(Circular) (Six-base) MAPSORT of: pFN21AE1991.seq Check: 9083 from: 1 to: 6412 pFN21AE1991 6412 bp With 182 enzymes: SgfI * August 16, 2010 14:53 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4222 278 Size: 53 83 186 936 2686 2468 Fragments arranged by size: 2686 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3141 3338 3755 1937 Size: 1204 197 417 4594 Fragments arranged by size: 4594 1204 417 197 Acc65I G'GTAC_C Cuts at: 3733 3733 Size: 6412 AclI AA'CG_TT Cuts at: 1563 4539 4912 1563 Size: 2976 373 3063 Fragments arranged by size: 3063 2976 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2286 3076 3156 3555 4475 5859 6127 1632 Size: 654 790 80 399 920 1384 268 1917 Fragments arranged by size: 1917 1384 920 790 654 399 268 80 AflII C'TTAA_G Cuts at: 820 1017 2987 820 Size: 197 1970 4245 Fragments arranged by size: 4245 1970 197 AflIII A'CryG_T Cuts at: 1203 2511 2807 5311 6288 1203 Size: 1308 296 2504 977 1327 Fragments arranged by size: 2504 1327 1308 977 296 AgeI A'CCGG_T Cuts at: 5243 5243 Size: 6412 AhdI GACnn_n'nnGTC Cuts at: 5141 5141 Size: 6412 AleI CACnn'nnGTG Cuts at: 1166 2248 1166 Size: 1082 5330 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6412 AlwNI CAG_nnn'CTG Cuts at: 4155 5727 4155 Size: 1572 4840 ApaI G_GGCC'C Cuts at: 3357 3357 Size: 6412 ApaLI G'TGCA_C Cuts at: 4469 5625 4469 Size: 1156 5256 ApoI r'AATT_y Cuts at: 1493 2519 3047 3617 3629 3689 3721 3947 4099 Size: 1026 528 570 12 60 32 226 152 Cuts at: 4099 6208 1493 Size: 2109 1697 Fragments arranged by size: 2109 1697 1026 570 528 226 152 60 32 12 AseI AT'TA_AT Cuts at: 160 1032 2940 4966 160 Size: 872 1908 2026 1606 Fragments arranged by size: 2026 1908 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6412 AvaI C'yCGr_G Cuts at: 1870 1943 2828 3737 1870 Size: 73 885 909 4545 Fragments arranged by size: 4545 909 885 73 BamHI G'GATC_C Cuts at: 3742 4109 3742 Size: 367 6045 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3733 5188 618 Size: 325 217 627 1946 1455 1842 Fragments arranged by size: 1946 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 3357 3464 3731 1391 Size: 1966 107 267 4072 Fragments arranged by size: 4072 1966 267 107 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5441 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4603 4637 1903 Size: 34 2666 34 3678 Fragments arranged by size: 3678 2666 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2053 2348 2807 4304 5514 2053 Size: 295 459 1497 1210 2951 Fragments arranged by size: 2951 1497 1210 459 295 BclI T'GATC_A Cuts at: 1548 1548 Size: 6412 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5023 136 Size: 107 122 71 631 605 3351 1525 Fragments arranged by size: 3351 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6407 6407 Size: 6412 BlpI GC'TnA_GC Cuts at: 2478 3829 2478 Size: 1351 5061 Bme1580I G_kGCm'C Cuts at: 1790 3357 4473 5629 1790 Size: 1567 1116 1156 2573 Fragments arranged by size: 2573 1567 1156 1116 BmgBI CAC'GTC Cuts at: 1318 2512 1318 Size: 1194 5218 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4143 5101 442 Size: 416 53 83 399 24 2726 958 1753 Fragments arranged by size: 2726 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6412 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3396 3440 5072 1239 Size: 2157 44 1632 2579 Fragments arranged by size: 2579 2157 1632 44 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3014 4537 5402 5700 3014 Size: 1523 865 298 3726 Fragments arranged by size: 3726 1523 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5075 882 Size: 617 3576 2219 Fragments arranged by size: 3576 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4203 493 Size: 711 2999 2702 Fragments arranged by size: 2999 2702 711 BsaBI GATnn'nnATC Cuts at: 2715 3313 6406 2715 Size: 598 3093 2721 Fragments arranged by size: 3093 2721 598 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4219 4601 5236 275 Size: 53 83 186 936 2686 382 635 1451 Fragments arranged by size: 2686 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4845 5243 5517 5664 6075 4845 Size: 398 274 147 411 5182 Fragments arranged by size: 5182 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2865 2895 3225 3255 1178 Size: 30 165 30 1462 30 330 30 4335 