(Circular) (Six-base) MAPSORT of: pFN21AB6221.seq Check: 712 from: 1 to: 6715 pFN21AB6221 6715 bp With 182 enzymes: SgfI * March 7, 2011 13:30 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3738 3738 Size: 6715 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4525 278 Size: 53 83 186 936 2989 2468 Fragments arranged by size: 2989 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4058 1937 Size: 2121 4594 Acc65I G'GTAC_C Cuts at: 2821 4036 2821 Size: 1215 5500 AclI AA'CG_TT Cuts at: 1563 4842 5215 1563 Size: 3279 373 3063 Fragments arranged by size: 3279 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2418 2468 2817 3068 3089 3390 3518 4778 Size: 786 50 349 251 21 301 128 1260 Cuts at: 4778 6162 6430 1632 Size: 1384 268 1917 Fragments arranged by size: 1917 1384 1260 786 349 301 268 251 128 50 21 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6518 AflIII A'CryG_T Cuts at: 1203 5614 6591 1203 Size: 4411 977 1327 Fragments arranged by size: 4411 1327 977 AgeI A'CCGG_T Cuts at: 5546 5546 Size: 6715 AhdI GACnn_n'nnGTC Cuts at: 5444 5444 Size: 6715 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6715 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6715 AlwNI CAG_nnn'CTG Cuts at: 2126 4458 6030 2126 Size: 2332 1572 2811 Fragments arranged by size: 2811 2332 1572 ApaLI G'TGCA_C Cuts at: 4772 5928 4772 Size: 1156 5559 ApoI r'AATT_y Cuts at: 1493 2514 2524 3559 4024 4250 4402 6511 1493 Size: 1021 10 1035 465 226 152 2109 1697 Fragments arranged by size: 2109 1697 1035 1021 465 226 152 10 AseI AT'TA_AT Cuts at: 160 1032 5269 160 Size: 872 4237 1606 Fragments arranged by size: 4237 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6715 AvaI C'yCGr_G Cuts at: 1870 1943 2053 4040 1870 Size: 73 110 1987 4545 Fragments arranged by size: 4545 1987 110 73 AvrII C'CTAG_G Cuts at: 2474 2474 Size: 6715 BamHI G'GATC_C Cuts at: 3876 4045 4412 3876 Size: 169 367 6179 Fragments arranged by size: 6179 367 169 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2030 2203 2821 3252 4036 Size: 325 217 627 243 173 618 431 784 Cuts at: 4036 5491 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 784 627 618 431 325 243 217 173 BanII G_rGCy'C Cuts at: 1391 3236 3557 4034 1391 Size: 1845 321 477 4072 Fragments arranged by size: 4072 1845 477 321 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2367 928 Size: 971 468 5276 Fragments arranged by size: 5276 971 468 BbvCI CC'TCA_GC Cuts at: 2274 2274 Size: 6715 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4906 4940 1903 Size: 34 2969 34 3678 Fragments arranged by size: 3678 2969 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4607 5817 4607 Size: 1210 5505 BclI T'GATC_A Cuts at: 1548 3328 3816 1548 Size: 1780 488 4447 Fragments arranged by size: 4447 1780 488 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3974 5326 136 Size: 107 122 71 631 605 2302 1352 1525 Fragments arranged by size: 2302 1525 1352 631 605 122 107 71 BglII A'GATC_T Cuts at: 6710 6710 Size: 6715 BlpI GC'TnA_GC Cuts at: 2760 4132 2760 Size: 1372 5343 Bme1580I G_kGCm'C Cuts at: 1790 4776 5932 1790 Size: 2986 1156 2573 Fragments arranged by size: 2986 2573 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6715 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2840 4446 5404 Size: 416 53 83 399 24 1423 1606 958 Cuts at: 5404 442 Size: 1753 Fragments arranged by size: 1753 1606 1423 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6715 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2271 2575 2763 5375 1239 Size: 1032 304 188 2612 2579 Fragments arranged by size: 2612 2579 1032 304 188 Bpu10I CC'TnA_GC Cuts at: 2274 2924 2274 Size: 650 6065 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2281 4840 5705 6003 2281 Size: 2559 865 298 2993 Fragments arranged by size: 2993 2559 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5378 882 Size: 617 3879 2219 Fragments arranged by size: 3879 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2809 4506 493 Size: 711 1605 1697 2702 Fragments arranged by size: 2702 1697 1605 711 BsaBI GATnn'nnATC Cuts at: 6709 6709 Size: 6715 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4522 4904 5539 275 Size: 53 83 186 936 2989 382 635 1451 Fragments arranged by size: 2989 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5148 5546 5820 5967 6378 5148 Size: 398 274 147 411 5485 Fragments arranged by size: 5485 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6490 