(Circular) (Six-base) MAPSORT of: pFN21AE2558.seq Check: 8087 from: 1 to: 7075 pFN21AE2558 7075 bp With 182 enzymes: SgfI * December 25, 2014 18:02 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2196 2196 Size: 7075 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4885 278 Size: 53 83 186 936 3349 2468 Fragments arranged by size: 3349 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4254 4356 4418 1937 Size: 2317 102 62 4594 Fragments arranged by size: 4594 2317 102 62 Acc65I G'GTAC_C Cuts at: 4170 4396 4170 Size: 226 6849 AclI AA'CG_TT Cuts at: 1563 5202 5575 1563 Size: 3639 373 3063 Fragments arranged by size: 3639 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2612 3460 3644 5138 6522 6790 1632 Size: 980 848 184 1494 1384 268 1917 Fragments arranged by size: 1917 1494 1384 980 848 268 184 AfeI AGC'GCT Cuts at: 2486 2486 Size: 7075 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6878 AflIII A'CryG_T Cuts at: 1203 4113 5974 6951 1203 Size: 2910 1861 977 1327 Fragments arranged by size: 2910 1861 1327 977 AgeI A'CCGG_T Cuts at: 5906 5906 Size: 7075 AhdI GACnn_n'nnGTC Cuts at: 3974 5804 3974 Size: 1830 5245 AleI CACnn'nnGTG Cuts at: 1166 2297 2882 1166 Size: 1131 585 5359 Fragments arranged by size: 5359 1131 585 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 4067 4216 1184 Size: 2883 149 4043 Fragments arranged by size: 4043 2883 149 AlwNI CAG_nnn'CTG Cuts at: 2239 2248 2431 2527 3868 3970 4818 6390 2239 Size: 9 183 96 1341 102 848 1572 2924 Fragments arranged by size: 2924 1572 1341 848 183 102 96 9 ApaI G_GGCC'C Cuts at: 2170 2550 2170 Size: 380 6695 ApaLI G'TGCA_C Cuts at: 3926 4316 5132 6288 3926 Size: 390 816 1156 4713 Fragments arranged by size: 4713 1156 816 390 ApoI r'AATT_y Cuts at: 1493 4384 4610 4762 6871 1493 Size: 2891 226 152 2109 1697 Fragments arranged by size: 2891 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 5629 160 Size: 872 4597 1606 Fragments arranged by size: 4597 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7075 AvaI C'yCGr_G Cuts at: 1870 1943 2140 2157 3164 4073 4400 1870 Size: 73 197 17 1007 909 327 4545 Fragments arranged by size: 4545 1007 909 327 197 73 17 BamHI G'GATC_C Cuts at: 2685 4405 4772 2685 Size: 1720 367 4988 Fragments arranged by size: 4988 1720 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2099 2323 2602 2646 2932 Size: 325 217 627 312 224 279 44 286 Cuts at: 2932 3542 4170 4345 4396 5851 618 Size: 610 628 175 51 1455 1842 Fragments arranged by size: 1842 1455 628 627 610 325 312 286 279 224 217 175 51 44 BanII G_rGCy'C Cuts at: 1391 2170 2217 2256 2550 3444 4290 4394 1391 Size: 779 47 39 294 894 846 104 4072 Fragments arranged by size: 4072 894 846 779 294 104 47 39 BbeI G_GCGC'C Cuts at: 2103 2327 3546 2103 Size: 224 1219 5632 Fragments arranged by size: 5632 1219 224 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3550 928 Size: 971 1651 4453 Fragments arranged by size: 4453 1651 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 5266 5300 1903 Size: 34 50 34 3245 34 3678 Fragments arranged by size: 3678 3245 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4967 6177 4967 Size: 1210 5865 BclI T'GATC_A Cuts at: 1548 1548 Size: 7075 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2133 5686 136 Size: 107 122 71 631 605 461 3553 1525 Fragments arranged by size: 3553 1525 631 605 461 