################################## # NO CUT ENZYMES ( 176 enzymes) # ################################## AatII Acc65I AccII AccIII AclI AcsI AcyI AflII AflIII AgeI AhdI Alw44I AlwI AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AvaI AvaII AviII AvrII BamHI BanII BbeI BbrPI BbsI BbvCI BcgI BclI BcnI BfrI BglI BglII BlnI BlpI Bpu1102I BpuAI BsaAI BsaHI BsaI BsaWI BseAI BseRI BsiEI BsiHKAI BsiWI BsmBI BsmFI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrFI BsrGI BsrI BssHII BssSI BstBI BstUI BstYI Bsu36I CelII CfoI Cfr10I ClaI CpoI DraI DraIII DsaI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoRI EcoRV EcoT22I FauI FbaI FseI FspI HaeII HgaI HhaI Hin1I HinP1I HincII HindII HindIII HinfI HpaI KasI KpnI Ksp632I KspI MaeII MfeI MflI MluI MroI MunI MvnI NaeI NarI NciI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PleI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI RcaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI Sse8387I SspBI SspI StuI SwaI TaqI TfiI Tth111I TthHB8I Van91I XbaI XcmI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 39 enzymes) # ################################### AccI 895 GTATAC GT^[AC][GT]AC AlwNI 239 CAGAGACTG CAG[ACGT][ACGT][ACGT]^CTG BalI 404 TGGCCA TGG^CCA BanI 549 GGTGCC G^G[CT][GA]CC BciVI 455 GTATCC GTATCC(6/5) BmyI 550 GTGCCC G[AGT]GC[ACT]^C BpmI 279 CTCCAG CTGGAG(16/14) BsaBI 187 GATCTTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsgI 933 GTGCAG GTGCAG(16/14) BsmAI 241 GAGAC GTCTC(1/5) BsmI 272 GAATGC GAATGC(1/-1) Bsp1286I 550 GTGCCC G[AGT]GC[ACT]^C BssKI 468 CCTGG ^CC[ACGT]GG Bst1107I 895 GTATAC GTA^TAC BstAPI 316 GCAGGGTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstNI 468 CCTGG CC^[AT]GG BstXI 305 CCATGTCATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstZ17I 895 GTATAC GTA^TAC DraII 472 GGGGCCT [GA]G^G[ACGT]CC[CT] DrdI 601 GACACCACTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EaeI 404 TGGCCA [CT]^GGCC[GA] EcoO109I 472 GGGGCCT [GA]G^G[ACGT]CC[CT] EcoRII 468 CCTGG ^CC[AT]GG EcoT14I 75 CCAAGG C^C[AT][AT]GG HapII 729 CCGG C^CGG HpaII 729 CCGG C^CGG MamI 187 GATCTTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MluNI 404 TGGCCA TGG^CCA MscI 404 TGGCCA TGG^CCA MseI 142 TTAA T^TAA MspI 729 CCGG C^CGG MvaI 468 CCTGG CC^[AT]GG NspI 224 GCATGC [GA]CATG^[CT] PstI 477 CTGCAG CTGCA^G ScrFI 468 CCTGG CC^[ACGT]GG SphI 224 GCATGC GCATG^C StyI 75 CCAAGG C^C[AT][AT]GG Tru9I 142 TTAA T^TAA Tsp509I 183 AATT ^AATT ################################### # TWO CUT ENZYMES ( 17 enzymes) # ################################### AciI 396 CCGC CCGC(-3/-1) 573 CCGC AfaI 210 GTAC GT^AC 655 GTAC BfaI 86 CTAG C^TAG 542 CTAG BsaJI 75 CCAAGG C^C[ACGT][ACGT]GG 468 CCTGGG BsrDI 798 CATTGC GCAATG(2/0) 382 GCAATG BstEII 527 GGTTACC G^GT[ACGT]ACC 855 GGTGACC BstPI 527 GGTTACC G^GT[ACGT]ACC 855 GGTGACC Cfr13I 473 GGGCC G^G[ACGT]CC 726 GGGCC EcoO65I 527 GGTTACC G^GT[ACGT]ACC 855 GGTGACC HphI 855 GGTGA GGTGA(8/7) 107 TCACC MaeI 86 CTAG C^TAG 542 CTAG MspA1I 786 CAGCTG C[AC]G^C[GT]G 844 CAGCTG PvuII 786 CAGCTG CAG^CTG 844 CAGCTG RsaI 210 GTAC GT^AC 655 GTAC Sau96I 473 GGGCC G^G[ACGT]CC 726 GGGCC SfcI 477 CTGCAG C^T[GA][CT]AG 783 CTACAG SmlI 706 CTCAAG C^T[CT][GA]AG 808 CTCAAG ######################################### # 3 OR MORE CUT ENZYMES ( 26 enzymes) # ######################################### AluI BbvI BsiYI BslI BstF5I Cac8I DdeI DpnII Fnu4HI FokI HaeIII ItaI MaeIII MboI MboII MnlI MslI MwoI NdeII NlaIII NlaIV Sau3AI SfaNI TseI Tsp45I TspRI