Restriction map for
pFN21AE2668
(Linear) (Six-base) MAPSORT of: KIEE2668.seq Check: 9374 from: 1 to: 4074
KIEE2668.orf
With 182 enzymes: SgfI *
January 26, 2017 16:50 ..
AccI GT'mk_AC
Cuts at: 0 1184 4074
Size: 1184 2890
Acc65I G'GTAC_C
Cuts at: 0 835 1968 3968 4074
Size: 835 1133 2000 106
Fragments arranged by size:
2000 1133 835 106
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 49 68 4074
Size: 49 19 4006
Fragments arranged by size:
4006 49 19
AflIII A'CryG_T
Cuts at: 0 200 4074
Size: 200 3874
AleI CACnn'nnGTG
Cuts at: 0 524 2704 4074
Size: 524 2180 1370
Fragments arranged by size:
2180 1370 524
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 3604 3636 4074
Size: 3604 32 438
Fragments arranged by size:
3604 438 32
AlwNI CAG_nnn'CTG
Cuts at: 0 399 408 891 1530 1689 2977 4074
Size: 399 9 483 639 159 1288 1097
Fragments arranged by size:
1288 1097 639 483 399 159 9
ApaI G_GGCC'C
Cuts at: 0 961 4074
Size: 961 3113
ApaLI G'TGCA_C
Cuts at: 0 2845 4074
Size: 2845 1229
ApoI r'AATT_y
Cuts at: 0 2749 3013 4074
Size: 2749 264 1061
Fragments arranged by size:
2749 1061 264
AvaI C'yCGr_G
Cuts at: 0 722 1580 1834 2066 4074
Size: 722 858 254 232 2008
Fragments arranged by size:
2008 858 722 254 232
BanI G'GyrC_C
Cuts at: 0 701 794 835 1116 1968 2730 2922 3091
Size: 701 93 41 281 852 762 192 169
Cuts at: 3091 3968 4074
Size: 877 106
Fragments arranged by size:
877 852 762 701 281 192 169 106
93 41
BanII G_rGCy'C
Cuts at: 0 70 605 731 764 932 961 1310 2564
Size: 70 535 126 33 168 29 349 1254
Cuts at: 2564 2575 2656 4074
Size: 11 81 1418
Fragments arranged by size:
1418 1254 535 349 168 126 81 70
33 29 11
BbsI GAAGACnn'nnnn_
Cuts at: 0 183 287 3275 4074
Size: 183 104 2988 799
Fragments arranged by size:
2988 799 183 104
BbvCI CC'TCA_GC
Cuts at: 0 1207 3802 4074
Size: 1207 2595 272
Fragments arranged by size:
2595 1207 272
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 443 477 4074
Size: 443 34 3597
Fragments arranged by size:
3597 443 34
BclI T'GATC_A
Cuts at: 0 74 4074
Size: 74 4000
BglI GCCn_nnn'nGGC
Cuts at: 0 1231 1622 4074
Size: 1231 391 2452
Fragments arranged by size:
2452 1231 391
BglII A'GATC_T
Cuts at: 0 1962 2226 4074
Size: 1962 264 1848
Fragments arranged by size:
1962 1848 264
BlpI GC'TnA_GC
Cuts at: 0 260 412 969 3478 4074
Size: 260 152 557 2509 596
Fragments arranged by size:
2509 596 557 260 152
Bme1580I G_kGCm'C
Cuts at: 0 221 706 799 961 2735 2849 3171 3417
Size: 221 485 93 162 1774 114 322 246
Cuts at: 3417 4074
Size: 657
Fragments arranged by size:
1774 657 485 322 246 221 162 114
93
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1329 4074
Size: 1329 2745
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 118 451 609 968 1084 1090 1142 1174
Size: 118 333 158 359 116 6 52 32
Cuts at: 1174 2346 2670 2999 3307 4074
Size: 1172 324 329 308 767
Fragments arranged by size:
1172 767 359 333 329 324 308 158
118 116 52 32 6
Bpu10I CC'TnA_GC
Cuts at: 0 1207 1379 1666 3802 4074
Size: 1207 172 287 2136 272
Fragments arranged by size:
2136 1207 287 272 172
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1238 4074
Size: 1238 2836
BsaI GGTCTCn'nnnn_
Cuts at: 0 449 1168 1276 2063 2089 2463 2649 2977
Size: 449 719 108 787 26 374 186 328
Cuts at: 2977 4074
