(Circular) (Six-base) MAPSORT of: pFN21AE5779.seq Check: 6703 from: 1 to: 6640 pFN21AE5779 6640 bp With 182 enzymes: SgfI * December 8, 2014 13:44 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4450 278 Size: 53 83 186 936 2914 2468 Fragments arranged by size: 2914 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2868 3983 1937 Size: 931 1115 4594 Fragments arranged by size: 4594 1115 931 Acc65I G'GTAC_C Cuts at: 3961 3961 Size: 6640 AclI AA'CG_TT Cuts at: 1563 4767 5140 1563 Size: 3204 373 3063 Fragments arranged by size: 3204 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3144 3902 4703 6087 6355 1632 Size: 1512 758 801 1384 268 1917 Fragments arranged by size: 1917 1512 1384 801 758 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6443 AflIII A'CryG_T Cuts at: 1203 2162 5539 6516 1203 Size: 959 3377 977 1327 Fragments arranged by size: 3377 1327 977 959 AgeI A'CCGG_T Cuts at: 5471 5471 Size: 6640 AhdI GACnn_n'nnGTC Cuts at: 5369 5369 Size: 6640 AleI CACnn'nnGTG Cuts at: 1166 3531 1166 Size: 2365 4275 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2483 1184 Size: 1299 5341 AlwNI CAG_nnn'CTG Cuts at: 2309 3863 4383 5955 2309 Size: 1554 520 1572 2994 Fragments arranged by size: 2994 1572 1554 520 ApaLI G'TGCA_C Cuts at: 4697 5853 4697 Size: 1156 5484 ApoI r'AATT_y Cuts at: 1493 2124 2132 3235 3630 3949 4175 4327 6436 Size: 631 8 1103 395 319 226 152 2109 Cuts at: 6436 1493 Size: 1697 Fragments arranged by size: 2109 1697 1103 631 395 319 226 152 8 AseI AT'TA_AT Cuts at: 160 1032 3281 5194 160 Size: 872 2249 1913 1606 Fragments arranged by size: 2249 1913 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6640 AvaI C'yCGr_G Cuts at: 1870 1943 3965 1870 Size: 73 2022 4545 Fragments arranged by size: 4545 2022 73 BamHI G'GATC_C Cuts at: 2880 3970 4337 2880 Size: 1090 367 5183 Fragments arranged by size: 5183 1090 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2669 3727 3961 5416 618 Size: 325 217 627 882 1058 234 1455 1842 Fragments arranged by size: 1842 1455 1058 882 627 325 234 217 BanII G_rGCy'C Cuts at: 1391 3561 3937 3959 1391 Size: 2170 376 22 4072 Fragments arranged by size: 4072 2170 376 22 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5669 BbvCI CC'TCA_GC Cuts at: 2970 2970 Size: 6640 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4831 4865 1903 Size: 34 2894 34 3678 Fragments arranged by size: 3678 2894 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3128 4532 5742 3128 Size: 1404 1210 4026 Fragments arranged by size: 4026 1404 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 6640 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5251 136 Size: 107 122 71 631 605 3579 1525 Fragments arranged by size: 3579 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6635 6635 Size: 6640 BlpI GC'TnA_GC Cuts at: 2442 4057 2442 Size: 1615 5025 Bme1580I G_kGCm'C Cuts at: 1790 3730 4701 5857 1790 Size: 1940 971 1156 2573 Fragments arranged by size: 2573 1940 1156 971 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6640 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2886 3525 4371 Size: 416 53 83 399 24 1469 639 846 Cuts at: 4371 5329 442 Size: 958 1753 Fragments arranged by size: 1753 1469 958 846 639 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6640 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3428 3428 Size: 6640 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2891 3225 3351 3692 5300 1239 Size: 1652 334 126 341 1608 2579 Fragments arranged by size: 2579 1652 1608 341 334 126 Bpu10I CC'TnA_GC Cuts at: 2970 2970 Size: 6640 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4765 5630 5928 4765 Size: 865 298 5477 Fragments arranged by size: 5477 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3290 5303 882 Size: 617 1791 2013 2219 Fragments arranged by size: 2219 2013 1791 617 BsaAI yAC'GTr Cuts at: 493 1204 4431 493 Size: 711 3227 2702 Fragments arranged by size: 3227 2702 711 BsaBI GATnn'nnATC Cuts at: 2423 6634 2423 Size: 4211 2429 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4447 4829 5464 275 Size: 53 83 186 936 2914 382 635 1451 Fragments arranged by size: 2914 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2151 5073 5471 5745 5892 6303 2151 Size: 2922 398 274 147 411 2488 Fragments arranged by size: 2922 2488 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3772 3802 1178 Size: 30 165 30 2369 30 4016 Fragments arranged by size: 4016 2369 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2134 3417 1186 Size: 948 1283 4409 Fragments arranged by size: 4409 1283 948 BseYI C'CCAG_C Cuts at: 5843 6339 5843 Size: 496 6144 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 6067 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4090 4851 5879 1540 Size: 275 186 2089 761 1028 2301 Fragments arranged by size: 2301 2089 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3339 3414 3937 4701 4786 5857 3339 Size: 75 523 764 85 1071 4122 Fragments arranged by size: 4122 1071 764 523 85 75 BsmI GAATG_Cn' Cuts at: 2458 4151 4244 2458 Size: 1693 93 4854 Fragments arranged by size: 4854 1693 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2187 2187 Size: 6640 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3339 3414 3561 3730 3937 3959 4701 Size: 399 1549 75 147 169 207 22 742 Cuts at: 4701 4786 5857 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1549 1071 742 399 207 169 147 85 75 22 BspEI T'CCGG_A Cuts at: 2151 6303 2151 Size: 4152 2488 BspHI T'CATG_A Cuts at: 2586 3843 4529 6259 2586 Size: 1257 686 1730 2967 Fragments arranged by size: 2967 1730 1257 686 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2571 3995 844 Size: 1727 1424 3489 Fragments arranged by size: 3489 1727 1424 BsrBI CCG'CTC Cuts at: 1394 1823 4527 1394 Size: 429 2704 3507 Fragments arranged by size: 3507 2704 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3610 5135 5309 65 Size: 1183 2362 1525 174 1396 Fragments arranged by size: 2362 1525 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5284 5471 1700 Size: 110 3474 187 2869 Fragments arranged by size: 3474 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 2034 3549 96 Size: 1938 1515 3187 Fragments arranged by size: 3187 1938 1515 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6640 BssSI C'ACGA_G Cuts at: 4700 5712 4700 Size: 1012 5628 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6640 BstBI TT'CG_AA Cuts at: 3628 5444 3628 Size: 1816 4824 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6640 BstXI CCAn_nnnn'nTGG Cuts at: 3493 3493 Size: 6640 BstYI r'GATC_y Cuts at: 1972 2880 2932 3785 3970 4337 4722 4739 6191 Size: 908 52 853 185 367 385 17 1452 Cuts at: 6191 6277 6289 6635 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 908 853 385 367 346 185 86 52 17 12 Bsu36I CC'TnA_GG Cuts at: 3751 3873 3751 Size: 122 6518 BtgI C'CryG_G Cuts at: 513 1065 3094 513 Size: 552 2029 4059 Fragments arranged by size: 4059 2029 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5901 Fragments arranged by size: 5901 683 56 BtsI GCAGTG_nn' Cuts at: 4156 4950 4970 4156 Size: 794 20 5826 Fragments arranged by size: 5826 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6640 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2146 2181 3387 3422 529 Size: 35 1582 35 1206 35 3747 Fragments arranged by size: 3747 1582 1206 35 35 35 DraI TTT'AAA Cuts at: 3597 3944 3597 Size: 347 6293 DraIII CAC_nnn'GTG Cuts at: 2208 2208 Size: 6640 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5647 809 Size: 507 4331 1802 Fragments arranged by size: 4331 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2979 4087 4976 Size: 54 1425 50 275 18 1149 1108 889 Cuts at: 4976 6605 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1149 1108 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4087 1537 Size: 275 2275 4090 Fragments arranged by size: 4090 2275 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2514 3467 4570 1353 Size: 1161 953 1103 3423 Fragments arranged by size: 3423 1161 1103 953 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5227 5601 5747 387 Size: 1064 138 3638 374 146 1280 Fragments arranged by size: 3638 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3935 3935 Size: 6640 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2891 3144 3225 3351 3692 3902 4703 Size: 393 1259 253 81 126 341 210 801 Cuts at: 4703 5300 6087 6355 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 1259 801 787 597 393 341 268 253 210 126 81 EcoNI CCTnn'n_nnAGG Cuts at: 3470 3738 3470 Size: 268 6372 EcoO109I rG'GnC_Cy Cuts at: 2574 2658 2574 Size: 84 6556 EcoRI G'AATT_C Cuts at: 2124 2132 3235 3630 3949 4327 2124 Size: 8 1103 395 319 378 4437 Fragments arranged by size: 4437 1103 395 378 319 8 EcoRV GAT'ATC Cuts at: 3034 3034 Size: 6640 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2488 3098 2488 Size: 610 6030 FspI TGC'GCA Cuts at: 5146 5146 Size: 6640 HaeII r_GCGC'y Cuts at: 1678 5787 1678 Size: 4109 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3143 3175 3482 3514 3825 Size: 32 135 32 1571 32 307 32 311 Cuts at: 3825 3857 5281 5313 5355 5387 1373 Size: 32 1424 32 42 32 2626 Fragments arranged by size: 2626 