(Circular) (Six-base) MAPSORT of: pFN21AE2190.seq Check: 8734 from: 1 to: 6298 pFN21AE2190 6298 bp With 182 enzymes: SgfI * August 16, 2010 14:44 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4108 278 Size: 53 83 186 936 2572 2468 Fragments arranged by size: 2572 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3288 3641 1937 Size: 1351 353 4594 Fragments arranged by size: 4594 1351 353 Acc65I G'GTAC_C Cuts at: 3619 3619 Size: 6298 AclI AA'CG_TT Cuts at: 1563 4425 4798 1563 Size: 2862 373 3063 Fragments arranged by size: 3063 2862 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2342 2547 3458 4361 5745 6013 1632 Size: 710 205 911 903 1384 268 1917 Fragments arranged by size: 1917 1384 911 903 710 268 205 AfeI AGC'GCT Cuts at: 2338 2338 Size: 6298 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6101 AflIII A'CryG_T Cuts at: 1203 5197 6174 1203 Size: 3994 977 1327 Fragments arranged by size: 3994 1327 977 AgeI A'CCGG_T Cuts at: 5129 5129 Size: 6298 AhdI GACnn_n'nnGTC Cuts at: 5027 5027 Size: 6298 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6298 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3369 1184 Size: 2185 4113 AlwNI CAG_nnn'CTG Cuts at: 2243 2828 3154 4041 5613 2243 Size: 585 326 887 1572 2928 Fragments arranged by size: 2928 1572 887 585 326 ApaLI G'TGCA_C Cuts at: 2204 2793 4355 5511 2204 Size: 589 1562 1156 2991 Fragments arranged by size: 2991 1562 1156 589 ApoI r'AATT_y Cuts at: 1493 2734 3158 3607 3833 3985 6094 1493 Size: 1241 424 449 226 152 2109 1697 Fragments arranged by size: 2109 1697 1241 449 424 226 152 AseI AT'TA_AT Cuts at: 160 1032 4852 160 Size: 872 3820 1606 Fragments arranged by size: 3820 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6298 AvaI C'yCGr_G Cuts at: 1870 1943 2667 3623 1870 Size: 73 724 956 4545 Fragments arranged by size: 4545 956 724 73 BamHI G'GATC_C Cuts at: 2833 3460 3628 3995 2833 Size: 627 168 367 5136 Fragments arranged by size: 5136 627 367 168 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3619 5074 618 Size: 325 217 627 1832 1455 1842 Fragments arranged by size: 1842 1832 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 3617 1391 Size: 2226 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2120 2272 2348 3002 928 Size: 971 221 152 76 654 4224 Fragments arranged by size: 4224 971 654 221 152 76 BbvCI CC'TCA_GC Cuts at: 2652 2652 Size: 6298 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4489 4523 1903 Size: 34 2552 34 3678 Fragments arranged by size: 3678 2552 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4190 5400 4190 Size: 1210 5088 BclI T'GATC_A Cuts at: 1548 1548 Size: 6298 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4909 136 Size: 107 122 71 631 605 3237 1525 Fragments arranged by size: 3237 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2281 2585 6293 2281 Size: 304 3708 2286 Fragments arranged by size: 3708 2286 304 BlpI GC'TnA_GC Cuts at: 2018 3715 2018 Size: 1697 4601 Bme1580I G_kGCm'C Cuts at: 1790 2208 2797 3254 4359 5515 1790 Size: 418 589 457 1105 1156 2573 Fragments arranged by size: 2573 1156 1105 589 457 418 BmgBI CAC'GTC Cuts at: 1318 2014 2234 1318 Size: 696 220 5382 Fragments arranged by size: 5382 696 220 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3328 4029 4987 Size: 416 53 83 399 24 1911 701 958 Cuts at: 4987 442 Size: 1753 Fragments arranged by size: 1911 1753 958 701 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6298 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4958 1239 Size: 3719 2579 Bpu10I CC'TnA_GC Cuts at: 2652 2652 Size: 6298 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2355 3024 3297 3610 4423 5288 5586 2355 Size: 669 273 313 813 865 298 3067 Fragments arranged by size: 3067 865 813 669 313 298 273 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4961 882 Size: 617 3462 2219 Fragments arranged by size: 3462 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4089 493 Size: 711 2885 2702 Fragments arranged by size: 2885 2702 711 BsaBI GATnn'nnATC Cuts at: 6292 6292 Size: 6298 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4105 4487 5122 275 Size: 53 83 186 936 2572 382 635 1451 Fragments arranged by size: 2572 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4731 5129 5403 5550 5961 4731 Size: 398 274 147 411 5068 Fragments arranged by size: 5068 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2061 2091 2989 3019 1178 Size: 30 165 30 658 30 898 30 4457 Fragments