Restriction map for
pFN21AE5779
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# NO CUT ENZYMES ( 118 enzymes) #
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AatII Acc65I AccII AclI AcyI
AflII AgeI AhdI Alw44I Aor51HI
ApaI ApaLI AscI Asp700I Asp718I
AspEI AspI AvaI AviII AvrII
BbeI BbrPI BbsI BcgI BclI
BfrI BglI BglII BlnI BpuAI
BsaAI BsaHI BsgI BsiEI BsiWI
BsoBI BspDI BsrBI BsrFI BssHII
BssSI Bst1107I BstAPI BstEII BstPI
BstUI BstZ17I CfoI Cfr10I ClaI
CpoI DrdI EagI Eam1105I EclXI
Eco47III Eco52I EcoO65I EcoT22I FbaI
FseI FspI HaeII HgaI HhaI
Hin1I HinP1I HincII HindII HindIII
HpaI KasI KpnI KspI MaeII
MfeI MluI MunI MvnI NaeI
NarI NgoMIV NheI NotI NruI
NsiI PacI PaeR7I PflFI PflMI
PinAI PmaCI PmeI PmlI Psp1406I
PvuI RsrII SacII SalI ScaI
SexAI SfiI SfoI SgrAI SmaI
SmlI SnaBI SpeI SspI StuI
SwaI Tth111I Van91I XcmI XhoI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 51 enzymes) #
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AccI 862 GTAGAC GT^[AC][GT]AC
AccIII 146 TCCGGA T^CCGGA
AflIII 157 ACATGT A^C[GA][CT]GT
AseI 1275 ATTAAT AT^TAAT
AsnI 1275 ATTAAT AT^TAAT
BalI 974 TGGCCA TGG^CCA
BamHI 875 GGATCC G^GATCC
BbvCI 964 GCTGAGG CCTCAGC(-5/-2)
BciVI 1112 GTATCC GTATCC(6/5)
BcnI 1824 CCGGG CC^[GC]GG
BfaI 1387 CTAG C^TAG
BlpI 436 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 436 GCTGAGC GC^T[ACGT]AGC
BsaBI 414 GATTGAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 1291 GAGACC GGTCTC(1/5)
BsaWI 146 TCCGGA [AT]^CCGG[AT]
BseAI 146 TCCGGA T^CCGGA
BsmBI 176 CGTCTC CGTCTC(1/5)
BsmI 447 GAATGC GAATGC(1/-1)
BspEI 146 TCCGGA T^CCGGA
BspLU11I 157 ACATGT A^CATGT
BspMI 557 ACCTGC ACCTGC(4/8)
BsrDI 1598 GCAATG GCAATG(2/0)
BstBI 1622 TTCGAA TT^CGAA
BstXI 1481 CCAGAGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 436 GCTGAGC GC^T[ACGT]AGC
DraI 1590 TTTAAA TTT^AAA
DraIII 198 CACCTTGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 1089 CCATGG C^C[GA][CT]GG
EaeI 974 TGGCCA [CT]^GGCC[GA]
EcoRV 1027 GATATC GAT^ATC
FauI 591 GCGGG CCCGC(4/6)
MaeI 1387 CTAG C^TAG
MamI 414 GATTGAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 974 TGGCCA TGG^CCA
MroI 146 TCCGGA T^CCGGA
MscI 974 TGGCCA TGG^CCA
MwoI 1921 GCAGAAAGAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NciI 1824 CCGGG CC^[GC]GG
NcoI 1089 CCATGG C^CATGG
NdeI 1107 CATATG CA^TATG
NspV 1622 TTCGAA TT^CGAA
PshAI 563 GACTGAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 1275 ATTAAT AT^TAAT
SacI 1928 GAGCTC GAGCT^C
SapI 502 GCTCTTC GCTCTTC(1/4)
SbfI 376 CCTGCAGG CCTGCA^GG
SfuI 1622 TTCGAA TT^CGAA
SphI 1342 GCATGC GCATG^C
Sse8387I 376 CCTGCAGG CCTGCA^GG
XbaI 1386 TCTAGA T^CTAGA
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# TWO CUT ENZYMES ( 28 enzymes) #
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AciI 591 GCGG CCGC(-3/-1)
758 GCGG
AlwNI 299 CAGCCTCTG CAG[ACGT][ACGT][ACGT]^CTG
1853 CAGATTCTG
AocI 1745 CCTTAGG CC^T[ACGT]AGG
1867 CCTGAGG
BanI 664 GGTGCC G^G[CT][GA]CC
1722 GGCACC
BanII 1552 GAGCCC G[GA]GC[CT]^C
1928 GAGCTC
BseRI 114 GAGGAG GAGGAG(10/8)
1397 GAGGAG
Bsp1407I 29 TGTACA T^GTACA
1544 TGTACA
BspHI 581 TCATGA T^CATGA
1838 TCATGA
BsrGI 29 TGTACA T^GTACA
1544 TGTACA
Bsu36I 1745 CCTTAGG CC^T[ACGT]AGG
1867 CCTGAGG
DraII 568 AGGTCCC [GA]G^G[ACGT]CC[CT]
652 AGGACCC
EarI 503 CTCTTC CTCTTC(1/4)
1456 CTCTTC
Eco81I 1745 CCTTAGG CC^T[ACGT]AGG
1867 CCTGAGG
EcoNI 1461 CCTGCCCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1729 CCTCATGGAGG
EcoO109I 568 AGGTCCC [GA]G^G[ACGT]CC[CT]
652 AGGACCC
EcoT14I 1089 CCATGG C^C[AT][AT]GG
1466 CCAAGG
HapII 147 CCGG C^CGG
1824 CCGG
HpaII 147 CCGG C^CGG
1824 CCGG
HphI 409 GGTGA GGTGA(8/7)
197 TCACC
Ksp632I 503 CTCTTC CTCTTC(1/4)
1456 CTCTTC
MspI 147 CCGG C^CGG
1824 CCGG
NspI 157 ACATGT [GA]CATG^[CT]
1342 GCATGC
PpuMI 568 AGGTCCC [GA]G^G[AT]CC[CT]
652 AGGACCC
PstI 377 CTGCAG CTGCA^G
1642 CTGCAG
RcaI 581 TCATGA T^CATGA
1838 TCATGA
SfaNI 1072 GATGC GCATC(5/9)
1790 GCATC
SspBI 29 TGTACA T^GTACA
1544 TGTACA
StyI 1089 CCATGG C^C[AT][AT]GG
1466 CCAAGG
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# 3 OR MORE CUT ENZYMES ( 61 enzymes) #
#########################################
AcsI AfaI AluI AlwI ApoI
AspHI AvaII BbvI BmyI BpmI
BsaJI BsiHKAI BsiYI BslI BsmAI
BsmFI Bsp1286I BsrI BssKI BstF5I
BstNI BstYI Cac8I Cfr13I DdeI
DpnII EcoRI EcoRII Fnu4HI FokI
HaeIII HinfI ItaI MaeIII MboI
MboII MflI MnlI MseI MslI
MspA1I MvaI NdeII NlaIII NlaIV
PleI PvuII RsaI Sau3AI Sau96I
ScrFI SfcI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XhoII