(Linear) (Six-base) MAPSORT of: KIEE3314.seq Check: 5127 from: 1 to: 2565 KIEE3314.orf With 182 enzymes: SgfI * January 29, 2014 11:45 .. AatII G_ACGT'C Cuts at: 0 980 2565 Size: 980 1585 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 268 944 1142 2080 2565 Size: 268 676 198 938 485 Fragments arranged by size: 938 676 485 268 198 AflII C'TTAA_G Cuts at: 0 1867 2565 Size: 1867 698 AflIII A'CryG_T Cuts at: 0 746 869 2565 Size: 746 123 1696 Fragments arranged by size: 1696 746 123 AleI CACnn'nnGTG Cuts at: 0 295 2565 Size: 295 2270 AlwNI CAG_nnn'CTG Cuts at: 0 420 1102 1570 1621 2492 2565 Size: 420 682 468 51 871 73 Fragments arranged by size: 871 682 468 420 73 51 ApoI r'AATT_y Cuts at: 0 820 837 1477 1759 2565 Size: 820 17 640 282 806 Fragments arranged by size: 820 806 640 282 17 BamHI G'GATC_C Cuts at: 0 1196 2225 2565 Size: 1196 1029 340 Fragments arranged by size: 1196 1029 340 BanI G'GyrC_C Cuts at: 0 215 311 1005 1146 1551 2565 Size: 215 96 694 141 405 1014 Fragments arranged by size: 1014 694 405 215 141 96 BanII G_rGCy'C Cuts at: 0 2012 2565 Size: 2012 553 BbeI G_GCGC'C Cuts at: 0 315 2565 Size: 315 2250 BciVI GTATCCnnnnn_n' Cuts at: 0 658 2565 Size: 658 1907 BclI T'GATC_A Cuts at: 0 1955 2565 Size: 1955 610 BglI GCCn_nnn'nGGC Cuts at: 0 1455 2565 Size: 1455 1110 BglII A'GATC_T Cuts at: 0 890 1980 2127 2565 Size: 890 1090 147 438 Fragments arranged by size: 1090 890 438 147 BlpI GC'TnA_GC Cuts at: 0 2485 2565 Size: 2485 80 Bme1580I G_kGCm'C Cuts at: 0 220 1556 2565 Size: 220 1336 1009 Fragments arranged by size: 1336 1009 220 BmrI ACTGGGnnnn_n' Cuts at: 0 980 1529 2565 Size: 980 549 1036 Fragments arranged by size: 1036 980 549 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 572 635 1281 1306 1469 2010 2423 2514 Size: 572 63 646 25 163 541 413 91 Cuts at: 2514 2565 Size: 51 Fragments arranged by size: 646 572 541 413 163 91 63 51 25 Bpu10I CC'TnA_GC Cuts at: 0 68 2565 Size: 68 2497 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 72 1879 2565 Size: 72 1807 686 Fragments arranged by size: 1807 686 72 BsaI GGTCTCn'nnnn_ Cuts at: 0 2411 2565 Size: 2411 154 BsaAI yAC'GTr Cuts at: 0 747 2565 Size: 747 1818 BsaBI GATnn'nnATC Cuts at: 0 1879 1985 2565 Size: 1879 106 580 Fragments arranged by size: 1879 580 106 BsaHI Gr'CG_yC Cuts at: 0 312 977 2565 Size: 312 665 1588 Fragments arranged by size: 1588 665 312 BsaWI w'CCGG_w Cuts at: 0 491 2222 2565 Size: 491 1731 343 Fragments arranged by size: 1731 491 343 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 892 922 1454 1483 1484 1513 2565 Size: 892 30 532 29 1 29 1052 Fragments arranged by size: 1052 892 532 30 29 29 1 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 1365 1507 1510 1681 2565 Size: 1365 142 3 171 884 Fragments arranged by size: 1365 884 171 142 3 BseYI C'CCAG_C Cuts at: 0 592 1704 2280 2369 2565 Size: 592 1112 576 89 196 Fragments arranged by size: 1112 592 576 196 89 BsiEI CG_ry'CG Cuts at: 0 1416 2565 Size: 1416 1149 BsiHKAI G_wGCw'C Cuts at: 0 993 