Restriction map for
pFN21AE3278
(Linear) (Six-base) MAPSORT of: KIEE3278.seq Check: 7437 from: 1 to: 2088
KIEE3278.orf
With 182 enzymes: SgfI *
December 27, 2011 16:34 ..
AccI GT'mk_AC
Cuts at: 0 1655 2088
Size: 1655 433
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 244 256 387 2088
Size: 244 12 131 1701
Fragments arranged by size:
1701 244 131 12
AflIII A'CryG_T
Cuts at: 0 363 654 863 2088
Size: 363 291 209 1225
Fragments arranged by size:
1225 363 291 209
AleI CACnn'nnGTG
Cuts at: 0 1033 1522 2088
Size: 1033 489 566
Fragments arranged by size:
1033 566 489
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2020 2088
Size: 2020 68
AlwNI CAG_nnn'CTG
Cuts at: 0 450 1389 1565 2088
Size: 450 939 176 523
Fragments arranged by size:
939 523 450 176
ApoI r'AATT_y
Cuts at: 0 453 838 1313 1864 2088
Size: 453 385 475 551 224
Fragments arranged by size:
551 475 453 385 224
AvaI C'yCGr_G
Cuts at: 0 37 967 2088
Size: 37 930 1121
Fragments arranged by size:
1121 930 37
BanI G'GyrC_C
Cuts at: 0 105 980 2088
Size: 105 875 1108
Fragments arranged by size:
1108 875 105
BanII G_rGCy'C
Cuts at: 0 1262 2088
Size: 1262 826
BbsI GAAGACnn'nnnn_
Cuts at: 0 333 2069 2088
Size: 333 1736 19
Fragments arranged by size:
1736 333 19
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1600 1634 2088
Size: 1600 34 454
Fragments arranged by size:
1600 454 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 1049 2088
Size: 1049 1039
BclI T'GATC_A
Cuts at: 0 1911 2088
Size: 1911 177
BlpI GC'TnA_GC
Cuts at: 0 239 677 2088
Size: 239 438 1411
Fragments arranged by size:
1411 438 239
BmrI ACTGGGnnnn_n'
Cuts at: 0 862 1608 2088
Size: 862 746 480
Fragments arranged by size:
862 746 480
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 36 2088
Size: 36 2052
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 905 1643 1855 2088
Size: 905 738 212 233
Fragments arranged by size:
905 738 233 212
Bpu10I CC'TnA_GC
Cuts at: 0 95 985 2088
Size: 95 890 1103
Fragments arranged by size:
1103 890 95
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1573 1816 2088
Size: 1573 243 272
Fragments arranged by size:
1573 272 243
BsaI GGTCTCn'nnnn_
Cuts at: 0 1609 2088
Size: 1609 479
BsaAI yAC'GTr
Cuts at: 0 537 655 866 2088
Size: 537 118 211 1222
Fragments arranged by size:
1222 537 211 118
BsaBI GATnn'nnATC
Cuts at: 0 808 2088
Size: 808 1280
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 668 2088
Size: 668 1420
BsiHKAI G_wGCw'C
Cuts at: 0 17 1262 2088
Size: 17 1245 826
Fragments arranged by size:
1245 826 17
BsmI GAATG_Cn'
Cuts at: 0 1358 1623 2088
Size: 1358 265 465
Fragments arranged by size:
1358 465 265
Bsp1286I G_dGCh'C
Cuts at: 0 17 1262 2088
Size: 17 1245 826
Fragments arranged by size:
1245 826 17
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 236 2088
Size: 236 1852
BsrDI GCAATG_nn'
Cuts at: 0 1772 2088
Size: 1772 316
BsrFI r'CCGG_y
Cuts at: 0 2009 2088
Size: 2009 79
BssSI C'ACGA_G
Cuts at: 0 509 2039 2088
Size: 509 1530 49
Fragments arranged by size:
1530 509 49
BstBI TT'CG_AA
Cuts at: 0 468 2088
Size: 468 1620
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1948 2088
Size: 1948 140
BstYI r'GATC_y
Cuts at: 0 494 1584 2088
Size: 494 1090 504
Fragments arranged by size:
1090 504 494
BstZ17I GTA'TAC
Cuts at: 0 1656 2088
Size: 1656 432
DraI TTT'AAA
Cuts at: 0 771 2088
Size: 771 1317
EarI CTCTTCn'nnn_
Cuts at: 0 158 1612 1640 2088
Size: 158 1454 28 448
Fragments arranged by size:
1454 448 158 28
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1204 1819 2088
Size: 1204 615 269
Fragments arranged by size:
1204 615 269
EcoICRI GAG'CTC
Cuts at: 0 1260 2088
Size: 1260 828
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 244 256 387 905 1643 1855 2088
Size: 244 12 131 518 738 212 233
Fragments arranged by size:
738 518 244 233 212 131 12
EcoO109I rG'GnC_Cy
Cuts at: 0 1281 1415 2088
Size: 1281 134 673
Fragments arranged by size:
1281 673 134
EcoRI G'AATT_C
Cuts at: 0 838 1864 2088
Size: 838 1026 224
Fragments arranged by size:
1026 838 224
EcoRV GAT'ATC
Cuts at: 0 1399 2088
Size: 1399 689
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 357 1185 2088
Size: 357 828 903
Fragments arranged by size:
903 828 357
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 259 291 2088
Size: 259 32 1797
Fragments arranged by size:
1797 259 32
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 393 853 2088
Size: 393 460 1235
Fragments arranged by size:
1235 460 393
MslI CAynn'nnrTG
Cuts at: 0 690 1033 1522 1888 2088
Size: 690 343 489 366 200
Fragments arranged by size:
690 489 366 343 200
MspA1I CmG'CkG
Cuts at: 0 763 1197 2088
Size: 763 434 891
Fragments arranged by size:
891 763 434
NaeI GCC'GGC
Cuts at: 0 2011 2088
Size: 2011 77
NdeI CA'TA_TG
Cuts at: 0 1032 1332 2088
Size: 1032 300 756
Fragments arranged by size:
1032 756 300
NgoMIV G'CCGG_C
Cuts at: 0 2009 2088
Size: 2009 79
NspI r_CATG'y
Cuts at: 0 563 1001 2088
Size: 563 438 1087
Fragments arranged by size:
1087 563 438
PfoI T'CCnGG_A
Cuts at: 0 497 1640 2088
Size: 497 1143 448
Fragments arranged by size:
1143 497 448
PshAI GACnn'nnGTC
Cuts at: 0 1601 2088
Size: 1601 487
PstI C_TGCA'G
Cuts at: 0 437 2088
Size: 437 1651
PvuII CAG'CTG
Cuts at: 0 1197 2088
Size: 1197 891
SacI G_AGCT'C
Cuts at: 0 1262 2088
Size: 1262 826
SfcI C'TryA_G
Cuts at: 0 433 599 1248 2088
Size: 433 166 649 840
Fragments arranged by size:
840 649 433 166
SmlI C'TyrA_G
Cuts at: 0 1588 1795 2088
Size: 1588 207 293
Fragments arranged by size:
1588 293 207
SphI G_CATG'C
Cuts at: 0 1001 2088
Size: 1001 1087
SspI AAT'ATT
Cuts at: 0 381 728 744 2088
Size: 381 347 16 1344
Fragments arranged by size:
1344 381 347 16
StyI C'CwwG_G
Cuts at: 0 2033 2088
Size: 2033 55
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1964 2088
Size: 1964 124
TatI w'GTAC_w
Cuts at: 0 735 897 1985 2088
Size: 735 162 1088 103
Fragments arranged by size:
1088 735 162 103
XmnI GAAnn'nnTTC
Cuts at: 0 172 1633 1825 2088
Size: 172 1461 192 263
Fragments arranged by size:
1461 263 192 172
Enzymes that do cut:
AccI AcuI AflIII AleI AlfI AlwNI ApoI AvaI
BanI BanII BbsI BcgI BciVI BclI BlpI BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsgI
BsiHKAI BsmI Bsp1286I BspMI BsrDI BsrFI BssSI BstBI
BstXI BstYI BstZ17I DraI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI EcoRV FalI Hin4I MmeI MslI MspA1I
NaeI NdeI NgoMIV NspI PfoI PshAI PstI PvuII
SacI SfcI SmlI SphI SspI StyI TaqII TatI
XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AgeI AhdI
AloI ApaI ApaLI AscI AseI AsiSI AvrII BaeI
BamHI BbeI BbvCI BfrBI BglI BglII Bme1580I BmgBI
BmtI BsaHI BsaWI BsaXI BseRI BseYI BsiEI BsiWI
BsmBI BspEI BspHI BsrBI BsrGI BssHII BstAPI BstEII
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraIII DrdI
EaeI EagI EcoNI FseI FspI FspAI HaeII HincII
HindIII HpaI KasI KpnI MfeI MluI MscI NarI
NcoI NheI NotI NruI NsiI PacI PasI PciI
PflMI PmeI PmlI PpiI PpuMI PsiI PspOMI PspXI
PsrI PvuI RsrII SacII SalI SanDI SapI SbfI
ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI
SpeI SrfI StuI SwaI TstI Tth111I XbaI XcmI
XhoI XmaI ZraI