Restriction map for
pFN21AB7761
(Circular) (Six-base) MAPSORT of: pFN21AB7761.seq Check: 2831 from: 1 to: 6049
pFN21AB7761 6049 bp
With 182 enzymes: SgfI *
December 28, 2011 15:26 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3859 278
Size: 53 83 186 936 2323 2468
Fragments arranged by size:
2468 2323 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2370 3392 1937
Size: 433 1022 4594
Fragments arranged by size:
4594 1022 433
Acc65I G'GTAC_C
Cuts at: 3370 3370
Size: 6049
AclI AA'CG_TT
Cuts at: 1563 4176 4549 1563
Size: 2613 373 3063
Fragments arranged by size:
3063 2613 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2069 2111 2413 4112 5496 5764 1632
Size: 437 42 302 1699 1384 268 1917
Fragments arranged by size:
1917 1699 1384 437 302 268 42
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5852
AflIII A'CryG_T
Cuts at: 1203 4948 5925 1203
Size: 3745 977 1327
Fragments arranged by size:
3745 1327 977
AgeI A'CCGG_T
Cuts at: 4880 4880
Size: 6049
AhdI GACnn_n'nnGTC
Cuts at: 4778 4778
Size: 6049
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6049
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2966 1184
Size: 1782 4267
AlwNI CAG_nnn'CTG
Cuts at: 3792 5364 3792
Size: 1572 4477
ApaI G_GGCC'C
Cuts at: 2404 3054 2404
Size: 650 5399
ApaLI G'TGCA_C
Cuts at: 2767 3122 4106 5262 2767
Size: 355 984 1156 3554
Fragments arranged by size:
3554 1156 984 355
ApoI r'AATT_y
Cuts at: 1493 2482 2923 3358 3584 3736 5845 1493
Size: 989 441 435 226 152 2109 1697
Fragments arranged by size:
2109 1697 989 441 435 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4603 160
Size: 872 3571 1606
Fragments arranged by size:
3571 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6049
AvaI C'yCGr_G
Cuts at: 1870 1943 2962 3374 1870
Size: 73 1019 412 4545
Fragments arranged by size:
4545 1019 412 73
BamHI G'GATC_C
Cuts at: 3379 3746 3379
Size: 367 5682
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2329 3089 3321 3370 4825
Size: 325 217 627 542 760 232 49 1455
Cuts at: 4825 618
Size: 1842
Fragments arranged by size:
1842 1455 760 627 542 325 232 217
49
BanII G_rGCy'C
Cuts at: 1391 2404 3054 3237 3368 1391
Size: 1013 650 183 131 4072
Fragments arranged by size:
4072 1013 650 183 131
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5078
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3189 3223 3261 3295 4240 4274 1903
Size: 34 1252 34 38 34 945 34 3678
Fragments arranged by size:
3678 1252 945 38 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3941 5151 3941
Size: 1210 4839
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6049
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4660 136
Size: 107 122 71 631 605 2988 1525
Fragments arranged by size:
2988 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6044 6044
Size: 6049
BlpI GC'TnA_GC
Cuts at: 3466 3466
Size: 6049
Bme1580I G_kGCm'C
Cuts at: 1790 2027 2334 2404 2536 2771 3054 3126 3324
Size: 237 307 70 132 235 283 72 198
Cuts at: 3324 4110 5266 1790
Size: 786 1156 2573
Fragments arranged by size:
2573 1156 786 307 283 237 235 198
132 72 70
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6049
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2566 3096 3780
Size: 416 53 83 399 24 1149 530 684
Cuts at: 3780 4738 442
Size: 958 1753
Fragments arranged by size:
1753 1149 958 684 530 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6049
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2716 3164 2716
Size: 448 5601
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2159 2338 4709 1239
Size: 920 179 2371 2579
Fragments arranged by size:
2579 2371 920 179
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2923 4174 5039 5337 2923
Size: 1251 865 298 3635
Fragments arranged by size:
3635 1251 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2356 2861 4712 882
Size: 617 857 505 1851 2219
Fragments arranged by size:
2219 1851 857 617 505
BsaAI yAC'GTr
Cuts at: 493 1204 3840 493
Size: 711 2636 2702
Fragments arranged by size:
2702 2636 711
BsaBI GATnn'nnATC
Cuts at: 6043 6043
Size: 6049
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3856 4238 4873 275
Size: 53 83 186 936 2323 382 635 1451
Fragments arranged by size:
2323 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2689 4482 4880 5154 5301 5712 2689
Size: 1793 398 274 147 411 3026
Fragments arranged by size:
3026 1793 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2369 2399 1178
Size: 30 165 30 966 30 4828
Fragments arranged by size:
4828 966 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2618 2793 3140 1186
