(Circular) (Six-base) MAPSORT of: pFN21AE3278.seq Check: 7395 from: 1 to: 6793 pFN21AE3278 6793 bp With 182 enzymes: SgfI * December 28, 2011 15:26 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4603 278 Size: 53 83 186 936 3067 2468 Fragments arranged by size: 3067 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3660 4136 1937 Size: 1723 476 4594 Fragments arranged by size: 4594 1723 476 Acc65I G'GTAC_C Cuts at: 4114 4114 Size: 6793 AclI AA'CG_TT Cuts at: 1563 4920 5293 1563 Size: 3357 373 3063 Fragments arranged by size: 3357 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2249 2261 2392 4856 6240 6508 1632 Size: 617 12 131 2464 1384 268 1917 Fragments arranged by size: 2464 1917 1384 617 268 131 12 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6596 AflIII A'CryG_T Cuts at: 1203 2368 2659 2868 5692 6669 1203 Size: 1165 291 209 2824 977 1327 Fragments arranged by size: 2824 1327 1165 977 291 209 AgeI A'CCGG_T Cuts at: 5624 5624 Size: 6793 AhdI GACnn_n'nnGTC Cuts at: 5522 5522 Size: 6793 AleI CACnn'nnGTG Cuts at: 1166 3038 3527 1166 Size: 1872 489 4432 Fragments arranged by size: 4432 1872 489 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 4025 1184 Size: 2841 3952 AlwNI CAG_nnn'CTG Cuts at: 2455 3394 3570 4536 6108 2455 Size: 939 176 966 1572 3140 Fragments arranged by size: 3140 1572 966 939 176 ApaLI G'TGCA_C Cuts at: 4850 6006 4850 Size: 1156 5637 ApoI r'AATT_y Cuts at: 1493 2458 2843 3318 3869 4102 4328 4480 6589 Size: 965 385 475 551 233 226 152 2109 Cuts at: 6589 1493 Size: 1697 Fragments arranged by size: 2109 1697 965 551 475 385 233 226 152 AseI AT'TA_AT Cuts at: 160 1032 5347 160 Size: 872 4315 1606 Fragments arranged by size: 4315 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6793 AvaI C'yCGr_G Cuts at: 1870 1943 2042 2972 4118 1870 Size: 73 99 930 1146 4545 Fragments arranged by size: 4545 1146 930 99 73 BamHI G'GATC_C Cuts at: 4123 4490 4123 Size: 367 6426 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2110 2985 4114 5569 618 Size: 325 217 627 323 875 1129 1455 1842 Fragments arranged by size: 1842 1455 1129 875 627 325 323 217 BanII G_rGCy'C Cuts at: 1391 3267 4112 1391 Size: 1876 845 4072 Fragments arranged by size: 4072 1876 845 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2338 4074 928 Size: 971 439 1736 3647 Fragments arranged by size: 3647 1736 971 439 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3605 3639 4984 5018 1903 Size: 34 1668 34 1345 34 3678 Fragments arranged by size: 3678 1668 1345 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3054 4685 5895 3054 Size: 1631 1210 3952 Fragments arranged by size: 3952 1631 1210 BclI T'GATC_A Cuts at: 1548 3916 1548 Size: 2368 4425 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5404 136 Size: 107 122 71 631 605 3732 1525 Fragments arranged by size: 3732 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6788 6788 Size: 6793 BlpI GC'TnA_GC Cuts at: 2244 2682 4210 2244 Size: 438 1528 4827 Fragments arranged by size: 4827 1528 438 Bme1580I G_kGCm'C Cuts at: 1790 4854 6010 1790 Size: 3064 1156 2573 Fragments arranged by size: 3064 2573 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6793 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2867 3613 4524 Size: 416 53 83 399 24 1450 746 911 Cuts at: 4524 5482 442 Size: 958 1753 Fragments arranged by size: 1753 1450 958 911 746 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6793 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2041 2041 Size: 6793 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2910 3648 3860 5453 1239 Size: 1671 738 212 1593 2579 Fragments arranged by size: 2579 1671 1593 738 212 Bpu10I CC'TnA_GC Cuts at: 2100 2990 2100 Size: 890 5903 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3578 3821 4918 5783 6081 3578 Size: 243 1097 865 298 4290 Fragments arranged by size: 4290 1097 865 298 243 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3614 5456 882 Size: 617 2115 1842 2219 Fragments arranged by size: 2219 2115 1842 617 BsaAI yAC'GTr Cuts at: 493 1204 2542 2660 2871 4584 493 Size: 711 1338 118 211 1713 2702 Fragments arranged by size: 2702 1713 1338 711 211 118 BsaBI GATnn'nnATC Cuts at: 2813 6787 2813 Size: 3974 2819 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4600 4982 5617 275 Size: 53 83 186 936 3067 382 635 1451 Fragments arranged by size: 3067 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5226 5624 5898 6045 6456 5226 Size: 398 274 147 411 5563 Fragments