Restriction map for
pFN21AB7962
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# NO CUT ENZYMES ( 111 enzymes) #
##################################
AatII Acc65I AclI AflII AflIII
AgeI Alw44I AlwNI AocI ApaI
ApaLI AscI AseI AsnI Asp700I
Asp718I AviII AvrII BamHI BbrPI
BcgI BclI BfaI BfrI BlnI
BpmI BsaAI BsaBI BsiWI BsmBI
BsmI Bsp1407I BspDI BspHI BspLU11I
BsrDI BsrGI BsrI Bst1107I BstBI
BstEII BstPI BstXI BstZ17I Bsu36I
ClaI CpoI DraI DraIII DrdI
Eco81I EcoNI EcoO65I EcoRI EcoRV
EcoT22I FbaI FseI FspI HincII
HindII HindIII HpaI KpnI KspI
MaeI MamI MfeI MluI MunI
NcoI NdeI NheI NotI NruI
NsiI NspV PacI PflMI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PstI PvuI RcaI RsrII
SacII SalI SbfI ScaI SexAI
SfuI SgrAI SmaI SnaBI SpeI
Sse8387I SspBI SspI StuI SwaI
Van91I XbaI XcmI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 49 enzymes) #
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AccI 457 GTCTAC GT^[AC][GT]AC
AcsI 961 AAATTC [GA]^AATT[CT]
AcyI 148 GGCGCC G[GA]^CG[CT]C
AhdI 1012 GACGGCACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI 699 AGCGCT AGC^GCT
ApoI 961 AAATTC [GA]^AATT[CT]
AspEI 1012 GACGGCACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BalI 140 TGGCCA TGG^CCA
BbeI 148 GGCGCC GGCGC^C
BbvCI 1037 CCTCAGC CCTCAGC(-5/-2)
BciVI 582 GTATCC GTATCC(6/5)
BglII 230 AGATCT A^GATCT
BsaHI 148 GGCGCC G[GA]^CG[CT]C
BsaI 562 GAGACC GGTCTC(1/5)
BseRI 103 GAGGAG GAGGAG(10/8)
BsiEI 162 CGGCCG CG[GA][CT]^CG
BsmFI 767 GGGAC GGGAC(10/14)
BsrFI 214 GCCGGC [GA]^CCGG[CT]
BssHII 441 GCGCGC G^CGCGC
BstAPI 350 GCATTTACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Cfr10I 214 GCCGGC [GA]^CCGG[CT]
DraII 884 AGGACCT [GA]G^G[ACGT]CC[CT]
DsaI 506 CCACGG C^C[GA][CT]GG
EagI 162 CGGCCG C^GGCCG
Eam1105I 1012 GACGGCACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 690 GAAGAG CTCTTC(1/4)
EclXI 162 CGGCCG C^GGCCG
Eco47III 699 AGCGCT AGC^GCT
Eco52I 162 CGGCCG C^GGCCG
EcoO109I 884 AGGACCT [GA]G^G[ACGT]CC[CT]
HgaI 169 GACGC GACGC(5/10)
Hin1I 148 GGCGCC G[GA]^CG[CT]C
KasI 148 GGCGCC G^GCGCC
Ksp632I 690 GAAGAG CTCTTC(1/4)
MaeII 1018 ACGT A^CGT
MluNI 140 TGGCCA TGG^CCA
MscI 140 TGGCCA TGG^CCA
MslI 1110 CACTGTAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NaeI 214 GCCGGC GCC^GGC
NarI 148 GGCGCC GG^CGCC
NgoMIV 214 GCCGGC G^CCGGC
PaeR7I 313 CTCGAG C^TCGAG
PpuMI 884 AGGACCT [GA]G^G[AT]CC[CT]
PvuII 592 CAGCTG CAG^CTG
SapI 690 GAAGAGC GCTCTTC(1/4)
SfiI 132 GGCCCGTCTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 148 GGCGCC GGC^GCC
TfiI 1150 GAATC G^A[AT]TC
XhoI 313 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 34 enzymes) #
###################################
AlwI 119 GATCC GGATC(4/5)
71 GGATC
AspHI 322 GAGCTC G[AT]GC[AT]^C
1052 GAGCTC
AspI 451 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
712 GACATCGTC
AvaI 313 CTCGAG C^[CT]CG[GA]G
446 CCCGAG
BbsI 25 GAAGAC GAAGAC(2/6)
795 GAAGAC
BglI 133 GCCCGTCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
407 GCCACCTTGGC
BlpI 186 GCTCAGC GC^T[ACGT]AGC
594 GCTGAGC
Bpu1102I 186 GCTCAGC GC^T[ACGT]AGC
594 GCTGAGC
BpuAI 25 GAAGAC GAAGAC(2/6)
795 GAAGAC
BsgI 388 GTGCAG GTGCAG(16/14)
664 GTGCAG
BsiHKAI 322 GAGCTC G[AT]GC[AT]^C
1052 GAGCTC
BsmAI 449 GAGAC GTCTC(1/5)
562 GAGAC
BsoBI 313 CTCGAG C^[CT]CG[GA]G
446 CCCGAG
BsrBI 184 CCGCTC CCGCTC(-3/-3)
621 GAGCGG
BssSI 953 CACGAG CACGAG(-5/-1)
33 CTCGTG
BstNI 537 CCTGG CC^[AT]GG
630 CCTGG
CelII 186 GCTCAGC GC^T[ACGT]AGC
594 GCTGAGC
EcoRII 537 CCTGG ^CC[AT]GG
630 CCTGG
HaeII 148 GGCGCC [GA]GCGC^[CT]
699 AGCGCT
MaeIII 1117 GTGAC ^GT[ACGT]AC
1126 GTGAC
MseI 1084 TTAA T^TAA
1177 TTAA
MvaI 537 CCTGG CC^[AT]GG
630 CCTGG
NspI 176 GCATGC [GA]CATG^[CT]
806 GCATGC
PflFI 451 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
712 GACATCGTC
SacI 322 GAGCTC GAGCT^C
1052 GAGCTC
SfcI 372 CTACAG C^T[GA][CT]AG
1112 CTGTAG
SmlI 313 CTCGAG C^T[CT][GA]AG
889 CTCAAG
SphI 176 GCATGC GCATG^C
806 GCATGC
TaqI 314 TCGA T^CGA
560 TCGA
Tru9I 1084 TTAA T^TAA
1177 TTAA
Tsp45I 1117 GTGAC ^GT[GC]AC
1126 GTGAC
Tsp509I 721 AATT ^AATT
962 AATT
Tth111I 451 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
712 GACATCGTC
TthHB8I 314 TCGA T^CGA
560 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 64 enzymes) #
#########################################
AccII AccIII AciI AfaI AluI
AvaII BanI BanII BbvI BcnI
BmyI BsaJI BsaWI BseAI BsiYI
BslI Bsp1286I BspEI BspMI BssKI
BstF5I BstUI BstYI Cac8I CfoI
Cfr13I DdeI DpnII EaeI EcoT14I
FauI Fnu4HI FokI HaeIII HapII
HhaI HinP1I HinfI HpaII HphI
ItaI MboI MboII MflI MnlI
MroI MspA1I MspI MvnI MwoI
NciI NdeII NlaIII NlaIV PleI
RsaI Sau3AI Sau96I ScrFI SfaNI
StyI TseI TspRI XhoII