################################## # NO CUT ENZYMES ( 190 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AciI AclI AcyI AflII AflIII AgeI AhdI Alw44I AocI Aor51HI ApaI ApaLI AscI Asp700I Asp718I AspEI AspHI AspI AvaI AvaII AviII AvrII BalI BamHI BanI BanII BbeI BbrPI BbsI BbvCI BcgI BciVI BclI BcnI BfrI BglI BlnI BlpI BmyI Bpu1102I BpuAI BsaBI BsaHI BsaI BsaWI BseAI BseRI BsiEI BsiHKAI BsiWI BsmFI BsmI BsoBI Bsp1286I Bsp1407I BspDI BspEI BspHI BspLU11I BsrBI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstZ17I Bsu36I CelII CfoI Cfr10I Cfr13I ClaI CpoI DraI DraII DrdI DsaI EaeI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoO109I EcoO65I EcoRI EcoRV EcoT14I EcoT22I FauI FbaI FseI FspI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HincII HindII HindIII HpaI HpaII KasI KpnI KspI MamI MfeI MluI MluNI MroI MscI MspI MunI MvnI NaeI NarI NciI NcoI NdeI NgoMIV NheI NlaIV NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PleI PmaCI PmeI PmlI PpuMI PshAI Psp1406I PstI PvuI RcaI RsrII SacI SacII SalI Sau96I SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI StyI SwaI TaqI Tsp45I Tth111I TthHB8I Van91I XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 31 enzymes) # ################################### AfaI 73 GTAC GT^AC AlwNI 179 CAGCTGCTG CAG[ACGT][ACGT][ACGT]^CTG AseI 628 ATTAAT AT^TAAT AsnI 628 ATTAAT AT^TAAT BbvI 181 GCTGC GCAGC(8/12) BglII 24 AGATCT A^GATCT BpmI 281 CTGGAG CTGGAG(16/14) BsaAI 74 TACGTG [CT]AC^GT[GA] BsgI 310 GTGCAG GTGCAG(16/14) BsmBI 605 GAGACG CGTCTC(1/5) BspMI 312 GCAGGT ACCTGC(4/8) BsrI 280 ACTGG ACTGG(1/-1) BstF5I 235 GGATG GGATG(2/0) BstXI 404 CCATCTCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Cac8I 660 GCAAGC GC[ACGT]^[ACGT]GC DdeI 302 CTGAG C^T[ACGT]AG DraIII 480 CACAAGGTG CAC[ACGT][ACGT][ACGT]^GTG EcoNI 495 CCTGACCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG Fnu4HI 181 GCTGC GC^[ACGT]GC FokI 235 GGATG GGATG(9/13) HphI 216 GGTGA GGTGA(8/7) ItaI 181 GCTGC GC^[ACGT]GC MaeII 75 ACGT A^CGT MaeIII 371 GTTAC ^GT[ACGT]AC NlaIII 378 CATG CATG^ PshBI 628 ATTAAT AT^TAAT RsaI 73 GTAC GT^AC SapI 10 GAAGAGC GCTCTTC(1/4) SfcI 16 CTACAG C^T[GA][CT]AG TseI 181 GCTGC G^C[AT]GC XbaI 27 TCTAGA T^CTAGA ################################### # TWO CUT ENZYMES ( 24 enzymes) # ################################### AcsI 590 AAATTT [GA]^AATT[CT] 679 GAATTT AlwI 556 GATCC GGATC(4/5) 240 GGATC ApoI 590 AAATTT [GA]^AATT[CT] 679 GAATTT BsaJI 500 CCCAGG C^C[ACGT][ACGT]GG 638 CCTGGG BsiYI 495 CCTGACCCAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 559 CCTAGTGAGGG BslI 495 CCTGACCCAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 559 CCTAGTGAGGG BssKI 501 CCAGG ^CC[ACGT]GG 638 CCTGG BstNI 501 CCAGG CC^[AT]GG 638 CCTGG BstYI 24 AGATCT [GA]^GATC[CT] 555 AGATCC EcoRII 501 CCAGG ^CC[AT]GG 638 CCTGG HinfI 354 GAATC G^A[ACGT]TC 646 GAATC MflI 24 AGATCT [GA]^GATC[CT] 555 AGATCC MseI 277 TTAA T^TAA 629 TTAA MslI 378 CATGATTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 405 CATCTCTGTG MspA1I 89 CAGCTG C[AC]G^C[GT]G 179 CAGCTG MvaI 501 CCAGG CC^[AT]GG 638 CCTGG MwoI 82 GCCCTTACAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC 184 GCTGTGGTTGC PvuII 89 CAGCTG CAG^CTG 179 CAGCTG ScrFI 501 CCAGG CC^[ACGT]GG 638 CCTGG SfaNI 79 GATGC GCATC(5/9) 129 GATGC TfiI 354 GAATC G^A[AT]TC 646 GAATC Tru9I 277 TTAA T^TAA 629 TTAA TspRI 300 CACTG CA[GC]TG[ACGT][ACGT]^ 546 CACTG XhoII 24 AGATCT [GA]^GATC[CT] 555 AGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 13 enzymes) # ######################################### AluI BfaI BsmAI DpnII EarI Ksp632I MaeI MboI MboII MnlI NdeII Sau3AI Tsp509I