################################## # NO CUT ENZYMES ( 109 enzymes) # ################################## Acc65I AccI AccII AccIII AclI AgeI AhdI Alw44I Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvaI AviII AvrII BbsI BbvCI BcgI BlnI BpuAI BseAI BsgI BsiWI BsmBI BsoBI BspDI BspEI BspHI BsrFI BssHII Bst1107I BstBI BstEII BstPI BstUI BstZ17I Cfr10I ClaI CpoI DraI DrdI EagI Eam1105I EclXI Eco47III Eco52I EcoNI EcoO65I EcoRI EcoRV EcoT22I FseI FspI HgaI HindIII HpaI KpnI KspI MluI MroI MvnI NaeI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PmeI PshAI PshBI Psp1406I RcaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspI StuI SwaI Tth111I XbaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 45 enzymes) # ################################### AatII 976 GACGTC GACGT^C AflII 1867 CTTAAG C^TTAAG AocI 1543 CCTGAGG CC^T[ACGT]AGG BanII 2008 GAGCCC G[GA]GC[CT]^C BbeI 311 GGCGCC GGCGC^C BbrPI 745 CACGTG CAC^GTG BciVI 664 GGATAC GTATCC(6/5) BclI 1955 TGATCA T^GATCA BfrI 1867 CTTAAG C^TTAAG BglI 1449 GCCATCCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BlpI 2484 GCTCAGC GC^T[ACGT]AGC Bpu1102I 2484 GCTCAGC GC^T[ACGT]AGC BsaAI 745 CACGTG [CT]AC^GT[GA] BsaI 2417 GAGACC GGTCTC(1/5) BsiEI 1413 CGATCG CG[GA][CT]^CG BspLU11I 869 ACATGT A^CATGT BspMI 1637 ACCTGC ACCTGC(4/8) BsrDI 1105 GCAATG GCAATG(2/0) BssSI 2005 CACGAG CACGAG(-5/-1) BstAPI 1275 GCAGAAAATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstXI 1530 CCAGCCCAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Bsu36I 1543 CCTGAGG CC^T[ACGT]AGG CelII 2484 GCTCAGC GC^T[ACGT]AGC CfoI 312 GCGC GCG^C DraIII 1382 CACCTAGTG CAC[ACGT][ACGT][ACGT]^GTG DsaI 1631 CCATGG C^C[GA][CT]GG EarI 2498 GAAGAG CTCTTC(1/4) Eco81I 1543 CCTGAGG CC^T[ACGT]AGG FauI 2000 CCCGC CCCGC(4/6) FbaI 1955 TGATCA T^GATCA HaeII 311 GGCGCC [GA]GCGC^[CT] HhaI 312 GCGC GCG^C HinP1I 312 GCGC G^CGC HincII 979 GTCAAC GT[CT]^[GA]AC HindII 979 GTCAAC GT[CT]^[GA]AC KasI 311 GGCGCC G^GCGCC Ksp632I 2498 GAAGAG CTCTTC(1/4) MfeI 691 CAATTG C^AATTG MunI 691 CAATTG C^AATTG NarI 311 GGCGCC GG^CGCC NcoI 1631 CCATGG C^CATGG PmaCI 745 CACGTG CAC^GTG PmlI 745 CACGTG CAC^GTG PvuI 1413 CGATCG CGAT^CG SfoI 311 GGCGCC GGC^GCC ################################### # TWO CUT ENZYMES ( 23 enzymes) # ################################### AcyI 311 GGCGCC G[GA]^CG[CT]C 976 GACGTC AflIII 746 ACGTGT A^C[GA][CT]GT 869 ACATGT AspHI 989 GTGCTC G[AT]GC[AT]^C 2242 GTGCTC BamHI 1196 GGATCC G^GATCC 2225 GGATCC BcnI 754 CCCGG CC^[GC]GG 2235 CCGGG BsaBI 1875 GATAAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 1981 GATCTCAATC BsaHI 311 GGCGCC G[GA]^CG[CT]C 976 GACGTC BsaWI 491 TCCGGT [AT]^CCGG[AT] 2222 ACCGGA BsiHKAI 989 GTGCTC G[AT]GC[AT]^C 2242 GTGCTC BsmFI 301 GGGAC GGGAC(10/14) 2516 GTCCC BsmI 17 GAATGC GAATGC(1/-1) 1848 GAATGC BsrBI 918 CCGCTC CCGCTC(-3/-3) 1371 CCGCTC Cac8I 773 GCTGGC GC[ACGT]^[ACGT]GC 1620 GCCTGC DraII 301 GGGACCC [GA]G^G[ACGT]CC[CT] 1898 AGGACCC EcoO109I 301 GGGACCC [GA]G^G[ACGT]CC[CT] 1898 AGGACCC Hin1I 311 GGCGCC G[GA]^CG[CT]C 976 GACGTC MaeII 746 ACGT A^CGT 977 ACGT MamI 1875 GATAAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 1981 GATCTCAATC NciI 754 CCCGG CC^[GC]GG 2235 CCGGG PpuMI 301 GGGACCC [GA]G^G[AT]CC[CT] 1898 AGGACCC PstI 259 CTGCAG CTGCA^G 1506 CTGCAG TaqI 335 TCGA T^CGA 1405 TCGA TthHB8I 335 TCGA T^CGA 1405 TCGA ######################################### # 3 OR MORE CUT ENZYMES ( 81 enzymes) # ######################################### AciI AcsI AfaI AluI AlwI AlwNI ApoI AvaII BalI BanI BbvI BfaI BglII BmyI BpmI BsaJI BseRI BsiYI BslI BsmAI Bsp1286I Bsp1407I BsrGI BsrI BssKI BstF5I BstNI BstYI Cfr13I DdeI DpnII EaeI EcoRII EcoT14I Fnu4HI FokI HaeIII HapII HinfI HpaII HphI ItaI MaeI MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MwoI NdeII NlaIII NlaIV NspI PflMI PleI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI SspBI StyI TfiI Tru9I TseI Tsp45I Tsp509I TspRI Van91I XcmI XhoII