Fragments arranged by size: 6490 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2284 2307 2375 1186 Size: 1098 23 68 5526 Fragments arranged by size: 5526 1098 68 23 BseYI C'CCAG_C Cuts at: 2018 2780 5918 6414 2018 Size: 762 3138 496 2319 Fragments arranged by size: 3138 2319 762 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2664 1163 Size: 573 928 5214 Fragments arranged by size: 5214 928 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4165 4926 5954 1540 Size: 275 186 2164 761 1028 2301 Fragments arranged by size: 2301 2164 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3544 4776 4861 5932 3544 Size: 1232 85 1071 4327 Fragments arranged by size: 4327 1232 1071 85 BsmI GAATG_Cn' Cuts at: 4226 4319 4226 Size: 93 6622 BsmBI CGTCTCn'nnnn_ Cuts at: 2150 2319 2150 Size: 169 6546 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3236 3544 3557 4034 4776 4861 5932 Size: 399 1446 308 13 477 742 85 1071 Cuts at: 5932 1391 Size: 2174 Fragments arranged by size: 2174 1446 1071 742 477 399 308 85 13 BspEI T'CCGG_A Cuts at: 6378 6378 Size: 6715 BspHI T'CATG_A Cuts at: 3445 4604 6334 3445 Size: 1159 1730 3826 Fragments arranged by size: 3826 1730 1159 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2686 3738 4070 844 Size: 1842 1052 332 3489 Fragments arranged by size: 3489 1842 1052 332 BsrBI CCG'CTC Cuts at: 1394 1823 4602 1394 Size: 429 2779 3507 Fragments arranged by size: 3507 2779 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3377 5210 5384 65 Size: 1183 2129 1833 174 1396 Fragments arranged by size: 2129 1833 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2014 2027 5359 5546 1700 Size: 110 204 13 3332 187 2869 Fragments arranged by size: 3332 2869 204 187 110 13 BsrGI T'GTAC_A Cuts at: 96 2246 96 Size: 2150 4565 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6715 BssSI C'ACGA_G Cuts at: 4775 5787 4775 Size: 1012 5703 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6715 BstBI TT'CG_AA Cuts at: 3718 5519 3718 Size: 1801 4914 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6715 BstYI r'GATC_y Cuts at: 1972 3876 4045 4412 4797 4814 6266 6352 6364 Size: 1904 169 367 385 17 1452 86 12 Cuts at: 6364 6710 1972 Size: 346 1977 Fragments arranged by size: 1977 1904 1452 385 367 346 169 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2358 2358 Size: 6715 BtgI C'CryG_G Cuts at: 513 1065 2004 2034 513 Size: 552 939 30 5194 Fragments arranged by size: 5194 939 552 30 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5976 Fragments arranged by size: 5976 683 56 BtsI GCAGTG_nn' Cuts at: 4231 5025 5045 4231 Size: 794 20 5901 Fragments arranged by size: 5901 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6715 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6680 DraI TTT'AAA Cuts at: 4019 4019 Size: 6715 DraIII CAC_nnn'GTG Cuts at: 2812 2812 Size: 6715 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5722 809 Size: 507 4406 1802 Fragments arranged by size: 4406 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 4162 5051 6680 Size: 54 1425 50 275 18 2332 889 1629 Cuts at: 6680 8 Size: 43 Fragments arranged by size: 2332 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4162 1537 Size: 275 2350 4090 Fragments arranged by size: 4090 2350 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2437 3127 4645 1353 Size: 1084 690 1518 3423 Fragments arranged by size: 3423 1518 1084 690 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5302 5676 5822 387 Size: 1064 138 3713 374 146 1280 Fragments arranged by size: 3713 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2271 2418 2468 2575 2763 2817 3068 Size: 393 639 147 50 107 188 54 251 Cuts at: 3068 3089 3390 3518 4778 5375 6162 6430 1239 Size: 21 301 128 1260 597 787 268 1524 Fragments arranged by size: 1524 1260 787 639 597 393 301 268 251 188 147 128 107 54 50 21 EcoRI G'AATT_C Cuts at: 2524 4024 4402 2524 Size: 1500 378 4837 Fragments arranged by size: 4837 1500 378 FspI TGC'GCA Cuts at: 5221 5221 Size: 6715 HaeII r_GCGC'y Cuts at: 1678 5862 1678 Size: 4184 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2935 2967 3496 3528 3841 Size: 32 135 32 1363 32 529 32 313 Cuts at: 3841 3873 5356 5388 5430 5462 1373 Size: 32 1483 32 42 32 2626 Fragments arranged by size: 2626 1483 1363 529 313 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4059 4305 669 Size: 988 281 2121 246 3079 Fragments arranged by size: 3079 2121 