################################## # NO CUT ENZYMES ( 185 enzymes) # ################################## AatII Acc65I AccI AccII AclI AcsI AcyI AfaI AflII AflIII AgeI AhdI Alw44I AlwNI AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvaI AvaII AviII AvrII BanI BbeI BbrPI BbsI BbvCI BcgI BciVI BclI BfaI BfrI BglII BlnI BlpI Bpu1102I BpuAI BsaAI BsaBI BsaHI BsiEI BsiWI BsmBI BsmI BsoBI Bsp1407I BspDI BspHI BspLU11I BsrBI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstF5I BstPI BstUI BstXI BstZ17I Bsu36I CelII Cfr10I ClaI CpoI DdeI DraI DraII DraIII DrdI DsaI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRV EcoT14I EcoT22I FauI FbaI FokI FseI FspI HgaI Hin1I HincII HindII HindIII HpaI HphI KasI KpnI KspI MaeI MaeII MamI MfeI MluI MseI MslI MunI MvnI NaeI NarI NcoI NdeI NgoMIV NheI NlaIII NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsaI RsrII SacII SalI SapI SbfI ScaI SexAI SfcI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StyI SwaI TaqI TfiI Tru9I Tsp509I TspRI Tth111I TthHB8I Van91I XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 41 enzymes) # ################################### AccIII 231 TCCGGA T^CCGGA AluI 224 AGCT AG^CT AspHI 223 GAGCTC G[AT]GC[AT]^C BalI 54 TGGCCA TGG^CCA BamHI 192 GGATCC G^GATCC BglI 22 GCCCTATTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BpmI 199 CTGGAG CTGGAG(16/14) BsaI 141 GAGACC GGTCTC(1/5) BsaWI 231 TCCGGA [AT]^CCGG[AT] BseAI 231 TCCGGA T^CCGGA BseRI 16 CTCCTC GAGGAG(10/8) BsiHKAI 223 GAGCTC G[AT]GC[AT]^C BsiYI 183 CCCCTACCCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BslI 183 CCCCTACCCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BsmAI 141 GAGAC GTCTC(1/5) BsmFI 181 GTCCC GGGAC(10/14) BspEI 231 TCCGGA T^CCGGA BspMI 61 GCAGGT ACCTGC(4/8) BsrI 178 CCAGT ACTGG(1/-1) BstYI 192 GGATCC [GA]^GATC[CT] Cac8I 52 GCTGGC GC[ACGT]^[ACGT]GC DpnII 193 GATC ^GATC EarI 138 GAAGAG CTCTTC(1/4) HinfI 85 GAGTC G^A[ACGT]TC Ksp632I 138 GAAGAG CTCTTC(1/4) MaeIII 239 GTGAC ^GT[ACGT]AC MboI 193 GATC ^GATC MboII 138 GAAGA GAAGA(8/7) MflI 192 GGATCC [GA]^GATC[CT] MluNI 54 TGGCCA TGG^CCA MroI 231 TCCGGA T^CCGGA MscI 54 TGGCCA TGG^CCA MspA1I 75 CAGCGG C[AC]G^C[GT]G NdeII 193 GATC ^GATC PleI 85 GAGTC GAGTC(4/5) SacI 223 GAGCTC GAGCT^C Sau3AI 193 GATC ^GATC SfaNI 47 GCATC GCATC(5/9) StuI 272 AGGCCT AGG^CCT Tsp45I 239 GTGAC ^GT[GC]AC XhoII 192 GGATCC [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 14 enzymes) # ################################### AciI 38 CCGC CCGC(-3/-1) 77 GCGG AlwI 193 GATCC GGATC(4/5) 192 GGATC BbvI 100 GCAGC GCAGC(8/12) 125 GCAGC BcnI 189 CCCGG CC^[GC]GG 294 CCCGG BsgI 80 GTGCAG GTGCAG(16/14) 98 GTGCAG CfoI 150 GCGC GCG^C 282 GCGC Cfr13I 30 GGCCC G^G[ACGT]CC 292 GGCCC EaeI 35 TGGCCG [CT]^GGCC[GA] 54 TGGCCA HaeII 149 GGCGCT [GA]GCGC^[CT] 281 GGCGCT HhaI 150 GCGC GCG^C 282 GCGC HinP1I 150 GCGC G^CGC 282 GCGC NciI 189 CCCGG CC^[GC]GG 294 CCCGG Sau96I 30 GGCCC G^G[ACGT]CC 292 GGCCC TseI 100 GCAGC G^C[AT]GC 125 GCAGC ######################################### # 3 OR MORE CUT ENZYMES ( 18 enzymes) # ######################################### BanII BmyI BsaJI Bsp1286I BssKI BstNI EcoRII Fnu4HI HaeIII HapII HpaII ItaI MnlI MspI MvaI MwoI NlaIV ScrFI