2246 2565 Size: 993 1253 319 Fragments arranged by size: 1253 993 319 BsmI GAATG_Cn' Cuts at: 0 23 1854 2565 Size: 23 1831 711 Fragments arranged by size: 1831 711 23 Bsp1286I G_dGCh'C Cuts at: 0 220 993 1556 2012 2246 2565 Size: 220 773 563 456 234 319 Fragments arranged by size: 773 563 456 319 234 220 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 1646 2565 Size: 1646 919 BsrBI CCG'CTC Cuts at: 0 920 1373 2565 Size: 920 453 1192 Fragments arranged by size: 1192 920 453 BsrDI GCAATG_nn' Cuts at: 0 1112 2565 Size: 1112 1453 BsrGI T'GTAC_A Cuts at: 0 350 1109 2204 2565 Size: 350 759 1095 361 Fragments arranged by size: 1095 759 361 350 BssSI C'ACGA_G Cuts at: 0 2005 2565 Size: 2005 560 BstAPI GCAn_nnn'nTGC Cuts at: 0 1281 2565 Size: 1281 1284 BstXI CCAn_nnnn'nTGG Cuts at: 0 1537 2565 Size: 1537 1028 BstYI r'GATC_y Cuts at: 0 386 890 1196 1980 2127 2225 2565 Size: 386 504 306 784 147 98 340 Fragments arranged by size: 784 504 386 340 306 147 98 Bsu36I CC'TnA_GG Cuts at: 0 1544 2565 Size: 1544 1021 BtgI C'CryG_G Cuts at: 0 1631 2565 Size: 1631 934 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 2045 2565 Size: 2045 520 BtsI GCAGTG_nn' Cuts at: 0 858 2246 2565 Size: 858 1388 319 Fragments arranged by size: 1388 858 319 DraIII CAC_nnn'GTG Cuts at: 0 1387 2565 Size: 1387 1178 EaeI y'GGCC_r Cuts at: 0 1025 1217 1238 2132 2565 Size: 1025 192 21 894 433 Fragments arranged by size: 1025 894 433 192 21 EarI CTCTTCn'nnn_ Cuts at: 0 2493 2565 Size: 2493 72 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 411 792 2565 Size: 411 381 1773 Fragments arranged by size: 1773 411 381 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 268 572 635 944 1142 1281 1306 1469 Size: 268 304 63 309 198 139 25 163 Cuts at: 1469 2010 2080 2423 2514 2565 Size: 541 70 343 91 51 Fragments arranged by size: 541 343 309 304 268 198 163 139 91 70 63 51 25 EcoO109I rG'GnC_Cy Cuts at: 0 302 1899 2565 Size: 302 1597 666 Fragments arranged by size: 1597 666 302 HaeII r_GCGC'y Cuts at: 0 315 2565 Size: 315 2250 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 608 640 691 723 1398 1430 1483 1515 Size: 608 32 51 32 675 32 53 32 Cuts at: 1515 1627 1659 1872 1904 2565 Size: 112 32 213 32 661 Fragments arranged by size: 675 661 608 213 112 53 51 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 0 981 2565 Size: 981 1584 KasI G'GCGC_C Cuts at: 0 311 2565 Size: 311 2254 MfeI C'AATT_G Cuts at: 0 691 2565 Size: 691 1874 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 642 1424 2565 Size: 642 782 1141 Fragments arranged by size: 1141 782 642 MscI TGG'CCA Cuts at: 0 1027 1219 1240 2134 2565 Size: 1027 192 21 894 431 Fragments arranged by size: 1027 894 431 192 21 MslI CAynn'nnrTG Cuts at: 0 295 523 847 1736 2565 Size: 295 228 324 889 829 Fragments arranged by size: 889 829 324 295 228 MspA1I CmG'CkG Cuts at: 0 169 592 1511 2255 2373 2489 2565 Size: 169 423 919 744 118 116 76 Fragments arranged by size: 919 744 423 169 118 116 76 NarI GG'CG_CC Cuts at: 0 312 2565 Size: 312 2253 NcoI C'CATG_G Cuts at: 0 1631 2565 Size: 1631 934 NspI r_CATG'y Cuts at: 0 108 873 1359 2565 Size: 108 765 486 1206 Fragments arranged by size: 1206 765 486 108 PasI CC'CwG_GG Cuts at: 0 306 2565 Size: 306 2259 PciI A'CATG_T Cuts at: 0 869 2565 Size: 869 1696 PflMI CCAn_nnn'nTGG Cuts at: 0 152 935 1023 2565 Size: 152 783 88 1542 Fragments arranged by size: 1542 783 152 88 PfoI T'CCnGG_A Cuts at: 0 968 2565 Size: 968 1597 PmlI CAC'GTG Cuts at: 0 747 2565 Size: 747 1818 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 2503 2535 2565 Size: 2503 32 30 Fragments arranged by size: 2503 32 30 PpuMI rG'GwC_Cy Cuts at: 0 302 1899 2565 Size: 302 1597 666 Fragments arranged by size: 1597 666 302 PstI C_TGCA'G Cuts at: 0 263 1510 2565 Size: 263 1247 1055 Fragments arranged by size: 1247 1055 263 PvuI CG_AT'CG Cuts at: 0 1416 2565 Size: 1416 1149 PvuII CAG'CTG Cuts at: 0 169 592 2373 2489 2565 Size: 169 423 1781 116 76 Fragments arranged by size: 1781 423 169 116 76 SanDI GG'GwC_CC Cuts at: 0 302 2565 Size: 302 2263 SfcI C'TryA_G Cuts at: 0 259 1506 2294 2565 Size: 259 1247 788 271 Fragments arranged by size: 1247 788 271 259 SfoI GGC'GCC Cuts at: 0 313 2565 Size: 313 2252 SmlI C'TyrA_G Cuts at: 0 51 1867 1894 2565 Size: 51 1816 27 671 Fragments arranged by size: 1816 671 51 27 StyI C'CwwG_G Cuts at: 0 1587 1631 2421 2565 Size: 1587 44 790 144 Fragments arranged by size: 1587 790 144 44 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 712 742 1164 2565 Size: 712 30 422 1401 Fragments arranged by size: 1401 712 422 30 TatI w'GTAC_w Cuts at: 0 350 606 1109 2204 2565 Size: 350 256 503 1095 361 Fragments arranged by size: 1095 503 361 350 256 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 771 803 892 924 1640 1672 2565 Size: 771 32 89 32 716 32 893 Fragments arranged by size: 893 771 716 89 32 32 32 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 21 697 1140 2083 2565 Size: 21 676 443 943 482 Fragments arranged by size: 943 676 482 443 21 ZraI GAC'GTC Cuts at: 0 978 2565 Size: 978 1587 Enzymes that do cut: AatII AcuI AflII AflIII AleI AlwNI ApoI BamHI BanI BanII BbeI BciVI BclI BglI BglII BlpI Bme1580I BmrI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI BsmI Bsp1286I BspMI BsrBI BsrDI BsrGI BssSI BstAPI BstXI BstYI Bsu36I BtgI BtgZI BtsI DraIII EaeI EarI EciI Eco57MI EcoO109I HaeII Hin4I HincII KasI MfeI MmeI MscI MslI MspA1I NarI NcoI NspI PasI PciI PflMI PfoI PmlI PpiI PpuMI PstI PvuI PvuII SanDI SfcI SfoI SmlI StyI TaqII TatI TstI XcmI ZraI Enzymes that do not cut: AarI AccI Acc65I AclI AfeI AgeI AhdI AlfI AloI ApaI ApaLI AscI AseI AsiSI AvaI AvrII BaeI BbsI BbvCI BcgI BfrBI BmgBI BmtI BplI BsgI BsiWI BsmBI BspEI BspHI BsrFI BssHII BstBI BstEII BstZ17I ClaI CspCI DraI DrdI EagI EcoICRI EcoNI EcoRI EcoRV FalI FseI FspI FspAI HindIII HpaI KpnI MluI NaeI NdeI NgoMIV NheI NotI NruI NsiI PacI PmeI PshAI PsiI PspOMI PspXI PsrI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI StuI SwaI Tth111I XbaI XhoI XmaI XmnI