################################## # NO CUT ENZYMES ( 128 enzymes) # ################################## Acc65I AccI AclI AgeI AhdI AlwNI AocI ApaI AscI AseI AsnI Asp700I Asp718I AspEI AvaII AviII AvrII BanI BbeI BbrPI BbsI BbvCI BcgI BciVI BcnI BfaI BglI BglII BlnI BpuAI BsaAI BsaBI BsaI BsiEI BsiWI BsmBI BsmI Bsp1407I BspDI BsrBI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstXI BstZ17I Bsu36I Cfr10I ClaI CpoI DraIII DrdI DsaI EagI Eam1105I EclXI Eco52I Eco81I EcoO65I EcoRI EcoRV EcoT22I FseI FspI HgaI HincII HindII HpaI KasI KpnI KspI MaeI MamI MfeI MunI NaeI NarI NciI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PinAI PleI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI RsrII SacI SacII SalI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI Sse8387I SspBI SspI StuI SwaI TspRI XbaI XcmI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 68 enzymes) # ################################### AatII 561 GACGTC GACGT^C AccII 203 CGCG CG^CG AccIII 790 TCCGGA T^CCGGA AcsI 322 AAATTT [GA]^AATT[CT] AcyI 561 GACGTC G[GA]^CG[CT]C AfaI 831 GTAC GT^AC AflII 42 CTTAAG C^TTAAG Alw44I 856 GTGCAC G^TGCAC Aor51HI 971 AGCGCT AGC^GCT ApaLI 856 GTGCAC G^TGCAC ApoI 322 AAATTT [GA]^AATT[CT] AspHI 856 GTGCAC G[AT]GC[AT]^C AspI 493 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC AvaI 440 CTCGAG C^[CT]CG[GA]G BamHI 384 GGATCC G^GATCC BanII 362 GAGCCC G[GA]GC[CT]^C BclI 327 TGATCA T^GATCA BfrI 42 CTTAAG C^TTAAG BlpI 757 GCTGAGC GC^T[ACGT]AGC Bpu1102I 757 GCTGAGC GC^T[ACGT]AGC BsaHI 561 GACGTC G[GA]^CG[CT]C BseAI 790 TCCGGA T^CCGGA BsgI 770 CTGCAC GTGCAG(16/14) BsiHKAI 856 GTGCAC G[AT]GC[AT]^C BsmAI 794 GAGAC GTCTC(1/5) BsmFI 59 GGGAC GGGAC(10/14) BsoBI 440 CTCGAG C^[CT]CG[GA]G BspEI 790 TCCGGA T^CCGGA BspHI 936 TCATGA T^CATGA BspLU11I 827 ACATGT A^CATGT BsrI 77 ACTGG ACTGG(1/-1) BstUI 203 CGCG CG^CG CelII 757 GCTGAGC GC^T[ACGT]AGC CfoI 972 GCGC GCG^C Cfr13I 51 GGCCC G^G[ACGT]CC DraI 610 TTTAAA TTT^AAA DraII 50 AGGCCCT [GA]G^G[ACGT]CC[CT] EarI 940 GAAGAG CTCTTC(1/4) Eco47III 971 AGCGCT AGC^GCT EcoO109I 50 AGGCCCT [GA]G^G[ACGT]CC[CT] FauI 639 CCCGC CCCGC(4/6) FbaI 327 TGATCA T^GATCA HaeII 971 AGCGCT [GA]GCGC^[CT] HhaI 972 GCGC GCG^C Hin1I 561 GACGTC G[GA]^CG[CT]C HinP1I 972 GCGC G^CGC Ksp632I 940 GAAGAG CTCTTC(1/4) MluI 202 ACGCGT A^CGCGT MroI 790 TCCGGA T^CCGGA MslI 423 CACAATGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MspA1I 518 CAGCTG C[AC]G^C[GT]G MvnI 203 CGCG CG^CG PaeR7I 440 CTCGAG C^TCGAG PflFI 493 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC PflMI 969 CCAGCGCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PstI 946 CTGCAG CTGCA^G PvuII 518 CAGCTG CAG^CTG RcaI 936 TCATGA T^CATGA RsaI 831 GTAC GT^AC SapI 940 GAAGAGC GCTCTTC(1/4) Sau96I 51 GGCCC G^G[ACGT]CC SfcI 946 CTGCAG C^T[GA][CT]AG SphI 784 GCATGC GCATG^C TaqI 441 TCGA T^CGA Tth111I 493 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC TthHB8I 441 TCGA T^CGA Van91I 969 CCAGCGCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XhoI 440 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 23 enzymes) # ################################### AflIII 202 ACGCGT A^C[GA][CT]GT 827 ACATGT BalI 191 TGGCCA TGG^CCA 253 TGGCCA BmyI 362 GAGCCC G[AGT]GC[ACT]^C 856 GTGCAC BpmI 695 CTCCAG CTGGAG(16/14) 22 CTGGAG BsaWI 790 TCCGGA [AT]^CCGG[AT] 819 TCCGGT BseRI 121 GAGGAG GAGGAG(10/8) 505 GAGGAG Bsp1286I 362 GAGCCC G[AGT]GC[ACT]^C 856 GTGCAC BspMI 804 ACCTGC ACCTGC(4/8) 852 GCAGGT BstF5I 870 CATCC GGATG(2/0) 928 CATCC BstYI 241 GGATCT [GA]^GATC[CT] 384 GGATCC DdeI 357 CTCAG C^T[ACGT]AG 758 CTGAG EcoNI 250 CCTTGGCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 352 CCTCTCTCAGG FokI 870 CATCC GGATG(9/13) 928 CATCC HapII 791 CCGG C^CGG 820 CCGG HpaII 791 CCGG C^CGG 820 CCGG MflI 241 GGATCT [GA]^GATC[CT] 384 GGATCC MluNI 191 TGGCCA TGG^CCA 253 TGGCCA MscI 191 TGGCCA TGG^CCA 253 TGGCCA MspI 791 CCGG C^CGG 820 CCGG NspI 784 GCATGC [GA]CATG^[CT] 827 ACATGT SmlI 42 CTTAAG C^T[CT][GA]AG 440 CTCGAG Tsp509I 323 AATT ^AATT 371 AATT XhoII 241 GGATCT [GA]^GATC[CT] 384 GGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 39 enzymes) # ######################################### AciI AluI AlwI BbvI BsaJI BsiYI BslI BssKI BstNI Cac8I DpnII EaeI EcoRII EcoT14I Fnu4HI HaeIII HindIII HinfI HphI ItaI MaeII MaeIII MboI MboII MnlI MseI MvaI MwoI NdeII NlaIII NlaIV Sau3AI ScrFI SfaNI StyI TfiI Tru9I TseI Tsp45I