122 107 71 BglII A'GATC_T Cuts at: 4143 7070 4143 Size: 2927 4148 BlpI GC'TnA_GC Cuts at: 3669 4492 3669 Size: 823 6252 Bme1580I G_kGCm'C Cuts at: 1790 2170 2470 2550 2586 2607 3930 4320 5136 Size: 380 300 80 36 21 1323 390 816 Cuts at: 5136 6292 1790 Size: 1156 2573 Fragments arranged by size: 2573 1323 1156 816 390 380 300 80 36 21 BmgBI CAC'GTC Cuts at: 1318 2635 2796 3064 4114 4217 1318 Size: 1317 161 268 1050 103 4176 Fragments arranged by size: 4176 1317 1050 268 161 103 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2513 4806 5764 Size: 416 53 83 399 24 1096 2293 958 Cuts at: 5764 442 Size: 1753 Fragments arranged by size: 2293 1753 1096 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 3882 1056 Size: 2826 4249 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2410 2410 Size: 7075 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2270 2405 2690 2720 3023 3219 3342 3825 Size: 1031 135 285 30 303 196 123 483 Cuts at: 3825 5735 1239 Size: 1910 2579 Fragments arranged by size: 2579 1910 1031 483 303 285 196 135 123 30 Bpu10I CC'TnA_GC Cuts at: 3588 3588 Size: 7075 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3506 5200 6065 6363 3506 Size: 1694 865 298 4218 Fragments arranged by size: 4218 1694 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2315 2836 2969 3014 3195 3244 5738 Size: 617 816 521 133 45 181 49 2494 Cuts at: 5738 882 Size: 2219 Fragments arranged by size: 2494 2219 816 617 521 181 133 49 45 BsaAI yAC'GTr Cuts at: 493 1204 4235 4866 493 Size: 711 3031 631 2702 Fragments arranged by size: 3031 2702 711 631 BsaBI GATnn'nnATC Cuts at: 7069 7069 Size: 7075 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2100 2324 3543 3632 Size: 53 83 186 936 567 224 1219 89 Cuts at: 3632 3717 4882 5264 5899 275 Size: 85 1165 382 635 1451 Fragments arranged by size: 1451 1219 1165 936 635 567 382 224 186 89 85 83 53 BsaWI w'CCGG_w Cuts at: 5508 5906 6180 6327 6738 5508 Size: 398 274 147 411 5845 Fragments arranged by size: 5845 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3192 3222 4163 4193 1178 Size: 30 165 30 1789 30 941 30 4060 Fragments arranged by size: 4060 1789 941 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2128 2337 2507 2510 1186 Size: 942 209 170 3 5751 Fragments arranged by size: 5751 942 209 170 3 BseYI C'CCAG_C Cuts at: 2046 2487 2799 3532 4290 6278 6774 2046 Size: 441 312 733 758 1988 496 2347 Fragments arranged by size: 2347 1988 758 733 496 441 312 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2429 2438 2858 2888 3706 1163 Size: 573 693 9 420 30 818 4532 Fragments arranged by size: 4532 818 693 573 420 30 9 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4525 5286 6314 1540 Size: 275 186 2524 761 1028 2301 Fragments arranged by size: 2524 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2217 2256 2970 3303 3522 3646 3930 4137 4320 Size: 39 714 333 219 124 284 207 183 Cuts at: 4320 5136 5221 6292 2217 Size: 816 85 1071 3000 Fragments arranged by size: 3000 1071 816 714 333 284 219 207 183 124 85 39 BsmI GAATG_Cn' Cuts at: 4586 4679 4586 Size: 93 6982 BsmBI CGTCTCn'nnnn_ Cuts at: 2854 2943 2854 Size: 89 6986 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2170 2217 2256 2470 2550 2586 2607 Size: 399 380 47 39 214 80 36 21 Cuts at: 2607 2970 3303 3444 3522 3646 3930 4137 4290 Size: 363 333 141 78 124 284 207 153 Cuts