Size: 1097
Fragments arranged by size:
1097 787 719 449 374 328 186 108
26
BsaAI yAC'GTr
Cuts at: 0 201 3347 4074
Size: 201 3146 727
Fragments arranged by size:
3146 727 201
BsaHI Gr'CG_yC
Cuts at: 0 3819 4074
Size: 3819 255
BsaWI w'CCGG_w
Cuts at: 0 698 3572 3911 4074
Size: 698 2874 339 163
Fragments arranged by size:
2874 698 339 163
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 220 250 703 733 1285 1315 3280 3310
Size: 220 30 453 30 552 30 1965 30
Cuts at: 3310 3374 3404 3604 3634 4074
Size: 64 30 200 30 440
Fragments arranged by size:
1965 552 453 440 220 200 64 30
30 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 151 154 228 865 937 1241 2079 2349
Size: 151 3 74 637 72 304 838 270
Cuts at: 2349 2452 4074
Size: 103 1622
Fragments arranged by size:
1622 838 637 304 270 151 103 74
72 3
BseYI C'CCAG_C
Cuts at: 0 615 1387 2397 2772 3416 3627 4074
Size: 615 772 1010 375 644 211 447
Fragments arranged by size:
1010 772 644 615 447 375 211
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 296 1318 1702 2323 4074
Size: 296 1022 384 621 1751
Fragments arranged by size:
1751 1022 621 384 296
BsiHKAI G_wGCw'C
Cuts at: 0 2849 3323 4074
Size: 2849 474 751
Fragments arranged by size:
2849 751 474
BsmI GAATG_Cn'
Cuts at: 0 900 2046 4074
Size: 900 1146 2028
Fragments arranged by size:
2028 1146 900
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1503 4042 4074
Size: 1503 2539 32
Fragments arranged by size:
2539 1503 32
Bsp1286I G_dGCh'C
Cuts at: 0 70 221 605 706 731 764 799 932
Size: 70 151 384 101 25 33 35 133
Cuts at: 932 961 1310 2564 2575 2656 2735 2849 3171
Size: 29 349 1254 11 81 79 114 322
Cuts at: 3171 3323 3417 4074
Size: 152 94 657
Fragments arranged by size:
1254 657 384 349 322 152 151 133
114 101 94 81 79 70 35 33
29 25 11
BspEI T'CCGG_A
Cuts at: 0 3572 4074
Size: 3572 502
BspHI T'CATG_A
Cuts at: 0 1908 2006 4074
Size: 1908 98 2068
Fragments arranged by size:
2068 1908 98
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 3038 4074
Size: 3038 1036
BsrDI GCAATG_nn'
Cuts at: 0 149 2625 4074
Size: 149 2476 1449
Fragments arranged by size:
2476 1449 149
BsrGI T'GTAC_A
Cuts at: 0 203 1880 3755 4074
Size: 203 1677 1875 319
Fragments arranged by size:
1875 1677 319 203
BssSI C'ACGA_G
Cuts at: 0 322 4074
Size: 322 3752
BstAPI GCAn_nnn'nTGC
Cuts at: 0 408 593 1116 1689 2025 4074
Size: 408 185 523 573 336 2049
Fragments arranged by size:
2049 573 523 408 336 185
BstEII G'GTnAC_C
Cuts at: 0 102 333 4074
Size: 102 231 3741
Fragments arranged by size:
3741 231 102
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1029 2706 4074
Size: 1029 1677 1368
Fragments arranged by size:
1677 1368 1029
BstYI r'GATC_y
Cuts at: 0 1526 1962 2226 2871 4074
Size: 1526 436 264 645 1203
Fragments arranged by size:
1526 1203 645 436 264
Bsu36I CC'TnA_GG
Cuts at: 0 240 4074
Size: 240 3834
BtgI C'CryG_G
Cuts at: 0 531 1289 2541 2855 4074
Size: 531 758 1252 314 1219
Fragments arranged by size:
1252 1219 758 531 314
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1406 1553 4074
Size: 1406 147 2521
Fragments arranged by size:
2521 1406 147
BtsI GCAGTG_nn'
Cuts at: 0 519 531 892 909 1058 1220 1306 2261
Size: 519 12 361 17 149 162 86 955
Cuts at: 2261 2920 3785 3863 4074
Size: 659 865 78 211
Fragments arranged by size:
955 865 659 519 361 211 162 149
86 78 17 12
ClaI AT'CG_AT
Cuts at: 0 1781 4074
Size: 1781 2293
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1491 1526 2109 2144 2571 2606 4074
Size: 1491 35 583 35 427 35 1468
Fragments arranged by size:
1491 1468 583 427 35 35 35
DraIII CAC_nnn'GTG
Cuts at: 0 3347 4074
Size: 3347 727
EaeI y'GGCC_r
Cuts at: 0 528 648 876 1232 1490 3555 3696 4074
Size: 528 120 228 356 258 2065 141 378
Fragments arranged by size:
2065 528 378 356 258 228 141 120
EarI CTCTTCn'nnn_
Cuts at: 0 752 938 2438 4074
Size: 752 186 1500 1636
Fragments arranged by size:
1636 1500 752 186
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 49 68 118 451 609 968 1084 1090
Size: 49 19 50 333 158 359 116 6
Cuts at: 1090 1142 1174 2346 2670 2999 3307 4074
Size: 52 32 1172 324 329 308 767
Fragments arranged by size:
1172 767 359 333 329 324 308 158
116 52 50 49 32 19 6
EcoNI CCTnn'n_nnAGG
Cuts at: 0 3723 4074
Size: 3723 351
EcoO109I rG'GnC_Cy
Cuts at: 0 957 958 1095 1383 1686 2207 3291 4074
Size: 957 1 137 288 303 521 1084 783
Fragments arranged by size:
1084 957 783 521 303 288 137 1
EcoRI G'AATT_C
Cuts at: 0 2749 4074
Size: 2749 1325
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 703 735 1384 1416 1563 1574 1595 1606
Size: 703 32 649 32 147 11 21 11
Cuts at: 1606 2194 2226 2972 3004 3715 3747 4074
Size: 588 32 746 32 711 32 327
Fragments arranged by size:
746 711 703 649 588 327 147 32
32 32 32 32 21 11 11
HincII GTy'rAC
Cuts at: 0 2997 3027 4074
Size: 2997 30 1047
Fragments arranged by size:
2997 1047 30
KpnI G_GTAC'C
Cuts at: 0 839 1972 3972 4074
Size: 839 1133 2000 102
Fragments arranged by size:
2000 1133 839 102
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1963 1971 2920 3278 4074
Size: 1963 8 949 358 796
Fragments arranged by size:
1963 949 796 358 8
MscI TGG'CCA
Cuts at: 0 530 650 1234 3557 3698 4074
Size: 530 120 584 2323 141 376
Fragments arranged by size:
2323 584 530 376 141 120
MslI CAynn'nnrTG
Cuts at: 0 214 524 2704 2854 4074
Size: 214 310 2180 150 1220
Fragments arranged by size:
2180 1220 310 214 150
MspA1I CmG'CkG
Cuts at: 0 96 615 677 882 1113 4074
Size: 96 519 62 205 231 2961
Fragments arranged by size:
2961 519 231 205 96 62
NcoI C'CATG_G
Cuts at: 0 2541 2855 4074
Size: 2541 314 1219
Fragments arranged by size:
2541 1219 314
NruI TCG'CGA
Cuts at: 0 1538 4074
Size: 1538 2536
NspI r_CATG'y
Cuts at: 0 39 2485 3690 4074
Size: 39 2446 1205 384
Fragments arranged by size:
2446 1205 384 39
PasI CC'CwG_GG
Cuts at: 0 799 1089 1925 2429 3191 3660 4074
Size: 799 290 836 504 762 469 414
Fragments arranged by size:
836 799 762 504 469 414 290
PflMI CCAn_nnn'nTGG
Cuts at: 0 2170 4074
Size: 2170 1904
PfoI T'CCnGG_A
Cuts at: 0 2647 3119 4074
Size: 2647 472 955
Fragments arranged by size:
2647 955 472
PmlI CAC'GTG
Cuts at: 0 201 3347 4074
Size: 201 3146 727
Fragments arranged by size:
3146 727 201
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 3604 3636 4074
Size: 3604 32 438
Fragments arranged by size:
3604 438 32
PpuMI rG'GwC_Cy
Cuts at: 0 1383 2207 4074
Size: 1383 824 1867
Fragments arranged by size:
1867 1383 824
PspOMI G'GGCC_C
Cuts at: 0 957 4074
Size: 957 3117
PspXI vC'TCGA_Gb