Fragments arranged by size: 4335 1462 330 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2403 2628 1186 Size: 1217 225 4970 Fragments arranged by size: 4970 1217 225 BseYI C'CCAG_C Cuts at: 5615 6111 5615 Size: 496 5916 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3130 1163 Size: 573 1394 4445 Fragments arranged by size: 4445 1394 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3862 4623 5651 1540 Size: 275 186 1861 761 1028 2301 Fragments arranged by size: 2301 1861 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4473 4558 5629 4473 Size: 85 1071 5256 Fragments arranged by size: 5256 1071 85 BsmI GAATG_Cn' Cuts at: 3923 4016 3923 Size: 93 6319 BsmBI CGTCTCn'nnnn_ Cuts at: 2502 2502 Size: 6412 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3357 3464 3731 4473 4558 5629 1391 Size: 399 1567 107 267 742 85 1071 2174 Fragments arranged by size: 2174 1567 1071 742 399 267 107 85 BspEI T'CCGG_A Cuts at: 6075 6075 Size: 6412 BspHI T'CATG_A Cuts at: 4301 6031 4301 Size: 1730 4682 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3767 844 Size: 2923 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4299 1394 Size: 429 2476 3507 Fragments arranged by size: 3507 2476 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2916 4907 5081 65 Size: 1183 1668 1991 174 1396 Fragments arranged by size: 1991 1668 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5056 5243 1700 Size: 110 3246 187 2869 Fragments arranged by size: 3246 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 2332 96 Size: 2236 4176 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6412 BssSI C'ACGA_G Cuts at: 4472 5484 4472 Size: 1012 5400 BstAPI GCAn_nnn'nTGC Cuts at: 794 2217 794 Size: 1423 4989 BstBI TT'CG_AA Cuts at: 5216 5216 Size: 6412 BstEII G'GTnAC_C Cuts at: 1184 2235 2586 1184 Size: 1051 351 5010 Fragments arranged by size: 5010 1051 351 BstYI r'GATC_y Cuts at: 1972 3517 3742 4109 4494 4511 5963 6049 6061 Size: 1545 225 367 385 17 1452 86 12 Cuts at: 6061 6407 1972 Size: 346 1977 Fragments arranged by size: 1977 1545 1452 385 367 346 225 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2092 513 Size: 552 1027 4833 Fragments arranged by size: 4833 1027 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3659 487 Size: 683 56 2433 3240 Fragments arranged by size: 3240 2433 683 56 BtsI GCAGTG_nn' Cuts at: 3928 4722 4742 3928 Size: 794 20 5598 Fragments arranged by size: 5598 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6412 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2564 2599 2773 2808 529 Size: 35 2000 35 174 35 4133 Fragments arranged by size: 4133 2000 174 35 35 35 DraI TTT'AAA Cuts at: 3716 3716 Size: 6412 DraIII CAC_nnn'GTG Cuts at: 2072 3329 2072 Size: 1257 5155 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5419 809 Size: 507 4103 1802 Fragments arranged by size: 4103 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3859 4748 6377 Size: 54 1425 50 275 18 2029 889 1629 Cuts at: 6377 8 Size: 43 Fragments arranged by size: 2029 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3859 1537 Size: 275 2047 4090 Fragments arranged by size: 4090 2047 275 EarI CTCTTCn'nnn_ Cuts at: 1353 4342 1353 Size: 2989 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4999 5373 5519 387 Size: 1064 138 3410 374 146 1280 Fragments arranged by size: 3410 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2286 3076 3156 3396 3440 3555 4475 Size: 393 654 790 80 240 44 115 920 Cuts at: 4475 5072 5859 6127 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 920 790 787 654 597 393 268 240 115 80 44 EcoO109I rG'GnC_Cy Cuts at: 3354 3354 Size: 6412 EcoRI G'AATT_C Cuts at: 3617 3721 4099 3617 Size: 104 378 5930 Fragments arranged by size: 5930 378 104 EcoRV GAT'ATC Cuts at: 3651 3651 Size: 6412 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2084 2084 Size: 6412 FspI TGC'GCA Cuts at: 4918 4918 Size: 6412 HaeII r_GCGC'y Cuts at: 1678 5559 1678 Size: 3881 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2865 2897 3371 3403 5053 Size: 32 135 32 1293 32 474 32 1650 Cuts at: 5053 5085 5127 5159 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1650 1293 474 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3420 3756 4002 669 Size: 988 281 