at: 4290 4320 4394 5136 5221 6292 1391 Size: 30 74 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 380 363 333 284 214 207 153 141 124 85 80 78 74 47 39 36 30 21 BspEI T'CCGG_A Cuts at: 6738 6738 Size: 7075 BspHI T'CATG_A Cuts at: 3390 4964 6694 3390 Size: 1574 1730 3771 Fragments arranged by size: 3771 1730 1574 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2196 4430 844 Size: 1352 2234 3489 Fragments arranged by size: 3489 2234 1352 BsrBI CCG'CTC Cuts at: 1394 1823 4228 4962 1394 Size: 429 2405 734 3507 Fragments arranged by size: 3507 2405 734 429 BsrDI GCAATG_nn' Cuts at: 65 1248 5570 5744 65 Size: 1183 4322 174 1396 Fragments arranged by size: 4322 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2678 2929 3007 5719 5906 1700 Size: 110 868 251 78 2712 187 2869 Fragments arranged by size: 2869 2712 868 251 187 110 78 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7075 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7075 BssSI C'ACGA_G Cuts at: 5135 6147 5135 Size: 1012 6063 BstAPI GCAn_nnn'nTGC Cuts at: 794 2239 3703 3925 794 Size: 1445 1464 222 3944 Fragments arranged by size: 3944 1464 1445 222 BstBI TT'CG_AA Cuts at: 5879 5879 Size: 7075 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7075 BstXI CCAn_nnnn'nTGG Cuts at: 3817 3817 Size: 7075 BstYI r'GATC_y Cuts at: 1972 2685 4143 4405 4772 5157 5174 6626 6712 Size: 713 1458 262 367 385 17 1452 86 Cuts at: 6712 6724 7070 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1458 1452 713 385 367 346 262 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2131 2712 3796 513 Size: 552 1066 581 1084 3792 Fragments arranged by size: 3792 1084 1066 581 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2164 487 Size: 683 56 938 5398 Fragments arranged by size: 5398 938 683 56 BtsI GCAGTG_nn' Cuts at: 2537 4591 5385 5405 2537 Size: 2054 794 20 4207 Fragments arranged by size: 4207 2054 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7075 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3292 3327 529 Size: 35 2728 35 4277 Fragments arranged by size: 4277 2728 35 35 DraI TTT'AAA Cuts at: 4379 4379 Size: 7075 DraIII CAC_nnn'GTG Cuts at: 3703 3703 Size: 7075 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6082 809 Size: 507 4766 1802 Fragments arranged by size: 4766 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2757 2859 3106 Size: 54 1425 50 275 18 927 102 247 Cuts at: 3106 3258 3372 3939 4229 4522 5411 7040 8 Size: 152 114 567 290 293 889 1629 43 Fragments arranged by size: 1629 1425 927 889 567 293 290 275 247 152 114 102 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4522 1537 Size: 275 2710 4090 Fragments arranged by size: 4090 2710 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2297 2957 3281 5005 1353 Size: 944 660 324 1724 3423 Fragments arranged by size: 3423 1724 944 660 324 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2031 2523 3106 5662 6036 6182 Size: 1064 138 442 492 583 2556 374 146 Cuts at: 6182 387 Size: 1280 Fragments arranged by size: 2556 1280 1064 583 492 442 374 146 138 EcoICRI GAG'CTC Cuts at: 2215 2254 2215 Size: 39 7036 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2270 2405 2612 2690 2720 3023 3219 Size: 393 638 135 207 78 30 303 196 Cuts at: 3219 3342 3460 3644 3825 5138 5735 6522 6790 Size: 123 118 184 181 1313 597 787 268 Cuts at: 6790 1239 Size: 1524 Fragments arranged by size: 1524 1313 787 638 597 393 303 268 207 196 184 181 135 123 118 78 30 EcoNI CCTnn'n_nnAGG Cuts at: 2672 4340 2672 Size: 1668 5407 EcoO109I rG'GnC_Cy Cuts at: 2166 2167 2269 2413 2546 2547 2665 3169 3772 Size: 1 102 144 133 1 118 504 603 Cuts at: 3772 3845 3934 2166 Size: 73 89 5307 Fragments arranged by size: 5307 603 504 144 133 118 102 89 73 1 1 EcoRI G'AATT_C Cuts at: 4384 4762 4384 Size: 378 6697 EcoRV GAT'ATC Cuts at: 4009 4009 Size: 7075 FspI TGC'GCA Cuts at: 5581 5581 Size: 7075 HaeII r_GCGC'y Cuts at: 1678 2103 2327 2488 3508 3546 6222 1678 Size: 425 224 161 1020 38 2676 2531 Fragments arranged by size: 2676 2531 1020 425 224 161 38 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3960 3992 5716 5748 5790 Size: 32 135 32 2388 32 1724 32 42 Cuts at: 5790 5822 1373 Size: 32 2626 Fragments arranged by size: 2626 2388 1724 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2845 3067 4419 4665 669 Size: 988 281 907 222 1352 246 3079 Fragments arranged by size: 3079 1352 988 907 281 246 222 HindIII A'AGCT_T Cuts at: 748 748 Size: 7075 HpaI GTT'AAC Cuts at: 4665 4665 Size: 7075 KasI G'GCGC_C Cuts at: 2099 2323 3542 2099 Size: 224 1219 5632 Fragments arranged by size: 5632 1219 224 KpnI G_GTAC'C Cuts at: 4174 4400 4174 Size: 226 6849 MfeI C'AATT_G Cuts at: 4674 4674 Size: 7075 MluI A'CGCG_T Cuts at: 6951 6951 Size: 7075 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4203 4206 6189 6373 1305 Size: 2898 3 1983 184 2007 Fragments arranged by size: 2898 2007 1983 184 3 MscI TGG'CCA Cuts at: 10 64 1489 1832 2759 3374 3941 10 Size: 54 1425 343 927 615 567 3144 Fragments arranged by size: 3144 1425 927 615 567 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2297 2717 2882 5033 5392 5551 518 Size: 648 1131 420 165 2151 359 159 2042 Fragments arranged by size: 2151 2042 1131 648 420 359 165 159 MspA1I CmG'CkG Cuts at: 1606 2046 2574 2872 3865 4282 4491 5168 6316 Size: 440 528 298 993 417 209 677 1148 Cuts at: 6316 6561 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 993 677 528 440 417 298 245 209 NaeI GCC'GGC Cuts at: 2680 2931 3009 2680 Size: 251 78 6746 Fragments arranged by size: 6746 251 78 NarI GG'CG_CC Cuts at: 2100 2324 3543 2100 Size: 224 1219 5632 Fragments arranged by size: 5632 1219 224 NcoI C'CATG_G Cuts at: 513 1065 3796 513 Size: 552 2731 3792 Fragments arranged by size: 3792 2731 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 7075 NgoMIV G'CCGG_C Cuts at: 2678 2929 3007 2678 Size: 251 78 6746 Fragments arranged by size: 6746 251 78 NheI G'CTAG_C Cuts at: 1052 3878 1052 Size: 2826 4249 NotI GC'GGCC_GC Cuts at: 4522 4522 Size: 7075 NspI r_CATG'y Cuts at: 1132 1229 2563 4433 5978 1132 Size: 97 1334 1870 1545 2229 Fragments arranged by size: 2229 1870 1545 1334 97 PasI CC'CwG_GG Cuts at: 1348 2743 3850 1348 Size: 1395 1107 4573 Fragments arranged by size: 4573 1395 1107 PciI A'CATG_T Cuts at: 5974 5974 Size: 7075 PflMI CCAn_nnn'nTGG Cuts at: 1108 3365 3970 4062 1108 Size: 2257 605 92 4121 Fragments arranged by size: 4121 2257 605 92 PfoI T'CCnGG_A Cuts at: 2396 3000 2396 Size: 604 6471 PmeI GTTT'AAAC Cuts at: 4379 4379 Size: 7075 PmlI CAC'GTG Cuts at: 4235 4235 Size: 7075 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5144 5176 6657 6689 5144 Size: 32 1481 32 5530 Fragments arranged by size: 5530 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3169 3772 3934 3169 Size: 603 162 6310 Fragments arranged by size: 6310 603 162 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7075 PsiI TTA'TAA Cuts at: 4645 4645 Size: 7075 PspOMI G'GGCC_C Cuts at: 2166 2546 2166 Size: 380 6695 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 3455 3487 4079 4111 3455 Size: 32 592 32 6419 Fragments arranged by size: 6419 592 32 32 PstI C_TGCA'G Cuts at: 830 2244 2532 3690 4427 830 Size: 1414 288 1158 737 3478 Fragments arranged by size: 3478 1414 1158 737 288 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7075 PvuII CAG'CTG Cuts at: 2046 2574 2872 3865 2046 Size: 528 298 993 5256 Fragments arranged by size: 5256 993 528 298 SacI G_AGCT'C Cuts at: 2217 2256 2217 Size: 39 7036 SalI G'TCGA_C Cuts at: 1936 4417 1936 Size: 2481 4594 SapI GCTCTTCn'nnn_ Cuts at: 2957 2957 Size: 7075 SbfI CC_TGCA'GG Cuts at: 4427 4427 Size: 7075 ScaI AGT'ACT Cuts at: 5323 7035 5323 Size: 1712 5363 SfcI C'TryA_G Cuts at: 826 1045 2240 2480 2528 3686 4012 4423 5558 Size: 219 1195 240 48 1158 326 411 1135 Cuts at: 5558 6239 6430 826 Size: 681 191 1471 Fragments arranged by size: 1471 1195 1158 1135 681 411 326 240 219 191 48 SfoI GGC'GCC Cuts at: 2101 2325 3544 2101 Size: 224 1219 5632 Fragments arranged by size: 5632 1219 224 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7075 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7075 SmaI CCC'GGG Cuts at: 1872 2159 4402 1872 Size: 287 2243 4545 Fragments arranged by size: 4545 2243 287 SmlI C'TyrA_G Cuts at: 820 1017 1943 3521 5179 6080 6342 820 Size: 197 926 1578 1658 901 262 1553 Fragments arranged by size: 1658 1578 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4866 493 Size: 4373 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7075 SphI G_CATG'C Cuts at: 1132 4433 1132 Size: 3301 3774 SspI AAT'ATT Cuts at: 5 52 4999 5 Size: 47 4947 2081 Fragments arranged by size: 4947 2081 47 StuI AGG'CCT Cuts at: 1517 3113 1517 Size: 1596 5479 StyI C'CwwG_G Cuts at: 513 1065 3445 3796 4514 513 Size: 552 2380 351 718 3074 Fragments arranged by size: 3074 2380 718 552 351 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3074 3389 5102 5119 5272 5457 5902 Size: 1255 1494 315 1713 17 153 185 445 Cuts at: 5902 325 Size: 1498 Fragments arranged by size: 1713 1498 1494 1255 445 315 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 5321 7033 96 Size: 274 80 33 51 1443 3344 1712 138 Fragments arranged by size: 3344 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3192 3224 7035 7067 3192 Size: 32 3811 32 3200 Fragments arranged by size: 3811 3200 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7075 XbaI T'CTAG_A Cuts at: 4411 4411 Size: 7075 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2614 2614 Size: 7075 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7075 XmaI C'CCGG_G Cuts at: 1870 2157 4400 1870 Size: 287 2243 4545 Fragments arranged by size: 4545 2243 287 XmnI GAAnn'nnTTC Cuts at: 3557 5204 3557 Size: 1647 5428 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4883 276 Size: 53 83 186 936 3349 2468 Fragments arranged by size: 3349 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PsrI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI AvrII BaeI BbvCI BfrBI BsiWI BstZ17I Bsu36I FalI FseI FspAI NruI NsiI PacI PspXI RsrII SacII SanDI SexAI SfiI SrfI SwaI