(Circular) (Six-base) MAPSORT of: pFN21AE1904.seq Check: 5813 from: 1 to: 6007 pFN21AE1904 6007 bp With 182 enzymes: SgfI * August 16, 2010 14:52 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3817 278 Size: 53 83 186 936 2281 2468 Fragments arranged by size: 2468 2281 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3350 1937 Size: 1413 4594 Acc65I G'GTAC_C Cuts at: 3328 3328 Size: 6007 AclI AA'CG_TT Cuts at: 1563 4134 4507 1563 Size: 2571 373 3063 Fragments arranged by size: 3063 2571 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4070 5454 5722 1632 Size: 2438 1384 268 1917 Fragments arranged by size: 2438 1917 1384 268 AfeI AGC'GCT Cuts at: 2402 2402 Size: 6007 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5810 AflIII A'CryG_T Cuts at: 1203 3264 4906 5883 1203 Size: 2061 1642 977 1327 Fragments arranged by size: 2061 1642 1327 977 AgeI A'CCGG_T Cuts at: 4838 4838 Size: 6007 AhdI GACnn_n'nnGTC Cuts at: 4736 4736 Size: 6007 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6007 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6007 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2388 2420 2388 Size: 32 5975 AlwNI CAG_nnn'CTG Cuts at: 3750 5322 3750 Size: 1572 4435 ApaI G_GGCC'C Cuts at: 2217 2217 Size: 6007 ApaLI G'TGCA_C Cuts at: 4064 5220 4064 Size: 1156 4851 ApoI r'AATT_y Cuts at: 1493 2490 2557 2771 3144 3230 3316 3542 3694 Size: 997 67 214 373 86 86 226 152 Cuts at: 3694 5803 1493 Size: 2109 1697 Fragments arranged by size: 2109 1697 997 373 226 214 152 86 86 67 AseI AT'TA_AT Cuts at: 160 1032 4561 160 Size: 872 3529 1606 Fragments arranged by size: 3529 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6007 AvaI C'yCGr_G Cuts at: 1870 1943 2178 3332 1870 Size: 73 235 1154 4545 Fragments arranged by size: 4545 1154 235 73 BamHI G'GATC_C Cuts at: 3337 3704 3337 Size: 367 5640 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3328 4783 618 Size: 325 217 627 1541 1455 1842 Fragments arranged by size: 1842 1541 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2217 2787 3326 1391 Size: 826 570 539 4072 Fragments arranged by size: 4072 826 570 539 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5036 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 4198 4232 1903 Size: 34 50 34 2177 34 3678 Fragments arranged by size: 3678 2177 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3275 3899 5109 3275 Size: 624 1210 4173 Fragments arranged by size: 4173 1210 624 BclI T'GATC_A Cuts at: 1548 2323 1548 Size: 775 5232 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4618 136 Size: 107 122 71 631 605 2946 1525 Fragments arranged by size: 2946 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2719 6002 2719 Size: 3283 2724 BlpI GC'TnA_GC Cuts at: 3424 3424 Size: 6007 Bme1580I G_kGCm'C Cuts at: 1790 2217 4068 5224 1790 Size: 427 1851 1156 2573 Fragments arranged by size: 2573 1851 1156 427 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6007 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2140 3738 4696 Size: 416 53 83 399 24 723 1598 958 Cuts at: 4696 442 Size: 1753 Fragments arranged by size: 1753 1598 958 723 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6007 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2211 2211 Size: 6007 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2277 4667 1239 Size: 1038 2390 2579 Fragments arranged by size: 2579 2390 1038 Bpu10I CC'TnA_GC Cuts at: 2196 3050 2196 Size: 854 5153 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2762 4132 4997 5295 2762 Size: 1370 865 298 3474 Fragments arranged by size: 3474 1370 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4670 882 Size: 617 3171 2219 Fragments arranged by size: 3171 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3798 493 Size: 711 2594 2702 Fragments arranged by size: 2702 2594 711 BsaBI GATnn'nnATC Cuts at: 3286 6001 3286 Size: 2715 3292 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3814 4196 4831 275 Size: 53 83 186 936 2281 382 635 1451 Fragments arranged by size: 2281 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4440 4838 5112 5259 5670 4440 Size: 398 274 147 411 4777 Fragments arranged by size: 4777 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3218 3248 1178 Size: 30 165 30 1815 30 3937 Fragments arranged by size: 3937 1815 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2113 2266 2376 1186 Size: 927 153 110 4817 Fragments arranged by size: 4817 927 153 110 BseYI C'CCAG_C Cuts at: 5210 5706 5210 Size: 496 5511 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2069 1163 Size: 573 333 5101 Fragments arranged by size: 5101 573 333 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3457 4218 5246 1540 Size: 275 186 1456 761 1028 2301 Fragments arranged by size: 2301 1456 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4068 4153 5224 4068 Size: 85 1071 4851 Fragments arranged by size: 4851 1071 85 BsmI GAATG_Cn' Cuts at: 2035 3518 3611 2035 Size: 1483 93 4431 Fragments arranged by size: 4431 1483 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2217 2787 3326 4068 4153 5224 1391 Size: 399 427 570 539 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 570 539 427 399 85 BspEI T'CCGG_A Cuts at: 5670 5670 Size: 6007 BspHI T'CATG_A Cuts at: 3896 5626 3896 Size: 1730 4277 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3362 844 Size: 2518 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2195 3894 1394 Size: 429 372 1699 3507 Fragments arranged by size: 3507 1699 429 372 BsrDI GCAATG_nn' Cuts at: 65 1248 4502 4676 65 Size: 1183 3254 174 1396 Fragments arranged by size: 3254 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4651 4838 1700 Size: 110 2841 187 2869 Fragments arranged by size: 2869 2841 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6007 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6007 BssSI C'ACGA_G Cuts at: 2496 4067 5079 2496 Size: 1571 1012 3424 Fragments arranged by size: 3424 1571 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6007 BstBI TT'CG_AA Cuts at: 2803 4811 2803 Size: 2008 3999 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6007 BstXI CCAn_nnnn'nTGG Cuts at: 2631 3278 2631 Size: 647 5360 BstYI r'GATC_y Cuts at: 1972 2058 2719 2757 3337 3704 4089 4106 5558 Size: 86 661 38 580 367 385 17 1452 Cuts at: 5558 5644 5656 6002 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 661 580 385 367 346 86 86 38 17 12 BtgI C'CryG_G Cuts at: 513 1065 2371 513 Size: 552 1306 4149 Fragments arranged by size: 4149 1306 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5268 Fragments arranged by size: 5268 683 56 BtsI GCAGTG_nn' Cuts at: 3523 4317 4337 3523 Size: 794 20 5193 Fragments arranged by size: 5193 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6007 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2926 2961 529 Size: 35 2362 35 3575 Fragments arranged by size: 3575 2362 35 35 DraI TTT'AAA Cuts at: 2316 3311 2316 Size: 995 5012 DraIII CAC_nnn'GTG Cuts at: 2850 2850 Size: 6007 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5014 809 Size: 507 3698 1802 Fragments arranged by size: 3698 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2633 2820 2934 Size: 54 1425 50 275 18 803 187 114 Cuts at: 2934 3151 3454 4343 5972 8 Size: 217 303 889 1629 43 Fragments arranged by size: 1629 1425 889 803 303 275 217 187 114 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3454 1537 Size: 275 1642 4090 Fragments arranged by size: 4090 1642 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2885 3091 3169 3301 3937 1353 Size: 1532 206 78 132 636 3423 Fragments arranged by size: 3423 1532 636 206 132 78 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4594 4968 5114 387 Size: 1064 138 3005 374 146 1280 Fragments arranged by size: 3005 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2277 4070 4667 5454 5722 1239 Size: 393 645 1793 597 787 268 1524 Fragments arranged by size: 1793 1524 787 645 597 393 268 EcoO109I rG'GnC_Cy Cuts at: 2206 2214 2743 2206 Size: 8 529 5470 Fragments arranged by size: 5470 529 8 EcoRI G'AATT_C Cuts at: 3316 3694 3316 Size: 378 5629 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2351 2351 Size: 6007 FspI TGC'GCA Cuts at: 4513 4513 Size: 6007 HaeII r_GCGC'y Cuts at: 1678 2138 2404 5154 1678 Size: 460 266 2750 2531 Fragments arranged by size: 2750 2531 460 266 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3116 3148 4648 4680 4722 Size: 32 135 32 1544 32 1500 32 42 Cuts at: 4722 4754 1373 Size: 32 2626 Fragments arranged by size: 2626 1544 1500 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2674 3351 3597 669 Size: 988 281 736 677 246 3079 Fragments arranged by size: 3079 988 736 677 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6007 HpaI GTT'AAC Cuts at: 3597 3597 Size: 6007 KpnI G_GTAC'C Cuts at: 3332 3332 Size: 6007 MfeI C'AATT_G Cuts at: 3606 3606 Size: 6007 MluI A'CGCG_T Cuts at: 5883 5883 Size: 6007 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3261 5121 5305 1305 Size: 1956 1860 184 2007 Fragments arranged by size: 2007 1956 1860 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2635 2822 2936 3153 10 Size: 54 1425 343 803 187 114 217 2864 Fragments arranged by size: 2864 1425 803 343 217 187 114 54 MslI CAynn'nnrTG Cuts at: 518 1166 3965 4324 4483 518 Size: 648 2799 359 159 2042 Fragments arranged by size: 2799 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3423 4100 5248 5493 1606 Size: 1817 677 1148 245 2120 Fragments arranged by size: 2120 1817 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2371 513 Size: 552 1306 4149 Fragments arranged by size: 4149 1306 552 NdeI CA'TA_TG Cuts at: 387 2691 387 Size: 2304 3703 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6007 NotI GC'GGCC_GC Cuts at: 3454 3454 Size: 6007 NspI r_CATG'y Cuts at: 1132 1229 2266 3268 3365 4910 1132 Size: 97 1037 1002 97 1545 2229 Fragments arranged by size: 2229 1545 1037 1002 97 97 PasI CC'CwG_GG Cuts at: 1348 2210 2624 1348 Size: 862 414 4731 Fragments arranged by size: 4731 862 414 PciI A'CATG_T Cuts at: 3264 4906 3264 Size: 1642 4365 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6007 PmeI GTTT'AAAC Cuts at: 3311 3311 Size: 6007 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4076 4108 5589 5621 4076 Size: 32 1481 32 4462 Fragments arranged by size: 4462 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2206 2743 2206 Size: 537 5470 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6007 PsiI TTA'TAA Cuts at: 2477 3577 2477 Size: 1100 4907 PspOMI G'GGCC_C Cuts at: 2213 2213 Size: 6007 PstI C_TGCA'G Cuts at: 830 3359 830 Size: 2529 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6007 SalI G'TCGA_C Cuts at: 1936 3349 1936 Size: 1413 4594 SapI GCTCTTCn'nnn_ Cuts at: 3091 3091 Size: 6007 SbfI CC_TGCA'GG Cuts at: 3359 3359 Size: 6007 ScaI AGT'ACT Cuts at: 4255 5967 4255 Size: 1712 4295 SfcI C'TryA_G Cuts at: 826 1045 2736 3355 4490 5171 5362 826 Size: 219 1691 619 1135 681 191 1471 Fragments arranged by size: 1691 1471 1135 681 619 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6007 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6007 SmaI CCC'GGG Cuts at: 1872 3334 1872 Size: 1462 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2178 2777 4111 5012 5274 820 Size: 197 926 235 599 1334 901 262 1553 Fragments arranged by size: 1553 1334 926 901 599 262 235 197 SnaBI TAC'GTA Cuts at: 493 3798 493 Size: 3305 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6007 SphI G_CATG'C Cuts at: 1132 2266 3365 1132 Size: 1134 1099 3774 Fragments arranged by size: 3774 1134 1099 SspI AAT'ATT Cuts at: 5 52 3931 5 Size: 47 3879 2081 Fragments arranged by size: 3879 2081 47 StuI AGG'CCT Cuts at: 1517 2082 1517 Size: 565 5442 StyI C'CwwG_G Cuts at: 513 1065 2335 2371 3446 513 Size: 552 1270 36 1075 3074 Fragments arranged by size: 3074 1270 1075 552 36 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3025 3130 4034 4051 4204 4389 4834 Size: 1255 1445 105 904 17 153 185 445 Cuts at: 4834 325 Size: 1498 Fragments arranged by size: 1498 1445 1255 904 445 185 153 105 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4253 5965 96 Size: 274 80 33 51 1443 2276 1712 138 Fragments arranged by size: 2276 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3218 3250 5967 5999 3218 Size: 32 2717 32 3226 Fragments arranged by size: 3226 2717 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6007 XbaI T'CTAG_A Cuts at: 3343 3343 Size: 6007 XhoI C'TCGA_G Cuts at: 1943 2178 1943 Size: 235 5772 XmaI C'CCGG_G Cuts at: 1870 3332 1870 Size: 1462 4545 XmnI GAAnn'nnTTC Cuts at: 4136 4136 Size: 6007 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3815 276 Size: 53 83 186 936 2281 2468 Fragments arranged by size: 2468 2281 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AscI AvrII BaeI BbeI BbvCI BfrBI BsiWI BsmBI BstZ17I Bsu36I EcoICRI EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspXI PsrI PvuII RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI XcmI