1571 1424 311 307 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3984 4230 669 Size: 988 281 2046 246 3079 Fragments arranged by size: 3079 2046 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6640 HpaI GTT'AAC Cuts at: 4230 4230 Size: 6640 KpnI G_GTAC'C Cuts at: 3965 3965 Size: 6640 MfeI C'AATT_G Cuts at: 4239 4239 Size: 6640 MluI A'CGCG_T Cuts at: 6516 6516 Size: 6640 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5754 5938 1305 Size: 4449 184 2007 Fragments arranged by size: 4449 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2981 10 Size: 54 1425 343 1149 3669 Fragments arranged by size: 3669 1425 1149 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2591 2855 3531 4598 4957 5116 518 Size: 648 1425 264 676 1067 359 159 2042 Fragments arranged by size: 2042 1425 1067 676 648 359 264 159 MspA1I CmG'CkG Cuts at: 1606 2032 2441 3179 4056 4733 5881 6126 1606 Size: 426 409 738 877 677 1148 245 2120 Fragments arranged by size: 2120 1148 877 738 677 426 409 245 NcoI C'CATG_G Cuts at: 513 1065 3094 513 Size: 552 2029 4059 Fragments arranged by size: 4059 2029 552 NdeI CA'TA_TG Cuts at: 387 3113 387 Size: 2726 3914 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6640 NotI GC'GGCC_GC Cuts at: 4087 4087 Size: 6640 NspI r_CATG'y Cuts at: 1132 1229 2166 3351 3998 5543 1132 Size: 97 937 1185 647 1545 2229 Fragments arranged by size: 2229 1545 1185 937 647 97 PasI CC'CwG_GG Cuts at: 1348 2663 1348 Size: 1315 5325 PciI A'CATG_T Cuts at: 2162 5539 2162 Size: 3377 3263 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6640 PmeI GTTT'AAAC Cuts at: 3944 3944 Size: 6640 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4709 4741 6222 6254 4709 Size: 32 1481 32 5095 Fragments arranged by size: 5095 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2574 2658 2574 Size: 84 6556 PshAI GACnn'nnGTC Cuts at: 1613 2572 1613 Size: 959 5681 PsiI TTA'TAA Cuts at: 4210 4210 Size: 6640 PstI C_TGCA'G Cuts at: 830 2386 3651 3992 830 Size: 1556 1265 341 3478 Fragments arranged by size: 3478 1556 1265 341 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6640 PvuII CAG'CTG Cuts at: 2032 2441 3179 2032 Size: 409 738 5493 Fragments arranged by size: 5493 738 409 SacI G_AGCT'C Cuts at: 3937 3937 Size: 6640 SalI G'TCGA_C Cuts at: 1936 3982 1936 Size: 2046 4594 SapI GCTCTTCn'nnn_ Cuts at: 2514 2514 Size: 6640 SbfI CC_TGCA'GG Cuts at: 2386 3992 2386 Size: 1606 5034 ScaI AGT'ACT Cuts at: 4888 6600 4888 Size: 1712 4928 SfcI C'TryA_G Cuts at: 826 1045 2092 2382 3647 3988 5123 5804 5995 Size: 219 1047 290 1265 341 1135 681 191 Cuts at: 5995 826 Size: 1471 Fragments arranged by size: 1471 1265 1135 1047 681 341 290 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6640 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6640 SmaI CCC'GGG Cuts at: 1872 3967 1872 Size: 2095 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4744 5645 5907 820 Size: 197 926 2801 901 262 1553 Fragments arranged by size: 2801 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4431 493 Size: 3938 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6640 SphI G_CATG'C Cuts at: 1132 3351 3998 1132 Size: 2219 647 3774 Fragments arranged by size: 3774 2219 647 SspI AAT'ATT Cuts at: 5 52 4564 5 Size: 47 4512 2081 Fragments arranged by size: 4512 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6640 StyI C'CwwG_G Cuts at: 513 1065 3094 3471 4079 513 Size: 552 2029 377 608 3074 Fragments arranged by size: 3074 2029 608 552 377 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2084 3433 3525 4667 4684 4837 5022 Size: 1255 504 1349 92 1142 17 153 185 Cuts at: 5022 5467 325 Size: 445 1498 Fragments arranged by size: 1498 1349 1255 1142 504 445 185 153 92 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2007 2034 3549 Size: 274 80 33 51 1443 30 27 1515 Cuts at: 3549 4886 6598 96 Size: 1337 1712 138 Fragments arranged by size: 1712 1515 1443 1337 274 138 80 51 33 30 27 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3772 3804 6600 6632 3772 Size: 32 2796 32 3780 Fragments arranged by size: 3780 2796 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6640 XbaI T'CTAG_A Cuts at: 3391 3976 3391 Size: 585 6055 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6640 XmaI C'CCGG_G Cuts at: 1870 3965 1870 Size: 2095 4545 XmnI GAAnn'nnTTC Cuts at: 4769 4769 Size: 6640 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4448 276 Size: 53 83 186 936 2914 2468 Fragments arranged by size: 2914 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI BsiWI BstZ17I FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI XcmI