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6715 HpaI GTT'AAC Cuts at: 4305 4305 Size: 6715 KpnI G_GTAC'C Cuts at: 2825 4040 2825 Size: 1215 5500 MfeI C'AATT_G Cuts at: 2138 3780 4314 2138 Size: 1642 534 4539 Fragments arranged by size: 4539 1642 534 MluI A'CGCG_T Cuts at: 6591 6591 Size: 6715 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2346 3177 5829 6013 1305 Size: 1041 831 2652 184 2007 Fragments arranged by size: 2652 2007 1041 831 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4893 Fragments arranged by size: 4893 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3471 4673 5032 5191 518 Size: 648 2305 1202 359 159 2042 Fragments arranged by size: 2305 2042 1202 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2438 3079 3568 4131 4808 5956 6201 1606 Size: 832 641 489 563 677 1148 245 2120 Fragments arranged by size: 2120 1148 832 677 641 563 489 245 NaeI GCC'GGC Cuts at: 2016 2029 2016 Size: 13 6702 NcoI C'CATG_G Cuts at: 513 1065 2004 2034 513 Size: 552 939 30 5194 Fragments arranged by size: 5194 939 552 30 NdeI CA'TA_TG Cuts at: 387 387 Size: 6715 NgoMIV G'CCGG_C Cuts at: 2014 2027 2014 Size: 13 6702 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6715 NotI GC'GGCC_GC Cuts at: 4162 4162 Size: 6715 NspI r_CATG'y Cuts at: 1132 1229 2354 3196 4073 5618 1132 Size: 97 1125 842 877 1545 2229 Fragments arranged by size: 2229 1545 1125 877 842 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6715 PciI A'CATG_T Cuts at: 5614 5614 Size: 6715 PflMI CCAn_nnn'nTGG Cuts at: 1108 2852 1108 Size: 1744 4971 PfoI T'CCnGG_A Cuts at: 3209 3209 Size: 6715 PmeI GTTT'AAAC Cuts at: 4019 4019 Size: 6715 PmlI CAC'GTG Cuts at: 2809 2809 Size: 6715 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3447 3479 4784 4816 6297 6329 3447 Size: 32 1305 32 1481 32 3833 Fragments arranged by size: 3833 1481 1305 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6715 PsiI TTA'TAA Cuts at: 3167 4285 3167 Size: 1118 5597 PstI C_TGCA'G Cuts at: 830 4067 830 Size: 3237 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6715 PvuII CAG'CTG Cuts at: 3079 3568 3079 Size: 489 6226 SalI G'TCGA_C Cuts at: 1936 4057 1936 Size: 2121 4594 SbfI CC_TGCA'GG Cuts at: 4067 4067 Size: 6715 ScaI AGT'ACT Cuts at: 4963 6675 4963 Size: 1712 5003 SfcI C'TryA_G Cuts at: 826 1045 3319 4063 5198 5879 6070 826 Size: 219 2274 744 1135 681 191 1471 Fragments arranged by size: 2274 1471 1135 744 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6715 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6715 SmaI CCC'GGG Cuts at: 1872 2055 4042 1872 Size: 183 1987 4545 Fragments arranged by size: 4545 1987 183 SmlI C'TyrA_G Cuts at: 820 1017 1943 2296 4819 5720 5982 820 Size: 197 926 353 2523 901 262 1553 Fragments arranged by size: 2523 1553 926 901 353 262 197 SnaBI TAC'GTA Cuts at: 493 4506 493 Size: 4013 2702 SpeI A'CTAG_T Cuts at: 152 3596 152 Size: 3444 3271 SphI G_CATG'C Cuts at: 1132 4073 1132 Size: 2941 3774 SspI AAT'ATT Cuts at: 5 52 4639 5 Size: 47 4587 2081 Fragments arranged by size: 4587 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6715 StyI C'CwwG_G Cuts at: 513 1065 2004 2034 2207 2474 4154 513 Size: 552 939 30 173 267 1680 3074 Fragments arranged by size: 3074 1680 939 552 267 173 30 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2310 2587 2698 3664 3764 4742 4759 Size: 1255 730 277 111 966 100 978 17 Cuts at: 4759 4912 5097 5542 325 Size: 153 185 445 1498 Fragments arranged by size: 1498 1255 978 966 730 445 277 185 153 111 100 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2246 4961 6673 Size: 274 80 33 51 1443 269 2715 1712 Cuts at: 6673 96 Size: 138 Fragments arranged by size: 2715 1712 1443 274 269 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6675 6707 6675 Size: 32 6683 Tth111I GACn'n_nGTC Cuts at: 1316 3490 1316 Size: 2174 4541 XbaI T'CTAG_A Cuts at: 4051 4051 Size: 6715 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6715 XmaI C'CCGG_G Cuts at: 1870 2053 4040 1870 Size: 183 1987 4545 Fragments arranged by size: 4545 1987 183 XmnI GAAnn'nnTTC Cuts at: 2860 4844 2860 Size: 1984 4731 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4523 276 Size: 53 83 186 936 2989 2468 Fragments arranged by size: 2989 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI BaeI BbeI BfrBI BplI BsiWI BstXI BstZ17I EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NarI NruI NsiI PacI PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI