Restriction map for
pFN21AE2668
(Circular) (Six-base) MAPSORT of: pFN21AE2668.seq Check: 6914 from: 1 to: 8779
pFN21AE2668 8779 bp
With 182 enzymes: SgfI *
January 26, 2017 18:09 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 6589 278
Size: 53 83 186 936 5053 2468
Fragments arranged by size:
5053 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3189 6122 1937
Size: 1252 2933 4594
Fragments arranged by size:
4594 2933 1252
Acc65I G'GTAC_C
Cuts at: 2840 3973 5973 6100 2840
Size: 1133 2000 127 5519
Fragments arranged by size:
5519 2000 1133 127
AclI AA'CG_TT
Cuts at: 1563 6906 7279 1563
Size: 5343 373 3063
Fragments arranged by size:
5343 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2054 2073 6842 8226 8494 1632
Size: 422 19 4769 1384 268 1917
Fragments arranged by size:
4769 1917 1384 422 268 19
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 8582
AflIII A'CryG_T
Cuts at: 1203 2205 7678 8655 1203
Size: 1002 5473 977 1327
Fragments arranged by size:
5473 1327 1002 977
AgeI A'CCGG_T
Cuts at: 7610 7610
Size: 8779
AhdI GACnn_n'nnGTC
Cuts at: 7508 7508
Size: 8779
AleI CACnn'nnGTG
Cuts at: 1166 2529 4709 1166
Size: 1363 2180 5236
Fragments arranged by size:
5236 2180 1363
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 8779
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5609 5641 5609
Size: 32 8747
AlwNI CAG_nnn'CTG
Cuts at: 2404 2413 2896 3535 3694 4982 6522 8094 2404
Size: 9 483 639 159 1288 1540 1572 3089
Fragments arranged by size:
3089 1572 1540 1288 639 483 159 9
ApaI G_GGCC'C
Cuts at: 2966 2966
Size: 8779
ApaLI G'TGCA_C
Cuts at: 4850 6836 7992 4850
Size: 1986 1156 5637
Fragments arranged by size:
5637 1986 1156
ApoI r'AATT_y
Cuts at: 1493 4754 5018 6088 6314 6466 8575 1493
Size: 3261 264 1070 226 152 2109 1697
Fragments arranged by size:
3261 2109 1697 1070 264 226 152
AseI AT'TA_AT
Cuts at: 160 1032 7333 160
Size: 872 6301 1606
Fragments arranged by size:
6301 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8779
AvaI C'yCGr_G
Cuts at: 1870 1943 2727 3585 3839 4071 6104 1870
Size: 73 784 858 254 232 2033 4545
Fragments arranged by size:
4545 2033 858 784 254 232 73
BamHI G'GATC_C
Cuts at: 6109 6476 6109
Size: 367 8412
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2706 2799 2840 3121 3973
Size: 325 217 627 919 93 41 281 852
Cuts at: 3973 4735 4927 5096 5973 6100 7555 618
Size: 762 192 169 877 127 1455 1842
Fragments arranged by size:
1842 1455 919 877 852 762 627 325
281 217 192 169 127 93 41
BanII G_rGCy'C
Cuts at: 1391 2075 2610 2736 2769 2937 2966 3315 4569
Size: 684 535 126 33 168 29 349 1254
Cuts at: 4569 4580 4661 6098 1391
Size: 11 81 1437 4072
Fragments arranged by size:
4072 1437 1254 684 535 349 168 126
81 33 29 11
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2188 2292 5280 928
Size: 971 289 104 2988 4427
Fragments arranged by size:
4427 2988 971 289 104
BbvCI CC'TCA_GC
Cuts at: 3212 5807 3212
Size: 2595 6184
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2448 2482 6970 7004 1903
Size: 34 511 34 4488 34 3678
Fragments arranged by size:
4488 3678 511 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 6671 7881 6671
Size: 1210 7569
BclI T'GATC_A
Cuts at: 1548 2079 1548
Size: 531 8248
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 3236 3627
Size: 107 122 71 631 605 337 1227 391
Cuts at: 3627 7390 136
Size: 3763 1525
Fragments arranged by size:
3763 1525 1227 631 605 391 337 122
107 71
BglII A'GATC_T
Cuts at: 3967 4231 8774 3967
Size: 264 4543 3972
Fragments arranged by size:
4543 3972 264
BlpI GC'TnA_GC
Cuts at: 2265 2417 2974 5483 6196 2265
Size: 152 557 2509 713 4848
Fragments arranged by size:
4848 2509 713 557 152
Bme1580I G_kGCm'C