arranged by size: 4457 898 658 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2650 1186 Size: 1464 4834 BseYI C'CCAG_C Cuts at: 2863 3015 5501 5997 2863 Size: 152 2486 496 3164 Fragments arranged by size: 3164 2486 496 152 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2256 3365 1163 Size: 573 520 1109 4096 Fragments arranged by size: 4096 1109 573 520 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3748 4509 5537 1540 Size: 275 186 1747 761 1028 2301 Fragments arranged by size: 2301 1747 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2208 2797 4359 4444 5515 2208 Size: 589 1562 85 1071 2991 Fragments arranged by size: 2991 1562 1071 589 85 BsmI GAATG_Cn' Cuts at: 3809 3902 3809 Size: 93 6205 BsmBI CGTCTCn'nnnn_ Cuts at: 2064 2064 Size: 6298 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2208 2797 3254 3617 4359 4444 5515 Size: 399 418 589 457 363 742 85 1071 Cuts at: 5515 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 589 457 418 399 363 85 BspEI T'CCGG_A Cuts at: 5961 5961 Size: 6298 BspHI T'CATG_A Cuts at: 4187 5917 4187 Size: 1730 4568 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2818 3653 844 Size: 1974 835 3489 Fragments arranged by size: 3489 1974 835 BsrBI CCG'CTC Cuts at: 1394 1823 4185 1394 Size: 429 2362 3507 Fragments arranged by size: 3507 2362 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4793 4967 65 Size: 1183 3545 174 1396 Fragments arranged by size: 3545 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4942 5129 1700 Size: 110 3132 187 2869 Fragments arranged by size: 3132 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 2497 96 Size: 2401 3897 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6298 BssSI C'ACGA_G Cuts at: 4358 5370 4358 Size: 1012 5286 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6298 BstBI TT'CG_AA Cuts at: 5102 5102 Size: 6298 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6298 BstXI CCAn_nnnn'nTGG Cuts at: 2572 2572 Size: 6298 BstYI r'GATC_y Cuts at: 1972 2281 2585 2833 3071 3460 3628 3995 4380 Size: 309 304 248 238 389 168 367 385 Cuts at: 4380 4397 5849 5935 5947 6293 1972 Size: 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 389 385 367 346 309 304 248 238 168 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2950 3377 513 Size: 552 939 946 427 3434 Fragments arranged by size: 3434 946 939 552 427 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5559 Fragments arranged by size: 5559 683 56 BtsI GCAGTG_nn' Cuts at: 3252 3814 4608 4628 3252 Size: 562 794 20 4922 Fragments arranged by size: 4922 794 562 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6298 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6263 DraI TTT'AAA Cuts at: 3602 3602 Size: 6298 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5305 809 Size: 507 3989 1802 Fragments arranged by size: 3989 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3374 3745 4634 Size: 54 1425 50 275 18 1544 371 889 Cuts at: 4634 6263 8 Size: 1629 43 Fragments arranged by size: 1629 1544 1425 889 371 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3745 1537 Size: 275 1933 4090 Fragments arranged by size: 4090 1933 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2427 4228 1353 Size: 1074 1801 3423 Fragments arranged by size: 3423 1801 1074 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2024 4885 5259 5405 387 Size: 1064 138 435 2861 374 146 1280 Fragments arranged by size: 2861 1280 1064 435 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2342 2547 3458 4361 4958 5745 6013 Size: 393 710 205 911 903 597 787 268 Cuts at: 6013 1239 Size: 1524 Fragments arranged by size: 1524 911 903 787 710 597 393 268 205 EcoNI CCTnn'n_nnAGG Cuts at: 3146 3146 Size: 6298 EcoO109I rG'GnC_Cy Cuts at: 3151 3350 3151 Size: 199 6099 EcoRI G'AATT_C Cuts at: 3607 3985 3607 Size: 378 5920 EcoRV GAT'ATC Cuts at: 2294 2294 Size: 6298 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2303 2303 Size: 6298 FspI TGC'GCA Cuts at: 2846 4804 2846 Size: 1958 4340 HaeII r_GCGC'y Cuts at: 1678 2340 5445 1678 Size: 662 3105 2531 Fragments arranged by size: 3105 2531 662 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2577 2609 2661 2693 2969 Size: 32 135 32 1005 32 52 32 276 Cuts at: 2969 2989 3001 3021 4939 4971 5013 5045 1373 Size: 20 12 20 1918 32 42 32 2626 Fragments arranged by size: 2626 1918 1005 276 135 52 42 32 32 32 32 32 32 20 20 12 HincII GTy'rAC Cuts at: 669 1657 1938 2313 3197 3642 3888 669 Size: 988 281 375 884 445 246 3079 Fragments arranged by size: 3079 988 884 445 375 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6298 HpaI GTT'AAC Cuts at: 3888 3888 Size: 6298 KpnI G_GTAC'C Cuts at: 3623 3623 Size: 6298 MfeI C'AATT_G Cuts at: 3897 3897 Size: 6298 MluI A'CGCG_T Cuts at: 6174 6174 Size: 6298 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2254 5412 5596 1305 Size: 949 3158 184 2007 Fragments arranged by size: 3158 2007 949 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3376 10 Size: 54 1425 343 1544 2932 Fragments arranged by size: 2932 1544 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 4256 4615 4774 518 Size: 648 3090 359 159 2042 Fragments arranged by size: 3090 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2867 3714 4391 5539 5784 1606 Size: 1261 847 677 1148 245 2120 Fragments arranged by size: 2120 1261 1148 847 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 2950 3377 513 Size: 552 939 946 427 3434 Fragments arranged by size: 3434 946 939 552 427 NdeI CA'TA_TG Cuts at: 387 387 Size: 6298 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6298 NotI GC'GGCC_GC Cuts at: 3745 3745 Size: 6298 NspI r_CATG'y Cuts at: 1132 1229 3656 5201 1132 Size: 97 2427 1545 2229 Fragments arranged by size: 2427 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6298 PciI A'CATG_T Cuts at: 5197 5197 Size: 6298 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6298 PmeI GTTT'AAAC Cuts at: 3602 3602 Size: 6298 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4367 4399 5880 5912 4367 Size: 32 1481 32 4753 Fragments arranged by size: 4753 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3151 3350 3151 Size: 199 6099 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6298 PsiI TTA'TAA Cuts at: 3868 3868 Size: 6298 PstI C_TGCA'G Cuts at: 830 2389 3013 3103 3650 830 Size: 1559 624 90 547 3478 Fragments arranged by size: 3478 1559 624 547 90 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6298 PvuII CAG'CTG Cuts at: 2867 2867 Size: 6298 SalI G'TCGA_C Cuts at: 1936 3640 1936 Size: 1704 4594 SbfI CC_TGCA'GG Cuts at: 3650 3650 Size: 6298 ScaI AGT'ACT Cuts at: 2165 4546 6258 2165 Size: 2381 1712 2205 Fragments arranged by size: 2381 2205 1712 SfcI C'TryA_G Cuts at: 826 1045 2385 3009 3099 3146 3646 4781 5462 Size: 219 1340 624 90 47 500 1135 681 Cuts at: 5462 5653 826 Size: 191 1471 Fragments arranged by size: 1471 1340 1135 681 624 500 219 191 90 47 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6298 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6298 SmaI CCC'GGG Cuts at: 1872 3625 1872 Size: 1753 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2370 3039 3312 3589 4402 5303 Size: 197 926 427 669 273 277 813 901 Cuts at: 5303 5565 820 Size: 262 1553 Fragments arranged by size: 1553 926 901 813 669 427 277 273 262 197 SnaBI TAC'GTA Cuts at: 493 4089 493 Size: 3596 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6298 SphI G_CATG'C Cuts at: 1132 3656 1132 Size: 2524 3774 SspI AAT'ATT Cuts at: 5 52 2251 4222 5 Size: 47 2199 1971 2081 Fragments arranged by size: 2199 2081 1971 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6298 StyI C'CwwG_G Cuts at: 513 1065 2004 2950 3377 3737 513 Size: 552 939 946 427 360 3074 Fragments arranged by size: 3074 946 939 552 427 360 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3097 4325 4342 4495 4680 5125 325 Size: 1255 1517 1228 17 153 185 445 1498 Fragments arranged by size: 1517 1498 1255 1228 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2034 2163 2497 Size: 274 80 33 51 1443 57 129 334 Cuts at: 2497 4544 6256 96 Size: 2047 1712 138 Fragments arranged by size: 2047 1712 1443 334 274 138 129 80 57 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2061 2093 2420 2452 6258 6290 2061 Size: 32 327 32 3806 32 2069 Fragments arranged by size: 3806 2069 327 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6298 XbaI T'CTAG_A Cuts at: 2605 3634 2605 Size: 1029 5269 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6298 XmaI C'CCGG_G Cuts at: 1870 3623 1870 Size: 1753 4545 XmnI GAAnn'nnTTC Cuts at: 2250 4427 2250 Size: 2177 4121 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4106 276 Size: 53 83 186 936 2572 2468 Fragments arranged by size: 2572 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI ApaI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BstZ17I Bsu36I DraIII EcoICRI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI