################################## # NO CUT ENZYMES ( 145 enzymes) # ################################## AatII Acc65I AccIII AclI AcyI AflII AflIII AgeI AhdI AlwNI AocI Aor51HI AscI AseI AsnI Asp718I AspEI AviII AvrII BamHI BbeI BbrPI BbsI BbvCI BciVI BclI BfaI BfrI BglI BglII BlnI BlpI Bpu1102I BpuAI BsaAI BsaBI BsaHI BseAI BsgI BsiEI BsiWI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstXI BstZ17I Bsu36I CelII CfoI ClaI CpoI DraI DrdI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRV EcoT22I FbaI FseI FspI HaeII HhaI Hin1I HinP1I HindIII HpaI KasI KpnI Ksp632I KspI MaeI MamI MboII MfeI MluI MroI MseI MunI NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PstI PvuI RcaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfcI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI Tru9I XbaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 40 enzymes) # ################################### AccI 364 GTCTAC GT^[AC][GT]AC AccII 1287 CGCG CG^CG AlwI 737 GGATC GGATC(4/5) Asp700I 899 GAAACCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspI 274 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC AvaI 957 CTCGGG C^[CT]CG[GA]G BalI 2 TGGCCA TGG^CCA BbvI 359 GCTGC GCAGC(8/12) BsaWI 684 ACCGGA [AT]^CCGG[AT] BsmBI 677 GAGACG CGTCTC(1/5) BsoBI 957 CTCGGG C^[CT]CG[GA]G BsrFI 863 ACCGGC [GA]^CCGG[CT] BstUI 1287 CGCG CG^CG BstYI 737 GGATCT [GA]^GATC[CT] Cfr10I 863 ACCGGC [GA]^CCGG[CT] DraIII 508 CACATAGTG CAC[ACGT][ACGT][ACGT]^GTG DsaI 828 CCATGG C^C[GA][CT]GG EaeI 2 TGGCCA [CT]^GGCC[GA] EcoRI 477 GAATTC G^AATTC HgaI 362 GCGTC GACGC(5/10) HincII 310 GTCAAC GT[CT]^[GA]AC HindII 310 GTCAAC GT[CT]^[GA]AC MflI 737 GGATCT [GA]^GATC[CT] MluNI 2 TGGCCA TGG^CCA MscI 2 TGGCCA TGG^CCA MvnI 1287 CGCG CG^CG NcoI 828 CCATGG C^CATGG PflFI 274 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC PflMI 531 CCATCGAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PleI 379 GACTC GAGTC(4/5) PpuMI 960 GGGACCT [GA]G^G[AT]CC[CT] SmlI 933 CTCAAG C^T[CT][GA]AG TaqI 534 TCGA T^CGA TfiI 158 GATTC G^A[AT]TC TseI 359 GCTGC G^C[AT]GC Tth111I 274 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC TthHB8I 534 TCGA T^CGA Van91I 531 CCATCGAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XhoII 737 GGATCT [GA]^GATC[CT] XmnI 899 GAAACCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 24 enzymes) # ################################### AcsI 477 GAATTC [GA]^AATT[CT] 918 GAATTT Alw44I 762 GTGCAC G^TGCAC 1117 GTGCAC ApaI 395 GGGCCC GGGCC^C 1045 GGGCCC ApaLI 762 GTGCAC G^TGCAC 1117 GTGCAC ApoI 477 GAATTC [GA]^AATT[CT] 918 GAATTT BcgI 1195 GCACCCACTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 1267 GCAGTGGCCTCG BcnI 375 CCCGG CC^[GC]GG 1110 CCCGG BpmI 348 CTCCAG CTGGAG(16/14) 133 CTGGAG BsaI 345 GGTCTC GGTCTC(1/5) 850 GGTCTC BsmFI 960 GGGAC GGGAC(10/14) 280 GTCCC BsrBI 794 CCGCTC CCGCTC(-3/-3) 1307 GAGCGG BsrDI 1245 CATTGC GCAATG(2/0) 1079 GCAATG DpnII 126 GATC ^GATC 738 GATC EcoT14I 656 CCAAGG C^C[AT][AT]GG 828 CCATGG Fnu4HI 359 GCTGC GC^[ACGT]GC 793 GCCGC HinfI 158 GATTC G^A[ACGT]TC 379 GACTC ItaI 359 GCTGC GC^[ACGT]GC 793 GCCGC MaeII 308 ACGT A^CGT 680 ACGT MboI 126 GATC ^GATC 738 GATC NciI 375 CCCGG CC^[GC]GG 1110 CCCGG NdeII 126 GATC ^GATC 738 GATC NspI 708 GCATGT [GA]CATG^[CT] 890 ACATGC Sau3AI 126 GATC ^GATC 738 GATC StyI 656 CCAAGG C^C[AT][AT]GG 828 CCATGG ######################################### # 3 OR MORE CUT ENZYMES ( 49 enzymes) # ######################################### AciI AfaI AluI AspHI AvaII BanI BanII BmyI BsaJI BseRI BsiHKAI BsiYI BslI BsmAI Bsp1286I BsrI BssKI BstF5I BstNI Cac8I Cfr13I DdeI DraII EcoO109I EcoRII FauI FokI HaeIII HapII HpaII HphI MaeIII MnlI MslI MspA1I MspI MvaI MwoI NlaIII NlaIV PvuII RsaI Sau96I ScrFI SfaNI Tsp45I Tsp509I TspRI XcmI