Cuts at: 1790 2226 2711 2804 2966 4740 4854 5176 5422
Size: 436 485 93 162 1774 114 322 246
Cuts at: 5422 6840 7996 1790
Size: 1418 1156 2573
Fragments arranged by size:
2573 1774 1418 1156 485 436 322 246
162 114 93
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 8779
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 6510 7468 442
Size: 416 53 83 399 24 5093 958 1753
Fragments arranged by size:
5093 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 8779
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3334 3334
Size: 8779
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2123 2456 2614 2973 3089 3095 3147 3179
Size: 884 333 158 359 116 6 52 32
Cuts at: 3179 4351 4675 5004 5312 7439 1239
Size: 1172 324 329 308 2127 2579
Fragments arranged by size:
2579 2127 1172 884 359 333 329 324
308 158 116 52 32 6
Bpu10I CC'TnA_GC
Cuts at: 3212 3384 3671 5807 3212
Size: 172 287 2136 6184
Fragments arranged by size:
6184 2136 287 172
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3243 6904 7769 8067 3243
Size: 3661 865 298 3955
Fragments arranged by size:
3955 3661 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2454 3173 3281 4068 4094 4468 4654
Size: 617 955 719 108 787 26 374 186
Cuts at: 4654 4982 7442 882
Size: 328 2460 2219
Fragments arranged by size:
2460 2219 955 787 719 617 374 328
186 108 26
BsaAI yAC'GTr
Cuts at: 493 1204 2206 5352 6570 493
Size: 711 1002 3146 1218 2702
Fragments arranged by size:
3146 2702 1218 1002 711
BsaBI GATnn'nnATC
Cuts at: 8773 8773
Size: 8779
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 5824 6586 6968 7603
Size: 53 83 186 936 4291 762 382 635
Cuts at: 7603 275
Size: 1451
Fragments arranged by size:
4291 1451 936 762 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2703 5577 5916 7212 7610 7884 8031 8442 2703
Size: 2874 339 1296 398 274 147 411 3040
Fragments arranged by size:
3040 2874 1296 411 398 339 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2225 2255 2708 2738 3290
Size: 30 165 30 822 30 453 30 552
Cuts at: 3290 3320 5285 5315 5379 5409 5609 5639 1178
Size: 30 1965 30 64 30 200 30 4318
Fragments arranged by size:
4318 1965 822 552 453 200 165 64
30 30 30 30 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2156 2159 2233 2870 2942 3246 4084 4354
Size: 970 3 74 637 72 304 838 270
Cuts at: 4354 4457 1186
Size: 103 5508
Fragments arranged by size:
5508 970 838 637 304 270 103 74
72 3
BseYI C'CCAG_C
Cuts at: 2620 3392 4402 4777 5421 5632 7982 8478 2620
Size: 772 1010 375 644 211 2350 496 2921
Fragments arranged by size:
2921 2350 1010 772 644 496 375 211
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2301 3323 3707 4328 1163
Size: 573 565 1022 384 621 5614
Fragments arranged by size:
5614 1022 621 573 565 384
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 6229 6990 8018 1540
Size: 275 186 4228 761 1028 2301
Fragments arranged by size:
4228 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4854 5328 6840 6925 7996 4854
Size: 474 1512 85 1071 5637
Fragments arranged by size:
5637 1512 1071 474 85
BsmI GAATG_Cn'
Cuts at: 2905 4051 6290 6383 2905
Size: 1146 2239 93 5301
Fragments arranged by size:
5301 2239 1146 93
BsmBI CGTCTCn'nnnn_
Cuts at: 3508 6047 3508
Size: 2539 6240
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2075 2226 2610 2711 2736 2769 2804
Size: 399 285 151 384 101 25 33 35
Cuts at: 2804 2937 2966 3315 4569 4580 4661 4740 4854
Size: 133 29 349 1254 11 81 79 114
Cuts at: 4854 5176 5328 5422 6098 6840 6925 7996 1391
Size: 322 152 94 676 742 85 1071 2174
Fragments arranged by size:
2174 1254 1071 742 676 399 384 349
322 285 152 151 133 114 101 94
85 81 79 35 33 29 25 11
BspEI T'CCGG_A
Cuts at: 5577 8442 5577
Size: 2865 5914
BspHI T'CATG_A
Cuts at: 3913 4011 6668 8398 3913
Size: 98 2657 1730 4294
Fragments arranged by size:
4294 2657 1730 98
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 5043 6134 844
Size: 4199 1091 3489
Fragments arranged by size:
4199 3489 1091
BsrBI CCG'CTC
Cuts at: 1394 1823 6666 1394
Size: 429 4843 3507
Fragments arranged by size:
4843 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2154 4630 7274 7448 65
Size: 1183 906 2476 2644 174 1396
Fragments arranged by size:
2644 2476 1396 1183 906 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 7423 7610 1700
Size: 110 5613 187 2869
Fragments arranged by size:
5613 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2208 3885 5760 96
Size: 2112 1677 1875 3115
Fragments arranged by size:
3115 2112 1875 1677
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 8779
BssSI C'ACGA_G
Cuts at: 2327 6839 7851 2327
Size: 4512 1012 3255
Fragments arranged by size:
4512 3255 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2413 2598 3121 3694 4030 794
Size: 1619 185 523 573 336 5543
Fragments arranged by size:
5543 1619 573 523 336 185
BstBI TT'CG_AA
Cuts at: 7583 7583
Size: 8779
BstEII G'GTnAC_C
Cuts at: 1184 2107 2338 1184
Size: 923 231 7625
Fragments arranged by size:
7625 923 231
BstXI CCAn_nnnn'nTGG
Cuts at: 3034 4711 3034
Size: 1677 7102
BstYI r'GATC_y
Cuts at: 1972 3531 3967 4231 4876 6109 6476 6861 6878
Size: 1559 436 264 645 1233 367 385 17
Cuts at: 6878 8330 8416 8428 8774 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1559 1452 1233 645 436 385 367
346 264 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2245 2245
Size: 8779
BtgI C'CryG_G
Cuts at: 513 1065 2004 2536 3294 4546 4860 513
Size: 552 939 532 758 1252 314 4432
Fragments arranged by size:
4432 1252 939 758 552 532 314
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3411 3558 487
Size: 683 56 2185 147 5708
Fragments arranged by size:
5708 2185 683 147 56
BtsI GCAGTG_nn'
Cuts at: 2524 2536 2897 2914 3063 3225 3311 4266 4925
Size: 12 361 17 149 162 86 955 659
Cuts at: 4925 5790 5868 6295 7089 7109 2524
Size: 865 78 427 794 20 4194
Fragments arranged by size:
4194 955 865 794 659 427 361 162
149 86 78 20 17 12
ClaI AT'CG_AT
Cuts at: 1554 3786 1554
Size: 2232 6547
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3496 3531 4114 4149 4576 4611 529
Size: 35 2932 35 583 35 427 35 4697
Fragments arranged by size:
4697 2932 583 427 35 35 35 35
DraI TTT'AAA
Cuts at: 6083 6083
Size: 8779
DraIII CAC_nnn'GTG
Cuts at: 5352 5352
Size: 8779
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 7786 809
Size: 507 6470 1802
Fragments arranged by size:
6470 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2533 2653 2881
Size: 54 1425 50 275 18 703 120 228
Cuts at: 2881 3237 3495 5560 5701 6226 7115 8744 8
Size: 356 258 2065 141 525 889 1629 43
Fragments arranged by size:
2065 1629 1425 889 703 525 356 275
258 228 141 120 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 6226 1537
Size: 275 4414 4090
Fragments arranged by size:
4414 4090 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2757 2943 4443 6709 1353
Size: 1404 186 1500 2266 3423
Fragments arranged by size:
3423 2266 1500 1404 186
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 7366 7740 7886 387
Size: 1064 138 5777 374 146 1280
Fragments arranged by size:
5777 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2054 2073 2123 2456 2614 2973 3089
Size: 393 422 19 50 333 158 359 116
Cuts at: 3089 3095 3147 3179 4351 4675 5004 5312 6842
Size: 6 52 32 1172 324 329 308 1530
Cuts at: 6842 7439 8226 8494 1239
Size: 597 787 268 1524
Fragments arranged by size:
1530 1524 1172 787 597 422 393 359
333 329 324 308 268 158 116 52
50 32 19 6
EcoNI CCTnn'n_nnAGG
Cuts at: 5728 5728
Size: 8779
EcoO109I rG'GnC_Cy
Cuts at: 2962 2963 3100 3388 3691 4212 5296 2962
Size: 1 137 288 303 521 1084 6445
Fragments arranged by size:
6445 1084 521 303 288 137 1
EcoRI G'AATT_C
Cuts at: 4754 6088 6466 4754
Size: 1334 378 7067
Fragments arranged by size:
7067 1334 378
FspI TGC'GCA
Cuts at: 7285 7285
Size: 8779
HaeII r_GCGC'y
Cuts at: 1678 7926 1678
Size: 6248 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2708 2740 3389 3421 3568
Size: 32 135 32 1136 32 649 32 147
Cuts at: 3568 3579 3600 3611 4199 4231 4977 5009 5720
Size: 11 21 11 588 32 746 32 711
Cuts at: 5720 5752 7420 7452 7494 7526 1373
Size: 32 1668 32 42 32 2626
Fragments arranged by size:
2626 1668 1136 746 711 649 588 147
135 42 32 32 32 32 32 32
32 32 32 21 11 11
HincII GTy'rAC
Cuts at: 669 1657 1938 5002 5032 6123 6369 669
Size: 988 281 3064 30 1091 246 3079
Fragments arranged by size:
3079 3064 1091 988 281 246 30
HindIII A'AGCT_T
Cuts at: 748 748
Size: 8779
HpaI GTT'AAC
Cuts at: 6369 6369
Size: 8779
KpnI G_GTAC'C
Cuts at: 2844 3977 5977 6104 2844
Size: 1133 2000 127 5519
Fragments arranged by size:
5519 2000 1133 127
MfeI C'AATT_G
Cuts at: 6378 6378
Size: 8779
MluI A'CGCG_T
Cuts at: 8655 8655
Size: 8779
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3968 3976 4925 5283 7893 8077 1305
Size: 2663 8 949 358 2610 184 2007
Fragments arranged by size:
2663 2610 2007 949 358 184 8
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2535 2655 3239 5562 5703
Size: 54 1425 343 703 120 584 2323 141
Cuts at: 5703 10
Size: 3086
Fragments arranged by size:
3086 2323 1425 703 584 343 141 120
54
MslI CAynn'nnrTG
Cuts at: 518 1166 2219 2529 4709 4859 6737 7096 7255
Size: 648 1053 310 2180 150 1878 359 159
Cuts at: 7255 518
Size: 2042
Fragments arranged by size:
2180 2042 1878 1053 648 359 310 159
150
MspA1I CmG'CkG
Cuts at: 1606 2101 2620 2682 2887 3118 6195 6872 8020
Size: 495 519 62 205 231 3077 677 1148
Cuts at: 8020 8265 1606
Size: 245 2120
Fragments arranged by size:
3077 2120 1148 677 519 495 245 231
205 62
NcoI C'CATG_G
Cuts at: 513 1065 2004 4546 4860 513
Size: 552 939 2542 314 4432
Fragments arranged by size:
4432 2542 939 552 314
NdeI CA'TA_TG
Cuts at: 387 387
Size: 8779
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 8779
NotI GC'GGCC_GC
Cuts at: 6226 6226
Size: 8779
NruI TCG'CGA
Cuts at: 3543 3543
Size: 8779
NspI r_CATG'y
Cuts at: 1132 1229 2044 4490 5695 6137 7682 1132
Size: 97 815 2446 1205 442 1545 2229
Fragments arranged by size:
2446 2229 1545 1205 815 442 97
PasI CC'CwG_GG
Cuts at: 1348 2804 3094 3930 4434 5196 5665 1348
Size: 1456 290 836 504 762 469 4462
Fragments arranged by size:
4462 1456 836 762 504 469 290
PciI A'CATG_T
Cuts at: 7678 7678
Size: 8779
PflMI CCAn_nnn'nTGG
Cuts at: 1108 4175 1108
Size: 3067 5712
PfoI T'CCnGG_A
Cuts at: 4652 5124 4652
Size: 472 8307
PmeI GTTT'AAAC
Cuts at: 6083 6083
Size: 8779
PmlI CAC'GTG
Cuts at: 2206 5352 2206
Size: 3146 5633
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5609 5641 6848 6880 8361 8393 5609
Size: 32 1207 32 1481 32 5995
Fragments arranged by size:
5995 1481 1207 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 3388 4212 3388
Size: 824 7955
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 8779
PsiI TTA'TAA
Cuts at: 6349 6349
Size: 8779
PspOMI G'GGCC_C
Cuts at: 2962 2962
Size: 8779
PspXI vC'TCGA_Gb
Cuts at: 3585 3839 3585
Size: 254 8525
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 5300 5332 5300
Size: 32 8747
PstI C_TGCA'G
Cuts at: 830 2409 2531 2723 2789 2909 3663 4920 5334
Size: 1579 122 192 66 120 754 1257 414
Cuts at: 5334 6131 830
Size: 797 3478
