################################## # NO CUT ENZYMES ( 130 enzymes) # ################################## AatII Acc65I AccIII AclI AcyI AflII AflIII AgeI AhdI AocI ApaI AscI AseI AsnI Asp718I AspEI AspI AvrII BanI BanII BbeI BbrPI BcgI BciVI BclI BfrI BglI BlnI BpmI BsaAI BsaBI BsaHI BsaI BsaWI BseAI BsiEI BsiWI BsmI BspDI BspEI BspHI BspLU11I BsrBI BsrDI BsrFI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstZ17I Bsu36I Cfr10I ClaI CpoI DraI DraIII DrdI EagI Eam1105I EclXI Eco52I Eco81I EcoO65I EcoRI EcoT22I FauI FbaI FseI Hin1I HindIII HpaI KasI KpnI KspI MamI MfeI MluI MroI MslI MunI NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RcaI RsrII SacI SacII SalI SapI SbfI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I StuI SwaI Tth111I Van91I XcmI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 46 enzymes) # ################################### AccI 1282 GTAGAC GT^[AC][GT]AC AccII 1321 CGCG CG^CG Aor51HI 331 AGCGCT AGC^GCT Asp700I 241 GAAAATATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AvaI 662 CCCGAG C^[CT]CG[GA]G AviII 839 TGCGCA TGC^GCA BalI 1369 TGGCCA TGG^CCA BbvCI 646 GCTGAGG CCTCAGC(-5/-2) BcnI 986 CCCGG CC^[GC]GG BfaI 601 CTAG C^TAG BlpI 12 GCTTAGC GC^T[ACGT]AGC Bpu1102I 12 GCTTAGC GC^T[ACGT]AGC BseRI 630 GAGGAG GAGGAG(10/8) BsmAI 65 GAGAC GTCTC(1/5) BsmBI 65 GAGACG CGTCTC(1/5) BsoBI 662 CCCGAG C^[CT]CG[GA]G Bsp1407I 492 TGTACA T^GTACA BspMI 804 ACCTGC ACCTGC(4/8) BsrGI 492 TGTACA T^GTACA BstUI 1321 CGCG CG^CG BstXI 560 CCAAAATTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Cac8I 786 GCGTGC GC[ACGT]^[ACGT]GC CelII 12 GCTTAGC GC^T[ACGT]AGC EaeI 1369 TGGCCA [CT]^GGCC[GA] EarI 427 GAAGAG CTCTTC(1/4) Eco47III 331 AGCGCT AGC^GCT EcoNI 1137 CCTTCTACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoRV 287 GATATC GAT^ATC FspI 839 TGCGCA TGC^GCA HaeII 331 AGCGCT [GA]GCGC^[CT] HapII 987 CCGG C^CGG HpaII 987 CCGG C^CGG Ksp632I 427 GAAGAG CTCTTC(1/4) MaeI 601 CTAG C^TAG MluNI 1369 TGGCCA TGG^CCA MscI 1369 TGGCCA TGG^CCA MspA1I 860 CAGCTG C[AC]G^C[GT]G MspI 987 CCGG C^CGG MvnI 1321 CGCG CG^CG NciI 986 CCCGG CC^[GC]GG PvuII 860 CAGCTG CAG^CTG ScaI 158 AGTACT AGT^ACT SspBI 492 TGTACA T^GTACA SspI 244 AATATT AAT^ATT XbaI 600 TCTAGA T^CTAGA XmnI 241 GAAAATATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 27 enzymes) # ################################### AciI 1320 CCGC CCGC(-3/-1) 4 GCGG AcsI 729 GAATTT [GA]^AATT[CT] 1153 AAATTT Alw44I 199 GTGCAC G^TGCAC 788 GTGCAC ApaLI 199 GTGCAC G^TGCAC 788 GTGCAC ApoI 729 GAATTT [GA]^AATT[CT] 1153 AAATTT AspHI 199 GTGCAC G[AT]GC[AT]^C 788 GTGCAC BamHI 828 GGATCC G^GATCC 1455 GGATCC BglII 276 AGATCT A^GATCT 580 AGATCT BsgI 230 GTGCAG GTGCAG(16/14) 1339 GTGCAG BsiHKAI 199 GTGCAC G[AT]GC[AT]^C 788 GTGCAC BstNI 92 CCTGG CC^[AT]GG 1240 CCAGG DraII 1145 AGGACCT [GA]G^G[ACGT]CC[CT] 1344 GGGTCCT DsaI 945 CCATGG C^C[GA][CT]GG 1372 CCATGG EcoO109I 1145 AGGACCT [GA]G^G[ACGT]CC[CT] 1344 GGGTCCT EcoRII 92 CCTGG ^CC[AT]GG 1240 CCAGG EcoT14I 945 CCATGG C^C[AT][AT]GG 1372 CCATGG HgaI 17 GCGTC GACGC(5/10) 304 GCGTC HincII 306 GTCAAC GT[CT]^[GA]AC 1190 GTCAAC HindII 306 GTCAAC GT[CT]^[GA]AC 1190 GTCAAC MvaI 92 CCTGG CC^[AT]GG 1240 CCAGG NcoI 945 CCATGG C^CATGG 1372 CCATGG PleI 993 GACTC GAGTC(4/5) 999 GACTC PpuMI 1145 AGGACCT [GA]G^G[AT]CC[CT] 1344 GGGTCCT StyI 945 CCATGG C^C[AT][AT]GG 1372 CCATGG TaqI 270 TCGA T^CGA 1071 TCGA Tsp45I 476 GTCAC ^GT[GC]AC 658 GTGAC TthHB8I 270 TCGA T^CGA 1071 TCGA ######################################### # 3 OR MORE CUT ENZYMES ( 55 enzymes) # ######################################### AfaI AluI AlwI AlwNI AvaII BbsI BbvI BmyI BpuAI BsaJI BsiYI BslI BsmFI Bsp1286I BsrI BssKI BstF5I BstYI CfoI Cfr13I DdeI DpnII Fnu4HI FokI HaeIII HhaI HinP1I HinfI HphI ItaI MaeII MaeIII MboI MboII MflI MnlI MseI MwoI NdeII NlaIII NlaIV PstI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI TfiI Tru9I TseI Tsp509I TspRI XhoII