arranged by size: 5563 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6568 Fragments arranged by size: 6568 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 6793 BseYI C'CCAG_C Cuts at: 5996 6492 5996 Size: 496 6297 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2673 1163 Size: 573 937 5283 Fragments arranged by size: 5283 937 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4243 5004 6032 1540 Size: 275 186 2242 761 1028 2301 Fragments arranged by size: 2301 2242 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2022 3267 4854 4939 6010 2022 Size: 1245 1587 85 1071 2805 Fragments arranged by size: 2805 1587 1245 1071 85 BsmI GAATG_Cn' Cuts at: 3363 3628 4304 4397 3363 Size: 265 676 93 5759 Fragments arranged by size: 5759 676 265 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2022 3267 4112 4854 4939 6010 1391 Size: 399 232 1245 845 742 85 1071 2174 Fragments arranged by size: 2174 1245 1071 845 742 399 232 85 BspEI T'CCGG_A Cuts at: 6456 6456 Size: 6793 BspHI T'CATG_A Cuts at: 4682 6412 4682 Size: 1730 5063 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2241 4148 844 Size: 1397 1907 3489 Fragments arranged by size: 3489 1907 1397 BsrBI CCG'CTC Cuts at: 1394 1823 4680 1394 Size: 429 2857 3507 Fragments arranged by size: 3507 2857 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3777 5288 5462 65 Size: 1183 2529 1511 174 1396 Fragments arranged by size: 2529 1511 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4014 5437 5624 1700 Size: 110 2204 1423 187 2869 Fragments arranged by size: 2869 2204 1423 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6793 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6793 BssSI C'ACGA_G Cuts at: 2514 4044 4853 5865 2514 Size: 1530 809 1012 3442 Fragments arranged by size: 3442 1530 1012 809 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6793 BstBI TT'CG_AA Cuts at: 2473 5597 2473 Size: 3124 3669 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6793 BstXI CCAn_nnnn'nTGG Cuts at: 3953 3953 Size: 6793 BstYI r'GATC_y Cuts at: 1972 2499 3589 4123 4490 4875 4892 6344 6430 Size: 527 1090 534 367 385 17 1452 86 Cuts at: 6430 6442 6788 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 1090 534 527 385 367 346 86 17 12 BstZ17I GTA'TAC Cuts at: 3661 3661 Size: 6793 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 6241 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6054 Fragments arranged by size: 6054 683 56 BtsI GCAGTG_nn' Cuts at: 4309 5103 5123 4309 Size: 794 20 5979 Fragments arranged by size: 5979 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6793 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6758 DraI TTT'AAA Cuts at: 2776 4097 2776 Size: 1321 5472 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5800 809 Size: 507 4484 1802 Fragments arranged by size: 4484 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 4240 5129 6758 Size: 54 1425 50 275 18 2410 889 1629 Cuts at: 6758 8 Size: 43 Fragments arranged by size: 2410 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4240 1537 Size: 275 2428 4090 Fragments arranged by size: 4090 2428 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2163 3617 3645 4723 1353 Size: 810 1454 28 1078 3423 Fragments arranged by size: 3423 1454 1078 810 28 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3209 3824 5380 5754 5900 387 Size: 1064 138 1620 615 1556 374 146 1280 Fragments arranged by size: 1620 1556 1280 1064 615 374 146 138 EcoICRI GAG'CTC Cuts at: 3265 3265 Size: 6793 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2249 2261 2392 2910 3648 3860 4856 Size: 393 617 12 131 518 738 212 996 Cuts at: 4856 5453 6240 6508 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 996 787 738 617 597 518 393 268 212 131 12 EcoO109I rG'GnC_Cy Cuts at: 3286 3420 3286 Size: 134 6659 EcoRI G'AATT_C Cuts at: 2843 3869 4102 4480 2843 Size: 1026 233 378 5156 Fragments arranged by size: 5156 1026 378 233 EcoRV GAT'ATC Cuts at: 3404 3404 Size: 6793 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2362 3190 2362 Size: 828 5965 FspI TGC'GCA Cuts at: 5299 5299 Size: 6793 HaeII r_GCGC'y Cuts at: 1678 5940 1678 Size: 4262 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2264 2296 5434 5466 5508 Size: 32 135 32 692 32 3138 32 42 Cuts at: 5508 5540 1373 Size: 32 2626 Fragments arranged by size: 3138 2626 692 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4137 4383 669 Size: 988 281 2199 246 3079 Fragments arranged by size: 3079 