################################## # NO CUT ENZYMES ( 80 enzymes) # ################################## AatII AclI AflII AgeI AhdI Aor51HI AscI AseI AsnI Asp700I AspEI AviII AvrII BamHI BbeI BciVI BfrI BlnI BsaBI BsiEI BsiWI BspLU11I BsrBI BsrFI BssHII Bst1107I BstBI BstZ17I Cfr10I CpoI DraI DrdI EagI Eam1105I EclXI Eco47III Eco52I EcoRV EcoT22I FseI FspI HaeII HindIII HpaI KasI KspI MamI MfeI MluI MunI NaeI NarI NdeI NgoMIV NheI NotI NsiI NspV PacI PinAI PmeI PshAI PshBI Psp1406I PvuI RsrII SacI SacII SalI ScaI SexAI SfiI SfoI SfuI SgrAI SnaBI SpeI SspI SwaI XmnI ################################### # ONE CUT ENZYMES ( 40 enzymes) # ################################### AccI 1183 GTCTAC GT^[AC][GT]AC AccIII 3572 TCCGGA T^CCGGA AcyI 3818 GACGCC G[GA]^CG[CT]C AflIII 200 ACGTGT A^C[GA][CT]GT Alw44I 2845 GTGCAC G^TGCAC AocI 239 CCTCAGG CC^T[ACGT]AGG ApaI 957 GGGCCC GGGCC^C ApaLI 2845 GTGCAC G^TGCAC AspI 2998 GACGGAGTC GAC[ACGT]^[ACGT][ACGT]GTC BcgI 454 CGAGACCAGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BclI 74 TGATCA T^GATCA BsaHI 3818 GACGCC G[GA]^CG[CT]C BseAI 3572 TCCGGA T^CCGGA BspDI 1780 ATCGAT AT^CGAT BspEI 3572 TCCGGA T^CCGGA BspMI 3029 ACCTGC ACCTGC(4/8) BssSI 322 CACGAG CACGAG(-5/-1) Bsu36I 239 CCTCAGG CC^T[ACGT]AGG ClaI 1780 ATCGAT AT^CGAT DraIII 3342 CACCACGTG CAC[ACGT][ACGT][ACGT]^GTG Eco81I 239 CCTCAGG CC^T[ACGT]AGG EcoNI 3719 CCTACGACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoRI 2749 GAATTC G^AATTC FbaI 74 TGATCA T^GATCA Hin1I 3818 GACGCC G[GA]^CG[CT]C MroI 3572 TCCGGA T^CCGGA NruI 1536 TCGCGA TCG^CGA PflFI 2998 GACGGAGTC GAC[ACGT]^[ACGT][ACGT]GTC PflMI 2164 CCATCCTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PvuII 94 CAGCTG CAG^CTG SapI 757 GAAGAGC GCTCTTC(1/4) SbfI 3324 CCTGCAGG CCTGCA^GG SmaI 722 CCCGGG CCC^GGG SphI 2481 GCATGC GCATG^C Sse8387I 3324 CCTGCAGG CCTGCA^GG Tth111I 2998 GACGGAGTC GAC[ACGT]^[ACGT][ACGT]GTC Van91I 2164 CCATCCTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XbaI 2229 TCTAGA T^CTAGA XmaCI 722 CCCGGG C^CCGGG XmaI 722 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 30 enzymes) # ################################### AccII 1537 CGCG CG^CG 3679 CGCG AcsI 2749 GAATTC [GA]^AATT[CT] 3013 GAATTT ApoI 2749 GAATTC [GA]^AATT[CT] 3013 GAATTT AspHI 2845 GTGCAC G[AT]GC[AT]^C 3319 GTGCTC BbrPI 199 CACGTG CAC^GTG 3345 CACGTG BbvCI 1206 CCTCAGC CCTCAGC(-5/-2) 3801 CCTCAGC BglI 1225 GCCAAGGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 1616 GCCTGGTGGGC BglII 1962 AGATCT A^GATCT 2226 AGATCT BsaAI 199 CACGTG [CT]AC^GT[GA] 3345 CACGTG BsiHKAI 2845 GTGCAC G[AT]GC[AT]^C 3319 GTGCTC BsmBI 1497 CGTCTC CGTCTC(1/5) 4036 CGTCTC BsmI 894 GAATGC GAATGC(1/-1) 2040 GAATGC BspHI 1908 TCATGA T^CATGA 2006 TCATGA BsrDI 150 CATTGC GCAATG(2/0) 2618 GCAATG BstEII 102 GGTCACC G^GT[ACGT]ACC 333 GGTCACC BstPI 102 GGTCACC G^GT[ACGT]ACC 333 GGTCACC BstUI 1537 CGCG CG^CG 3679 CGCG BstXI 1022 CCATTGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 2699 CCACCCAAGTGG EcoO65I 102 GGTCACC G^GT[ACGT]ACC 333 GGTCACC HincII 2995 GTTGAC GT[CT]^[GA]AC 3025 GTTGAC HindII 2995 GTTGAC GT[CT]^[GA]AC 3025 GTTGAC MvnI 1537 CGCG CG^CG 3679 CGCG NcoI 2541 CCATGG C^CATGG 2855 CCATGG PaeR7I 1580 CTCGAG C^TCGAG 1834 CTCGAG PmaCI 199 CACGTG CAC^GTG 3345 CACGTG PmlI 199 CACGTG CAC^GTG 3345 CACGTG PpuMI 1382 AGGTCCC [GA]G^G[AT]CC[CT] 2206 AGGACCT RcaI 1908 TCATGA T^CATGA 2006 TCATGA StuI 251 AGGCCT AGG^CCT 368 AGGCCT XhoI 1580 CTCGAG C^TCGAG 1834 CTCGAG ######################################### # 3 OR MORE CUT ENZYMES ( 108 enzymes) # ######################################### Acc65I AciI AfaI AluI AlwI AlwNI Asp718I AvaI AvaII BalI BanI BanII BbsI BbvI BcnI BfaI BlpI BmyI BpmI Bpu1102I BpuAI BsaI BsaJI BsaWI BseRI BsgI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I Bsp1407I BsrGI BsrI BssKI BstAPI BstF5I BstNI BstYI Cac8I CelII CfoI Cfr13I DdeI DpnII DraII DsaI EaeI EarI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HgaI HhaI HinP1I HinfI HpaII HphI ItaI KpnI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV NspI PleI PstI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI SspBI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XcmI XhoII