(Circular) (Six-base) MAPSORT of: pFN21AB7608.seq Check: 1049 from: 1 to: 5776 pFN21AB7608 5776 bp With 182 enzymes: SgfI * December 17, 2010 17:28 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2150 2150 Size: 5776 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3586 278 Size: 53 83 186 936 2050 2468 Fragments arranged by size: 2468 2050 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3119 1937 Size: 1182 4594 Acc65I G'GTAC_C Cuts at: 3012 3097 3012 Size: 85 5691 AclI AA'CG_TT Cuts at: 1563 3903 4276 1563 Size: 2340 373 3063 Fragments arranged by size: 3063 2340 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2092 3839 5223 5491 1632 Size: 460 1747 1384 268 1917 Fragments arranged by size: 1917 1747 1384 460 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5579 AflIII A'CryG_T Cuts at: 1203 4675 5652 1203 Size: 3472 977 1327 Fragments arranged by size: 3472 1327 977 AgeI A'CCGG_T Cuts at: 4607 4607 Size: 5776 AhdI GACnn_n'nnGTC Cuts at: 4505 4505 Size: 5776 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5776 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5776 AlwNI CAG_nnn'CTG Cuts at: 3037 3519 5091 3037 Size: 482 1572 3722 Fragments arranged by size: 3722 1572 482 ApaI G_GGCC'C Cuts at: 2365 2365 Size: 5776 ApaLI G'TGCA_C Cuts at: 3833 4989 3833 Size: 1156 4620 ApoI r'AATT_y Cuts at: 1493 3085 3311 3463 5572 1493 Size: 1592 226 152 2109 1697 Fragments arranged by size: 2109 1697 1592 226 152 AseI AT'TA_AT Cuts at: 160 1032 2997 4330 160 Size: 872 1965 1333 1606 Fragments arranged by size: 1965 1606 1333 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5776 AvaI C'yCGr_G Cuts at: 1870 1943 2940 3101 1870 Size: 73 997 161 4545 Fragments arranged by size: 4545 997 161 73 BamHI G'GATC_C Cuts at: 3106 3473 3106 Size: 367 5409 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2184 2381 3012 3097 4552 Size: 325 217 627 397 197 631 85 1455 Cuts at: 4552 618 Size: 1842 Fragments arranged by size: 1842 1455 631 627 397 325 217 197 85 BanII G_rGCy'C Cuts at: 1391 2365 2695 2988 3095 1391 Size: 974 330 293 107 4072 Fragments arranged by size: 4072 974 330 293 107 BbeI G_GCGC'C Cuts at: 2188 2188 Size: 5776 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2539 2574 928 Size: 971 640 35 4130 Fragments arranged by size: 4130 971 640 35 BbvCI CC'TCA_GC Cuts at: 2027 2027 Size: 5776 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3967 4001 1903 Size: 34 2030 34 3678 Fragments arranged by size: 3678 2030 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3668 4878 3668 Size: 1210 4566 BclI T'GATC_A Cuts at: 1548 2875 1548 Size: 1327 4449 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2379 4387 136 Size: 107 122 71 631 605 707 2008 1525 Fragments arranged by size: 2008 1525 707 631 605 122 107 71 BglII A'GATC_T Cuts at: 2574 3066 5771 2574 Size: 492 2705 2579 Fragments arranged by size: 2705 2579 492 BlpI GC'TnA_GC Cuts at: 2982 3193 2982 Size: 211 5565 Bme1580I G_kGCm'C Cuts at: 1790 2302 2365 2384 3837 4993 1790 Size: 512 63 19 1453 1156 2573 Fragments arranged by size: 2573 1453 1156 512 63 19 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5776 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3507 4465 442 Size: 416 53 83 399 24 2090 958 1753 Fragments arranged by size: 2090 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5776 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2400 2871 3044 4436 1239 Size: 1161 471 173 1392 2579 Fragments arranged by size: 2579 1392 1161 471 173 Bpu10I CC'TnA_GC Cuts at: 2027 2027 Size: 5776 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2570 3901 4766 5064 2570 Size: 1331 865 298 3282 Fragments arranged by size: 3282 1331 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4439 882 Size: 617 2940 2219 Fragments arranged by size: 2940 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3567 493 Size: 711 2363 2702 Fragments arranged by size: 2702 2363 711 BsaBI GATnn'nnATC Cuts at: 5770 5770 Size: 5776 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2185 3583 3965 4600 Size: 53 83 186 936 652 1398 382 635 Cuts at: 4600 275 Size: 1451 Fragments arranged by size: 1451 1398 936 652 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2346 2988 4209 4607 4881 5028 5439 2346 Size: 642 1221 398 274 147 411 2683 Fragments arranged by size: 2683 1221 642 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2561 2583 2591 2613 2682 Size: 30 165 30 1158 22 8 22 69 Cuts at: 2682 2712 2760 2790 1178 Size: 30 48 30 4164 Fragments arranged by size: 4164 1158 165 69 48 30 30 30 30 22 22 8 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2903 1186 Size: 1717 4059 BseYI C'CCAG_C Cuts at: 2194 2289 4979 5475 2194 Size: 95 2690 496 2495 Fragments arranged by size: 2690 2495 496 95 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2423 1163 Size: 573 687 4516 Fragments arranged by size: 4516 687 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3226 3987 5015 1540 Size: 275 186 1225 761 1028 2301 Fragments arranged by size: 2301 1225 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2799 2988 3837 3922 4993 2799 Size: 189 849 85 1071 3582 Fragments arranged by size: 3582 1071 849 189 85 BsmI GAATG_Cn' Cuts at: 3287 3380 3287 Size: 93 5683 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2302 2365 2384 2695 2799 2988 3095 Size: 399 512 63 19 311 104 189 107 Cuts at: 3095 3837 3922 4993 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 512 399 311 189 107 104 85 63 19 BspEI T'CCGG_A Cuts at: 2346 2988 5439 2346 Size: 642 2451 2683 Fragments arranged by size: 2683 2451 642 BspHI T'CATG_A Cuts at: 3665 5395 3665 Size: 1730 4046 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2150 2193 2547 3131 844 Size: 1306 43 354 584 3489 Fragments arranged by size: 3489 1306 584 354 43 BsrBI CCG'CTC Cuts at: 1394 1823 3663 1394 Size: 429 1840 3507 Fragments arranged by size: 3507 1840 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2267 4271 4445 65 Size: 1183 1019 2004 174 1396 Fragments arranged by size: 2004 1396 1183 1019 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4420 4607 1700 Size: 110 2610 187 2869 Fragments arranged by size: 2869 2610 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5776 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5776 BssSI C'ACGA_G Cuts at: 3836 4848 3836 Size: 1012 4764 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5776 BstBI TT'CG_AA Cuts at: 4580 4580 Size: 5776 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5776 BstYI r'GATC_y Cuts at: 1972 2574 3066 3106 3473 3858 3875 5327 5413 Size: 602 492 40 367 385 17 1452 86 Cuts at: 5413 5425 5771 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 602 492 385 367 346 86 40 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2752 2808 513 Size: 552 939 748 56 3481 Fragments arranged by size: 3481 939 748 552 56 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5037 Fragments arranged by size: 5037 683 56 BtsI GCAGTG_nn' Cuts at: 2188 3292 4086 4106 2188 Size: 1104 794 20 3858 Fragments arranged by size: 3858 1104 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5776 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5741 DraI TTT'AAA Cuts at: 3080 3080 Size: 5776 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2543 4783 809 Size: 507 1227 2240 1802 Fragments arranged by size: 2240 1802 1227 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2007 3223 4112 Size: 54 1425 50 275 18 177 1216 889 Cuts at: 4112 5741 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1216 889 275 177 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3223 1537 Size: 275 1411 4090 Fragments arranged by size: 4090 1411 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2331 2673 2822 3706 1353 Size: 978 342 149 884 3423 Fragments arranged by size: 3423 978 884 342 149 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4363 4737 4883 387 Size: 1064 138 2774 374 146 1280 Fragments arranged by size: 2774 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2986 2986 Size: 5776 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2092 2400 2871 3044 3839 4436 5223 Size: 393 460 308 471 173 795 597 787 Cuts at: 5223 5491 1239 Size: 268 1524 Fragments arranged by size: 1524 795 787 597 471 460 393 308 268 173 EcoNI CCTnn'n_nnAGG Cuts at: 2585 2585 Size: 5776 EcoO109I rG'GnC_Cy Cuts at: 2361 2536 2937 3034 2361 Size: 175 401 97 5103 Fragments arranged by size: 5103 401 175 97 EcoRI G'AATT_C Cuts at: 3085 3463 3085 Size: 378 5398 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2122 2122 Size: 5776 FspI TGC'GCA Cuts at: 4282 4282 Size: 5776 HaeII r_GCGC'y Cuts at: 1678 2188 4923 1678 Size: 510 2735 2531 Fragments arranged by size: 2735 2531 510 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2561 2593 4417 4449 4491 Size: 32 135 32 989 32 1824 32 42 Cuts at: 4491 4523 1373 Size: 32 2626 Fragments arranged by size: 2626 1824 989 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3120 3366 669 Size: 988 281 1182 246 3079 Fragments arranged by size: 3079 1182 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5776 HpaI GTT'AAC Cuts at: 3366 3366 Size: 5776 KasI G'GCGC_C Cuts at: 2184 2184 Size: 5776 KpnI G_GTAC'C Cuts at: 3016 3101 3016 Size: 85 5691 MfeI C'AATT_G Cuts at: 2679 3375 2679 Size: 696 5080 MluI A'CGCG_T Cuts at: 5652 5652 Size: 5776 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4890 5074 1305 Size: 3585 184 2007 Fragments arranged by size: 3585 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2009 10 Size: 54 1425 343 177 3777 Fragments arranged by size: 3777 1425 343 177 54 MslI CAynn'nnrTG Cuts at: 518 1166 2073 2504 2751 3734 4093 4252 518 Size: 648 907 431 247 983 359 159 2042 Fragments arranged by size: 2042 983 907 648 431 359 247 159 MspA1I CmG'CkG Cuts at: 1606 2293 2597 2881 3055 3192 3869 5017 5262 Size: 687 304 284 174 137 677 1148 245 Cuts at: 5262 1606 Size: 2120 Fragments arranged by size: 2120 1148 687 677 304 284 245 174 137 NarI GG'CG_CC Cuts at: 2185 2185 Size: 5776 NcoI C'CATG_G Cuts at: 513 1065 2004 2752 2808 513 Size: 552 939 748 56 3481 Fragments arranged by size: 3481 939 748 552 56 NdeI CA'TA_TG Cuts at: 387 387 Size: 5776 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5776 NotI GC'GGCC_GC Cuts at: 3223 3223 Size: 5776 NspI r_CATG'y Cuts at: 1132 1229 3134 4679 1132 Size: 97 1905 1545 2229 Fragments arranged by size: 2229 1905 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5776 PciI A'CATG_T Cuts at: 4675 4675 Size: 5776 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5776 PfoI T'CCnGG_A Cuts at: 3060 3060 Size: 5776 PmeI GTTT'AAAC Cuts at: 3080 3080 Size: 5776 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3845 3877 5358 5390 3845 Size: 32 1481 32 4231 Fragments arranged by size: 4231 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2536 2536 Size: 5776 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5776 PsiI TTA'TAA Cuts at: 3346 3346 Size: 5776 PspOMI G'GGCC_C Cuts at: 2361 2361 Size: 5776 PstI C_TGCA'G Cuts at: 830 2204 2775 3074 3128 830 Size: 1374 571 299 54 3478 Fragments arranged by size: 3478 1374 571 299 54 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5776 PvuII CAG'CTG Cuts at: 2597 2881 2597 Size: 284 5492 SacI G_AGCT'C Cuts at: 2988 2988 Size: 5776 SalI G'TCGA_C Cuts at: 1936 3118 1936 Size: 1182 4594 SapI GCTCTTCn'nnn_ Cuts at: 2331 2331 Size: 5776 SbfI CC_TGCA'GG Cuts at: 2204 3128 2204 Size: 924 4852 ScaI AGT'ACT Cuts at: 4024 5736 4024 Size: 1712 4064 SfcI C'TryA_G Cuts at: 826 1045 2200 2771 3070 3124 4259 4940 5131 Size: 219 1155 571 299 54 1135 681 191 Cuts at: 5131 826 Size: 1471 Fragments arranged by size: 1471 1155 1135 681 571 299 219 191 54 SfoI GGC'GCC Cuts at: 2186 2186 Size: 5776 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5776 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5776 SmaI CCC'GGG Cuts at: 1872 2942 3103 1872 Size: 1070 161 4545 Fragments arranged by size: 4545 1070 161 SmlI C'TyrA_G Cuts at: 820 1017 1943 2585 3880 4781 5043 820 Size: 197 926 642 1295 901 262 1553 Fragments arranged by size: 1553 1295 926 901 642 262 197 SnaBI TAC'GTA Cuts at: 493 3567 493 Size: 3074 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5776 SphI G_CATG'C Cuts at: 1132 3134 1132 Size: 2002 3774 SspI AAT'ATT Cuts at: 5 52 3700 5 Size: 47 3648 2081 Fragments arranged by size: 3648 2081 47 StuI AGG'CCT Cuts at: 1517 2413 2973 3005 1517 Size: 896 560 32 4288 Fragments arranged by size: 4288 896 560 32 StyI C'CwwG_G Cuts at: 513 1065 2004 2752 2808 3215 513 Size: 552 939 748 56 407 3074 Fragments arranged by size: 3074 939 748 552 407 56 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2859 3803 3820 3973 4158 4603 325 Size: 1255 1279 944 17 153 185 445 1498 Fragments arranged by size: 1498 1279 1255 944 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4022 5734 96 Size: 274 80 33 51 1443 2045 1712 138 Fragments arranged by size: 2045 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2583 2615 2637 2669 5736 5768 2583 Size: 32 22 32 3067 32 2591 Fragments arranged by size: 3067 2591 32 32 32 22 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5776 XbaI T'CTAG_A Cuts at: 3112 3112 Size: 5776 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5776 XmaI C'CCGG_G Cuts at: 1870 2940 3101 1870 Size: 1070 161 4545 Fragments arranged by size: 4545 1070 161 XmnI GAAnn'nnTTC Cuts at: 3905 3905 Size: 5776 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3584 276 Size: 53 83 186 936 2050 2468 Fragments arranged by size: 2468 2050 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoRV FseI FspAI NaeI NgoMIV NruI NsiI PacI PmlI PspXI PsrI RsrII SacII SanDI SexAI SfiI SrfI SwaI XcmI