Size: 1432 175 347 4095
Fragments arranged by size:
4095 1432 347 175
BseYI C'CCAG_C
Cuts at: 2020 2036 3053 3160 3297 5252 5748 2020
Size: 16 1017 107 137 1955 496 2321
Fragments arranged by size:
2321 1955 1017 496 137 107 16
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5476
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3499 4260 5288 1540
Size: 275 186 1498 761 1028 2301
Fragments arranged by size:
2301 1498 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2771 2847 3126 3189 4110 4195 5266 2771
Size: 76 279 63 921 85 1071 3554
Fragments arranged by size:
3554 1071 921 279 85 76 63
BsmI GAATG_Cn'
Cuts at: 3560 3653 3560
Size: 93 5956
BsmBI CGTCTCn'nnnn_
Cuts at: 2676 2676
Size: 6049
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2027 2334 2404 2536 2771 2847 3054
Size: 399 237 307 70 132 235 76 207
Cuts at: 3054 3126 3189 3237 3324 3368 4110 4195 5266
Size: 72 63 48 87 44 742 85 1071
Cuts at: 5266 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 399 307 237 235 207
132 87 85 76 72 70 63 48
44
BspEI T'CCGG_A
Cuts at: 5712 5712
Size: 6049
BspHI T'CATG_A
Cuts at: 3938 5668 3938
Size: 1730 4319
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3404 844
Size: 2560 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2801 3314 3936 1394
Size: 429 978 513 622 3507
Fragments arranged by size:
3507 978 622 513 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3091 3249 4544 4718 65
Size: 1183 1843 158 1295 174 1396
Fragments arranged by size:
1843 1396 1295 1183 174 158
BsrFI r'CCGG_y
Cuts at: 1700 1810 2868 4693 4880 1700
Size: 110 1058 1825 187 2869
Fragments arranged by size:
2869 1825 1058 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6049
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6049
BssSI C'ACGA_G
Cuts at: 4109 5121 4109
Size: 1012 5037
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6049
BstBI TT'CG_AA
Cuts at: 4853 4853
Size: 6049
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6049
BstYI r'GATC_y
Cuts at: 1972 2742 3379 3746 4131 4148 5600 5686 5698
Size: 770 637 367 385 17 1452 86 12
Cuts at: 5698 6044 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 770 637 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2833 513
Size: 552 939 829 3729
Fragments arranged by size:
3729 939 829 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2055 487
Size: 683 56 829 4481
Fragments arranged by size:
4481 829 683 56
BtsI GCAGTG_nn'
Cuts at: 3279 3565 4359 4379 3279
Size: 286 794 20 4949
Fragments arranged by size:
4949 794 286 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6049
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6014
DraI TTT'AAA
Cuts at: 3353 3353
Size: 6049
DraIII CAC_nnn'GTG
Cuts at: 2518 2518
Size: 6049
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5056 809
Size: 507 3740 1802
Fragments arranged by size:
3740 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2007 3496 4385
Size: 54 1425 50 275 18 177 1489 889
Cuts at: 4385 6014 8
Size: 1629 43
Fragments arranged by size:
1629 1489 1425 889 275 177 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3496 1537
Size: 275 1684 4090
Fragments arranged by size:
4090 1684 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3979 1353
Size: 2626 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4636 5010 5156 387
Size: 1064 138 3047 374 146 1280
Fragments arranged by size:
3047 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2069 2111 2159 2338 2413 4112 4709
Size: 393 437 42 48 179 75 1699 597
Cuts at: 4709 5496 5764 1239
Size: 787 268 1524
Fragments arranged by size:
1699 1524 787 597 437 393 268 179
75 48 42
EcoO109I rG'GnC_Cy
Cuts at: 2400 2966 3050 3098 2400
Size: 566 84 48 5351
Fragments arranged by size:
5351 566 84 48
EcoRI G'AATT_C
Cuts at: 2482 3358 3736 2482
Size: 876 378 4795
Fragments arranged by size:
4795 876 378
FspI TGC'GCA
Cuts at: 4555 4555
Size: 6049
HaeII r_GCGC'y
Cuts at: 1678 5196 1678
Size: 3518 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2401 2433 2578 2599 2610
Size: 32 135 32 829 32 145 21 11
Cuts at: 2610 2631 2710 2727 2742 2759 4690 4722 4764
Size: 21 79 17 15 17 1931 32 42
Cuts at: 4764 4796 1373
Size: 32 2626
Fragments arranged by size:
2626 1931 829 145 135 79 42 32
32 32 32 32 21 21 17 17
15 11
HincII GTy'rAC
Cuts at: 669 1657 1938 2317 3393 3639 669
Size: 988 281 379 1076 246 3079
Fragments arranged by size:
3079 1076 988 379 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6049
HpaI GTT'AAC
Cuts at: 3639 3639
Size: 6049
KpnI G_GTAC'C
Cuts at: 3374 3374
Size: 6049
MfeI C'AATT_G
Cuts at: 3648 3648
Size: 6049
MluI A'CGCG_T
Cuts at: 5925 5925
Size: 6049
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2226 2506 5163 5347 1305
Size: 921 280 2657 184 2007
Fragments arranged by size:
2657 2007 921 280 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2009 10
Size: 54 1425 343 177 4050
Fragments arranged by size:
4050 1425 343 177 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2009 2496 2582 2838 3254 4007 4366
Size: 648 843 487 86 256 416 753 359
Cuts at: 4366 4525 518
Size: 159 2042
Fragments arranged by size:
2042 843 753 648 487 416 359 256
159 86
MspA1I CmG'CkG
Cuts at: 1606 2304 2336 2364 2976 3164 3465 4142 5290
Size: 698 32 28 612 188 301 677 1148
Cuts at: 5290 5535 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 698 677 612 301 245 188
32 28
NcoI C'CATG_G
Cuts at: 513 1065 2004 2833 513
Size: 552 939 829 3729
Fragments arranged by size:
3729 939 829 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6049
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6049
NotI GC'GGCC_GC
Cuts at: 3496 3496
Size: 6049
NspI r_CATG'y
Cuts at: 1132 1229 2717 2899 3407 4952 1132
Size: 97 1488 182 508 1545 2229
Fragments arranged by size:
2229 1545 1488 508 182 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6049
PciI A'CATG_T
Cuts at: 4948 4948
Size: 6049
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2542 1108
Size: 1434 4615
PfoI T'CCnGG_A
Cuts at: 2136 2136
Size: 6049
PmeI GTTT'AAAC
Cuts at: 3353 3353
Size: 6049
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4118 4150 5631 5663 4118
Size: 32 1481 32 4504
Fragments arranged by size:
4504 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2966 2966
Size: 6049
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6049
PsiI TTA'TAA
Cuts at: 3619 3619
Size: 6049
PspOMI G'GGCC_C
Cuts at: 2400 3050 2400
Size: 650 5399
PstI C_TGCA'G
Cuts at: 830 3401 830
Size: 2571 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6049
PvuII CAG'CTG
Cuts at: 2364 2976 3164 2364
Size: 612 188 5249
Fragments arranged by size:
5249 612 188
SalI G'TCGA_C
Cuts at: 1936 3391 1936
Size: 1455 4594
SbfI CC_TGCA'GG
Cuts at: 3401 3401
Size: 6049
ScaI AGT'ACT
Cuts at: 4297 6009 4297
Size: 1712 4337
SfcI C'TryA_G
Cuts at: 826 1045 3397 4532 5213 5404 826
Size: 219 2352 1135 681 191 1471
Fragments arranged by size:
2352 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6049
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6049
SmaI CCC'GGG
Cuts at: 1872 3376 1872
Size: 1504 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2938 4153 5054 5316 820
Size: 197 926 995 1215 901 262 1553
Fragments arranged by size:
1553 1215 995 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3840 493
Size: 3347 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6049
SphI G_CATG'C
Cuts at: 1132 3407 1132
Size: 2275 3774
SspI AAT'ATT
Cuts at: 5 52 3973 5
Size: 47 3921 2081
Fragments arranged by size:
3921 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6049
StyI C'CwwG_G
Cuts at: 513 1065 2004 2661 2833 3488 513
Size: 552 939 657 172 655 3074
Fragments arranged by size:
3074 939 657 655 552 172
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2317 2843 3209 3217 4076 4093 4246
Size: 1255 737 526 366 8 859 17 153
Cuts at: 4246 4431 4876 325
Size: 185 445 1498
Fragments arranged by size:
1498 1255 859 737 526 445 366 185
153 17 8
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2262 2628 4295
Size: 274 80 33 51 1443 285 366 1667
Cuts at: 4295 6007 96
Size: 1712 138
Fragments arranged by size:
1712 1667 1443 366 285 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2369 2401 6009 6041 2369
Size: 32 3608 32 2377
Fragments arranged by size:
3608 2377 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2282 1316
Size: 966 5083
XbaI T'CTAG_A
Cuts at: 3385 3385
Size: 6049
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2017 2650 2831 2017
Size: 633 181 5235
Fragments arranged by size:
5235 633 181
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6049
XmaI C'CCGG_G
Cuts at: 1870 3374 1870
Size: 1504 4545
XmnI GAAnn'nnTTC
Cuts at: 2908 4178 2908
Size: 1270 4779
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3857 276
Size: 53 83 186 936 2323 2468
Fragments arranged by size:
2468 2323 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI
PspOMI PstI PvuI PvuII SalI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI
BfrBI Bpu10I BsiWI BstXI BstZ17I Bsu36I EcoICRI EcoNI
EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PacI PmlI PspXI PsrI RsrII SacI
SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI