Restriction map for
pFN21AB7608
##################################
# NO CUT ENZYMES ( 122 enzymes) #
##################################
AatII AccI AccII AclI AcsI
AflII AflIII AgeI AhdI Alw44I
AocI Aor51HI ApaLI ApoI AscI
Asp700I AspEI AspI AviII AvrII
BamHI BbrPI BcgI BciVI BfrI
BlnI BsaAI BsaBI BsaI BsiEI
BsiWI BsmBI BsmI Bsp1407I BspDI
BspHI BspLU11I BsrBI BsrFI BsrGI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstUI BstXI BstZ17I
Bsu36I Cfr10I ClaI CpoI DraI
DraIII EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoO65I EcoRI EcoRV
EcoT22I FseI FspI HgaI HincII
HindII HindIII HpaI KspI MaeII
MamI MluI MvnI NaeI NdeI
NgoMIV NheI NotI NruI NsiI
NspI NspV PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PshAI Psp1406I PvuI RcaI RsrII
SacII SalI ScaI SexAI SfiI
SfuI SgrAI SnaBI SpeI SphI
SspBI SspI SwaI TaqI TfiI
Tth111I TthHB8I Van91I XbaI XcmI
XhoI XmnI
###################################
# ONE CUT ENZYMES ( 51 enzymes) #
###################################
Acc65I 1007 GGTACC G^GTACC
AcyI 179 GGCGCC G[GA]^CG[CT]C
AlwNI 1027 CAGGGCCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 356 GGGCCC GGGCC^C
AseI 991 ATTAAT AT^TAAT
AsnI 991 ATTAAT AT^TAAT
Asp718I 1007 GGTACC G^GTACC
AvaI 935 CCCGGG C^[CT]CG[GA]G
BalI 2 TGGCCA TGG^CCA
BbeI 179 GGCGCC GGCGC^C
BbvCI 21 GCTGAGG CCTCAGC(-5/-2)
BclI 870 TGATCA T^GATCA
BfaI 964 CTAG C^TAG
BglI 368 GCCACATGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 976 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 976 GCTGAGC GC^T[ACGT]AGC
BsaHI 179 GGCGCC G[GA]^CG[CT]C
BseRI 883 GAGGAG GAGGAG(10/8)
BsgI 397 GTGCAG GTGCAG(16/14)
BsmAI 632 GTCTC GTCTC(1/5)
BsoBI 935 CCCGGG C^[CT]CG[GA]G
BsrDI 263 CATTGC GCAATG(2/0)
BsrI 5 CCAGT ACTGG(1/-1)
CelII 976 GCTGAGC GC^T[ACGT]AGC
DrdI 532 GACCTGCCCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 2 TGGCCA [CT]^GGCC[GA]
EcoNI 576 CCTTCTCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FbaI 870 TGATCA T^GATCA
HaeII 179 GGCGCC [GA]GCGC^[CT]
Hin1I 179 GGCGCC G[GA]^CG[CT]C
HphI 432 TCACC GGTGA(8/7)
KasI 179 GGCGCC G^GCGCC
KpnI 1007 GGTACC GGTAC^C
MaeI 964 CTAG C^TAG
MfeI 674 CAATTG C^AATTG
MluNI 2 TGGCCA TGG^CCA
MscI 2 TGGCCA TGG^CCA
MunI 674 CAATTG C^AATTG
NarI 179 GGCGCC GG^CGCC
PpuMI 530 AGGACCT [GA]G^G[AT]CC[CT]
PshBI 991 ATTAAT AT^TAAT
SacI 979 GAGCTC GAGCT^C
SapI 331 GAAGAGC GCTCTTC(1/4)
SbfI 194 CCTGCAGG CCTGCA^GG
SfoI 179 GGCGCC GGC^GCC
SmaI 935 CCCGGG CCC^GGG
SmlI 580 CTCAAG C^T[CT][GA]AG
Sse8387I 194 CCTGCAGG CCTGCA^GG
Tsp509I 675 AATT ^AATT
XmaCI 935 CCCGGG C^CCGGG
XmaI 935 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 33 enzymes) #
###################################
AccIII 341 TCCGGA T^CCGGA
983 TCCGGA
AlwI 339 GATCC GGATC(4/5)
939 GGATC
AspHI 790 GTGCTC G[AT]GC[AT]^C
979 GAGCTC
BbsI 562 GAAGAC GAAGAC(2/6)
541 GTCTTC
BcnI 935 CCCGG CC^[GC]GG
936 CCGGG
BglII 569 AGATCT A^GATCT
1061 AGATCT
BpuAI 562 GAAGAC GAAGAC(2/6)
541 GTCTTC
BsaWI 341 TCCGGA [AT]^CCGG[AT]
983 TCCGGA
BseAI 341 TCCGGA T^CCGGA
983 TCCGGA
BsiHKAI 790 GTGCTC G[AT]GC[AT]^C
979 GAGCTC
BsmFI 44 GGGAC GGGAC(10/14)
131 GTCCC
BspEI 341 TCCGGA T^CCGGA
983 TCCGGA
BstF5I 426 CATCC GGATG(2/0)
1023 CATCC
BstYI 569 AGATCT [GA]^GATC[CT]
1061 AGATCT
CfoI 73 GCGC GCG^C
180 GCGC
DsaI 747 CCATGG C^C[GA][CT]GG
803 CCATGG
EcoT14I 747 CCATGG C^C[AT][AT]GG
803 CCATGG
FokI 426 CATCC GGATG(9/13)
1023 CATCC
HhaI 73 GCGC GCG^C
180 GCGC
HinP1I 73 GCGC G^CGC
180 GCGC
HinfI 171 GACTC G^A[ACGT]TC
897 GACTC
MflI 569 AGATCT [GA]^GATC[CT]
1061 AGATCT
MroI 341 TCCGGA T^CCGGA
983 TCCGGA
MseI 68 TTAA T^TAA
992 TTAA
NciI 935 CCCGG CC^[GC]GG
936 CCGGG
NcoI 747 CCATGG C^CATGG
803 CCATGG
PleI 171 GACTC GAGTC(4/5)
897 GACTC
PvuII 590 CAGCTG CAG^CTG
874 CAGCTG
SfaNI 217 GATGC GCATC(5/9)
1044 GATGC
StyI 747 CCATGG C^C[AT][AT]GG
803 CCATGG
Tru9I 68 TTAA T^TAA
992 TTAA
Tsp45I 472 GTCAC ^GT[GC]AC
605 GTCAC
XhoII 569 AGATCT [GA]^GATC[CT]
1061 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 52 enzymes) #
#########################################
AciI AfaI AluI AvaII BanI
BanII BbvI BmyI BpmI BsaJI
BsiYI BslI Bsp1286I BspMI BssKI
BstNI Cac8I Cfr13I DdeI DpnII
DraII EarI EcoO109I EcoRII FauI
Fnu4HI HaeIII HapII HpaII ItaI
Ksp632I MaeIII MboI MboII MnlI
MslI MspA1I MspI MvaI MwoI
NdeII NlaIII NlaIV PstI RsaI
Sau3AI Sau96I ScrFI SfcI StuI
TseI TspRI