1482 336 246 3079 Fragments arranged by size: 3079 1482 988 336 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6412 HpaI GTT'AAC Cuts at: 4002 4002 Size: 6412 KpnI G_GTAC'C Cuts at: 3737 3737 Size: 6412 MfeI C'AATT_G Cuts at: 4011 4011 Size: 6412 MluI A'CGCG_T Cuts at: 6288 6288 Size: 6412 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2485 2530 2624 5526 5710 1305 Size: 1180 45 94 2902 184 2007 Fragments arranged by size: 2902 2007 1180 184 94 45 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4590 Fragments arranged by size: 4590 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2248 3508 4370 4729 4888 518 Size: 648 1082 1260 862 359 159 2042 Fragments arranged by size: 2042 1260 1082 862 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3828 4505 5653 5898 1606 Size: 2222 677 1148 245 2120 Fragments arranged by size: 2222 2120 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2092 513 Size: 552 1027 4833 Fragments arranged by size: 4833 1027 552 NdeI CA'TA_TG Cuts at: 387 2420 387 Size: 2033 4379 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6412 NotI GC'GGCC_GC Cuts at: 3859 3859 Size: 6412 NspI r_CATG'y Cuts at: 1132 1229 2220 2607 2811 3480 3770 5315 1132 Size: 97 991 387 204 669 290 1545 2229 Fragments arranged by size: 2229 1545 991 669 387 290 204 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6412 PciI A'CATG_T Cuts at: 2807 5311 2807 Size: 2504 3908 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6412 PmeI GTTT'AAAC Cuts at: 3716 3716 Size: 6412 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4481 4513 5994 6026 4481 Size: 32 1481 32 4867 Fragments arranged by size: 4867 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6412 PsiI TTA'TAA Cuts at: 2121 2543 2729 3982 2121 Size: 422 186 1253 4551 Fragments arranged by size: 4551 1253 422 186 PspOMI G'GGCC_C Cuts at: 3353 3353 Size: 6412 PspXI vC'TCGA_Gb Cuts at: 2828 2828 Size: 6412 PstI C_TGCA'G Cuts at: 830 2531 3764 830 Size: 1701 1233 3478 Fragments arranged by size: 3478 1701 1233 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6412 SalI G'TCGA_C Cuts at: 1936 3754 1936 Size: 1818 4594 SbfI CC_TGCA'GG Cuts at: 3764 3764 Size: 6412 ScaI AGT'ACT Cuts at: 4660 6372 4660 Size: 1712 4700 SfcI C'TryA_G Cuts at: 826 1045 2142 2527 3760 4895 5576 5767 826 Size: 219 1097 385 1233 1135 681 191 1471 Fragments arranged by size: 1471 1233 1135 1097 681 385 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6412 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6412 SmaI CCC'GGG Cuts at: 1872 3739 1872 Size: 1867 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2828 2987 3029 4516 5417 5679 Size: 197 926 885 159 42 1487 901 262 Cuts at: 5679 820 Size: 1553 Fragments arranged by size: 1553 1487 926 901 885 262 197 159 42 SnaBI TAC'GTA Cuts at: 493 4203 493 Size: 3710 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6412 SphI G_CATG'C Cuts at: 1132 3770 1132 Size: 2638 3774 SspI AAT'ATT Cuts at: 5 52 4336 5 Size: 47 4284 2081 Fragments arranged by size: 4284 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6412 StyI C'CwwG_G Cuts at: 513 1065 2092 3851 513 Size: 552 1027 1759 3074 Fragments arranged by size: 3074 1759 1027 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2571 4439 4456 4609 4794 5239 325 Size: 1255 991 1868 17 153 185 445 1498 Fragments arranged by size: 1868 1498 1255 991 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2332 4658 6370 Size: 274 80 33 51 1443 355 2326 1712 Cuts at: 6370 96 Size: 138 Fragments arranged by size: 2326 1712 1443 355 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6372 6404 6372 Size: 32 6380 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6412 XbaI T'CTAG_A Cuts at: 3062 3748 3062 Size: 686 5726 XhoI C'TCGA_G Cuts at: 1943 2828 1943 Size: 885 5527 XmaI C'CCGG_G Cuts at: 1870 3737 1870 Size: 1867 4545 XmnI GAAnn'nnTTC Cuts at: 2263 4541 2263 Size: 2278 4134 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4220 276 Size: 53 83 186 936 2686 2468 Fragments arranged by size: 2686 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI PspXI PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BstXI BstZ17I Bsu36I EcoICRI EcoNI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI