(Circular) (Six-base) MAPSORT of: pFN21AE3314.seq Check: 4527 from: 1 to: 7270 pFN21AE3314 7270 bp With 182 enzymes: SgfI * January 30, 2014 17:24 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2985 5080 278 Size: 53 83 186 936 1449 2095 2468 Fragments arranged by size: 2468 2095 1449 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4613 1937 Size: 2676 4594 Acc65I G'GTAC_C Cuts at: 4591 4591 Size: 7270 AclI AA'CG_TT Cuts at: 1563 5397 5770 1563 Size: 3834 373 3063 Fragments arranged by size: 3834 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2273 2949 3147 4085 5333 6717 6985 1632 Size: 641 676 198 938 1248 1384 268 1917 Fragments arranged by size: 1917 1384 1248 938 676 641 268 198 AflII C'TTAA_G Cuts at: 820 1017 3872 820 Size: 197 2855 4218 Fragments arranged by size: 4218 2855 197 AflIII A'CryG_T Cuts at: 1203 2751 2874 6169 7146 1203 Size: 1548 123 3295 977 1327 Fragments arranged by size: 3295 1548 1327 977 123 AgeI A'CCGG_T Cuts at: 6101 6101 Size: 7270 AhdI GACnn_n'nnGTC Cuts at: 5999 5999 Size: 7270 AleI CACnn'nnGTG Cuts at: 1166 2300 1166 Size: 1134 6136 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7270 AlwNI CAG_nnn'CTG Cuts at: 2425 3107 3575 3626 4497 5013 6585 2425 Size: 682 468 51 871 516 1572 3110 Fragments arranged by size: 3110 1572 871 682 516 468 51 ApaLI G'TGCA_C Cuts at: 5327 6483 5327 Size: 1156 6114 ApoI r'AATT_y Cuts at: 1493 2825 2842 3482 3764 4579 4805 4957 7066 Size: 1332 17 640 282 815 226 152 2109 Cuts at: 7066 1493 Size: 1697 Fragments arranged by size: 2109 1697 1332 815 640 282 226 152 17 AseI AT'TA_AT Cuts at: 160 1032 5824 160 Size: 872 4792 1606 Fragments arranged by size: 4792 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7270 AvaI C'yCGr_G Cuts at: 1870 1943 4595 1870 Size: 73 2652 4545 Fragments arranged by size: 4545 2652 73 BamHI G'GATC_C Cuts at: 3201 4230 4600 4967 3201 Size: 1029 370 367 5504 Fragments arranged by size: 5504 1029 370 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2220 2316 3010 3151 3556 Size: 325 217 627 433 96 694 141 405 Cuts at: 3556 4591 6046 618 Size: 1035 1455 1842 Fragments arranged by size: 1842 1455 1035 694 627 433 405 325 217 141 96 BanII G_rGCy'C Cuts at: 1391 4017 4589 1391 Size: 2626 572 4072 Fragments arranged by size: 4072 2626 572 BbeI G_GCGC'C Cuts at: 2320 2320 Size: 7270 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 6299 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5461 5495 1903 Size: 34 3524 34 3678 Fragments arranged by size: 3678 3524 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2663 5162 6372 2663 Size: 2499 1210 3561 Fragments arranged by size: 3561 2499 1210 BclI T'GATC_A Cuts at: 1548 3960 1548 Size: 2412 4858 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3460 5881 136 Size: 107 122 71 631 605 1788 2421 1525 Fragments arranged by size: 2421 1788 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2895 3985 4132 7265 2895 Size: 1090 147 3133 2900 Fragments arranged by size: 3133 2900 1090 147 BlpI GC'TnA_GC Cuts at: 4490 4687 4490 Size: 197 7073 Bme1580I G_kGCm'C Cuts at: 1790 2225 3561 5331 6487 1790 Size: 435 1336 1770 1156 2573 Fragments arranged by size: 2573 1770 1336 1156 435 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7270 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2985 3534 5001 Size: 416 53 83 399 24 1568 549 1467 Cuts at: 5001 5959 442 Size: 958 1753 Fragments arranged by size: 1753 1568 1467 958 549 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7270 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2577 2640 3286 3311 3474 4015 4428 4519 Size: 1338 63 646 25 163 541 413 91 Cuts at: 4519 5930 1239 Size: 1411 2579 Fragments arranged by size: 2579 1411 1338 646 541 413 163 91 63 25 Bpu10I CC'TnA_GC Cuts at: 2073 2073 Size: 7270 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2077 3884 5395 6260 6558 2077 Size: 1807 1511 865 298 2789 Fragments arranged by size: 2789 1807 1511 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4416 5933 882 Size: 617 2917 1517 2219 Fragments arranged by size: 2917 2219 1517 617 BsaAI yAC'GTr Cuts at: 493 1204 2752 5061 493 Size: 711 1548 2309 2702 Fragments arranged by size: 2702 2309 1548 711 BsaBI GATnn'nnATC Cuts at: 3884 3990 7264 3884 Size: 106 3274 3890 Fragments arranged by size: 3890 3274 106 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2317 2982 5077 5459 Size: 53 83 186 936 784 665 2095 382 Cuts at: 5459 6094 275 Size: 635 1451 Fragments arranged by size: 2095 1451 936 784 665 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2496 4227 5703 6101 6375 6522 6933 2496 Size: 1731 1476 398 274 147 411 2833 Fragments arranged by size: 2833 1731 1476 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2897 2927 3459 3488 3489 Size: 30 165 30 1494 30 532 29 1 Cuts at: 3489 3518 1178 Size: 29 4930 Fragments arranged by size: 4930 1494 532 165 30 30 30 29 29 1 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3370 3512 3515 3686 1186 Size: 2184 142 3 171 4770 Fragments arranged by size: 4770 2184 171 142 3 BseYI C'CCAG_C Cuts at: 2597 3709 4285 4374 6473 6969 2597 Size: 1112 576 89 2099 496 2898 Fragments arranged by size: 2898 2099 1112 576 496 89 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 6697 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3421 4720 5481 6509 1540 Size: 275 186 1420 1299 761 1028 2301 Fragments arranged by size: 2301 1420 1299 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2998 4251 5331 5416 6487 2998 Size: 1253 1080 85 1071 3781 Fragments arranged by size: 3781 1253 1080 1071 85 BsmI GAATG_Cn' Cuts at: 2028 3859 4781 4874 2028 Size: 1831 922 93 4424 Fragments arranged by size: 4424 1831 922 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2225 2998 3561 4017 4251 4589 5331 Size: 399 435 773 563 456 234 338 742 Cuts at: 5331 5416 6487 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 773 742 563 456 435 399 338 234 85 BspEI T'CCGG_A Cuts at: 6933 6933 Size: 7270 BspHI T'CATG_A Cuts at: 5159 6889 5159 Size: 1730 5540 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3651 4625 844 Size: 2807 974 3489 Fragments arranged by size: 3489 2807 974 BsrBI CCG'CTC Cuts at: 1394 1823 2925 3378 5157 1394 Size: 429 1102 453 1779 3507 Fragments arranged by size: 3507 1779 1102 453 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3117 5765 5939 65 Size: 1183 1869 2648 174 1396 Fragments arranged by size: 2648 1869 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5914 6101 1700 Size: 110 4104 187 2869 Fragments arranged by size: 4104 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 2355 3114 4209 96 Size: 2259 759 1095 3157 Fragments arranged by size: 3157 2259 1095 759 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7270 BssSI C'ACGA_G Cuts at: 4010 5330 6342 4010 Size: 1320 1012 4938 Fragments arranged by size: 4938 1320 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 3286 794 Size: 2492 4778 BstBI TT'CG_AA Cuts at: 6074 6074 Size: 7270 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7270 BstXI CCAn_nnnn'nTGG Cuts at: 3542 3542 Size: 7270 BstYI r'GATC_y Cuts at: 1972 2391 2895 3201 3985 4132 4230 4600 4967 Size: 419 504 306 784 147 98 370 367 Cuts at: 4967 5352 5369 6821 6907 6919 7265 1972 Size: 385 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 784 504 419 385 370 367 346 306 147 98 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3549 3549 Size: 7270 BtgI C'CryG_G Cuts at: 513 1065 3636 513 Size: 552 2571 4147 Fragments arranged by size: 4147 2571 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 4050 487 Size: 683 56 2824 3707 Fragments arranged by size: 3707 2824 683 56 BtsI GCAGTG_nn' Cuts at: 2863 4251 4786 5580 5600 2863 Size: 1388 535 794 20 4533 Fragments arranged by size: 4533 1388 794 535 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7270 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7235 DraI TTT'AAA Cuts at: 4574 4574 Size: 7270 DraIII CAC_nnn'GTG Cuts at: 3392 3392 Size: 7270 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6277 809 Size: 507 4961 1802 Fragments arranged by size: 4961 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3030 3222 3243 Size: 54 1425 50 275 18 1200 192 21 Cuts at: 3243 4137 4717 5606 7235 8 Size: 894 580 889 1629 43 Fragments arranged by size: 1629 1425 1200 894 889 580 275 192 54 50 43 21 18 EagI C'GGCC_G Cuts at: 1537 1812 4717 1537 Size: 275 2905 4090 Fragments arranged by size: 4090 2905 275 EarI CTCTTCn'nnn_ Cuts at: 1353 4498 5200 1353 Size: 3145 702 3423 Fragments arranged by size: 3423 3145 702 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2416 2797 5857 6231 6377 387 Size: 1064 138 827 381 3060 374 146 1280 Fragments arranged by size: 3060 1280 1064 827 381 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2273 2577 2640 2949 3147 3286 3311 Size: 393 641 304 63 309 198 139 25 Cuts at: 3311 3474 4015 4085 4428 4519 5333 5930 6717 Size: 163 541 70 343 91 814 597 787 Cuts at: 6717 6985 1239 Size: 268 1524 Fragments arranged by size: 1524 814 787 641 597 541 393 343 309 304 268 198 163 139 91 70 63 25 EcoO109I rG'GnC_Cy Cuts at: 2307 3904 2307 Size: 1597 5673 EcoRI G'AATT_C Cuts at: 4579 4957 4579 Size: 378 6892 FspI TGC'GCA Cuts at: 5776 5776 Size: 7270 HaeII r_GCGC'y Cuts at: 1678 2320 6417 1678 Size: 642 4097 2531 Fragments arranged by size: 4097 2531 642 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2613 2645 2696 2728 3403 Size: 32 135 32 1041 32 51 32 675 Cuts at: 3403 3435 3488 3520 3632 3664 3877 3909 5911 Size: 32 53 32 112 32 213 32 2002 Cuts at: 5911 5943 5985 6017 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 2002 1041 675 213 135 112 53 51 42 32 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2986 4614 4860 669 Size: 988 281 1048 1628 246 3079 Fragments arranged by size: 3079 1628 1048 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 7270 HpaI GTT'AAC Cuts at: 4860 4860 Size: 7270 KasI G'GCGC_C Cuts at: 2316 2316 Size: 7270 KpnI G_GTAC'C Cuts at: 4595 4595 Size: 7270 MfeI C'AATT_G Cuts at: 2696 4869 2696 Size: 2173 5097 MluI A'CGCG_T Cuts at: 7146 7146 Size: 7270 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2647 3429 6384 6568 1305 Size: 1342 782 2955 184 2007 Fragments arranged by size: 2955 2007 1342 782 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3032 3224 3245 4139 10 Size: 54 1425 343 1200 192 21 894 3141 Fragments arranged by size: 3141 1425 1200 894 343 192 54 21 MslI CAynn'nnrTG Cuts at: 518 1166 2300 2528 2852 3741 5228 5587 5746 Size: 648 1134 228 324 889 1487 359 159 Cuts at: 5746 518 Size: 2042 Fragments arranged by size: 2042 1487 1134 889 648 359 324 228 159 MspA1I CmG'CkG Cuts at: 1606 2174 2597 3516 4260 4378 4494 4686 5363 Size: 568 423 919 744 118 116 192 677 Cuts at: 5363 6511 6756 1606 Size: 1148 245 2120 Fragments arranged by size: 2120 1148 919 744 677 568 423 245 192 118 116 NarI GG'CG_CC Cuts at: 2317 2317 Size: 7270 NcoI C'CATG_G Cuts at: 513 1065 3636 513 Size: 552 2571 4147 Fragments arranged by size: 4147 2571 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 7270 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7270 NotI GC'GGCC_GC Cuts at: 4717 4717 Size: 7270 NspI r_CATG'y Cuts at: 1132 1229 2113 2878 3364 4628 6173 1132 Size: 97 884 765 486 1264 1545 2229 Fragments arranged by size: 