Fragments arranged by size:
3478 1579 1257 797 754 414 192 122
120 66
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8779
PvuII CAG'CTG
Cuts at: 2101 2101
Size: 8779
SalI G'TCGA_C
Cuts at: 1936 6121 1936
Size: 4185 4594
SapI GCTCTTCn'nnn_
Cuts at: 2757 2757
Size: 8779
SbfI CC_TGCA'GG
Cuts at: 5334 6131 5334
Size: 797 7982
ScaI AGT'ACT
Cuts at: 7027 8739 7027
Size: 1712 7067
SfcI C'TryA_G
Cuts at: 826 1045 2405 2527 2719 2785 2905 3659 4426
Size: 219 1360 122 192 66 120 754 767
Cuts at: 4426 4916 5330 6127 7262 7943 8134 826
Size: 490 414 797 1135 681 191 1471
Fragments arranged by size:
1471 1360 1135 797 767 754 681 490
414 219 192 191 122 120 66
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8779
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 8779
SmaI CCC'GGG
Cuts at: 1872 2729 6106 1872
Size: 857 3377 4545
Fragments arranged by size:
4545 3377 857
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3258 3585 3839 6883 7784 8046
Size: 197 926 1315 327 254 3044 901 262
Cuts at: 8046 820
Size: 1553
Fragments arranged by size:
3044 1553 1315 926 901 327 262 254
197
SnaBI TAC'GTA
Cuts at: 493 6570 493
Size: 6077 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 8779
SphI G_CATG'C
Cuts at: 1132 4490 6137 1132
Size: 3358 1647 3774
Fragments arranged by size:
3774 3358 1647
SspI AAT'ATT
Cuts at: 5 52 6703 5
Size: 47 6651 2081
Fragments arranged by size:
6651 2081 47
StuI AGG'CCT
Cuts at: 1517 2258 2375 1517
Size: 741 117 7921
Fragments arranged by size:
7921 741 117
StyI C'CwwG_G
Cuts at: 513 1065 2004 2226 3231 4125 4203 4546 4632
Size: 552 939 222 1005 894 78 343 86
Cuts at: 4632 4860 6218 513
Size: 228 1358 3074
Fragments arranged by size:
3074 1358 1005 939 894 552 343 228
222 86 78
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3257 3324 4721 4925 5063 6806 6823
Size: 1255 1677 67 1397 204 138 1743 17
Cuts at: 6823 6976 7161 7606 325
Size: 153 185 445 1498
Fragments arranged by size:
1743 1677 1498 1397 1255 445 204 185
153 138 67 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2208 2304 2676
Size: 274 80 33 51 1443 231 96 372
Cuts at: 2676 3873 3885 4251 4841 5502 5760 7025 8737
Size: 1197 12 366 590 661 258 1265 1712
Cuts at: 8737 96
Size: 138
Fragments arranged by size:
1712 1443 1265 1197 661 590 372 366
274 258 231 138 96 80 51 33
12
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2642 2674 2735 2767 2783 2815 3290 3322 5336
Size: 32 61 32 16 32 475 32 2014
Cuts at: 5336 5368 5379 5411 8739 8771 2642
Size: 32 11 32 3328 32 2650
Fragments arranged by size:
3328 2650 2014 475 61 32 32 32
32 32 32 32 16 11
Tth111I GACn'n_nGTC
Cuts at: 1316 5006 1316
Size: 3690 5089
XbaI T'CTAG_A
Cuts at: 4234 6115 4234
Size: 1881 6898
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2506 3154 3532 6007 2506
Size: 648 378 2475 5278
Fragments arranged by size:
5278 2475 648 378
XhoI C'TCGA_G
Cuts at: 1943 3585 3839 1943
Size: 1642 254 6883
Fragments arranged by size:
6883 1642 254
XmaI C'CCGG_G
Cuts at: 1870 2727 6104 1870
Size: 857 3377 4545
Fragments arranged by size:
4545 3377 857
XmnI GAAnn'nnTTC
Cuts at: 6908 6908
Size: 8779
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 6587 276
Size: 53 83 186 936 5053 2468
Fragments arranged by size:
5053 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI
EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NruI NspI PasI PciI
PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI
PspOMI PspXI PsrI PstI PvuI PvuII SalI SapI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AscI AvrII BaeI BbeI BfrBI BsiWI
BstZ17I EcoICRI EcoRV FalI FseI FspAI KasI NaeI
NarI NgoMIV NsiI PacI RsrII SacI SacII SanDI
SexAI SfiI SfoI SrfI SwaI