################################## # NO CUT ENZYMES ( 138 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcyI AflII AgeI AhdI Alw44I AlwNI AocI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AviII AvrII BamHI BanI BbeI BbrPI BbsI BbvCI BcgI BcnI BfrI BglI BlnI BlpI Bpu1102I BpuAI BsaAI BsaHI BsaI BsaWI BseAI BsiEI BsiHKAI BsiWI BsmAI BsmBI Bsp1407I BspDI BspEI BspHI BspMI BsrDI BsrFI BsrGI BssHII Bst1107I BstAPI BstEII BstPI BstZ17I Bsu36I CelII Cfr10I ClaI CpoI DrdI EagI Eam1105I EclXI Eco52I Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT22I FseI FspI HgaI Hin1I HindIII HpaI KasI KpnI KspI MfeI MluI MroI MslI MspA1I MunI NaeI NarI NciI NgoMIV NheI NotI NruI NsiI PacI PflFI PflMI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsrII SacI SacII SalI SbfI ScaI SexAI SfiI SfoI SgrAI SmaI SnaBI SpeI Sse8387I SspBI SspI SwaI Tth111I Van91I XbaI XcmI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 44 enzymes) # ################################### AccII 91 CGCG CG^CG AflIII 1259 ACATGT A^C[GA][CT]GT Aor51HI 395 AGCGCT AGC^GCT ApaI 208 GGGCCC GGGCC^C AvaI 173 CTCGAG C^[CT]CG[GA]G BciVI 1276 GGATAC GTATCC(6/5) BclI 318 TGATCA T^GATCA BglII 714 AGATCT A^GATCT BpmI 287 CTCCAG CTGGAG(16/14) BsaBI 1277 GATACAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsgI 43 GTGCAG GTGCAG(16/14) BsmFI 1263 GTCCC GGGAC(10/14) BsmI 24 GAATGC GAATGC(1/-1) BsoBI 173 CTCGAG C^[CT]CG[GA]G BspLU11I 1259 ACATGT A^CATGT BsrBI 188 CCGCTC CCGCTC(-3/-3) BssSI 491 CACGAG CACGAG(-5/-1) BstBI 797 TTCGAA TT^CGAA BstUI 91 CGCG CG^CG DraI 309 TTTAAA TTT^AAA DraIII 840 CACAAGGTG CAC[ACGT][ACGT][ACGT]^GTG DsaI 366 CCATGG C^C[GA][CT]GG Eco47III 395 AGCGCT AGC^GCT FauI 1181 CCCGC CCCGC(4/6) FbaI 318 TGATCA T^GATCA HapII 21 CCGG C^CGG HincII 667 GTCAAC GT[CT]^[GA]AC HindII 667 GTCAAC GT[CT]^[GA]AC HpaII 21 CCGG C^CGG MaeII 561 ACGT A^CGT MamI 1277 GATACAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MspI 21 CCGG C^CGG MvnI 91 CGCG CG^CG NcoI 366 CCATGG C^CATGG NdeI 685 CATATG CA^TATG NspV 797 TTCGAA TT^CGAA PaeR7I 173 CTCGAG C^TCGAG PleI 665 GAGTC GAGTC(4/5) SapI 1079 GCTCTTC GCTCTTC(1/4) SfcI 731 CTGTAG C^T[GA][CT]AG SfuI 797 TTCGAA TT^CGAA SphI 257 GCATGC GCATG^C StuI 75 AGGCCT AGG^CCT XhoI 173 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 21 enzymes) # ################################### AciI 188 CCGC CCGC(-3/-1) 1182 CCGC AlwI 54 GATCC GGATC(4/5) 752 GGATC AvaII 201 GGACC G^G[AT]CC 738 GGACC BanII 208 GGGCCC G[GA]GC[CT]^C 778 GAGCCC BbvI 1157 GCAGC GCAGC(8/12) 517 GCTGC BmyI 208 GGGCCC G[AGT]GC[ACT]^C 778 GAGCCC Bsp1286I 208 GGGCCC G[AGT]GC[ACT]^C 778 GAGCCC BstXI 619 CCAGGGAACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1266 CCAAAAAGATGG CfoI 130 GCGC GCG^C 396 GCGC EcoT14I 330 CCTTGG C^C[AT][AT]GG 366 CCATGG HaeII 129 GGCGCT [GA]GCGC^[CT] 395 AGCGCT HhaI 130 GCGC GCG^C 396 GCGC HinP1I 130 GCGC G^CGC 396 GCGC MseI 310 TTAA T^TAA 425 TTAA NspI 257 GCATGC [GA]CATG^[CT] 1259 ACATGT PpuMI 200 AGGACCC [GA]G^G[AT]CC[CT] 737 AGGACCT SmlI 173 CTCGAG C^T[CT][GA]AG 772 CTCAAG StyI 330 CCTTGG C^C[AT][AT]GG 366 CCATGG Tru9I 310 TTAA T^TAA 425 TTAA TseI 517 GCTGC G^C[AT]GC 1157 GCAGC TspRI 242 CACTG CA[GC]TG[ACGT][ACGT]^ 729 CACTG ######################################### # 3 OR MORE CUT ENZYMES ( 55 enzymes) # ######################################### AcsI AfaI AluI ApoI BalI BfaI BsaJI BseRI BsiYI BslI BsrI BssKI BstF5I BstNI BstYI Cac8I Cfr13I DdeI DpnII DraII EaeI EarI EcoO109I EcoRII Fnu4HI FokI HaeIII HinfI HphI ItaI Ksp632I MaeI MaeIII MboI MboII MflI MluNI MnlI MscI MvaI MwoI NdeII NlaIII NlaIV RsaI Sau3AI Sau96I ScrFI SfaNI TaqI TfiI Tsp45I Tsp509I TthHB8I XhoII