################################## # NO CUT ENZYMES ( 125 enzymes) # ################################## AatII Acc65I AccIII AclI AcyI AflII AgeI AhdI Alw44I AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp718I AspEI AspI AviII AvrII BalI BamHI BbeI BbrPI BbvCI BfrI BglI BglII BlnI BsaHI BsaWI BseAI BseRI BsiEI BsiWI BsmBI Bsp1407I BspDI BspEI BspHI BspLU11I BsrBI BsrGI BssHII BstAPI BstEII BstPI Bsu36I ClaI CpoI DraIII DrdI DsaI EaeI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoT22I FseI FspI HaeII HgaI Hin1I HincII HindII HindIII HpaI KasI KpnI KspI MfeI MluI MluNI MroI MscI MunI NarI NcoI NheI NotI NruI NsiI PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshBI Psp1406I PvuI RcaI RsrII SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SgrAI SmaI SnaBI SpeI Sse8387I SspBI StuI SwaI Tth111I Van91I XbaI XcmI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 37 enzymes) # ################################### AccI 1654 GTATAC GT^[AC][GT]AC AccII 24 CGCG CG^CG BanII 1258 GAGCTC G[GA]GC[CT]^C BcgI 1611 CGATAAGAATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BciVI 1038 GTATCC GTATCC(6/5) BclI 1911 TGATCA T^GATCA BfaI 1924 CTAG C^TAG BsaBI 804 GATAAGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaI 1603 GGTCTC GGTCTC(1/5) BsgI 683 CTGCAC GTGCAG(16/14) BspMI 245 GCAGGT ACCTGC(4/8) BsrDI 1773 CATTGC GCAATG(2/0) BsrFI 2009 GCCGGC [GA]^CCGG[CT] Bst1107I 1654 GTATAC GTA^TAC BstBI 467 TTCGAA TT^CGAA BstUI 24 CGCG CG^CG BstXI 1941 CCAGGATGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstZ17I 1654 GTATAC GTA^TAC Cfr10I 2009 GCCGGC [GA]^CCGG[CT] DraI 769 TTTAAA TTT^AAA EcoRV 1397 GATATC GAT^ATC EcoT14I 2033 CCAAGG C^C[AT][AT]GG FbaI 1911 TGATCA T^GATCA MaeI 1924 CTAG C^TAG MamI 804 GATAAGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MvnI 24 CGCG CG^CG NaeI 2009 GCCGGC GCC^GGC NgoMIV 2009 GCCGGC G^CCGGC NspV 467 TTCGAA TT^CGAA PleI 1917 GACTC GAGTC(4/5) PshAI 1597 GACTGGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PstI 433 CTGCAG CTGCA^G PvuII 1195 CAGCTG CAG^CTG SacI 1258 GAGCTC GAGCT^C SfuI 467 TTCGAA TT^CGAA SphI 997 GCATGC GCATG^C StyI 2033 CCAAGG C^C[AT][AT]GG ################################### # TWO CUT ENZYMES ( 29 enzymes) # ################################### AspHI 13 GAGCAC G[AT]GC[AT]^C 1258 GAGCTC AvaI 37 CCCGAG C^[CT]CG[GA]G 967 CTCGGG BanI 105 GGCACC G^G[CT][GA]CC 980 GGTGCC BbsI 2062 GAAGAC GAAGAC(2/6) 340 GTCTTC BcnI 1109 CCCGG CC^[GC]GG 1641 CCGGG BlpI 238 GCTCAGC GC^T[ACGT]AGC 676 GCTCAGC BmyI 13 GAGCAC G[AGT]GC[ACT]^C 1258 GAGCTC Bpu1102I 238 GCTCAGC GC^T[ACGT]AGC 676 GCTCAGC BpuAI 2062 GAAGAC GAAGAC(2/6) 340 GTCTTC BsaJI 966 CCTCGG C^C[ACGT][ACGT]GG 2033 CCAAGG BsiHKAI 13 GAGCAC G[AT]GC[AT]^C 1258 GAGCTC BsmI 1617 GAATGC GAATGC(1/-1) 1358 GCATTC BsoBI 37 CCCGAG C^[CT]CG[GA]G 967 CTCGGG Bsp1286I 13 GAGCAC G[AGT]GC[ACT]^C 1258 GAGCTC BssSI 509 CTCGTG CACGAG(-5/-1) 2039 CTCGTG BstYI 494 AGATCC [GA]^GATC[CT] 1584 GGATCT CelII 238 GCTCAGC GC^T[ACGT]AGC 676 GCTCAGC DraII 1280 AGGGCCT [GA]G^G[ACGT]CC[CT] 1414 AGGGCCT EcoO109I 1280 AGGGCCT [GA]G^G[ACGT]CC[CT] 1414 AGGGCCT EcoRI 838 GAATTC G^AATTC 1864 GAATTC FauI 1341 GCGGG CCCGC(4/6) 1878 GCGGG MflI 494 AGATCC [GA]^GATC[CT] 1584 GGATCT MspA1I 761 CCGCTG C[AC]G^C[GT]G 1195 CAGCTG NciI 1109 CCCGG CC^[GC]GG 1641 CCGGG NdeI 1031 CATATG CA^TATG 1331 CATATG NspI 559 ACATGC [GA]CATG^[CT] 997 GCATGC SfaNI 286 GATGC GCATC(5/9) 857 GCATC SmlI 1588 CTCAAG C^T[CT][GA]AG 1795 CTTGAG XhoII 494 AGATCC [GA]^GATC[CT] 1584 GGATCT ######################################### # 3 OR MORE CUT ENZYMES ( 67 enzymes) # ######################################### AciI AcsI AfaI AflIII AluI AlwI AlwNI ApoI Asp700I AvaII BbvI BpmI BsaAI BsiYI BslI BsmAI BsmFI BsrI BssKI BstF5I BstNI Cac8I CfoI Cfr13I DdeI DpnII EarI EcoRII Fnu4HI FokI HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeII MaeIII MboI MboII MnlI MseI MslI MspI MvaI MwoI NdeII NlaIII NlaIV RsaI Sau3AI Sau96I ScrFI SfcI SspI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XmnI