(Linear) (Six-base) MAPSORT of: KIEE5145.seq Check: 9415 from: 1 to: 300 KIEE5145.orf With 182 enzymes: SgfI * December 5, 2014 12:59 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 52 300 Size: 52 248 BamHI G'GATC_C Cuts at: 0 192 300 Size: 192 108 BanII G_rGCy'C Cuts at: 0 9 177 206 227 300 Size: 9 168 29 21 73 Fragments arranged by size: 168 73 29 21 9 BglI GCCn_nnn'nGGC Cuts at: 0 28 300 Size: 28 272 BmrI ACTGGGnnnn_n' Cuts at: 0 172 300 Size: 172 128 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 220 300 Size: 220 80 BsaI GGTCTCn'nnnn_ Cuts at: 0 135 300 Size: 135 165 BsaWI w'CCGG_w Cuts at: 0 231 300 Size: 231 69 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 164 194 300 Size: 164 30 106 Fragments arranged by size: 164 106 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 7 300 Size: 7 293 BseYI C'CCAG_C Cuts at: 0 73 168 300 Size: 73 95 132 Fragments arranged by size: 132 95 73 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 101 119 300 Size: 101 18 181 Fragments arranged by size: 181 101 18 BsiHKAI G_wGCw'C Cuts at: 0 227 300 Size: 227 73 Bsp1286I G_dGCh'C Cuts at: 0 9 177 206 227 300 Size: 9 168 29 21 73 Fragments arranged by size: 168 73 29 21 9 BspEI T'CCGG_A Cuts at: 0 231 300 Size: 231 69 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 52 300 Size: 52 248 BstYI r'GATC_y Cuts at: 0 192 300 Size: 192 108 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 33 300 Size: 33 267 EaeI y'GGCC_r Cuts at: 0 35 54 300 Size: 35 19 246 Fragments arranged by size: 246 35 19 EarI CTCTTCn'nnn_ Cuts at: 0 133 300 Size: 133 167 EcoICRI GAG'CTC Cuts at: 0 225 300 Size: 225 75 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 220 300 Size: 220 80 HaeII r_GCGC'y Cuts at: 0 153 285 300 Size: 153 132 15 Fragments arranged by size: 153 132 15 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 164 196 300 Size: 164 32 104 Fragments arranged by size: 164 104 32 MscI TGG'CCA Cuts at: 0 56 300 Size: 56 244 MspA1I CmG'CkG Cuts at: 0 77 300 Size: 77 223 SacI G_AGCT'C Cuts at: 0 227 300 Size: 227 73 StuI AGG'CCT Cuts at: 0 274 300 Size: 274 26 Enzymes that do cut: AarI BamHI BanII BglI BmrI BpmI BsaI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI Bsp1286I BspEI BspMI BstYI BtgZI EaeI EarI EcoICRI Eco57MI HaeII Hin4I MscI MspA1I SacI StuI Enzymes that do not cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AscI AseI AsiSI AvaI AvrII BaeI BanI BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglII BlpI Bme1580I BmgBI BmtI BplI Bpu10I BpuEI BsaAI BsaBI BsaHI BsiEI BsiWI BsmI BsmBI BspHI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstZ17I Bsu36I BtgI BtsI ClaI CspCI DraI DraIII DrdI EagI EciI EcoNI EcoO109I EcoRI EcoRV FalI FseI FspI FspAI HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MslI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI PacI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI PstI PvuI PvuII RsrII SacII SalI SanDI SapI SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StyI SwaI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI