA_G Cuts at: 3053 4208 5220 3053 Size: 1155 1012 3981 Fragments arranged by size: 3981 1155 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2182 3444 794 Size: 1388 1262 3498 Fragments arranged by size: 3498 1388 1262 BstBI TT'CG_AA Cuts at: 4952 4952 Size: 6148 BstEII G'GTnAC_C Cuts at: 1184 2371 1184 Size: 1187 4961 BstXI CCAn_nnnn'nTGG Cuts at: 2248 2788 2248 Size: 540 5608 BstYI r'GATC_y Cuts at: 1972 2349 3478 3845 4230 4247 5699 5785 5797 Size: 377 1129 367 385 17 1452 86 12 Cuts at: 5797 6143 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1129 385 377 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2187 2187 Size: 6148 BtgI C'CryG_G Cuts at: 513 1065 2841 3084 3099 513 Size: 552 1776 243 15 3562 Fragments arranged by size: 3562 1776 552 243 15 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5409 Fragments arranged by size: 5409 683 56 BtsI GCAGTG_nn' Cuts at: 3065 3664 4458 4478 3065 Size: 599 794 20 4735 Fragments arranged by size: 4735 794 599 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6148 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6113 DraI TTT'AAA Cuts at: 3452 3452 Size: 6148 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3379 5155 809 Size: 507 2063 1776 1802 Fragments arranged by size: 2063 1802 1776 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2253 2697 2751 Size: 54 1425 50 275 18 423 444 54 Cuts at: 2751 3595 4484 6113 8 Size: 844 889 1629 43 Fragments arranged by size: 1629 1425 889 844 444 423 275 54 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3595 1537 Size: 275 1783 4090 Fragments arranged by size: 4090 1783 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2483 2978 3391 4078 1353 Size: 1130 495 413 687 3423 Fragments arranged by size: 3423 1130 687 495 413 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2213 4735 5109 5255 387 Size: 1064 138 624 2522 374 146 1280 Fragments arranged by size: 2522 1280 1064 624 374 146 138 EcoICRI GAG'CTC Cuts at: 2382 2803 2382 Size: 421 5727 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2507 3003 4211 4808 5595 5863 1239 Size: 393 875 496 1208 597 787 268 1524 Fragments arranged by size: 1524 1208 875 787 597 496 393 268 EcoNI CCTnn'n_nnAGG Cuts at: 2714 2714 Size: 6148 EcoO109I rG'GnC_Cy Cuts at: 3014 3014 Size: 6148 EcoRI G'AATT_C Cuts at: 3457 3835 3457 Size: 378 5770 FspI TGC'GCA Cuts at: 4654 4654 Size: 6148 HaeII r_GCGC'y Cuts at: 1678 2417 2496 3234 5295 1678 Size: 739 79 738 2061 2531 Fragments arranged by size: 2531 2061 739 738 79 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2302 2334 4789 4821 4863 Size: 32 135 32 730 32 2455 32 42 Cuts at: 4863 4895 1373 Size: 32 2626 Fragments arranged by size: 2626 2455 730 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3492 3738 669 Size: 988 281 1554 246 3079 Fragments arranged by size: 3079 1554 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6148 HpaI GTT'AAC Cuts at: 3738 3738 Size: 6148 KasI G'GCGC_C Cuts at: 3230 3230 Size: 6148 KpnI G_GTAC'C Cuts at: 3473 3473 Size: 6148 MfeI C'AATT_G Cuts at: 3747 3747 Size: 6148 MluI A'CGCG_T Cuts at: 6024 6024 Size: 6148 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3336 5262 5446 1305 Size: 2031 1926 184 2007 Fragments arranged by size: 2031 2007 1926 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2255 2699 2753 10 Size: 54 1425 343 423 444 54 3405 Fragments arranged by size: 3405 1425 444 423 343 54 54 MslI CAynn'nnrTG Cuts at: 518 1166 2771 3086 3146 3242 4106 4465 4624 Size: 648 1605 315 60 96 864 359 159 Cuts at: 4624 518 Size: 2042 Fragments arranged by size: 2042 1605 864 648 359 315 159 96 60 MspA1I CmG'CkG Cuts at: 1606 2818 2992 3271 3564 4241 5389 5634 1606 Size: 1212 174 279 293 677 1148 245 2120 Fragments arranged by size: 2120 1212 1148 677 293 279 245 174 NarI GG'CG_CC Cuts at: 3231 3231 Size: 6148 NcoI C'CATG_G Cuts at: 513 1065 2841 513 Size: 552 1776 3820 Fragments arranged by size: 3820 1776 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6148 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6148 NotI GC'GGCC_GC Cuts at: 3595 3595 Size: 6148 NsiI A_TGCA'T Cuts at: 2864 2864 Size: 6148 NspI r_CATG'y Cuts at: 1132 1229 2935 3506 5051 1132 Size: 97 1706 571 1545 2229 Fragments arranged by size: 2229 1706 1545 571 97 PasI CC'CwG_GG Cuts at: 1348 2674 3408 3433 1348 Size: 1326 734 25 4063 Fragments arranged by size: 4063 1326 734 25 PciI A'CATG_T Cuts at: 5047 5047 Size: 6148 PflMI CCAn_nnn'nTGG Cuts at: 1108 2010 2297 3184 1108 Size: 902 287 887 4072 Fragments arranged by size: 4072 902 887 287 PfoI T'CCnGG_A Cuts at: 3285 3285 Size: 6148 PmeI GTTT'AAAC Cuts at: 3452 3452 Size: 6148 PmlI CAC'GTG Cuts at: 2695 2695 Size: 6148 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4217 4249 5730 5762 4217 Size: 32 1481 32 4603 Fragments arranged by size: 4603 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 3101 1613 Size: 1488 4660 PsiI TTA'TAA Cuts at: 3718 3718 Size: 6148 PstI C_TGCA'G Cuts at: 830 2235 3266 3500 830 Size: 1405 1031 234 3478 Fragments arranged by size: 3478 1405 1031 234 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6148 PvuII CAG'CTG Cuts at: 2818 2992 2818 Size: 174 5974 SacI G_AGCT'C Cuts at: 2384 2805 2384 Size: 421 5727 SalI G'TCGA_C Cuts at: 1936 3490 1936 Size: 1554 4594 SapI GCTCTTCn'nnn_ Cuts at: 2483 2483 Size: 6148 SbfI CC_TGCA'GG Cuts at: 3500 3500 Size: 6148 ScaI AGT'ACT Cuts at: 4396 6108 4396 Size: 1712 4436 SfcI C'TryA_G Cuts at: 826 1045 2231 2345 2386 3262 3496 4631 5312 Size: 219 1186 114 41 876 234 1135 681 Cuts at: 5312 5503 826 Size: 191 1471 Fragments arranged by size: 1471 1186 1135 876 681 234 219 191 114 41 SfoI GGC'GCC Cuts at: 3232 3232 Size: 6148 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6148 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6148 SmaI CCC'GGG Cuts at: 1872 3475 1872 Size: 1603 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3368 4252 5153 5415 820 Size: 197 926 1425 884 901 262 1553 Fragments arranged by size: 1553 1425 926 901 884 262 197 SnaBI TAC'GTA Cuts at: 493 3939 493 Size: 3446 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6148 SphI G_CATG'C Cuts at: 1132 3506 1132 Size: 2374 3774 SspI AAT'ATT Cuts at: 5 52 4072 5 Size: 47 4020 2081 Fragments arranged by size: 4020 2081 47 StuI AGG'CCT Cuts at: 1517 3281 3338 1517 Size: 1764 57 4327 Fragments arranged by size: 4327 1764 57 StyI C'CwwG_G Cuts at: 513 1065 2163 2247 2715 2841 3587 513 Size: 552 1098 84 468 126 746 3074 Fragments arranged by size: 3074 1098 746 552 468 126 84 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2390 2443 2588 2761 3389 4175 4192 Size: 1255 810 53 145 173 628 786 17 Cuts at: 4192 4345 4530 4975 325 Size: 153 185 445 1498 Fragments arranged by size: 1498 1255 810 786 628 445 185 173 153 145 53 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4394 6106 96 Size: 274 80 33 51 1443 2417 1712 138 Fragments arranged by size: 2417 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3408 3440 6108 6140 3408 Size: 32 2668 32 3416 Fragments arranged by size: 3416 2668 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6148 XbaI T'CTAG_A Cuts at: 3484 3484 Size: 6148 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2248 2407 2848 2248 Size: 159 441 5548 Fragments arranged by size: 5548 441 159 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6148 XmaI C'CCGG_G Cuts at: 1870 3473 1870 Size: 1603 4545 XmnI GAAnn'nnTTC Cuts at: 4277 4277 Size: 6148 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3956 276 Size: 53 83 186 936 2422 2468 Fragments arranged by size: 2468 2422 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PshAI PsiI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BsiWI BsmBI BstZ17I DraIII EcoRV FalI FseI FspAI NaeI NgoMIV NruI PacI PpuMI PspOMI PspXI PsrI RsrII SacII SanDI SexAI SfiI SrfI SwaI