2229 1545 1264 884 765 486 97 PasI CC'CwG_GG Cuts at: 1348 2311 1348 Size: 963 6307 PciI A'CATG_T Cuts at: 2874 6169 2874 Size: 3295 3975 PflMI CCAn_nnn'nTGG Cuts at: 1108 2157 2940 3028 1108 Size: 1049 783 88 5350 Fragments arranged by size: 5350 1049 783 88 PfoI T'CCnGG_A Cuts at: 2973 2973 Size: 7270 PmeI GTTT'AAAC Cuts at: 4574 4574 Size: 7270 PmlI CAC'GTG Cuts at: 2752 2752 Size: 7270 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4508 4540 5339 5371 6852 6884 4508 Size: 32 799 32 1481 32 4894 Fragments arranged by size: 4894 1481 799 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2307 3904 2307 Size: 1597 5673 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7270 PsiI TTA'TAA Cuts at: 4840 4840 Size: 7270 PstI C_TGCA'G Cuts at: 830 2268 3515 4622 830 Size: 1438 1247 1107 3478 Fragments arranged by size: 3478 1438 1247 1107 PvuI CG_AT'CG Cuts at: 2001 3421 2001 Size: 1420 5850 PvuII CAG'CTG Cuts at: 2174 2597 4378 4494 2174 Size: 423 1781 116 4950 Fragments arranged by size: 4950 1781 423 116 SalI G'TCGA_C Cuts at: 1936 4612 1936 Size: 2676 4594 SanDI GG'GwC_CC Cuts at: 2307 2307 Size: 7270 SbfI CC_TGCA'GG Cuts at: 4622 4622 Size: 7270 ScaI AGT'ACT Cuts at: 5518 7230 5518 Size: 1712 5558 SfcI C'TryA_G Cuts at: 826 1045 2264 3511 4299 4618 5753 6434 6625 Size: 219 1219 1247 788 319 1135 681 191 Cuts at: 6625 826 Size: 1471 Fragments arranged by size: 1471 1247 1219 1135 788 681 319 219 191 SfoI GGC'GCC Cuts at: 2318 2318 Size: 7270 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7270 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7270 SmaI CCC'GGG Cuts at: 1872 4597 1872 Size: 2725 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2056 3872 3899 5374 6275 6537 Size: 197 926 113 1816 27 1475 901 262 Cuts at: 6537 820 Size: 1553 Fragments arranged by size: 1816 1553 1475 926 901 262 197 113 27 SnaBI TAC'GTA Cuts at: 493 5061 493 Size: 4568 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7270 SphI G_CATG'C Cuts at: 1132 4628 1132 Size: 3496 3774 SspI AAT'ATT Cuts at: 5 52 5194 5 Size: 47 5142 2081 Fragments arranged by size: 5142 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 7270 StyI C'CwwG_G Cuts at: 513 1065 3592 3636 4426 4709 513 Size: 552 2527 44 790 283 3074 Fragments arranged by size: 3074 2527 790 552 283 44 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2717 2747 3169 5297 5314 5467 5652 Size: 1255 1137 30 422 2128 17 153 185 Cuts at: 5652 6097 325 Size: 445 1498 Fragments arranged by size: 2128 1498 1255 1137 445 422 185 153 30 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2355 2611 3114 Size: 274 80 33 51 1443 378 256 503 Cuts at: 3114 4209 5516 7228 96 Size: 1095 1307 1712 138 Fragments arranged by size: 1712 1443 1307 1095 503 378 274 256 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2776 2808 2897 2929 3645 3677 7230 7262 2776 Size: 32 89 32 716 32 3553 32 2784 Fragments arranged by size: 3553 2784 716 89 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7270 XbaI T'CTAG_A Cuts at: 4606 4606 Size: 7270 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2026 2702 3145 4088 2026 Size: 676 443 943 5208 Fragments arranged by size: 5208 943 676 443 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7270 XmaI C'CCGG_G Cuts at: 1870 4595 1870 Size: 2725 4545 XmnI GAAnn'nnTTC Cuts at: 5399 5399 Size: 7270 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2983 5078 276 Size: 53 83 186 936 1449 2095 2468 Fragments arranged by size: 2468 2095 1449 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SanDI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbvCI BfrBI BplI BsiWI BsmBI BstZ17I EcoICRI EcoNI EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PspOMI PspXI PsrI RsrII SacI SacII SapI SexAI SfiI SrfI SwaI