2199 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6793 HpaI GTT'AAC Cuts at: 4383 4383 Size: 6793 KpnI G_GTAC'C Cuts at: 4118 4118 Size: 6793 MfeI C'AATT_G Cuts at: 4392 4392 Size: 6793 MluI A'CGCG_T Cuts at: 6669 6669 Size: 6793 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2398 2858 5907 6091 1305 Size: 1093 460 3049 184 2007 Fragments arranged by size: 3049 2007 1093 460 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4971 Fragments arranged by size: 4971 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2695 3038 3527 3893 4751 5110 5269 Size: 648 1529 343 489 366 858 359 159 Cuts at: 5269 518 Size: 2042 Fragments arranged by size: 2042 1529 858 648 489 366 359 343 159 MspA1I CmG'CkG Cuts at: 1606 2768 3202 4209 4886 6034 6279 1606 Size: 1162 434 1007 677 1148 245 2120 Fragments arranged by size: 2120 1162 1148 1007 677 434 245 NaeI GCC'GGC Cuts at: 4016 4016 Size: 6793 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 6241 NdeI CA'TA_TG Cuts at: 387 3037 3337 387 Size: 2650 300 3843 Fragments arranged by size: 3843 2650 300 NgoMIV G'CCGG_C Cuts at: 4014 4014 Size: 6793 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6793 NotI GC'GGCC_GC Cuts at: 4240 4240 Size: 6793 NspI r_CATG'y Cuts at: 1132 1229 2568 3006 4151 5696 1132 Size: 97 1339 438 1145 1545 2229 Fragments arranged by size: 2229 1545 1339 1145 438 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6793 PciI A'CATG_T Cuts at: 5692 5692 Size: 6793 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6793 PfoI T'CCnGG_A Cuts at: 2502 3645 2502 Size: 1143 5650 PmeI GTTT'AAAC Cuts at: 4097 4097 Size: 6793 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4862 4894 6375 6407 4862 Size: 32 1481 32 5248 Fragments arranged by size: 5248 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 3606 1613 Size: 1993 4800 PsiI TTA'TAA Cuts at: 4363 4363 Size: 6793 PstI C_TGCA'G Cuts at: 830 2442 4145 830 Size: 1612 1703 3478 Fragments arranged by size: 3478 1703 1612 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6793 PvuII CAG'CTG Cuts at: 3202 3202 Size: 6793 SacI G_AGCT'C Cuts at: 3267 3267 Size: 6793 SalI G'TCGA_C Cuts at: 1936 4135 1936 Size: 2199 4594 SbfI CC_TGCA'GG Cuts at: 4145 4145 Size: 6793 ScaI AGT'ACT Cuts at: 5041 6753 5041 Size: 1712 5081 SfcI C'TryA_G Cuts at: 826 1045 2438 2604 3253 4141 5276 5957 6148 Size: 219 1393 166 649 888 1135 681 191 Cuts at: 6148 826 Size: 1471 Fragments arranged by size: 1471 1393 1135 888 681 649 219 191 166 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6793 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6793 SmaI CCC'GGG Cuts at: 1872 4120 1872 Size: 2248 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3593 3800 4897 5798 6060 820 Size: 197 926 1650 207 1097 901 262 1553 Fragments arranged by size: 1650 1553 1097 926 901 262 207 197 SnaBI TAC'GTA Cuts at: 493 4584 493 Size: 4091 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6793 SphI G_CATG'C Cuts at: 1132 3006 4151 1132 Size: 1874 1145 3774 Fragments arranged by size: 3774 1874 1145 SspI AAT'ATT Cuts at: 5 52 2386 2733 2749 4717 5 Size: 47 2334 347 16 1968 2081 Fragments arranged by size: 2334 2081 1968 347 47 16 StuI AGG'CCT Cuts at: 1517 1517 Size: 6793 StyI C'CwwG_G Cuts at: 513 1065 4038 4232 513 Size: 552 2973 194 3074 Fragments arranged by size: 3074 2973 552 194 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3969 4820 4837 4990 5175 5620 325 Size: 1255 2389 851 17 153 185 445 1498 Fragments arranged by size: 2389 1498 1255 851 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2740 2902 3990 Size: 274 80 33 51 1443 763 162 1088 Cuts at: 3990 5039 6751 96 Size: 1049 1712 138 Fragments arranged by size: 1712 1443 1088 1049 763 274 162 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6753 6785 6753 Size: 32 6761 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6793 XbaI T'CTAG_A Cuts at: 4129 4129 Size: 6793 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6793 XmaI C'CCGG_G Cuts at: 1870 4118 1870 Size: 2248 4545 XmnI GAAnn'nnTTC Cuts at: 2177 3638 3830 4922 2177 Size: 1461 192 1092 4048 Fragments arranged by size: 4048 1461 1092 192 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4601 276 Size: 53 83 186 936 3067 2468 Fragments arranged by size: 3067 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BsiWI BsmBI Bsu36I DraIII EcoNI FseI FspAI KasI NarI NruI NsiI PacI PmlI PpuMI PspOMI PspXI PsrI RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI