(Circular) (Six-base) MAPSORT of: pFN21AE1821.seq Check: 699 from: 1 to: 5710 pFN21AE1821 5710 bp With 182 enzymes: SgfI * August 16, 2010 14:54 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2848 2848 Size: 5710 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2570 3520 278 Size: 53 83 186 936 1034 950 2468 Fragments arranged by size: 2468 1034 950 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3053 1937 Size: 1116 4594 Acc65I G'GTAC_C Cuts at: 3031 3031 Size: 5710 AclI AA'CG_TT Cuts at: 1563 3837 4210 1563 Size: 2274 373 3063 Fragments arranged by size: 3063 2274 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2678 3773 5157 5425 1632 Size: 1046 1095 1384 268 1917 Fragments arranged by size: 1917 1384 1095 1046 268 AfeI AGC'GCT Cuts at: 2978 2978 Size: 5710 AflII C'TTAA_G Cuts at: 820 1017 2047 820 Size: 197 1030 4483 Fragments arranged by size: 4483 1030 197 AflIII A'CryG_T Cuts at: 1203 2207 2832 4609 5586 1203 Size: 1004 625 1777 977 1327 Fragments arranged by size: 1777 1327 1004 977 625 AgeI A'CCGG_T Cuts at: 4541 4541 Size: 5710 AhdI GACnn_n'nnGTC Cuts at: 4439 4439 Size: 5710 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5710 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2790 1184 Size: 1606 4104 AlwNI CAG_nnn'CTG Cuts at: 3453 5025 3453 Size: 1572 4138 ApaLI G'TGCA_C Cuts at: 2861 3767 4923 2861 Size: 906 1156 3648 Fragments arranged by size: 3648 1156 906 ApoI r'AATT_y Cuts at: 1493 2327 3019 3245 3397 5506 1493 Size: 834 692 226 152 2109 1697 Fragments arranged by size: 2109 1697 834 692 226 152 AseI AT'TA_AT Cuts at: 160 1032 4264 160 Size: 872 3232 1606 Fragments arranged by size: 3232 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5710 AvaI C'yCGr_G Cuts at: 1870 1943 2445 3035 1870 Size: 73 502 590 4545 Fragments arranged by size: 4545 590 502 73 BamHI G'GATC_C Cuts at: 2389 3040 3407 2389 Size: 651 367 4692 Fragments arranged by size: 4692 651 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3031 4486 618 Size: 325 217 627 1244 1455 1842 Fragments arranged by size: 1842 1455 1244 627 325 217 BanII G_rGCy'C Cuts at: 1391 2371 3029 1391 Size: 980 658 4072 Fragments arranged by size: 4072 980 658 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4739 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3901 3935 1903 Size: 34 1964 34 3678 Fragments arranged by size: 3678 1964 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3602 4812 3602 Size: 1210 4500 BclI T'GATC_A Cuts at: 1548 2332 1548 Size: 784 4926 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4321 136 Size: 107 122 71 631 605 2649 1525 Fragments arranged by size: 2649 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5705 5705 Size: 5710 BlpI GC'TnA_GC Cuts at: 2763 3127 2763 Size: 364 5346 Bme1580I G_kGCm'C Cuts at: 1790 2865 3771 4927 1790 Size: 1075 906 1156 2573 Fragments arranged by size: 2573 1156 1075 906 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5710 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2092 3441 4399 Size: 416 53 83 399 24 675 1349 958 Cuts at: 4399 442 Size: 1753 Fragments arranged by size: 1753 1349 958 675 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5710 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2048 2685 4370 1239 Size: 809 637 1685 2579 Fragments arranged by size: 2579 1685 809 637 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3835 4700 4998 3835 Size: 865 298 4547 Fragments arranged by size: 4547 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4373 882 Size: 617 2874 2219 Fragments arranged by size: 2874 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3501 493 Size: 711 2297 2702 Fragments arranged by size: 2702 2297 711 BsaBI GATnn'nnATC Cuts at: 5704 5704 Size: 5710 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2567 3517 3899 4534 Size: 53 83 186 936 1034 950 382 635 Cuts at: 4534 275 Size: 1451 Fragments arranged by size: 1451 1034 950 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2795 2824 4143 4541 4815 4962 5373 2795 Size: 29 1319 398 274 147 411 3132 Fragments arranged by size: 3132 1319 411 398 274 147 29 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5485 Fragments arranged by size: 5485 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2141 2525 1186 Size: 955 384 4371 Fragments arranged by size: 4371 955 384 BseYI C'CCAG_C Cuts at: 2370 4913 5409 2370 Size: 2543 496 2671 Fragments arranged by size: 2671 2543 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2760 1163 Size: 573 1024 4113 Fragments arranged by size: 4113 1024 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3160 3921 4949 1540 Size: 275 186 1159 761 1028 2301 Fragments arranged by size: 2301 1159 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2865 3771 3856 4927 2865 Size: 906 85 1071 3648 Fragments arranged by size: 3648 1071 906 85 BsmI GAATG_Cn' Cuts at: 3221 3314 3221 Size: 93 5617 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2371 2865 3029 3771 3856 4927 1391 Size: 399 581 494 164 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 581 494 399 164 85 BspEI T'CCGG_A Cuts at: 2795 5373 2795 Size: 2578 3132 BspHI T'CATG_A Cuts at: 2941 3599 5329 2941 Size: 658 1730 3322 Fragments arranged by size: 3322 1730 658 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2818 2848 3065 844 Size: 1974 30 217 3489 Fragments arranged by size: 3489 1974 217 30 BsrBI CCG'CTC Cuts at: 1394 1823 3597 1394 Size: 429 1774 3507 Fragments arranged by size: 3507 1774 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4205 4379 65 Size: 1183 2957 174 1396 Fragments arranged by size: 2957 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4354 4541 1700 Size: 110 2544 187 2869 Fragments arranged by size: 2869 2544 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5710 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5710 BssSI C'ACGA_G Cuts at: 3770 4782 3770 Size: 1012 4698 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5710 BstBI TT'CG_AA Cuts at: 4514 4514 Size: 5710 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5710 BstYI r'GATC_y Cuts at: 1972 2246 2389 3040 3407 3792 3809 5261 5347 Size: 274 143 651 367 385 17 1452 86 Cuts at: 5347 5359 5705 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 651 385 367 346 274 143 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4219 Fragments arranged by size: 4219 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4971 Fragments arranged by size: 4971 683 56 BtsI GCAGTG_nn' Cuts at: 3226 4020 4040 3226 Size: 794 20 4896 Fragments arranged by size: 4896 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5710 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5675 DraI TTT'AAA Cuts at: 2617 3014 2617 Size: 397 5313 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4717 809 Size: 507 3401 1802 Fragments arranged by size: 3401 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2034 2196 2258 Size: 54 1425 50 275 18 204 162 62 Cuts at: 2258 3157 4046 5675 8 Size: 899 889 1629 43 Fragments arranged by size: 1629 1425 899 889 275 204 162 62 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3157 1537 Size: 275 1345 4090 Fragments arranged by size: 4090 1345 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2940 3640 1353 Size: 1587 700 3423 Fragments arranged by size: 3423 1587 700 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4297 4671 4817 387 Size: 1064 138 2708 374 146 1280 Fragments arranged by size: 2708 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2048 2678 2685 3773 4370 5157 5425 Size: 393 416 630 7 1088 597 787 268 Cuts at: 5425 1239 Size: 1524 Fragments arranged by size: 1524 1088 787 630 597 416 393 268 7 EcoNI CCTnn'n_nnAGG Cuts at: 2259 2361 2259 Size: 102 5608 EcoO109I rG'GnC_Cy Cuts at: 2056 2056 Size: 5710 EcoRI G'AATT_C Cuts at: 3019 3397 3019 Size: 378 5332 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2062 2607 2062 Size: 545 5165 FspI TGC'GCA Cuts at: 4216 4216 Size: 5710 HaeII r_GCGC'y Cuts at: 1678 2980 4857 1678 Size: 1302 1877 2531 Fragments arranged by size: 2531 1877 1302 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4351 4383 4425 4457 1373 Size: 32 135 32 2779 32 42 32 2626 Fragments arranged by size: 2779 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3054 3300 669 Size: 988 281 1116 246 3079 Fragments arranged by size: 3079 1116 988 281 246 HindIII A'AGCT_T Cuts at: 748 2306 2589 2850 748 Size: 1558 283 261 3608 Fragments arranged by size: 3608 1558 283 261 HpaI GTT'AAC Cuts at: 3300 3300 Size: 5710 KpnI G_GTAC'C Cuts at: 3035 3035 Size: 5710 MfeI C'AATT_G Cuts at: 3309 3309 Size: 5710 MluI A'CGCG_T Cuts at: 2207 5586 2207 Size: 3379 2331 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4824 5008 1305 Size: 3519 184 2007 Fragments arranged by size: 3519 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2198 2260 10 Size: 54 1425 343 366 62 3460 Fragments arranged by size: 3460 1425 366 343 62 54 MslI CAynn'nnrTG Cuts at: 518 1166 2432 3668 4027 4186 518 Size: 648 1266 1236 359 159 2042 Fragments arranged by size: 2042 1266 1236 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2525 3126 3803 4951 5196 1606 Size: 919 601 677 1148 245 2120 Fragments arranged by size: 2120 1148 919 677 601 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4219 Fragments arranged by size: 4219 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5710 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5710 NotI GC'GGCC_GC Cuts at: 3157 3157 Size: 5710 NspI r_CATG'y Cuts at: 1132 1229 2793 2836 3068 4613 1132 Size: 97 1564 43 232 1545 2229 Fragments arranged by size: 2229 1564 1545 232 97 43 PasI CC'CwG_GG Cuts at: 1348 2059 1348 Size: 711 4999 PciI A'CATG_T Cuts at: 2832 4609 2832 Size: 1777 3933 PflMI CCAn_nnn'nTGG Cuts at: 1108 2980 1108 Size: 1872 3838 PmeI GTTT'AAAC Cuts at: 3014 3014 Size: 5710 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3779 3811 5292 5324 3779 Size: 32 1481 32 4165 Fragments arranged by size: 4165 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5710 PsiI TTA'TAA Cuts at: 3280 3280 Size: 5710 PspXI vC'TCGA_Gb Cuts at: 2445 2445 Size: 5710 PstI C_TGCA'G Cuts at: 830 2955 3062 830 Size: 2125 107 3478 Fragments arranged by size: 3478 2125 107 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5710 PvuII CAG'CTG Cuts at: 2525 2525 Size: 5710 SalI G'TCGA_C Cuts at: 1936 3052 1936 Size: 1116 4594 SapI GCTCTTCn'nnn_ Cuts at: 2940 2940 Size: 5710 SbfI CC_TGCA'GG Cuts at: 3062 3062 Size: 5710 ScaI AGT'ACT Cuts at: 3958 5670 3958 Size: 1712 3998 SfcI C'TryA_G Cuts at: 826 1045 2951 3058 4193 4874 5065 826 Size: 219 1906 107 1135 681 191 1471 Fragments arranged by size: 1906 1471 1135 681 219 191 107 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5710 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5710 SmaI CCC'GGG Cuts at: 1872 3037 1872 Size: 1165 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2047 2445 3814 4715 4977 820 Size: 197 926 104 398 1369 901 262 1553 Fragments arranged by size: 1553 1369 926 901 398 262 197 104 SnaBI TAC'GTA Cuts at: 493 3501 493 Size: 3008 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5710 SphI G_CATG'C Cuts at: 1132 2793 3068 1132 Size: 1661 275 3774 Fragments arranged by size: 3774 1661 275 SspI AAT'ATT Cuts at: 5 52 3634 5 Size: 47 3582 2081 Fragments arranged by size: 3582 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5710 StyI C'CwwG_G Cuts at: 513 1065 2004 2255 2292 2988 3149 513 Size: 552 939 251 37 696 161 3074 Fragments arranged by size: 3074 939 696 552 251 161 37 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2760 3737 3754 3907 4092 4537 325 Size: 1255 1180 977 17 153 185 445 1498 Fragments arranged by size: 1498 1255 1180 977 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3956 5668 96 Size: 274 80 33 51 1443 1979 1712 138 Fragments arranged by size: 1979 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5670 5702 5670 Size: 32 5678 Tth111I GACn'n_nGTC Cuts at: 1316 2501 1316 Size: 1185 4525 XbaI T'CTAG_A Cuts at: 3046 3046 Size: 5710 XhoI C'TCGA_G Cuts at: 1943 2445 1943 Size: 502 5208 XmaI C'CCGG_G Cuts at: 1870 3035 1870 Size: 1165 4545 XmnI GAAnn'nnTTC Cuts at: 3839 3839 Size: 5710 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2568 3518 276 Size: 53 83 186 936 1034 950 2468 Fragments arranged by size: 2468 1034 950 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PspXI PstI PvuI PvuII SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PsrI RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI XcmI