(Linear) (Six-base) MAPSORT of: KIEE2190.seq Check: 6915 from: 1 to: 1593 KIEE2190.orf With 182 enzymes: SgfI * July 15, 2010 18:55 .. AccI GT'mk_AC Cuts at: 0 1283 1593 Size: 1283 310 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 337 542 1453 1593 Size: 337 205 911 140 Fragments arranged by size: 911 337 205 140 AfeI AGC'GCT Cuts at: 0 333 1593 Size: 333 1260 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1364 1593 Size: 1364 229 AlwNI CAG_nnn'CTG Cuts at: 0 238 823 1149 1593 Size: 238 585 326 444 Fragments arranged by size: 585 444 326 238 ApaLI G'TGCA_C Cuts at: 0 199 788 1593 Size: 199 589 805 Fragments arranged by size: 805 589 199 ApoI r'AATT_y Cuts at: 0 729 1153 1593 Size: 729 424 440 Fragments arranged by size: 729 440 424 AvaI C'yCGr_G Cuts at: 0 662 1593 Size: 662 931 BamHI G'GATC_C Cuts at: 0 828 1455 1593 Size: 828 627 138 Fragments arranged by size: 828 627 138 BbsI GAAGACnn'nnnn_ Cuts at: 0 115 267 343 997 1593 Size: 115 152 76 654 596 Fragments arranged by size: 654 596 152 115 76 BbvCI CC'TCA_GC Cuts at: 0 647 1593 Size: 647 946 BglII A'GATC_T Cuts at: 0 276 580 1593 Size: 276 304 1013 Fragments arranged by size: 1013 304 276 BlpI GC'TnA_GC Cuts at: 0 13 1593 Size: 13 1580 Bme1580I G_kGCm'C Cuts at: 0 203 792 1249 1593 Size: 203 589 457 344 Fragments arranged by size: 589 457 344 203 BmgBI CAC'GTC Cuts at: 0 9 229 1593 Size: 9 220 1364 Fragments arranged by size: 1364 220 9 BmrI ACTGGGnnnn_n' Cuts at: 0 1323 1593 Size: 1323 270 Bpu10I CC'TnA_GC Cuts at: 0 647 1593 Size: 647 946 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 350 1019 1292 1593 Size: 350 669 273 301 Fragments arranged by size: 669 350 301 273 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 56 86 984 1014 1593 Size: 56 30 898 30 579 Fragments arranged by size: 898 579 56 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 645 1593 Size: 645 948 BseYI C'CCAG_C Cuts at: 0 858 1010 1593 Size: 858 152 583 Fragments arranged by size: 858 583 152 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 251 1360 1593 Size: 251 1109 233 Fragments arranged by size: 1109 251 233 BsiHKAI G_wGCw'C Cuts at: 0 203 792 1593 Size: 203 589 801 Fragments arranged by size: 801 589 203 BsmBI CGTCTCn'nnnn_ Cuts at: 0 59 1593 Size: 59 1534 Bsp1286I G_dGCh'C Cuts at: 0 203 792 1249 1593 Size: 203 589 457 344 Fragments arranged by size: 589 457 344 203 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 813 1593 Size: 813 780 BsrGI T'GTAC_A Cuts at: 0 492 1593 Size: 492 1101 BstXI CCAn_nnnn'nTGG Cuts at: 0 567 1593 Size: 567 1026 BstYI r'GATC_y Cuts at: 0 276 580 828 1066 1455 1593 Size: 276 304 248 238 389 138 Fragments arranged by size: 389 304 276 248 238 138 BtgI C'CryG_G Cuts at: 0 945 1372 1593 Size: 945 427 221 Fragments arranged by size: 945 427 221 BtsI GCAGTG_nn' Cuts at: 0 1247 1593 Size: 1247 346 EaeI y'GGCC_r Cuts at: 0 1369 1593 Size: 1369 224 EarI CTCTTCn'nnn_ Cuts at: 0 422 1593 Size: 422 1171 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 19 1593 Size: 19 1574 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 337 542 1453 1593 Size: 337 205 911 140 Fragments arranged by size: 911 337 205 140 EcoNI CCTnn'n_nnAGG Cuts at: 0 1141 1593 Size: 1141 452 EcoO109I rG'GnC_Cy Cuts at: 0 1146 1345 1593 Size: 1146 199 248 Fragments arranged by size: 1146 248 199 EcoRV GAT'ATC Cuts at: 0 289 1593 Size: 289 1304 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 298 1593 Size: 298 1295 FspI TGC'GCA Cuts at: 0 841 1593 Size: 841 752 HaeII r_GCGC'y Cuts at: 0 335 1593 Size: 335 1258 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 572 604 656 688 964 984 996 1016 Size: 572 32 52 32 276 20 12 20 Cuts at: 1016 1593 Size: 577 Fragments arranged by size: 577 572 276 52 32 32 20 20 12 HincII GTy'rAC Cuts at: 0 308 1192 1593 Size: 308 884 401 Fragments arranged by size: 884 401 308 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 249 1593 Size: 249 1344 MscI TGG'CCA Cuts at: 0 1371 1593 Size: 1371 222 MspA1I CmG'CkG Cuts at: 0 862 1593 Size: 862 731 NcoI C'CATG_G Cuts at: 0 945 1372 1593 Size: 945 427 221 Fragments arranged by size: 945 427 221 PpuMI rG'GwC_Cy Cuts at: 0 1146 1345 1593 Size: 1146 199 248 Fragments arranged by size: 1146 248 199 PstI C_TGCA'G Cuts at: 0 384 1008 1098 1593 Size: 384 624 90 495 Fragments arranged by size: 624 495 384 90 PvuII CAG'CTG Cuts at: 0 862 1593 Size: 862 731 ScaI AGT'ACT Cuts at: 0 160 1593 Size: 160 1433 SfcI C'TryA_G Cuts at: 0 380 1004 1094 1141 1593 Size: 380 624 90 47 452 Fragments arranged by size: 624 452 380 90 47 SmlI C'TyrA_G Cuts at: 0 365 1034 1307 1584 1593 Size: 365 669 273 277 9 Fragments arranged by size: 669 365 277 273 9 SspI AAT'ATT Cuts at: 0 246 1593 Size: 246 1347 StyI C'CwwG_G Cuts at: 0 945 1372 1593 Size: 945 427 221 Fragments arranged by size: 945 427 221 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 1092 1593 Size: 1092 501 TatI w'GTAC_w Cuts at: 0 29 158 492 1593 Size: 29 129 334 1101 Fragments arranged by size: 1101 334 129 29 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 56 88 415 447 1593 Size: 56 32 327 32 1146 Fragments arranged by size: 1146 327 56 32 32 XbaI T'CTAG_A Cuts at: 0 600 1593 Size: 600 993 XmnI GAAnn'nnTTC Cuts at: 0 245 1593 Size: 245 1348 Enzymes that do cut: AccI AcuI AfeI AlfI AlwNI ApaLI ApoI AvaI BamHI BbsI BbvCI BglII BlpI Bme1580I BmgBI BmrI Bpu10I BpuEI BsaXI BseRI BseYI BsgI BsiHKAI BsmBI Bsp1286I BspMI BsrGI BstXI BstYI BtgI BtsI EaeI EarI EciI Eco57MI EcoNI EcoO109I EcoRV FalI FspI HaeII Hin4I HincII MmeI MscI MspA1I NcoI PpuMI PstI PvuII ScaI SfcI SmlI SspI StyI TaqII TatI TstI XbaI XmnI Enzymes that do not cut: AarI AatII Acc65I AclI AflII AflIII AgeI AhdI AleI AloI ApaI AscI AseI AsiSI AvrII BaeI BanI BanII BbeI BcgI BciVI BclI BfrBI BglI BmtI BplI BpmI BsaI BsaAI BsaBI BsaHI BsaWI BsiEI BsiWI BsmI BspEI BspHI BsrBI BsrDI BsrFI BssHII BssSI BstAPI BstBI BstEII BstZ17I Bsu36I BtgZI ClaI CspCI DraI DraIII DrdI EagI EcoICRI EcoRI FseI FspAI HindIII HpaI KasI KpnI MfeI MluI MslI NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI NspI PacI PasI PciI PflMI PfoI PmeI PmlI PpiI PshAI PsiI PspOMI PspXI PsrI PvuI RsrII SacI SacII SalI SanDI SapI SbfI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI StuI SwaI Tth111I XcmI XhoI XmaI ZraI