################################## # NO CUT ENZYMES ( 147 enzymes) # ################################## AatII Acc65I AccII AccIII AclI AcyI AgeI AhdI Alw44I AlwNI AocI Aor51HI ApaLI AscI Asp718I AspEI AspHI AspI AviII AvrII BalI BamHI BanI BbeI BbrPI BbsI BbvCI BcgI BclI BcnI BglI BglII BlnI BpuAI BsaAI BsaHI BsaI BsaWI BseAI BsiEI BsiHKAI BsiWI BsmI BspDI BspEI BspHI BspMI BsrBI BsrFI BssHII BssSI Bst1107I BstBI BstUI BstXI BstZ17I Bsu36I Cac8I CfoI Cfr10I ClaI CpoI DraI DrdI EaeI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoT22I FauI FbaI FseI FspI HaeII HapII HgaI HhaI Hin1I HinP1I HindIII HpaI HpaII KasI KpnI Ksp632I KspI MfeI MluI MluNI MroI MscI MspA1I MspI MunI MvnI NaeI NarI NciI NgoMIV NheI NotI NruI NsiI NspV PacI PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI Psp1406I PvuI PvuII RcaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspI StuI SwaI Tth111I Van91I XcmI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 48 enzymes) # ################################### AciI 1171 CCGC CCGC(-3/-1) AflII 982 CTTAAG C^TTAAG ApaI 1348 GGGCCC GGGCC^C AseI 934 ATTAAT AT^TAAT AsnI 934 ATTAAT AT^TAAT Asp700I 254 GAATGTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AvaI 823 CTCGAG C^[CT]CG[GA]G BbvI 419 GCAGC GCAGC(8/12) BfrI 982 CTTAAG C^TTAAG BlpI 472 GCTCAGC GC^T[ACGT]AGC Bpu1102I 472 GCTCAGC GC^T[ACGT]AGC BsgI 1140 CTGCAC GTGCAG(16/14) BsiYI 41 CCACTGTTGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BslI 41 CCACTGTTGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BsmBI 503 GAGACG CGTCTC(1/5) BsmFI 850 GTCCC GGGAC(10/14) BsoBI 823 CTCGAG C^[CT]CG[GA]G Bsp1407I 327 TGTACA T^GTACA BspLU11I 802 ACATGT A^CATGT BsrDI 904 GCAATG GCAATG(2/0) BsrGI 327 TGTACA T^GTACA BstAPI 206 GCATAACATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstYI 1512 AGATCC [GA]^GATC[CT] CelII 472 GCTCAGC GC^T[ACGT]AGC DraII 1348 GGGCCCC [GA]G^G[ACGT]CC[CT] DsaI 87 CCATGG C^C[GA][CT]GG EcoO109I 1348 GGGCCCC [GA]G^G[ACGT]CC[CT] EcoRI 1612 GAATTC G^AATTC EcoRV 1644 GATATC GAT^ATC EcoT14I 87 CCATGG C^C[AT][AT]GG Fnu4HI 419 GCAGC GC^[ACGT]GC HincII 1413 GTCAAC GT[CT]^[GA]AC HindII 1413 GTCAAC GT[CT]^[GA]AC ItaI 419 GCAGC GC^[ACGT]GC MflI 1512 AGATCC [GA]^GATC[CT] MwoI 206 GCATAACATGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC NcoI 87 CCATGG C^CATGG NdeI 414 CATATG CA^TATG PaeR7I 823 CTCGAG C^TCGAG PshBI 934 ATTAAT AT^TAAT PstI 522 CTGCAG CTGCA^G SspBI 327 TGTACA T^GTACA StyI 87 CCATGG C^C[AT][AT]GG TseI 419 GCAGC G^C[AT]GC XbaI 1057 TCTAGA T^CTAGA XhoI 823 CTCGAG C^TCGAG XhoII 1512 AGATCC [GA]^GATC[CT] XmnI 254 GAATGTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 28 enzymes) # ################################### AccI 1135 GTAGAC GT^[AC][GT]AC 1332 GTCTAC AflIII 506 ACGTGT A^C[GA][CT]GT 802 ACATGT AlwI 1513 GATCC GGATC(4/5) 547 GGATC AvaII 401 GGTCC G^G[AT]CC 625 GGTCC BanII 1348 GGGCCC G[GA]GC[CT]^C 1455 GGGCTC BfaI 1058 CTAG C^TAG 1510 CTAG BmyI 1348 GGGCCC G[AGT]GC[ACT]^C 1455 GGGCTC BpmI 1406 CTCCAG CTGGAG(16/14) 1450 CTCCAG BsaBI 706 GATTGCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 1304 GATGGCAATC BseRI 407 CTCCTC GAGGAG(10/8) 632 CTCCTC Bsp1286I 1348 GGGCCC G[AGT]GC[ACT]^C 1455 GGGCTC BsrI 1434 CCAGT ACTGG(1/-1) 1617 CCAGT BssKI 1408 CCAGG ^CC[ACGT]GG 1452 CCAGG BstEII 230 GGTCACC G^GT[ACGT]ACC 581 GGTCACC BstNI 1408 CCAGG CC^[AT]GG 1452 CCAGG BstPI 230 GGTCACC G^GT[ACGT]ACC 581 GGTCACC DraIII 62 CACTTTGTG CAC[ACGT][ACGT][ACGT]^GTG 1319 CACGCTGTG EcoO65I 230 GGTCACC G^GT[ACGT]ACC 581 GGTCACC EcoRII 1408 CCAGG ^CC[AT]GG 1452 CCAGG HaeIII 734 GGCC GG^CC 1349 GGCC MaeI 1058 CTAG C^TAG 1510 CTAG MamI 706 GATTGCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 1304 GATGGCAATC MslI 239 CACCGAAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 1499 CACCAAGATG MvaI 1408 CCAGG CC^[AT]GG 1452 CCAGG NlaIV 1348 GGGCCC GG[ACGT]^[ACGT]CC 1349 GGCCCC ScrFI 1408 CCAGG CC^[ACGT]GG 1452 CCAGG SfcI 137 CTACAG C^T[GA][CT]AG 522 CTGCAG TfiI 292 GAATC G^A[AT]TC 1125 GAATC ######################################### # 3 OR MORE CUT ENZYMES ( 35 enzymes) # ######################################### AcsI AfaI AluI ApoI BciVI BsaJI BsmAI BstF5I Cfr13I DdeI DpnII FokI HinfI HphI MaeII MaeIII MboI MboII MnlI MseI NdeII NlaIII NspI PleI RsaI Sau3AI Sau96I SfaNI SmlI TaqI Tru9I Tsp45I Tsp509I TspRI TthHB8I