################################## # NO CUT ENZYMES ( 199 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcsI AcyI AflII AgeI AhdI Alw44I AlwI AlwNI AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvaI AviII AvrII BalI BamHI BanI BbeI BbrPI BbsI BbvCI BcgI BclI BcnI BfrI BglI BglII BlnI BlpI BpmI Bpu1102I BpuAI BsaAI BsaBI BsaHI BsaI BsaWI BseAI BsgI BsiEI BsiWI BsmAI BsmBI BsmFI BsoBI BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrDI BsrFI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstF5I BstPI BstYI BstZ17I Bsu36I CelII CfoI Cfr10I ClaI CpoI DpnII DraI DraII DraIII DrdI DsaI EaeI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoO109I EcoO65I EcoRI EcoRV EcoT22I FbaI FokI FseI FspI HaeII HapII HgaI HhaI Hin1I HinP1I HincII HindII HindIII HinfI HpaI HpaII HphI KasI KpnI Ksp632I KspI MaeIII MamI MboI MfeI MflI MluI MluNI MroI MscI MspA1I MspI MunI NaeI NarI NciI NcoI NdeI NdeII NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PleI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsrII SacI SacII SalI SapI Sau3AI SbfI ScaI SexAI SfcI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspI StuI SwaI TfiI Tsp45I Tsp509I Tth111I Van91I XbaI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 27 enzymes) # ################################### AccII 248 CGCG CG^CG AflIII 188 ACACGT A^C[GA][CT]GT AspHI 390 GTGCTC G[AT]GC[AT]^C BseRI 341 GAGGAG GAGGAG(10/8) BsiHKAI 390 GTGCTC G[AT]GC[AT]^C BsmI 167 GCATTC GAATGC(1/-1) Bsp1407I 128 TGTACA T^GTACA BsrGI 128 TGTACA T^GTACA BsrI 40 ACTGG ACTGG(1/-1) BstUI 248 CGCG CG^CG BstXI 402 CCAGCATCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG DdeI 14 CTAAG C^T[ACGT]AG EcoNI 9 CCTCTCTAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoT14I 89 CCAAGG C^C[AT][AT]GG MaeII 190 ACGT A^CGT MboII 158 TCTTC GAAGA(8/7) MseI 419 TTAA T^TAA MslI 381 CACAACTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MvnI 248 CGCG CG^CG NlaIII 126 CATG CATG^ SfaNI 405 GCATC GCATC(5/9) SspBI 128 TGTACA T^GTACA StyI 89 CCAAGG C^C[AT][AT]GG TaqI 339 TCGA T^CGA Tru9I 419 TTAA T^TAA TspRI 435 CAGTG CA[GC]TG[ACGT][ACGT]^ TthHB8I 339 TCGA T^CGA ################################### # TWO CUT ENZYMES ( 14 enzymes) # ################################### AciI 96 CCGC CCGC(-3/-1) 247 CCGC AfaI 105 GTAC GT^AC 129 GTAC BanII 176 GAGCCC G[GA]GC[CT]^C 344 GAGCCC BbvI 46 GCTGC GCAGC(8/12) 449 GCTGC BciVI 117 GTATCC GTATCC(6/5) 201 GTATCC BfaI 73 CTAG C^TAG 223 CTAG FauI 95 CCCGC CCCGC(4/6) 246 CCCGC Fnu4HI 46 GCTGC GC^[ACGT]GC 449 GCTGC ItaI 46 GCTGC GC^[ACGT]GC 449 GCTGC MaeI 73 CTAG C^TAG 223 CTAG MwoI 67 GCCCACCTAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC 371 GCTTGGTGGGC RsaI 105 GTAC GT^AC 129 GTAC TseI 46 GCTGC G^C[AT]GC 449 GCTGC XcmI 50 CCAGCTTTATAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 356 CCATCGCCCTCCTGG ######################################### # 3 OR MORE CUT ENZYMES ( 18 enzymes) # ######################################### AluI AvaII BmyI BsaJI BsiYI BslI Bsp1286I BssKI BstNI Cac8I Cfr13I EcoRII HaeIII MnlI MvaI NlaIV Sau96I ScrFI