Cuts at: 0 1580 1834 4074
Size: 1580 254 2240
Fragments arranged by size:
2240 1580 254
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 3295 3327 4074
Size: 3295 32 747
Fragments arranged by size:
3295 747 32
PstI C_TGCA'G
Cuts at: 0 404 526 718 784 904 1658 2915 3329
Size: 404 122 192 66 120 754 1257 414
Cuts at: 3329 4074
Size: 745
Fragments arranged by size:
1257 754 745 414 404 192 122 120
66
PvuII CAG'CTG
Cuts at: 0 96 4074
Size: 96 3978
SapI GCTCTTCn'nnn_
Cuts at: 0 752 4074
Size: 752 3322
SbfI CC_TGCA'GG
Cuts at: 0 3329 4074
Size: 3329 745
SfcI C'TryA_G
Cuts at: 0 400 522 714 780 900 1654 2421 2911
Size: 400 122 192 66 120 754 767 490
Cuts at: 2911 3325 4074
Size: 414 749
Fragments arranged by size:
767 754 749 490 414 400 192 122
120 66
SmaI CCC'GGG
Cuts at: 0 724 4074
Size: 724 3350
SmlI C'TyrA_G
Cuts at: 0 1253 1580 1834 4074
Size: 1253 327 254 2240
Fragments arranged by size:
2240 1253 327 254
SphI G_CATG'C
Cuts at: 0 2485 4074
Size: 2485 1589
StuI AGG'CCT
Cuts at: 0 253 370 4074
Size: 253 117 3704
Fragments arranged by size:
3704 253 117
StyI C'CwwG_G
Cuts at: 0 221 1226 2120 2198 2541 2627 2855 4074
Size: 221 1005 894 78 343 86 228 1219
Fragments arranged by size:
1219 1005 894 343 228 221 86 78
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1252 1319 2716 2920 3058 4074
Size: 1252 67 1397 204 138 1016
Fragments arranged by size:
1397 1252 1016 204 138 67
TatI w'GTAC_w
Cuts at: 0 203 299 671 1868 1880 2246 2836 3497
Size: 203 96 372 1197 12 366 590 661
Cuts at: 3497 3755 4074
Size: 258 319
Fragments arranged by size:
1197 661 590 372 366 319 258 203
96 12
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 637 669 730 762 778 810 1285 1317
Size: 637 32 61 32 16 32 475 32
Cuts at: 1317 3331 3363 3374 3406 4074
Size: 2014 32 11 32 668
Fragments arranged by size:
2014 668 637 475 61 32 32 32
32 32 32 16 11
Tth111I GACn'n_nGTC
Cuts at: 0 3001 4074
Size: 3001 1073
XbaI T'CTAG_A
Cuts at: 0 2229 4074
Size: 2229 1845
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 501 1149 1527 4002 4074
Size: 501 648 378 2475 72
Fragments arranged by size:
2475 648 501 378 72
XhoI C'TCGA_G
Cuts at: 0 1580 1834 4074
Size: 1580 254 2240
Fragments arranged by size:
2240 1580 254
XmaI C'CCGG_G
Cuts at: 0 722 4074
Size: 722 3352
Enzymes that do cut:
AccI Acc65I AcuI AflIII AleI AloI AlwNI ApaI
ApaLI ApoI AvaI BanI BanII BbsI BbvCI BcgI
BclI BglI BglII BlpI Bme1580I BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrDI BsrGI BssSI BstAPI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraIII EaeI EarI
Eco57MI EcoNI EcoO109I EcoRI Hin4I HincII KpnI MmeI
MscI MslI MspA1I NcoI NruI NspI PasI PflMI
PfoI PmlI PpiI PpuMI PspOMI PspXI PsrI PstI
PvuII SapI SbfI SfcI SmaI SmlI SphI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI
Enzymes that do not cut:
AarI AatII AclI AfeI AflII AgeI AhdI AlfI
AscI AseI AsiSI AvrII BaeI BamHI BbeI BciVI
BfrBI BmgBI BmrI BmtI BsaBI BsiEI BsiWI BsrBI
BsrFI BssHII BstBI BstZ17I DraI DrdI EagI EciI
EcoICRI EcoRV FalI FseI FspI FspAI HaeII HindIII
HpaI KasI MfeI MluI NaeI NarI NdeI NgoMIV
NheI NotI NsiI PacI PciI PmeI PshAI PsiI
PvuI RsrII SacI SacII SalI SanDI ScaI SexAI
SfiI SfoI SgfI SgrAI SnaBI SpeI SrfI SspI
SwaI XmnI ZraI