(Linear) (Six-base) MAPSORT of: KIEE3883.seq  Check: 584  from: 1  to: 684



KIEE3883.orf

 With 182 enzymes: SgfI * 

                        August 4, 2021 20:20  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     303     684 
   Size:        303     381
 
AlwNI CAG_nnn'CTG

Cuts at:      0     184     684 
   Size:        184     500
 
ApoI r'AATT_y

Cuts at:      0     590     679     684 
   Size:        590      89       5

  Fragments arranged by size: 

                 590      89       5
 
AseI AT'TA_AT

Cuts at:      0     629     684 
   Size:        629      55
 
BglII A'GATC_T

Cuts at:      0      24     684 
   Size:         24     660
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     552     684 
   Size:        552     132
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     302     684 
   Size:        302     382
 
BsaAI yAC'GTr

Cuts at:      0      76     684 
   Size:         76     608
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     331     684 
   Size:        331     353
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     599     684 
   Size:        599      85
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     303     684 
   Size:        303     381
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     411     684 
   Size:        411     273
 
BstYI r'GATC_y

Cuts at:      0      24     555     684 
   Size:         24     531     129

  Fragments arranged by size: 

                 531     129      24
 
DraIII CAC_nnn'GTG

Cuts at:      0     485     684 
   Size:        485     199
 
EarI CTCTTCn'nnn_

Cuts at:      0       5      26     361     684 
   Size:          5      21     335     323

  Fragments arranged by size: 

                 335     323      21       5
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     302     684 
   Size:        302     382
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     499     684 
   Size:        499     185
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     175     684 
   Size:        175     509
 
MslI CAynn'nnrTG

Cuts at:      0     382     409     684 
   Size:        382      27     275

  Fragments arranged by size: 

                 382     275      27
 
MspA1I CmG'CkG

Cuts at:      0      91     181     684 
   Size:         91      90     503

  Fragments arranged by size: 

                 503      91      90
 
PvuII CAG'CTG

Cuts at:      0      91     181     684 
   Size:         91      90     503

  Fragments arranged by size: 

                 503      91      90
 
SapI GCTCTTCn'nnn_

Cuts at:      0       5     684 
   Size:          5     679
 
SfcI C'TryA_G

Cuts at:      0      16     684 
   Size:         16     668
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     630     684 
   Size:        630      54
 
XbaI T'CTAG_A

Cuts at:      0      27     684 
   Size:         27     657
 

 Enzymes that do cut:

     AarI    AlwNI     ApoI     AseI    BglII     BplI     BpmI    BsaAI
     BsgI    BsmBI    BspMI    BstXI    BstYI   DraIII     EarI  Eco57MI
    EcoNI     FalI     MslI   MspA1I    PvuII     SapI     SfcI    TaqII
     XbaI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI     ApaI    ApaLI     AscI
    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI    BanII     BbeI
     BbsI    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI     BlpI
 Bme1580I    BmgBI     BmrI     BmtI   Bpu10I    BpuEI     BsaI    BsaBI
    BsaHI    BsaWI    BsaXI    BseRI    BseYI    BsiEI  BsiHKAI    BsiWI
     BsmI Bsp1286I    BspEI    BspHI    BsrBI    BsrDI    BsrFI    BsrGI
   BssHII    BssSI   BstAPI    BstBI   BstEII  BstZ17I   Bsu36I     BtgI
    BtgZI     BtsI     ClaI    CspCI     DraI     DrdI     EaeI     EagI
     EciI  EcoICRI EcoO109I    EcoRI    EcoRV     FseI     FspI    FspAI
    HaeII    Hin4I   HincII  HindIII     HpaI     KasI     KpnI     MfeI
     MluI     MmeI     MscI     NaeI     NarI     NcoI     NdeI   NgoMIV
     NheI     NotI     NruI     NsiI     NspI     PacI     PasI     PciI
    PflMI     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI
   PspOMI    PspXI     PsrI     PstI     PvuI    RsrII     SacI    SacII
     SalI    SanDI     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI
    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI     SspI
     StuI     StyI     SwaI     TatI     TstI  Tth111I     XcmI     XhoI
     XmaI     XmnI     ZraI