Restriction map for
pFN21AB7962
(Circular) (Six-base) MAPSORT of: pFN21AB7962.seq Check: 7413 from: 1 to: 5896
pFN21AB7962 5896 bp
With 182 enzymes: SgfI *
March 28, 2012 12:36 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2342 2492 2342
Size: 150 5746
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3706 278
Size: 53 83 186 936 2170 2468
Fragments arranged by size:
2468 2170 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2463 3239 1937
Size: 526 776 4594
Fragments arranged by size:
4594 776 526
Acc65I G'GTAC_C
Cuts at: 3217 3217
Size: 5896
AclI AA'CG_TT
Cuts at: 1563 4023 4396 1563
Size: 2460 373 3063
Fragments arranged by size:
3063 2460 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2312 3959 5343 5611 1632
Size: 680 1647 1384 268 1917
Fragments arranged by size:
1917 1647 1384 680 268
AfeI AGC'GCT
Cuts at: 2706 2706
Size: 5896
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5699
AflIII A'CryG_T
Cuts at: 1203 4795 5772 1203
Size: 3592 977 1327
Fragments arranged by size:
3592 1327 977
AgeI A'CCGG_T
Cuts at: 4727 4727
Size: 5896
AhdI GACnn_n'nnGTC
Cuts at: 3022 4625 3022
Size: 1603 4293
AleI CACnn'nnGTG
Cuts at: 1166 3119 1166
Size: 1953 3943
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2418 1184
Size: 1234 4662
AlwNI CAG_nnn'CTG
Cuts at: 3639 5211 3639
Size: 1572 4324
ApaLI G'TGCA_C
Cuts at: 3953 5109 3953
Size: 1156 4740
ApoI r'AATT_y
Cuts at: 1493 2966 3205 3431 3583 5692 1493
Size: 1473 239 226 152 2109 1697
Fragments arranged by size:
2109 1697 1473 239 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4450 160
Size: 872 3418 1606
Fragments arranged by size:
3418 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5896
AvaI C'yCGr_G
Cuts at: 1870 1943 2318 2451 3221 1870
Size: 73 375 133 770 4545
Fragments arranged by size:
4545 770 375 133 73
BamHI G'GATC_C
Cuts at: 3226 3593 3226
Size: 367 5529
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2019 2153 2663 3217 4672
Size: 325 217 627 232 134 510 554 1455
Cuts at: 4672 618
Size: 1842
Fragments arranged by size:
1842 1455 627 554 510 325 232 217
134
BanII G_rGCy'C
Cuts at: 1391 2331 2702 3061 3215 1391
Size: 940 371 359 154 4072
Fragments arranged by size:
4072 940 371 359 154
BbeI G_GCGC'C
Cuts at: 2157 2157
Size: 5896
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2037 2807 928
Size: 971 138 770 4017
Fragments arranged by size:
4017 971 770 138
BbvCI CC'TCA_GC
Cuts at: 3043 3043
Size: 5896
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4087 4121 1903
Size: 34 2150 34 3678
Fragments arranged by size:
3678 2150 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2598 3788 4998 2598
Size: 1190 1210 3496
Fragments arranged by size:
3496 1210 1190
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5896
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2144 2418 4507
Size: 107 122 71 631 605 472 274 2089
Cuts at: 4507 136
Size: 1525
Fragments arranged by size:
2089 1525 631 605 472 274 122 107
71
BglII A'GATC_T
Cuts at: 2235 5891 2235
Size: 3656 2240
BlpI GC'TnA_GC
Cuts at: 2192 2600 3313 2192
Size: 408 713 4775
Fragments arranged by size:
4775 713 408
Bme1580I G_kGCm'C
Cuts at: 1790 2407 2666 3957 5113 1790
Size: 617 259 1291 1156 2573
Fragments arranged by size:
2573 1291 1156 617 259
BmgBI CAC'GTC
Cuts at: 1318 3024 1318
Size: 1706 4190
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3627 4585 442
Size: 416 53 83 399 24 2210 958 1753
Fragments arranged by size:
2210 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5896
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4556 1239
Size: 3317 2579
Bpu10I CC'TnA_GC
Cuts at: 2471 3043 2471
Size: 572 5324
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2879 4021 4886 5184 2879
Size: 1142 865 298 3591
Fragments arranged by size:
3591 1142 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2561 4559 882
Size: 617 1062 1998 2219
Fragments arranged by size:
2219 1998 1062 617
BsaAI yAC'GTr
Cuts at: 493 1204 3687 493
Size: 711 2483 2702
Fragments arranged by size:
2702 2483 711
BsaBI GATnn'nnATC
Cuts at: 5890 5890
Size: 5896
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2154 3703 4085 4720
Size: 53 83 186 936 621 1549 382 635
Cuts at: 4720 275
Size: 1451
Fragments arranged by size:
1549 1451 936 635 621 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2081 2841 3158 4329 4727 5001 5148 5559 2081
Size: 760 317 1171 398 274 147 411 2418
Fragments arranged by size:
2418 1171 760 411 398 317 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2063 2093 1178
Size: 30 165 30 660 30 4981
Fragments arranged by size:
4981 660 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2123 1186
Size: 937 4959
BseYI C'CCAG_C
Cuts at: 2056 2157 2701 2707 5099 5595 2056
Size: 101 544 6 2392 496 2357
Fragments arranged by size:
2392 2357 544 496 101 6
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2414 2690 1163
Size: 573 678 276 4369
Fragments arranged by size:
4369 678 573 276
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2170 3346 4107 5135 1540
Size: 275 186 169 1176 761 1028 2301
Fragments arranged by size:
2301 1176 1028 761 275 186 169
BsiHKAI G_wGCw'C
Cuts at: 2331 3061 3957 4042 5113 2331
Size: 730 896 85 1071 3114
Fragments arranged by size:
3114 1071 896 730 85
BsmI GAATG_Cn'
Cuts at: 3407 3500 3407
Size: 93 5803
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2331 2407 2666 2702 3061 3215 3957
Size: 399 541 76 259 36 359 154 742
Cuts at: 3957 4042 5113 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 541 399 359 259 154
85 76 36
BspEI T'CCGG_A
Cuts at: 2081 2841 3158 5559 2081
Size: 760 317 2401 2418
Fragments arranged by size:
2418 2401 760 317
BspHI T'CATG_A
Cuts at: 3785 5515 3785
Size: 1730 4166
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2342 2492 2929 3251 844
Size: 1498 150 437 322 3489
Fragments arranged by size:
3489 1498 437 322 150
BsrBI CCG'CTC
Cuts at: 1394 1823 2191 2628 3783 1394
Size: 429 368 437 1155 3507
Fragments arranged by size:
3507 1155 437 429 368
BsrDI GCAATG_nn'
Cuts at: 65 1248 4391 4565 65
Size: 1183 3143 174 1396
Fragments arranged by size:
3143 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2219 4540 4727 1700
Size: 110 409 2321 187 2869
Fragments arranged by size:
2869 2321 409 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5896
BssHII G'CGCG_C
Cuts at: 1925 2446 1925
Size: 521 5375
BssSI C'ACGA_G
Cuts at: 2038 2958 3956 4968 2038
Size: 920 998 1012 2966
Fragments arranged by size:
2966 1012 998 920
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2361 794
Size: 1567 4329
BstBI TT'CG_AA
Cuts at: 4700 4700
Size: 5896
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5896
BstYI r'GATC_y
Cuts at: 1972 2076 2123 2235 3226 3593 3978 3995 5447
Size: 104 47 112 991 367 385 17 1452
Cuts at: 5447 5533 5545 5891 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 991 385 367 346 112 104
86 47 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2511 513
Size: 552 939 507 3898
Fragments arranged by size:
3898 939 552 507
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5157
Fragments arranged by size:
5157 683 56
BtsI GCAGTG_nn'
Cuts at: 3075 3412 4206 4226 3075
Size: 337 794 20 4745
Fragments arranged by size:
4745 794 337 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5896
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5861
DraI TTT'AAA
Cuts at: 3200 3200
Size: 5896
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4903 809
Size: 507 3587 1802
Fragments arranged by size:
3587 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2145 2167 2619
Size: 54 1425 50 275 18 315 22 452
Cuts at: 2619 3343 4232 5861 8
Size: 724 889 1629 43
Fragments arranged by size:
1629 1425 889 724 452 315 275 54
50 43 22 18
EagI C'GGCC_G
Cuts at: 1537 1812 2167 3343 1537
Size: 275 355 1176 4090
Fragments arranged by size:
4090 1176 355 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2690 3826 1353
Size: 1337 1136 3423
Fragments arranged by size:
3423 1337 1136
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2039 2416 4483 4857 5003 387
Size: 1064 138 450 377 2067 374 146 1280
Fragments arranged by size:
2067 1280 1064 450 377 374 146 138
EcoICRI GAG'CTC
Cuts at: 2329 3059 2329
Size: 730 5166
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2312 3959 4556 5343 5611 1239
Size: 393 680 1647 597 787 268 1524
Fragments arranged by size:
1647 1524 787 680 597 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2890 2890
Size: 5896
EcoRI G'AATT_C
Cuts at: 3205 3583 3205
Size: 378 5518
FspI TGC'GCA
Cuts at: 4402 4402
Size: 5896
HaeII r_GCGC'y
Cuts at: 1678 2157 2708 5043 1678
Size: 479 551 2335 2531
Fragments arranged by size:
2531 2335 551 479
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2076 2108 2140 2445 2477
Size: 32 135 32 504 32 32 305 32
Cuts at: 2477 2703 2735 3008 3040 4537 4569 4611 4643
Size: 226 32 273 32 1497 32 42 32
Cuts at: 4643 1373
Size: 2626
Fragments arranged by size:
2626 1497 504 305 273 226 135 42
32 32 32 32 32 32 32 32
32
HincII GTy'rAC
Cuts at: 669 1657 1938 3240 3486 669
Size: 988 281 1302 246 3079
Fragments arranged by size:
3079 1302 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5896
HpaI GTT'AAC
Cuts at: 3486 3486
Size: 5896
KasI G'GCGC_C
Cuts at: 2153 2153
Size: 5896
KpnI G_GTAC'C
Cuts at: 3221 3221
Size: 5896
MfeI C'AATT_G
Cuts at: 3495 3495
Size: 5896
MluI A'CGCG_T
Cuts at: 5772 5772
Size: 5896
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5010 5194 1305
Size: 3705 184 2007
Fragments arranged by size:
3705 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2147 10
Size: 54 1425 343 315 3759
Fragments arranged by size:
3759 1425 343 315 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3119 3854 4213 4372 518
Size: 648 1953 735 359 159 2042
Fragments arranged by size:
2042 1953 735 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2409 2599 3054 3312 3989 5137 5382 1606
Size: 803 190 455 258 677 1148 245 2120
Fragments arranged by size:
2120 1148 803 677 455 258 245 190
NaeI GCC'GGC
Cuts at: 2221 2221
Size: 5896
NarI GG'CG_CC
Cuts at: 2154 2154
Size: 5896
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4405
Fragments arranged by size:
4405 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5896
NgoMIV G'CCGG_C
Cuts at: 2219 2219
Size: 5896
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5896
NotI GC'GGCC_GC
Cuts at: 3343 3343
Size: 5896
NspI r_CATG'y
Cuts at: 1132 1229 2185 2815 3254 4799 1132
Size: 97 956 630 439 1545 2229
Fragments arranged by size:
2229 1545 956 630 439 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5896
PciI A'CATG_T
Cuts at: 4795 4795
Size: 5896
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5896
PmeI GTTT'AAAC
Cuts at: 3200 3200
Size: 5896
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3965 3997 5478 5510 3965
Size: 32 1481 32 4351
Fragments arranged by size:
4351 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2890 2890
Size: 5896
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5896
PsiI TTA'TAA
Cuts at: 3466 3466
Size: 5896
PspXI vC'TCGA_Gb
Cuts at: 2318 2318
Size: 5896
PstI C_TGCA'G
Cuts at: 830 3248 830
Size: 2418 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5896
PvuII CAG'CTG
Cuts at: 2599 2599
Size: 5896
SacI G_AGCT'C
Cuts at: 2331 3061 2331
Size: 730 5166
SalI G'TCGA_C
Cuts at: 1936 3238 1936
Size: 1302 4594
SapI GCTCTTCn'nnn_
Cuts at: 2690 2690
Size: 5896
SbfI CC_TGCA'GG
Cuts at: 3248 3248
Size: 5896
ScaI AGT'ACT
Cuts at: 4144 5856 4144
Size: 1712 4184
SfcI C'TryA_G
Cuts at: 826 1045 2377 3117 3244 4379 5060 5251 826
Size: 219 1332 740 127 1135 681 191 1471
Fragments arranged by size:
1471 1332 1135 740 681 219 191 127
SfiI GGCCn_nnn'nGGCC
Cuts at: 2144 2144
Size: 5896
SfoI GGC'GCC
Cuts at: 2155 2155
Size: 5896
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5896
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5896
SmaI CCC'GGG
Cuts at: 1872 3223 1872
Size: 1351 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2318 2894 4000 4901 5163 820
Size: 197 926 375 576 1106 901 262 1553
Fragments arranged by size:
1553 1106 926 901 576 375 262 197
SnaBI TAC'GTA
Cuts at: 493 3687 493
Size: 3194 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5896
SphI G_CATG'C
Cuts at: 1132 2185 2815 3254 1132
Size: 1053 630 439 3774
Fragments arranged by size:
3774 1053 630 439
SspI AAT'ATT
Cuts at: 5 52 3820 5
Size: 47 3768 2081
Fragments arranged by size:
3768 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5896
StyI C'CwwG_G
Cuts at: 513 1065 2004 2095 2343 2416 2886 3335 513
Size: 552 939 91 248 73 470 449 3074
Fragments arranged by size:
3074 939 552 470 449 248 91 73
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3923 3940 4093 4278 4723 325
Size: 1255 2343 17 153 185 445 1498
Fragments arranged by size:
2343 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2547 4142 5854
Size: 274 80 33 51 1443 570 1595 1712
Cuts at: 5854 96
Size: 138
Fragments arranged by size:
1712 1595 1443 570 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2063 2095 2258 2290 3013 3045 5856 5888 2063
Size: 32 163 32 723 32 2811 32 2071
Fragments arranged by size:
2811 2071 723 163 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2459 2720 1316
Size: 1143 261 4492
Fragments arranged by size:
4492 1143 261
XbaI T'CTAG_A
Cuts at: 3232 3232
Size: 5896
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2011 2011
Size: 5896
XhoI C'TCGA_G
Cuts at: 1943 2318 1943
Size: 375 5521
XmaI C'CCGG_G
Cuts at: 1870 3221 1870
Size: 1351 4545
XmnI GAAnn'nnTTC
Cuts at: 4025 4025
Size: 5896
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3704 276
Size: 53 83 186 936 2170 2468
Fragments arranged by size:
2468 2170 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NdeI NgoMIV NheI NotI NspI PasI PciI PflMI
PmeI PpiI PpuMI PshAI PsiI PspXI PstI PvuI
PvuII SacI SalI SapI SbfI ScaI SfcI SfiI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI ApaI AscI AvrII BaeI BfrBI BplI BsiWI
BsmBI BstXI BstZ17I Bsu36I DraIII EcoNI EcoRV FalI
FseI FspAI NruI NsiI PacI PfoI PmlI PspOMI
PsrI RsrII SacII SanDI SexAI SrfI SwaI